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The map label for this gene is rlmL [H]

Identifier: 238026262

GI number: 238026262

Start: 624467

End: 625774

Strand: Reverse

Name: rlmL [H]

Synonym: bglu_1g05950

Alternate gene names: 238026262

Gene position: 625774-624467 (Counterclockwise)

Preceding gene: 238026263

Following gene: 238026261

Centisome position: 16.02

GC content: 72.4

Gene sequence:

>1308_bases
ATGTCCTCCTCCCTATTCGATTTCTTCGCTCCCTGCCCGCGTGGCCTCGAGGCCGCGCTCGCGGCCGAACTGGCCGAGAT
CGGCGCGCGGCGCATCGCCGGCGCGCCGTTCGAGGCCGGCACCCAGGTGCCCGGCGGCGTCCATTTTCGCGGCGGCTGGA
TGGCCGGCATGGCCGCGAACCTGCATTCGCGCATCGCGAGCCGCGTGCTGCTGAAGATCGCCGATCGGCCGTACCGCAGC
GAGCAGGACGTCTACGCGCTCGCGCTCGAACAGCAGTGGGAGCGCTGGTTCGCCGCCACCCAGACGCTGCGGGTGGACGT
CACCGCGATCAAGTCGCCGCTCAAGAGCCTCGAGTTCGCGACGCTGCGCGTGAAGGACGCGATCTGCGACCGGCTGCGCG
AGAAGACCGGCGCACGCCCGAGCATCGACACCGCCCAGCCCGACGTGCGCGTGTTCGCGTTCCTGACCGCGACGCACTGC
ACGCTCTACCTCGACACCTCCGGCGAGCCGCTGTTCAAGCGCGGCTGGCGGCTCGACAAGGGCGCGGCGCCGCTGCGCGA
GAACCTCGCGGCCGGCATCCTGCGCGTGGCGGGCTGGACCCCGGCCGTGCCGCTCTACGATCCGATGTGCGGCAGCGGCA
CGTTCCTCGCCGAGGCCGCCCAGATCGCGCTCGACGTGGCGCCGGGCGTGGAGCGCCGCTTCGGTTTCGAGAAGCTCAAG
CAGTACGACATCACCGCGTGGCAGACGCTGAAGGTGGCCGCGATGGACGCCAAGCGCGCCGCGCGCGGCAGGCACGCCGC
GCTGCCGGTGTACGGCAGCGACATCTCCGGCGACATGCTCGACAAGGCGCGCGCGAACCTCGAACGCGCGGGCGTGCCGG
CTATTTCGCTCAAGCAGGTCGACGCGCGCTTCATGGCACCGCCCACCGACACGCCGGGGCTGATCGTGGCAAACCCGCCG
TATGGCGAGCGCATCGAGGTGCGCGGGCGCGGCCCGCGCGGCGACGCACGCGAGACCGGCCGCAATCGCGGCAACGACGA
CGCGTTCCGCCGCACCCACACCGACGCGCCCGACGGCGAATTCTTCATCGCGCTCGGCAACGCGCTGAAGCAGCGCTTCG
CGGGCTGGCAGGCGTTCCTGCTGAGCTCGGACCGCTCGCTGCCGGGCCAGCTGCGCCTGCGCGAATCGGCGAAGACCCCG
CTCTTCAACGGCGCACTGGAATGCCGGCTGTTCCGCTTCGACGTGGTGGCGGGCAGCGTGCGGCAGCGCCCCGCGCAGGG
CGCCGAGGCGGCCGGCGGCGACGCCTAG

Upstream 100 bases:

>100_bases
GCATCGGCATGCCGGCGGCCCGGGCCGCGCGGGCGCGCGCCGGGCCGGCGGCGCCGCCCGCGTACAATGACGGCTTTTGC
ACGCCTCGCCACCCCGCCCC

Downstream 100 bases:

>100_bases
GCGCCGCCGGCGCGCGCCGCCTCGGCGCGCCATCGCAGCAGACCCGAGGGCCGGCCTTGACCGGCCCTTGGTTCGCCCTT
CTCGGCTCGCGCCCGCCCCG

Product: putative N6-adenine-specific DNA methylase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 435; Mature: 434

Protein sequence:

>435_residues
MSSSLFDFFAPCPRGLEAALAAELAEIGARRIAGAPFEAGTQVPGGVHFRGGWMAGMAANLHSRIASRVLLKIADRPYRS
EQDVYALALEQQWERWFAATQTLRVDVTAIKSPLKSLEFATLRVKDAICDRLREKTGARPSIDTAQPDVRVFAFLTATHC
TLYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRVAGWTPAVPLYDPMCGSGTFLAEAAQIALDVAPGVERRFGFEKLK
QYDITAWQTLKVAAMDAKRAARGRHAALPVYGSDISGDMLDKARANLERAGVPAISLKQVDARFMAPPTDTPGLIVANPP
YGERIEVRGRGPRGDARETGRNRGNDDAFRRTHTDAPDGEFFIALGNALKQRFAGWQAFLLSSDRSLPGQLRLRESAKTP
LFNGALECRLFRFDVVAGSVRQRPAQGAEAAGGDA

Sequences:

