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The map label for this gene is tfdA [H]

Identifier: 238027309

GI number: 238027309

Start: 1883534

End: 1884379

Strand: Reverse

Name: tfdA [H]

Synonym: bglu_1g16970

Alternate gene names: 238027309

Gene position: 1884379-1883534 (Counterclockwise)

Preceding gene: 238027310

Following gene: 238027300

Centisome position: 48.24

GC content: 63.83

Gene sequence:

>846_bases
ATGAAAGTCATTCCCTTGTCACCGGCGCTCGGTGCCGAAGTTCGCGGCATCGACCTGACCCGCCCGTTGTCCGCGCGCAC
GCGCGACGATATCGTCGGCCTGCTCACCGAGCATCAGATGCTGGTGTTTCCCGACATGTCCATGTCGAGCGAGCAGCAAA
TCGCGATGAGCCGCAATTTCGGCGAGCTCGAGCCGCATCCGATGACGACCAACACCTCGCCCTACCCGGAAATAACGGTG
GTCTCGAACGTGACCACCGACGGCAAGCCGCTCGGTTATCCGACGCCGCCGTTCCAGCTCTGGCATTCCGACCTCTGCTA
CCTGGAGCGGCCCGCGAAGATGACGCTGTTCTATGCCGAGTCGGTGCCCGAGCAGCATGGCGACACCTGGTTCGCCAACA
TGTTTCGCGCCTACGACACGCTGCCCGAGCACCTGAAGGCCGCGCTCGACGGCCGCAATGCCGTCTTCAGCCTGGACGGC
AGCCTGGTGAAGCGCTGCCGCAAGATCGGCTTCGACCTCAACATCGCCGCCGACGACTTCAAGCCGACCGTCTCGCATCC
CGCCATCCGCGTCCACCCGGAGTCGCTGCGCCGCTCGATCTTCGTCAACTGGGCCCATACCGACAGCGTGGAGGGGTATT
CGCCCGAGCAGAGCGAGGCGCTGCTGGAGCAGGTCTTCGCGCACTGCCTGAACGAGGACTTCATCTACCGTCACCGCTAC
ACGGCCGGTGAACTGGTGATCTGGGACAACGCCTCGGTGATCCACACCAATTCCCCGCACGTCCCGGTCGGCAATCGCAT
CATGCGCCGCGTGATGGTGACCGGACAGGCTCCGTTCTTCCAGTAA

Upstream 100 bases:

>100_bases
CGGCCAGCCCGGCTCCGCCCCGCTGCGTCGCGCGCCGTGCCAGATCCTTGTCCCAGCTGTTTCTCCCCCTCCTGCCTCAT
CTGCCAGCCAGGATTCCCGA

Downstream 100 bases:

>100_bases
TCGGGCCGCCAGACCGGCGTCCGATACCCGGGCGCCGGGCCGTGTTCGCTGCCGGCCCGGCGCCCCTTCCCTTCCCGTCC
CGCCTTTGCCCTTCCTCCCA

Product: TauD/TfdA family dioxygenase

Products: Aminoacetaldehyde; CO2; H+; Sulfite; Succinate [C]

Alternate protein names: 2,4-D dioxygenase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MKVIPLSPALGAEVRGIDLTRPLSARTRDDIVGLLTEHQMLVFPDMSMSSEQQIAMSRNFGELEPHPMTTNTSPYPEITV
VSNVTTDGKPLGYPTPPFQLWHSDLCYLERPAKMTLFYAESVPEQHGDTWFANMFRAYDTLPEHLKAALDGRNAVFSLDG
SLVKRCRKIGFDLNIAADDFKPTVSHPAIRVHPESLRRSIFVNWAHTDSVEGYSPEQSEALLEQVFAHCLNEDFIYRHRY
TAGELVIWDNASVIHTNSPHVPVGNRIMRRVMVTGQAPFFQ

Sequences:

>Translated_281_residues
MKVIPLSPALGAEVRGIDLTRPLSARTRDDIVGLLTEHQMLVFPDMSMSSEQQIAMSRNFGELEPHPMTTNTSPYPEITV
VSNVTTDGKPLGYPTPPFQLWHSDLCYLERPAKMTLFYAESVPEQHGDTWFANMFRAYDTLPEHLKAALDGRNAVFSLDG
SLVKRCRKIGFDLNIAADDFKPTVSHPAIRVHPESLRRSIFVNWAHTDSVEGYSPEQSEALLEQVFAHCLNEDFIYRHRY
TAGELVIWDNASVIHTNSPHVPVGNRIMRRVMVTGQAPFFQ
>Mature_281_residues
MKVIPLSPALGAEVRGIDLTRPLSARTRDDIVGLLTEHQMLVFPDMSMSSEQQIAMSRNFGELEPHPMTTNTSPYPEITV
VSNVTTDGKPLGYPTPPFQLWHSDLCYLERPAKMTLFYAESVPEQHGDTWFANMFRAYDTLPEHLKAALDGRNAVFSLDG
SLVKRCRKIGFDLNIAADDFKPTVSHPAIRVHPESLRRSIFVNWAHTDSVEGYSPEQSEALLEQVFAHCLNEDFIYRHRY
TAGELVIWDNASVIHTNSPHVPVGNRIMRRVMVTGQAPFFQ

Specific function: Involved in degradation of the herbicide 2,4- dichlorophenoxyacetic acid (2,4-D). Is also able to degrade 2- methyl-4-chlorophenoxyacetic acid and 3-chlorobenzoic acid [H]

COG id: COG2175

COG function: function code Q; Probable taurine catabolism dioxygenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tfdA dioxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1786565, Length=285, Percent_Identity=30.5263157894737, Blast_Score=139, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6322971, Length=271, Percent_Identity=27.3062730627306, Blast_Score=91, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003819 [H]

Pfam domain/function: PF02668 TauD [H]

EC number: 1.14.11.17 [C]

Molecular weight: Translated: 31736; Mature: 31736

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVIPLSPALGAEVRGIDLTRPLSARTRDDIVGLLTEHQMLVFPDMSMSSEQQIAMSRNF
CEEECCCCCCCCEECCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCC
GELEPHPMTTNTSPYPEITVVSNVTTDGKPLGYPTPPFQLWHSDLCYLERPAKMTLFYAE
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCHHEECCCCEEEEEEEC
SVPEQHGDTWFANMFRAYDTLPEHLKAALDGRNAVFSLDGSLVKRCRKIGFDLNIAADDF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCEEEECCCC
KPTVSHPAIRVHPESLRRSIFVNWAHTDSVEGYSPEQSEALLEQVFAHCLNEDFIYRHRY
CCCCCCCEEEECHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
TAGELVIWDNASVIHTNSPHVPVGNRIMRRVMVTGQAPFFQ
CCCCEEEECCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKVIPLSPALGAEVRGIDLTRPLSARTRDDIVGLLTEHQMLVFPDMSMSSEQQIAMSRNF
CEEECCCCCCCCEECCCCCCCCCCCCCHHHHHHHHCCCEEEEECCCCCCCCHHHHHHCCC
GELEPHPMTTNTSPYPEITVVSNVTTDGKPLGYPTPPFQLWHSDLCYLERPAKMTLFYAE
CCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHCCHHEECCCCEEEEEEEC
SVPEQHGDTWFANMFRAYDTLPEHLKAALDGRNAVFSLDGSLVKRCRKIGFDLNIAADDF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCEEEECCCC
KPTVSHPAIRVHPESLRRSIFVNWAHTDSVEGYSPEQSEALLEQVFAHCLNEDFIYRHRY
CCCCCCCEEEECHHHHCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEC
TAGELVIWDNASVIHTNSPHVPVGNRIMRRVMVTGQAPFFQ
CCCCEEEECCCEEEECCCCCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Ascorbate. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-Oxoglutarate; O2; Taurine [C]

Specific reaction: 2-Oxoglutarate + O2 + Taurine --> Aminoacetaldehyde + CO2 + H+ + Sulfite + Succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3036764; 10777523; 11457355; 11955067 [H]