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The map label for this gene is rlmN [H]

Identifier: 238027069

GI number: 238027069

Start: 1593058

End: 1594194

Strand: Direct

Name: rlmN [H]

Synonym: bglu_1g14480

Alternate gene names: 238027069

Gene position: 1593058-1594194 (Clockwise)

Preceding gene: 238027068

Following gene: 238027070

Centisome position: 40.78

GC content: 65.35

Gene sequence:

>1137_bases
ATGACGAGCGATACTCCCGTCAATCTCCTCGACTTCGACGCCGAAGGTCTCGTCGCGTATTGCGGCAGCCTCGGCGAGAA
GCCGTTTCGCGCCAAGCAATTGCAGCGCTGGATCCATCAATACAATGCCGCCGATTTCGACGGCATGACCGATCTCGCGA
AGTCGTTGCGGGAAAAGTTGAAAGGGCGTGCCGTGATCGGCATGCCCGAGATCGCGAGCGACCATGTCTCGTCGGACGGC
ACGCGCAAGTGGCTGATCGACGTGGGCAACGGCAACGCGGTCGAGACCGTCTTCATTCCGGAGGAGACGCGCGGCACGCT
GTGCGTGTCCTCGCAGGCCGGATGCGCGGTCAACTGCCGGTTCTGTTCGACGGGCAAGCAGGGTTTTTCGCGCAACCTGT
CCACGGCTGAAATCGTTGGGCAGTTGCGGATGGCCGAATTCGCGTTGCGCGAATCGCTCGGGCGCGCGCCCGGGCCGAAC
GGCAAGGCCGAGCGCGTCGTCACGAACGTCGTGATGATGGGCATGGGCGAGCCGCTTCTCAATTACAGCGCGGTGGTGCC
GGCCATGCGGCTGATGCTCGACGACAACGCCTACGGGCTGTCGCGGCGCCGCGTGACGCTGTCGACTTCCGGCGTCGTGC
CGATGATGGACCGCCTCGGCGCGGAACTGCCGGTCGCGCTGGCGGTGTCGCTGCACGCACCGAACGATGCGCTGCGCGAC
ATGCTCGTGCCACTCAACAAGAAGTACCCGCTGCGTGAGCTGATGGCGGCGTGTCAGCGCTACCTGGCCGTCGCGCCGCG
GGATTTCATCACATTCGAATACTGCATGCTCGACGGCATCAACGACACCGACGCGCATGCGCGCGAGCTGCTCGCCGTGA
CGCGCGACGTGCCGTGCAAGTTCAACCTGATCCCGTTCAATCCGTTCCCCGAGTCGGGGCTGCTGCGCTCGAAGAACGAG
CGCATCAAGCAGTTCTCGCAGATCCTGATCGAGGCGGGCGTCGTGACGACGGTGCGCAAGACGCGCGGCGACGACATCGA
TGCGGCCTGCGGCCAGCTCGCCGGCGCCGTGAAGGACCGCACGCGCCTACAGCAGCGCACGGGAAGCGGGAAGGTGATCG
AAGTCCGGGCCGTCTGA

Upstream 100 bases:

>100_bases
GAAACGGCGGCCGTCGAAATCGGCTTCTTCTTCGCAGGTCTGAACGTCTACTCGCGCTAAGCGGGTAGCGGGTCCGGCGC
GTGCGGCAAGGCAGGGCACC

Downstream 100 bases:

>100_bases
CGGGGAGGTCCGGGCGGGTAGTGGGTAGACGCGACAAGCGATCCGGGGCGGGTCGGGCCGCCCCTGTTTAACGGTTGTGC
TGCGGCGCCGGGCCATGTTC

Product: radical SAM superfamily protein

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTSDTPVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAADFDGMTDLAKSLREKLKGRAVIGMPEIASDHVSSDG
TRKWLIDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTAEIVGQLRMAEFALRESLGRAPGPN
GKAERVVTNVVMMGMGEPLLNYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
MLVPLNKKYPLRELMAACQRYLAVAPRDFITFEYCMLDGINDTDAHARELLAVTRDVPCKFNLIPFNPFPESGLLRSKNE
RIKQFSQILIEAGVVTTVRKTRGDDIDAACGQLAGAVKDRTRLQQRTGSGKVIEVRAV

Sequences:

>Translated_378_residues
MTSDTPVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAADFDGMTDLAKSLREKLKGRAVIGMPEIASDHVSSDG
TRKWLIDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTAEIVGQLRMAEFALRESLGRAPGPN
GKAERVVTNVVMMGMGEPLLNYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
MLVPLNKKYPLRELMAACQRYLAVAPRDFITFEYCMLDGINDTDAHARELLAVTRDVPCKFNLIPFNPFPESGLLRSKNE
RIKQFSQILIEAGVVTTVRKTRGDDIDAACGQLAGAVKDRTRLQQRTGSGKVIEVRAV
>Mature_377_residues
TSDTPVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAADFDGMTDLAKSLREKLKGRAVIGMPEIASDHVSSDGT
RKWLIDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCRFCSTGKQGFSRNLSTAEIVGQLRMAEFALRESLGRAPGPNG
KAERVVTNVVMMGMGEPLLNYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRDM
LVPLNKKYPLRELMAACQRYLAVAPRDFITFEYCMLDGINDTDAHARELLAVTRDVPCKFNLIPFNPFPESGLLRSKNER
IKQFSQILIEAGVVTTVRKTRGDDIDAACGQLAGAVKDRTRLQQRTGSGKVIEVRAV

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA [H]

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family [H]

Homologues:

Organism=Escherichia coli, GI1788865, Length=380, Percent_Identity=53.9473684210526, Blast_Score=401, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004383 [H]

Pfam domain/function: NA

EC number: =2.1.1.192 [H]

Molecular weight: Translated: 41355; Mature: 41224

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDTPVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAADFDGMTDLAKSLREKL
CCCCCCCCEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGRAVIGMPEIASDHVSSDGTRKWLIDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCR
CCCEEECCCCHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEE
FCSTGKQGFSRNLSTAEIVGQLRMAEFALRESLGRAPGPNGKAERVVTNVVMMGMGEPLL
EECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHH
NYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEECCCHHHHH
MLVPLNKKYPLRELMAACQRYLAVAPRDFITFEYCMLDGINDTDAHARELLAVTRDVPCK
HHCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEHEECCCCCCHHHHHHHHHHHCCCCCE
FNLIPFNPFPESGLLRSKNERIKQFSQILIEAGVVTTVRKTRGDDIDAACGQLAGAVKDR
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TRLQQRTGSGKVIEVRAV
HHHHHHCCCCCEEEEEEC
>Mature Secondary Structure 
TSDTPVNLLDFDAEGLVAYCGSLGEKPFRAKQLQRWIHQYNAADFDGMTDLAKSLREKL
CCCCCCCEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KGRAVIGMPEIASDHVSSDGTRKWLIDVGNGNAVETVFIPEETRGTLCVSSQAGCAVNCR
CCCEEECCCCHHHHHCCCCCCEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCEEEEE
FCSTGKQGFSRNLSTAEIVGQLRMAEFALRESLGRAPGPNGKAERVVTNVVMMGMGEPLL
EECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCHHH
NYSAVVPAMRLMLDDNAYGLSRRRVTLSTSGVVPMMDRLGAELPVALAVSLHAPNDALRD
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHCCCCCCEEEEEEEECCCHHHHH
MLVPLNKKYPLRELMAACQRYLAVAPRDFITFEYCMLDGINDTDAHARELLAVTRDVPCK
HHCCCCCCCCHHHHHHHHHHHHHCCCCHHHEEEEHEECCCCCCHHHHHHHHHHHCCCCCE
FNLIPFNPFPESGLLRSKNERIKQFSQILIEAGVVTTVRKTRGDDIDAACGQLAGAVKDR
EEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
TRLQQRTGSGKVIEVRAV
HHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA