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The map label for this gene is rfbB [H]

Identifier: 238026391

GI number: 238026391

Start: 784379

End: 785440

Strand: Direct

Name: rfbB [H]

Synonym: bglu_1g07260

Alternate gene names: 238026391

Gene position: 784379-785440 (Clockwise)

Preceding gene: 238026389

Following gene: 238026392

Centisome position: 20.08

GC content: 65.54

Gene sequence:

>1062_bases
ATGATCTTGGTGACGGGCGGCGCCGGTTTTATCGGCGCCAATTTCGTGCTGGACTGGCTCGCGCAATCCGGCGAGGCAGT
GCTGAATGTCGACAAGCTCACGTATGCGGGCAATCTCGGCACGCTGAAATCTCTGCAGGGCAATCCGGCCCACGTCTTCG
CCCGCGTCGATATCTGTGATCGTGCGGCGATCGACGCGCTGCTGGCCGAGCACCGCCCGCGCGCGATCCTGCATTTCGCC
GCGGAAAGCCACGTGGATCGCTCGATCCACGGCCCGGCCGAGTTCGTGCAGACCAACGTGGTCGGTACCTTCACGCTGCT
CGAAGCGGCGCGCCAGTACTGGAACACGCTCGAGGCCGACGCGAAGGCCGCGTTCCGCTTCCTGCACGTCTCTACCGACG
AGGTGTTCGGCTCGCTGTCGGCGAGCGACCCGCAGTTCTCCGAAACCACGCCGTATGCGCCGAACAGCCCCTATTCGGCC
ACCAAGGCAGGTTCGGACCATCTGGTGCGCGCCTATTTCCACACCTACGGGCTGCCGGTGCTGACCACCAACTGCTCGAA
CAACTACGGCCCCTACCAGTTCCCCGAAAAGCTGATTCCGCTGATGATCGCCAACGCGCTGGCCGGCAAGCCGCTGCCCG
TGTACGGCGACGGACAGAACGTGCGCGACTGGCTCTACGTGGGCGACCACTGCTCGGCGATCCGCGAGGTGCTCGCGCGC
GGCGTGCCCGGCGAAACTTACAACGTGGGCGGCTGGAACGAAAAGAAGAACCTCGACGTGGTCCACGTGCTGTGCGACCT
GCTCGACACGCTGCGCCCGAAGGCGGCCGGCTCGTACCGCGAGCAGGTGACGTTCGTCACCGATCGCCCCGGCCACGACC
GCCGCTACGCGATCGACGCGCGCAAGCTCGAGCGAGAGCTGGGCTGGAAGCCGGCCGAGACTTTCGAGACCGGCATGGAA
AAGACGGTGCGCTGGTATCTCGATAACCAGCCGTGGGCCGCCGAGGTTGCCTCGGGCGAATATCGTAACTGGGTCGAGAT
GAACTACGCGCGGCGCGCGTGA

Upstream 100 bases:

>100_bases
CGAACCTTTGCGATTCACTGACCCACATTGCGGGAGTGTCGTGGCGCCAGCCGACGACGGGGGCGTCCAGCCGAATCGTA
TTTCGATAAAAAGGGATCCA

Downstream 100 bases:

>100_bases
CGGAGGCGAGATGGCACGCAAGGGCATCATTCTGGCCGGCGGTTCCGGCACCCGTCTGTACCCGATCACGCATGCGGTGT
CGAAGCAGCTGCTGCCGGTG

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 353; Mature: 353

Protein sequence:

>353_residues
MILVTGGAGFIGANFVLDWLAQSGEAVLNVDKLTYAGNLGTLKSLQGNPAHVFARVDICDRAAIDALLAEHRPRAILHFA
AESHVDRSIHGPAEFVQTNVVGTFTLLEAARQYWNTLEADAKAAFRFLHVSTDEVFGSLSASDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYFHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
GVPGETYNVGGWNEKKNLDVVHVLCDLLDTLRPKAAGSYREQVTFVTDRPGHDRRYAIDARKLERELGWKPAETFETGME
KTVRWYLDNQPWAAEVASGEYRNWVEMNYARRA

Sequences:

>Translated_353_residues
MILVTGGAGFIGANFVLDWLAQSGEAVLNVDKLTYAGNLGTLKSLQGNPAHVFARVDICDRAAIDALLAEHRPRAILHFA
AESHVDRSIHGPAEFVQTNVVGTFTLLEAARQYWNTLEADAKAAFRFLHVSTDEVFGSLSASDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYFHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
GVPGETYNVGGWNEKKNLDVVHVLCDLLDTLRPKAAGSYREQVTFVTDRPGHDRRYAIDARKLERELGWKPAETFETGME
KTVRWYLDNQPWAAEVASGEYRNWVEMNYARRA
>Mature_353_residues
MILVTGGAGFIGANFVLDWLAQSGEAVLNVDKLTYAGNLGTLKSLQGNPAHVFARVDICDRAAIDALLAEHRPRAILHFA
AESHVDRSIHGPAEFVQTNVVGTFTLLEAARQYWNTLEADAKAAFRFLHVSTDEVFGSLSASDPQFSETTPYAPNSPYSA
TKAGSDHLVRAYFHTYGLPVLTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
GVPGETYNVGGWNEKKNLDVVHVLCDLLDTLRPKAAGSYREQVTFVTDRPGHDRRYAIDARKLERELGWKPAETFETGME
KTVRWYLDNQPWAAEVASGEYRNWVEMNYARRA

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=330, Percent_Identity=35.7575757575758, Blast_Score=216, Evalue=2e-56,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=24.2424242424242, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI4504031, Length=343, Percent_Identity=25.0728862973761, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI56237023, Length=361, Percent_Identity=25.7617728531856, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI56118217, Length=361, Percent_Identity=25.7617728531856, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI189083684, Length=361, Percent_Identity=25.7617728531856, Blast_Score=77, Evalue=3e-14,
Organism=Escherichia coli, GI1788353, Length=359, Percent_Identity=60.1671309192201, Blast_Score=457, Evalue=1e-130,
Organism=Escherichia coli, GI48994969, Length=344, Percent_Identity=62.2093023255814, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI1786974, Length=355, Percent_Identity=27.3239436619718, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788366, Length=353, Percent_Identity=25.4957507082153, Blast_Score=73, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17568069, Length=334, Percent_Identity=34.7305389221557, Blast_Score=201, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI115532424, Length=329, Percent_Identity=32.5227963525836, Blast_Score=174, Evalue=8e-44,
Organism=Caenorhabditis elegans, GI71982035, Length=356, Percent_Identity=28.0898876404494, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI71982038, Length=358, Percent_Identity=27.9329608938547, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17539532, Length=331, Percent_Identity=23.2628398791541, Blast_Score=85, Evalue=7e-17,
Organism=Caenorhabditis elegans, GI17507723, Length=336, Percent_Identity=25, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI133901786, Length=333, Percent_Identity=23.7237237237237, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539422, Length=333, Percent_Identity=23.7237237237237, Blast_Score=71, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133901790, Length=333, Percent_Identity=23.7237237237237, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI133901788, Length=333, Percent_Identity=23.7237237237237, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17539424, Length=333, Percent_Identity=23.7237237237237, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319493, Length=387, Percent_Identity=25.5813953488372, Blast_Score=90, Evalue=5e-19,
Organism=Drosophila melanogaster, GI19923002, Length=368, Percent_Identity=29.0760869565217, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI21356223, Length=331, Percent_Identity=24.773413897281, Blast_Score=88, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24158427, Length=344, Percent_Identity=25, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 39180; Mature: 39180

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILVTGGAGFIGANFVLDWLAQSGEAVLNVDKLTYAGNLGTLKSLQGNPAHVFARVDICD
CEEEECCCCHHHHHHHHHHHHCCCCEEEEEEHEEECCCCCCHHHCCCCCEEEEEEECHHH
RAAIDALLAEHRPRAILHFAAESHVDRSIHGPAEFVQTNVVGTFTLLEAARQYWNTLEAD
HHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AKAAFRFLHVSTDEVFGSLSASDPQFSETTPYAPNSPYSATKAGSDHLVRAYFHTYGLPV
HHHHHHEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
LTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEECHHHHHHHHHHHC
GVPGETYNVGGWNEKKNLDVVHVLCDLLDTLRPKAAGSYREQVTFVTDRPGHDRRYAIDA
CCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHEEEEEECCCCCCCEEEHHH
RKLERELGWKPAETFETGMEKTVRWYLDNQPWAAEVASGEYRNWVEMNYARRA
HHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCEEHHCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MILVTGGAGFIGANFVLDWLAQSGEAVLNVDKLTYAGNLGTLKSLQGNPAHVFARVDICD
CEEEECCCCHHHHHHHHHHHHCCCCEEEEEEHEEECCCCCCHHHCCCCCEEEEEEECHHH
RAAIDALLAEHRPRAILHFAAESHVDRSIHGPAEFVQTNVVGTFTLLEAARQYWNTLEAD
HHHHHHHHHCCCCCEEEEEHHHHHCCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHH
AKAAFRFLHVSTDEVFGSLSASDPQFSETTPYAPNSPYSATKAGSDHLVRAYFHTYGLPV
HHHHHHEEEEEHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCE
LTTNCSNNYGPYQFPEKLIPLMIANALAGKPLPVYGDGQNVRDWLYVGDHCSAIREVLAR
EEECCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCCHHEEECHHHHHHHHHHHC
GVPGETYNVGGWNEKKNLDVVHVLCDLLDTLRPKAAGSYREQVTFVTDRPGHDRRYAIDA
CCCCCEEECCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHEEEEEECCCCCCCEEEHHH
RKLERELGWKPAETFETGMEKTVRWYLDNQPWAAEVASGEYRNWVEMNYARRA
HHHHHHHCCCCHHHHHHHHHHHHHEEECCCCCEEHHCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8253667 [H]