Click here to switch to the map view.

The map label for this gene is dfrA [H]

Identifier: 238026572

GI number: 238026572

Start: 992671

End: 993174

Strand: Reverse

Name: dfrA [H]

Synonym: bglu_1g09160

Alternate gene names: 238026572

Gene position: 993174-992671 (Counterclockwise)

Preceding gene: 238026574

Following gene: 238026570

Centisome position: 25.42

GC content: 71.83

Gene sequence:

>504_bases
ATGACGACCCTGAGCCTGATCGTCGCGCGCGCCCGCAACGGCGTGATCGGCCGCGACAACCAGCTTCCCTGGAAGCTCCC
CGAGGATCTCGCGTTCTTCAAGCGCACCACGATGGGCGCGCCGATCGTGATGGGCCGCAAGACCCACGAATCGATCGGCA
GGCCGCTGCCGGGGCGCCGCAACATCGTCGTCACGCGCGACGCGACGCGGCGCTTCGACGGCTGCGACGTGGCCGGCTCG
CTGGCCGAGGCGCTCGCGGCGGCGGCACGCGACGGCGCGGCCGAAGCCTTCCTGATCGGCGGCGCGCAGCTGTACGCCGA
GGGGCTGCACGCCGCCGACAAGCTGATCGTCACCGAGATCGACGCCGATTTCGACGGCGACGCGCATTTCCCGGCCCCCG
ATCCGGCGCAGTGGCGCGAGGTGTCGCGCGAGCGCCATCATGCGGCCGCGCCGAACGACTTCGACTACGCGTTCGTGGTC
TACGTGCGCGCGCCTGCGCCCTGA

Upstream 100 bases:

>100_bases
GCGGCCAGGCGCCCGGCAGGCAGCGACGCGCACCCGCCGGCCGGCCCCGCCCGGCGATCCGGTAGAATCGGCGGCTTTCC
CGCCCGCCCCCGGATCTCCC

Downstream 100 bases:

>100_bases
CGAGCCCGGCCCGCTGCCTGCCTGCGCCCCCACCGCGCCGATAAAAAAAAGCGCCGCTTCGCGCGGCGCTTCCTGCTTCA
GCCCGGCGCGGGGCAAGCCC

Product: dihydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 167; Mature: 166

Protein sequence:

>167_residues
MTTLSLIVARARNGVIGRDNQLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRRNIVVTRDATRRFDGCDVAGS
LAEALAAAARDGAAEAFLIGGAQLYAEGLHAADKLIVTEIDADFDGDAHFPAPDPAQWREVSRERHHAAAPNDFDYAFVV
YVRAPAP

Sequences:

>Translated_167_residues
MTTLSLIVARARNGVIGRDNQLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRRNIVVTRDATRRFDGCDVAGS
LAEALAAAARDGAAEAFLIGGAQLYAEGLHAADKLIVTEIDADFDGDAHFPAPDPAQWREVSRERHHAAAPNDFDYAFVV
YVRAPAP
>Mature_166_residues
TTLSLIVARARNGVIGRDNQLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRRNIVVTRDATRRFDGCDVAGSL
AEALAAAARDGAAEAFLIGGAQLYAEGLHAADKLIVTEIDADFDGDAHFPAPDPAQWREVSRERHHAAAPNDFDYAFVVY
VRAPAP

Specific function: Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis [H]

COG id: COG0262

COG function: function code H; Dihydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DHFR (dihydrofolate reductase) domain [H]

Homologues:

Organism=Homo sapiens, GI4503323, Length=155, Percent_Identity=31.6129032258064, Blast_Score=70, Evalue=8e-13,
Organism=Escherichia coli, GI1786233, Length=160, Percent_Identity=45, Blast_Score=135, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17505899, Length=183, Percent_Identity=30.6010928961749, Blast_Score=77, Evalue=3e-15,
Organism=Drosophila melanogaster, GI116008026, Length=157, Percent_Identity=33.1210191082803, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24647458, Length=157, Percent_Identity=33.1210191082803, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 10,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012259
- InterPro:   IPR017925
- InterPro:   IPR001796 [H]

Pfam domain/function: PF00186 DHFR_1 [H]

EC number: =1.5.1.3 [H]

Molecular weight: Translated: 18119; Mature: 17987

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00075 DHFR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTLSLIVARARNGVIGRDNQLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRR
CCHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCC
NIVVTRDATRRFDGCDVAGSLAEALAAAARDGAAEAFLIGGAQLYAEGLHAADKLIVTEI
CEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCEEEEEEE
DADFDGDAHFPAPDPAQWREVSRERHHAAAPNDFDYAFVVYVRAPAP
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCC
>Mature Secondary Structure 
TTLSLIVARARNGVIGRDNQLPWKLPEDLAFFKRTTMGAPIVMGRKTHESIGRPLPGRR
CHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCC
NIVVTRDATRRFDGCDVAGSLAEALAAAARDGAAEAFLIGGAQLYAEGLHAADKLIVTEI
CEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHCEEEEEEE
DADFDGDAHFPAPDPAQWREVSRERHHAAAPNDFDYAFVVYVRAPAP
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2840350; 9384377 [H]