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The map label for this gene is trmD [H]

Identifier: 238026561

GI number: 238026561

Start: 983079

End: 983876

Strand: Direct

Name: trmD [H]

Synonym: bglu_1g09050

Alternate gene names: 238026561

Gene position: 983079-983876 (Clockwise)

Preceding gene: 238026560

Following gene: 238026562

Centisome position: 25.17

GC content: 67.79

Gene sequence:

>798_bases
ATGAATGACGCCGAGGCGCGCGCGATGCAGTTCGATGTCGTCACGCTCTTTCCCGAGATGTTCGCCGCGCTGACCGACTG
GGGCATTACCAGCCGGGCCGTGAAGCAGGGGCGTTTCGGGTTGCGCACGTGGAATCCGCGCGATTTCACCACGGACAACT
ACCGGACGGTCGACGACCGCCCCTACGGCGGCGGTCCCGGCATGGTGATGATCCCGAAGCCGCTCGAGGCGGCGATCGGC
GCGGCGAAGGCCGCGCAGGCCGCCCAGGGCATCGCCGCGACGCGGGTCGTGATGATGTCGCCGCAAGGCGCGCCGCTCAC
GCACGAACGCGTGATGCGGATGGTCGACGAGCCCGGTGTGGTATTGCTGTGCGGCCGCTACGAGGCGATCGACCAGCGTT
TGCTCGATCGCTGCGTGGACGAGGAAATCAGCCTCGGCGATTTCGTGCTGTCGGGCGGCGAACTGCCGGCGATGGCGCTG
ATGGATGCGGTGGTGCGCCAGTTGCCCGGCGTCCTGAATGATACGCAGTCGGCCGTGCAGGACAGTTTCGTCGACGGCCT
GCTTGATTGCCCGCATTACACGCGGCCCGAGGAGTACGAGGGCGCGCGGGTGCCCGACGTGCTGCTCGGCGGCCATCATG
CCGAGATCGAGAAGTGGCGGCGCCAGGAAGCGCTGCGCAACACGTGGCAGAAGCGCCCGGACCTGATCGCACGGGCGCGC
AGCAAGCAGTTGTTGAGCCGGGCCGACGAGGCCTGGCTGGCAAGTCTCGCGAAGGCCGCGAAGCAAGCCTCGAACTGA

Upstream 100 bases:

>100_bases
GTCACCGGCGAGCGGCTGATTCCGTTCGTCGGCGTGTACGTGAAAACGGTCGATCAGGCGGCGAAGCGCATCGTCGTCGA
CTGGGAAGCCGATTACTGAA

Downstream 100 bases:

>100_bases
GGCGTGCTCGCGGCGGACCGGGAACCAGGCGGTGGCGCGCATGCGTGCGCGGCACCAGTTGTGAATCCATCCTCTATCGG
GGCCAGGTCCGGACCGGACA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MNDAEARAMQFDVVTLFPEMFAALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMIPKPLEAAIG
AAKAAQAAQGIAATRVVMMSPQGAPLTHERVMRMVDEPGVVLLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAL
MDAVVRQLPGVLNDTQSAVQDSFVDGLLDCPHYTRPEEYEGARVPDVLLGGHHAEIEKWRRQEALRNTWQKRPDLIARAR
SKQLLSRADEAWLASLAKAAKQASN

Sequences:

>Translated_265_residues
MNDAEARAMQFDVVTLFPEMFAALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMIPKPLEAAIG
AAKAAQAAQGIAATRVVMMSPQGAPLTHERVMRMVDEPGVVLLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAL
MDAVVRQLPGVLNDTQSAVQDSFVDGLLDCPHYTRPEEYEGARVPDVLLGGHHAEIEKWRRQEALRNTWQKRPDLIARAR
SKQLLSRADEAWLASLAKAAKQASN
>Mature_265_residues
MNDAEARAMQFDVVTLFPEMFAALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDRPYGGGPGMVMIPKPLEAAIG
AAKAAQAAQGIAATRVVMMSPQGAPLTHERVMRMVDEPGVVLLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMAL
MDAVVRQLPGVLNDTQSAVQDSFVDGLLDCPHYTRPEEYEGARVPDVLLGGHHAEIEKWRRQEALRNTWQKRPDLIARAR
SKQLLSRADEAWLASLAKAAKQASN

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=228, Percent_Identity=54.8245614035088, Blast_Score=254, Evalue=5e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 29248; Mature: 29248

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDAEARAMQFDVVTLFPEMFAALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCC
PYGGGPGMVMIPKPLEAAIGAAKAAQAAQGIAATRVVMMSPQGAPLTHERVMRMVDEPGV
CCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHCCCCE
VLLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMALMDAVVRQLPGVLNDTQSAVQ
EEEECCHHHHHHHHHHHHHHCCCCCCHHEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
DSFVDGLLDCPHYTRPEEYEGARVPDVLLGGHHAEIEKWRRQEALRNTWQKRPDLIARAR
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHH
SKQLLSRADEAWLASLAKAAKQASN
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDAEARAMQFDVVTLFPEMFAALTDWGITSRAVKQGRFGLRTWNPRDFTTDNYRTVDDR
CCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCCCCCCEECCCC
PYGGGPGMVMIPKPLEAAIGAAKAAQAAQGIAATRVVMMSPQGAPLTHERVMRMVDEPGV
CCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCHHHHHHHHCCCCE
VLLCGRYEAIDQRLLDRCVDEEISLGDFVLSGGELPAMALMDAVVRQLPGVLNDTQSAVQ
EEEECCHHHHHHHHHHHHHHCCCCCCHHEECCCCCHHHHHHHHHHHHCCCHHHHHHHHHH
DSFVDGLLDCPHYTRPEEYEGARVPDVLLGGHHAEIEKWRRQEALRNTWQKRPDLIARAR
HHHHHHHHCCCCCCCCCCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHH
SKQLLSRADEAWLASLAKAAKQASN
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA