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The map label for this gene is iolD [H]

Identifier: 238027781

GI number: 238027781

Start: 2403243

End: 2405255

Strand: Direct

Name: iolD [H]

Synonym: bglu_1g22050

Alternate gene names: 238027781

Gene position: 2403243-2405255 (Clockwise)

Preceding gene: 238027780

Following gene: 238027782

Centisome position: 61.52

GC content: 75.11

Gene sequence:

>2013_bases
ATGAACCATCGACAGATCGAAGCCGGCCTCGCGCCGGCCGGTGCCGCGACGGGCGGCACCGTGCGCCTGACCACCGCGCA
GGCGCTGGTCCGCTATCTGGCCGCGCAGCGCGTGGCCGCCGAGCCGGACGGGGGCCGCAGTGACCGCAGTGACCGCAGTG
GCGATACGGCGCCGCTGTTCGGCGGCGTGTTCGCGATCTTCGGCCACGGCAACGTGGCCGGGCTCGGCGAGGCGCTCTAC
CACGCGCGCGAGCTGCTGCCCACCTATCGCGCCCACAACGAGCAGGCCATGGCGCACAGCGCGATCGCCTACGCCAAGGC
GCATTTCCGGCGCCGCATGATGGCCGTGACGACCTCGATCGGCCCCGGCGCGACCAACCTCGTCACGGCCGCCGCGCTCG
CGCACGTGAACCGCCTGCCGGTGCTGCTGCTGCCCGGCGACGGCTTCGTCTCGCGCGCGCCCGACCCGGTGCTGCAGCAG
TTGGAGGATGCGCACGACGGCGGCGTGTCCGTCAACGACGCGCTGAAGGCGGTGTCGCGCTACTTCGACCGGATCGTCCA
CCCGGCGCAGCTGCTCACCGCGCTGCCGCGCGCGCTGCGCGTGCTGACCGACGCGGCGCAGTGCGGGCCGGTCACGCTCG
CGCTGCCGCAGGACGTGCAGGCGATGGCCTACGACTATCCGGCCGCGTTCTTCGCGCCGCGCGTGGTCGGCTTCCATGCG
CCGGCGCCCGTCGAGGCCGAGCTCGCGGCCGCAGCCGGCGCGCTGCGCGCCGCGCGCCGGCCGCTGCTGATCGCGGGCGG
CGGCGTGCTCTACAGCGCGGGCGGCGCGCCGGCGCTGCGCGCGTTCGCCGAGCGCCACGGCATTCCGGTCGCCGAGACCC
AGGCCGGCAAGAGCACGCTGGCCTGGGACCATCCGCTCAACGCCGGCGCGCTCGGCGTGACCGGCGCGCCCGCCGCCAAC
GCGCTGGCCGCCGAGGCCGACTGCGTGATCGCGGTCGGCACGCGGCTGCAGGACTTCACCACCGGCTCGAACACGCTGTT
CGCGCGGGCGGCCGTGATCGGCATCAACGCGAACGCGTTCGACGCGCTCAAGCACCAGGCCACCGCCGTCGCGTCCGACG
CGCGGCTCGCGCTCGATGCGCTCGGCGCCAGGCTCGAGGGCTGGCGGGCCGAGCCGGGCTGGACCACGCGCGCGCAGACG
CTCGCCGCCGGCTGGCGCGACGCCGTGCAGACGCTCACGAACACGCCGCCGCGGGCGCATGCGCTGCCCTACGACGCCGA
CGTGATCGGCGCCGTGCAGCGCAGCAGCGCCGCCTCGGCGGCCGGCGACATCGCCGTGTGCGCGGCCGGCACGCTGCCCG
CCGAGCTGCACAAGCTCTGGCGCGCCGCGGTGCCGGGCGGCTATCACGTCGAATACGGCTACTCCTGCATGGGCTACGAG
ATCGCGGGCGGCCTCGGCGTGAAGCTCGCGCGGCCCGAGCGCGAGGTGATCGTGTTCGTCGGCGACGGCAGCTACCTGAT
GATGAACAGCGAGATCGCCACCTCCGTGATGCTCGGCGCGAAGCTGATCGTGGTGGTGCTCGACAACCGCGGCTACGGCT
GCATCAACCGGCTCCAGCAGGCCTGCGGCGGCGCGCCGTTCAACAACCTGCTCGACGATTGCCTGCAAGGCCCGGCAGGC
GCGCCCGCGATCGACTTCGCCGCGCATGCGCGCGCGCTCGGCGCCCACGGCGAGCACGTGGCCGACCTGGCCGCGCTCGA
CGCCGCGCTGGCCCGCGCCCGCGCGGCCGAACGCACCAGCGTGATCTGCATCGACACCGATCCGGCGCGCACCACCGACG
ACGGCGGCTGGTGGTGGGAGGTGGCCGTGCCCGAAGTCTCGCCGCGCGCCGCCGTGCGCGAGGCGCGGGCGAACTACGAA
GCACGGCTCGCCGCCCGCGCCGGCGCCTCCGGTGCCGGCCTGCCCGCCGCCGGCTGCACCGCTGAACCCCACTCCCCGGA
ATCATCGACATGA

Upstream 100 bases:

>100_bases
TCGCGCGCGTGCGCGCCACCTTCGAGACGCTGATCGACGCCTGGCGCACGGCGCGCGCCGGCCGCGTCGCCGCGCGCCGG
GTCGCATGAGGAGCCCCCCG

Downstream 100 bases:

>100_bases
GTTACGACATCCGAATCGGCATCAACCCGCTGTCGTGGATGAACGACGACCTGCCTTCGCTCGGCGGCGAGACCCCGCTG
GCCGTGGCGCTGACCGAAGG

Product: IolD protein

Products: pyruvate; 2-aceto-2-hydroxy-butyrate; CO2 [C]

Alternate protein names: THcHDO hydrolase [H]

Number of amino acids: Translated: 670; Mature: 670

Protein sequence:

>670_residues
MNHRQIEAGLAPAGAATGGTVRLTTAQALVRYLAAQRVAAEPDGGRSDRSDRSGDTAPLFGGVFAIFGHGNVAGLGEALY
HARELLPTYRAHNEQAMAHSAIAYAKAHFRRRMMAVTTSIGPGATNLVTAAALAHVNRLPVLLLPGDGFVSRAPDPVLQQ
LEDAHDGGVSVNDALKAVSRYFDRIVHPAQLLTALPRALRVLTDAAQCGPVTLALPQDVQAMAYDYPAAFFAPRVVGFHA
PAPVEAELAAAAGALRAARRPLLIAGGGVLYSAGGAPALRAFAERHGIPVAETQAGKSTLAWDHPLNAGALGVTGAPAAN
ALAAEADCVIAVGTRLQDFTTGSNTLFARAAVIGINANAFDALKHQATAVASDARLALDALGARLEGWRAEPGWTTRAQT
LAAGWRDAVQTLTNTPPRAHALPYDADVIGAVQRSSAASAAGDIAVCAAGTLPAELHKLWRAAVPGGYHVEYGYSCMGYE
IAGGLGVKLARPEREVIVFVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNLLDDCLQGPAG
APAIDFAAHARALGAHGEHVADLAALDAALARARAAERTSVICIDTDPARTTDDGGWWWEVAVPEVSPRAAVREARANYE
ARLAARAGASGAGLPAAGCTAEPHSPESST

Sequences:

>Translated_670_residues
MNHRQIEAGLAPAGAATGGTVRLTTAQALVRYLAAQRVAAEPDGGRSDRSDRSGDTAPLFGGVFAIFGHGNVAGLGEALY
HARELLPTYRAHNEQAMAHSAIAYAKAHFRRRMMAVTTSIGPGATNLVTAAALAHVNRLPVLLLPGDGFVSRAPDPVLQQ
LEDAHDGGVSVNDALKAVSRYFDRIVHPAQLLTALPRALRVLTDAAQCGPVTLALPQDVQAMAYDYPAAFFAPRVVGFHA
PAPVEAELAAAAGALRAARRPLLIAGGGVLYSAGGAPALRAFAERHGIPVAETQAGKSTLAWDHPLNAGALGVTGAPAAN
ALAAEADCVIAVGTRLQDFTTGSNTLFARAAVIGINANAFDALKHQATAVASDARLALDALGARLEGWRAEPGWTTRAQT
LAAGWRDAVQTLTNTPPRAHALPYDADVIGAVQRSSAASAAGDIAVCAAGTLPAELHKLWRAAVPGGYHVEYGYSCMGYE
IAGGLGVKLARPEREVIVFVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNLLDDCLQGPAG
APAIDFAAHARALGAHGEHVADLAALDAALARARAAERTSVICIDTDPARTTDDGGWWWEVAVPEVSPRAAVREARANYE
ARLAARAGASGAGLPAAGCTAEPHSPESST
>Mature_670_residues
MNHRQIEAGLAPAGAATGGTVRLTTAQALVRYLAAQRVAAEPDGGRSDRSDRSGDTAPLFGGVFAIFGHGNVAGLGEALY
HARELLPTYRAHNEQAMAHSAIAYAKAHFRRRMMAVTTSIGPGATNLVTAAALAHVNRLPVLLLPGDGFVSRAPDPVLQQ
LEDAHDGGVSVNDALKAVSRYFDRIVHPAQLLTALPRALRVLTDAAQCGPVTLALPQDVQAMAYDYPAAFFAPRVVGFHA
PAPVEAELAAAAGALRAARRPLLIAGGGVLYSAGGAPALRAFAERHGIPVAETQAGKSTLAWDHPLNAGALGVTGAPAAN
ALAAEADCVIAVGTRLQDFTTGSNTLFARAAVIGINANAFDALKHQATAVASDARLALDALGARLEGWRAEPGWTTRAQT
LAAGWRDAVQTLTNTPPRAHALPYDADVIGAVQRSSAASAAGDIAVCAAGTLPAELHKLWRAAVPGGYHVEYGYSCMGYE
IAGGLGVKLARPEREVIVFVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQACGGAPFNNLLDDCLQGPAG
APAIDFAAHARALGAHGEHVADLAALDAALARARAAERTSVICIDTDPARTTDDGGWWWEVAVPEVSPRAAVREARANYE
ARLAARAGASGAGLPAAGCTAEPHSPESST

Specific function: Involved in the cleavage of the C1-C2 bond of 3D- (3,5/4)-trihydroxycyclohexane-1,2-dione (THcHDO) to yield 5-deoxy- glucuronate (5DG) [H]

COG id: COG3962

COG function: function code E; Acetolactate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1790104, Length=431, Percent_Identity=27.1461716937355, Blast_Score=123, Evalue=4e-29,
Organism=Escherichia coli, GI87081685, Length=562, Percent_Identity=25.9786476868327, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI1786717, Length=512, Percent_Identity=25.1953125, Blast_Score=96, Evalue=5e-21,
Organism=Escherichia coli, GI1787096, Length=481, Percent_Identity=23.7006237006237, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17531299, Length=528, Percent_Identity=24.2424242424242, Blast_Score=66, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17531301, Length=528, Percent_Identity=24.2424242424242, Blast_Score=66, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6323755, Length=573, Percent_Identity=23.9092495636998, Blast_Score=119, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 2.2.1.6 [C]