>Translated_435_residues
MSSSLFDFFAPCPRGLEAALAAELAEIGARRIAGAPFEAGTQVPGGVHFRGGWMAGMAANLHSRIASRVLLKIADRPYRS
EQDVYALALEQQWERWFAATQTLRVDVTAIKSPLKSLEFATLRVKDAICDRLREKTGARPSIDTAQPDVRVFAFLTATHC
TLYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRVAGWTPAVPLYDPMCGSGTFLAEAAQIALDVAPGVERRFGFEKLK
QYDITAWQTLKVAAMDAKRAARGRHAALPVYGSDISGDMLDKARANLERAGVPAISLKQVDARFMAPPTDTPGLIVANPP
YGERIEVRGRGPRGDARETGRNRGNDDAFRRTHTDAPDGEFFIALGNALKQRFAGWQAFLLSSDRSLPGQLRLRESAKTP
LFNGALECRLFRFDVVAGSVRQRPAQGAEAAGGDA
>Mature_434_residues
SSSLFDFFAPCPRGLEAALAAELAEIGARRIAGAPFEAGTQVPGGVHFRGGWMAGMAANLHSRIASRVLLKIADRPYRSE
QDVYALALEQQWERWFAATQTLRVDVTAIKSPLKSLEFATLRVKDAICDRLREKTGARPSIDTAQPDVRVFAFLTATHCT
LYLDTSGEPLFKRGWRLDKGAAPLRENLAAGILRVAGWTPAVPLYDPMCGSGTFLAEAAQIALDVAPGVERRFGFEKLKQ
YDITAWQTLKVAAMDAKRAARGRHAALPVYGSDISGDMLDKARANLERAGVPAISLKQVDARFMAPPTDTPGLIVANPPY
GERIEVRGRGPRGDARETGRNRGNDDAFRRTHTDAPDGEFFIALGNALKQRFAGWQAFLLSSDRSLPGQLRLRESAKTPL
FNGALECRLFRFDVVAGSVRQRPAQGAEAAGGDA

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=391, Percent_Identity=32.7365728900256, Blast_Score=202, Evalue=5e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 47320; Mature: 47189

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE ; PS00134 TRYPSIN_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSLFDFFAPCPRGLEAALAAELAEIGARRIAGAPFEAGTQVPGGVHFRGGWMAGMAAN
CCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHH
LHSRIASRVLLKIADRPYRSEQDVYALALEQQWERWFAATQTLRVDVTAIKSPLKSLEFA
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHH
TLRVKDAICDRLREKTGARPSIDTAQPDVRVFAFLTATHCTLYLDTSGEPLFKRGWRLDK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEEEEECCCCHHHHCCCCCCC
GAAPLRENLAAGILRVAGWTPAVPLYDPMCGSGTFLAEAAQIALDVAPGVERRFGFEKLK
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCHHHHHCHHHHH
QYDITAWQTLKVAAMDAKRAARGRHAALPVYGSDISGDMLDKARANLERAGVPAISLKQV
HHCCCHHHEEEEEHHHHHHHHCCCCEECEEECCCCCCHHHHHHHHHHHHCCCCEEEHHHH
DARFMAPPTDTPGLIVANPPYGERIEVRGRGPRGDARETGRNRGNDDAFRRTHTDAPDGE
CHHEECCCCCCCCEEEECCCCCCEEEEECCCCCCCHHHHCCCCCCCHHHHHCCCCCCCCC
FFIALGNALKQRFAGWQAFLLSSDRSLPGQLRLRESAKTPLFNGALECRLFRFDVVAGSV
EEEEHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHCCCCCCCCCEEEEEEEEEHHHCHH
RQRPAQGAEAAGGDA
HHCCCCCCCCCCCCC
>Mature Secondary Structure 
SSSLFDFFAPCPRGLEAALAAELAEIGARRIAGAPFEAGTQVPGGVHFRGGWMAGMAAN
CCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEECCCHHHHHHHH
LHSRIASRVLLKIADRPYRSEQDVYALALEQQWERWFAATQTLRVDVTAIKSPLKSLEFA
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHH
TLRVKDAICDRLREKTGARPSIDTAQPDVRVFAFLTATHCTLYLDTSGEPLFKRGWRLDK
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEEEEEEEECCCCHHHHCCCCCCC
GAAPLRENLAAGILRVAGWTPAVPLYDPMCGSGTFLAEAAQIALDVAPGVERRFGFEKLK
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCHHHHHCHHHHH
QYDITAWQTLKVAAMDAKRAARGRHAALPVYGSDISGDMLDKARANLERAGVPAISLKQV
HHCCCHHHEEEEEHHHHHHHHCCCCEECEEECCCCCCHHHHHHHHHHHHCCCCEEEHHHH
DARFMAPPTDTPGLIVANPPYGERIEVRGRGPRGDARETGRNRGNDDAFRRTHTDAPDGE
CHHEECCCCCCCCEEEECCCCCCEEEEECCCCCCCHHHHCCCCCCCHHHHHCCCCCCCCC
FFIALGNALKQRFAGWQAFLLSSDRSLPGQLRLRESAKTPLFNGALECRLFRFDVVAGSV
EEEEHHHHHHHHHCCCEEEEEECCCCCCCCEEEHHHCCCCCCCCCEEEEEEEEEHHHCHH
RQRPAQGAEAAGGDA
HHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA