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The map label for this gene is ytwF [H]

Identifier: 238026430

GI number: 238026430

Start: 829464

End: 829793

Strand: Reverse

Name: ytwF [H]

Synonym: bglu_1g07650

Alternate gene names: 238026430

Gene position: 829793-829464 (Counterclockwise)

Preceding gene: 238026431

Following gene: 238026429

Centisome position: 21.24

GC content: 69.7

Gene sequence:

>330_bases
ATGCCGATGCAGATCCTGACCGCCCCCGCGCTGGCCGCCTGGCTCGCCGATCCGTCGCGCCCCGCGCCCGTCGTGCTCGA
CGTGCGCGAACCATGGGAGGTCGCGACCGCGAGCCTCGCCGGCAGCGTGTCGATCCCGATGCAGCAGATCCCCGCGCGCA
GCGAGGAACTCGATGACGAGGCCGAGATCGTCTGCGTCTGCCATCACGGCATGCGCAGCGCGCAGGTCGCGATGTTCCTC
GAATCGCGCGGCTTCACGAAGCTCTACAACCTGCAAGGCGGCATCGACGCGTGGTCGCGCGACGTCGACCCTTCGGTGCC
GCGCTACTGA

Upstream 100 bases:

>100_bases
TCCGCGTCGCCGACGTGTTCGAAACCTACGTCGACCACCTCGTCAACGCGATGGAGCCGTCGCGCTTCCGCTTCTGATTC
CCGCTGACCCGCTCACGCCC

Downstream 100 bases:

>100_bases
TGCACCGGTGTCGCGCCGTGCCGCGACGCCCACGGTAGCCGATCCCGCGACGCCGCGGCCCTGACCGGCCGCGGCGTCGG
CGCTTTCGGGCCACGCGGTT

Product: Rhodanese domain-containing protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: NA

Number of amino acids: Translated: 109; Mature: 108

Protein sequence:

>109_residues
MPMQILTAPALAAWLADPSRPAPVVLDVREPWEVATASLAGSVSIPMQQIPARSEELDDEAEIVCVCHHGMRSAQVAMFL
ESRGFTKLYNLQGGIDAWSRDVDPSVPRY

Sequences:

>Translated_109_residues
MPMQILTAPALAAWLADPSRPAPVVLDVREPWEVATASLAGSVSIPMQQIPARSEELDDEAEIVCVCHHGMRSAQVAMFL
ESRGFTKLYNLQGGIDAWSRDVDPSVPRY
>Mature_108_residues
PMQILTAPALAAWLADPSRPAPVVLDVREPWEVATASLAGSVSIPMQQIPARSEELDDEAEIVCVCHHGMRSAQVAMFLE
SRGFTKLYNLQGGIDAWSRDVDPSVPRY

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 11934; Mature: 11803

Theoretical pI: Translated: 4.42; Mature: 4.42

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMQILTAPALAAWLADPSRPAPVVLDVREPWEVATASLAGSVSIPMQQIPARSEELDDE
CCCCHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHCCCCCCCCHHHCCCCCCCCCCC
AEIVCVCHHGMRSAQVAMFLESRGFTKLYNLQGGIDAWSRDVDPSVPRY
CCEEEEECCCCCCHHHHEEECCCCCEEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PMQILTAPALAAWLADPSRPAPVVLDVREPWEVATASLAGSVSIPMQQIPARSEELDDE
CCCHHHHHHHHHHHCCCCCCCCEEEECCCCCHHHHHHCCCCCCCCHHHCCCCCCCCCCC
AEIVCVCHHGMRSAQVAMFLESRGFTKLYNLQGGIDAWSRDVDPSVPRY
CCEEEEECCCCCCHHHHEEECCCCCEEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]