Molecular weight: Translated: 69207; Mature: 69207

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHRQIEAGLAPAGAATGGTVRLTTAQALVRYLAAQRVAAEPDGGRSDRSDRSGDTAPLF
CCCCEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
GGVFAIFGHGNVAGLGEALYHARELLPTYRAHNEQAMAHSAIAYAKAHFRRRMMAVTTSI
HCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECC
GPGATNLVTAAALAHVNRLPVLLLPGDGFVSRAPDPVLQQLEDAHDGGVSVNDALKAVSR
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHH
YFDRIVHPAQLLTALPRALRVLTDAAQCGPVTLALPQDVQAMAYDYPAAFFAPRVVGFHA
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHCCCHHHHHHHHEECCC
PAPVEAELAAAAGALRAARRPLLIAGGGVLYSAGGAPALRAFAERHGIPVAETQAGKSTL
CCCCHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCHHHHHHHHHCCCCCEECCCCCCCC
AWDHPLNAGALGVTGAPAANALAAEADCVIAVGTRLQDFTTGSNTLFARAAVIGINANAF
CCCCCCCCCCCCCCCCCCCCHHHCCCCEEEEECCCHHCCCCCCCCEEEEEEEEECCCCHH
DALKHQATAVASDARLALDALGARLEGWRAEPGWTTRAQTLAAGWRDAVQTLTNTPPRAH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
ALPYDADVIGAVQRSSAASAAGDIAVCAAGTLPAELHKLWRAAVPGGYHVEYGYSCMGYE
CCCCCHHHHHHHHHHCHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCEEECEE
IAGGLGVKLARPEREVIVFVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQ
ECCCCCEEEECCCCEEEEEECCCCEEEECHHHHHHHHHCCEEEEEEECCCCCHHHHHHHH
ACGGAPFNNLLDDCLQGPAGAPAIDFAAHARALGAHGEHVADLAALDAALARARAAERTS
HHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCE
VICIDTDPARTTDDGGWWWEVAVPEVSPRAAVREARANYEARLAARAGASGAGLPAAGCT
EEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCC
AEPHSPESST
CCCCCCCCCC
>Mature Secondary Structure
MNHRQIEAGLAPAGAATGGTVRLTTAQALVRYLAAQRVAAEPDGGRSDRSDRSGDTAPLF
CCCCEECCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHH
GGVFAIFGHGNVAGLGEALYHARELLPTYRAHNEQAMAHSAIAYAKAHFRRRMMAVTTSI
HCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHEECC
GPGATNLVTAAALAHVNRLPVLLLPGDGFVSRAPDPVLQQLEDAHDGGVSVNDALKAVSR
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHHHHHH
YFDRIVHPAQLLTALPRALRVLTDAAQCGPVTLALPQDVQAMAYDYPAAFFAPRVVGFHA
HHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHHHHHCCCHHHHHHHHEECCC
PAPVEAELAAAAGALRAARRPLLIAGGGVLYSAGGAPALRAFAERHGIPVAETQAGKSTL
CCCCHHHHHHHHHHHHHHCCCEEEECCCEEEECCCCHHHHHHHHHCCCCCEECCCCCCCC
AWDHPLNAGALGVTGAPAANALAAEADCVIAVGTRLQDFTTGSNTLFARAAVIGINANAF
CCCCCCCCCCCCCCCCCCCCHHHCCCCEEEEECCCHHCCCCCCCCEEEEEEEEECCCCHH
DALKHQATAVASDARLALDALGARLEGWRAEPGWTTRAQTLAAGWRDAVQTLTNTPPRAH
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCC
ALPYDADVIGAVQRSSAASAAGDIAVCAAGTLPAELHKLWRAAVPGGYHVEYGYSCMGYE
CCCCCHHHHHHHHHHCHHCCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCEEECEE
IAGGLGVKLARPEREVIVFVGDGSYLMMNSEIATSVMLGAKLIVVVLDNRGYGCINRLQQ
ECCCCCEEEECCCCEEEEEECCCCEEEECHHHHHHHHHCCEEEEEEECCCCCHHHHHHHH
ACGGAPFNNLLDDCLQGPAGAPAIDFAAHARALGAHGEHVADLAALDAALARARAAERTS
HHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCE
VICIDTDPARTTDDGGWWWEVAVPEVSPRAAVREARANYEARLAARAGASGAGLPAAGCT
EEEEECCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCC
AEPHSPESST
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 4002 [C]

Specific activity: 53

Km value (mM): 5 {2-oxobutanoate}} 1 {2-oxobutanoate}} 7.6 {pyruvate}} 7 {pyruvate}} 2.9 {pyruvate}} 2.6 {pyruvate}} 1.3 {pyruvate}} 1 {pyruvate}} [C]

Substrates: acetolactate; CO2; pyruvate; 2-oxobutanoate [C]

Specific reaction: acetolactate + CO2 = 2 pyruvate pyruvate + 2-oxobutanoate = 2-aceto-2-hydroxy-butyrate + CO2 [C]

General reaction: C-C bond formation; decarboxylation [C]

Inhibitor: @OXOB02.txt*2-oxobutanoate!; chlorimuronethyl; chlorsulphuron; Ile; imazapyr; Leu; Val [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA