| Definition | Burkholderia glumae BGR1 chromosome chromosome 1, complete sequence. |
|---|---|
| Accession | NC_012724 |
| Length | 3,906,507 |
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The map label for this gene is purB [H]
Identifier: 238026225
GI number: 238026225
Start: 587098
End: 588537
Strand: Reverse
Name: purB [H]
Synonym: bglu_1g05560
Alternate gene names: 238026225
Gene position: 588537-587098 (Counterclockwise)
Preceding gene: 238026232
Following gene: 238026220
Centisome position: 15.07
GC content: 67.01
Gene sequence:
>1440_bases ATGGTAAAATTGCGGGTTGACCCTCTCCACGCCCGAACCGGCCGCCCCATCATGTCCGACACCCGCCCCGACACCCTTTT CGCCCTCACCGCGCTCTCGCCGCTCGACGGCCGCTACGCGAGCAAGACCGAAGCGCTGCGCGAGTGGCTCTCCGAGGCCG CGTTCATGCGCCACCGCGTGACGGTCGAGGCTCATTGGCTGATCGCGCTGTCGCGCGCCGGCTTCGCCGAAGTGCCGCGC TTCTCGGCGGCCTCCGAGCAGTTCCTGCTGCAGCTGGTCGAGCGCTTCACCGCGCACGACGCGGCACGCATCAAGGACAT CGAGCGCGTCACCAACCACGACGTGAAGGCGGTCGAATACTGGCTCAAGGAATCGGTGAAGGGCCAGCCGGAACTCGAGG CCGCGAGCGAATTCATCCACTTCGCCTGCACCTCGGAAGACATCAACAACACCTCGCACGGCATGATGCTGGCCGGTGCG CGCGACAAGGTGATCGTGCCGGCGCTGCGCAGCGTGCAGCAGCGGCTCGTCGCGCTCGCGCACGCGCTGGCCGACCAGCC GATGCTGTCGCGCACGCACGGCCAGCCGGCCAGCCCGACCACGCTCGGCAAGGAAATCGCCAACGTCGCGGCGCGCCTCG CGCGCGCGATCGAGCGCATCGAGAAGGTCGAGCTGCTGGGCAAGATGAACGGCGCGGTCGGCAACTTCAACGCGCACCTG TCGGCGTATCCGGAATTCGACTGGGAGGCGTTCTCCAAGGACGTGGTCGAGAACCGCCTGAAGCTCGCCTTCAACCCGTA CACCATCCAGATCGAGCCGCACGACTACATGGCCGAGCTGTTCGACGCGGTGGCGCGCGCCAACACGATCCTGCTCGACC TCGACCGCGACGTCTGGGGCTACATCGCGCTCGGCTACTTCAAGCAGAAGACCAAGGCCGGCGAGATCGGCTCGTCGACC ATGCCGCACAAGGTCAACCCGATCGATTTCGAGAACTCGGAAGGCAACCTCGGCCTGGCCAACGCCACGCTGCGCCATCT CTCCGAGAAGCTGCCGGTGTCGCGCTGGCAGCGCGACCTGACCGACTCCACGGTGCTGCGCAACATCGGCGTGGCGTTCG GCTACTCGCTGCTCGCCTACGAATCGCTCACGCGCGGTCTCGACAAGCTCGAGGTGAACCCCGCGCGCCTGAACGAGGAC CTCGACAACTGCTGGGAAGTGCTGGCCGAGCCGGTGCAGACCGTGATGCGCCGCTACGGCATCGAGAACCCCTACGAGCA GCTGAAGGAACTGACGCGCGGCAAGGGCATCACGCGCGAGGCGCTGCAGCAGTTCGTGGGCGGCCTGGCGATCCCGCAGG ACGCGAAGGACCTGCTGCTGGCGATGACGCCGGCCTCGTACATCGGCAAGGCGGTCGAGCTCGCGAAGCGGATCGCGTAA
Upstream 100 bases:
>100_bases CCCGCATTGTAGCCGGGCGGCGGCGGCGCAGGCGAAGGCGCCCGCGCGGGCCGCCCCCCGGCGCCCTGCCCGCACCGCGT GCCGGAGCCGGCCGCCGCCG
Downstream 100 bases:
>100_bases CGCGCGAGGCATCACGGCGTGCCGCCTCGCGGCGGCACGCAAAAACCAAGGCGCCCGGGGCATTCGCCGCCGGGCGCCTT GTTTTGTCTGGTCGCGCGGG
Product: adenylosuccinate lyase
Products: NA
Alternate protein names: ASL; Adenylosuccinase; ASase [H]
Number of amino acids: Translated: 479; Mature: 479
Protein sequence:
>479_residues MVKLRVDPLHARTGRPIMSDTRPDTLFALTALSPLDGRYASKTEALREWLSEAAFMRHRVTVEAHWLIALSRAGFAEVPR FSAASEQFLLQLVERFTAHDAARIKDIERVTNHDVKAVEYWLKESVKGQPELEAASEFIHFACTSEDINNTSHGMMLAGA RDKVIVPALRSVQQRLVALAHALADQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVELLGKMNGAVGNFNAHL SAYPEFDWEAFSKDVVENRLKLAFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYIALGYFKQKTKAGEIGSST MPHKVNPIDFENSEGNLGLANATLRHLSEKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYESLTRGLDKLEVNPARLNED LDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGITREALQQFVGGLAIPQDAKDLLLAMTPASYIGKAVELAKRIA
Sequences:
>Translated_479_residues MVKLRVDPLHARTGRPIMSDTRPDTLFALTALSPLDGRYASKTEALREWLSEAAFMRHRVTVEAHWLIALSRAGFAEVPR FSAASEQFLLQLVERFTAHDAARIKDIERVTNHDVKAVEYWLKESVKGQPELEAASEFIHFACTSEDINNTSHGMMLAGA RDKVIVPALRSVQQRLVALAHALADQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVELLGKMNGAVGNFNAHL SAYPEFDWEAFSKDVVENRLKLAFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYIALGYFKQKTKAGEIGSST MPHKVNPIDFENSEGNLGLANATLRHLSEKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYESLTRGLDKLEVNPARLNED LDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGITREALQQFVGGLAIPQDAKDLLLAMTPASYIGKAVELAKRIA >Mature_479_residues MVKLRVDPLHARTGRPIMSDTRPDTLFALTALSPLDGRYASKTEALREWLSEAAFMRHRVTVEAHWLIALSRAGFAEVPR FSAASEQFLLQLVERFTAHDAARIKDIERVTNHDVKAVEYWLKESVKGQPELEAASEFIHFACTSEDINNTSHGMMLAGA RDKVIVPALRSVQQRLVALAHALADQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVELLGKMNGAVGNFNAHL SAYPEFDWEAFSKDVVENRLKLAFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWGYIALGYFKQKTKAGEIGSST MPHKVNPIDFENSEGNLGLANATLRHLSEKLPVSRWQRDLTDSTVLRNIGVAFGYSLLAYESLTRGLDKLEVNPARLNED LDNCWEVLAEPVQTVMRRYGIENPYEQLKELTRGKGITREALQQFVGGLAIPQDAKDLLLAMTPASYIGKAVELAKRIA
Specific function: De novo purine biosynthesis; eighth step. [C]
COG id: COG0015
COG function: function code F; Adenylosuccinate lyase
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]
Homologues:
Organism=Escherichia coli, GI1787376, Length=454, Percent_Identity=62.9955947136564, Blast_Score=591, Evalue=1e-170,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR013539 - InterPro: IPR000362 - InterPro: IPR020557 - InterPro: IPR008948 - InterPro: IPR022761 - InterPro: IPR004769 [H]
Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]
EC number: =4.3.2.2 [H]
Molecular weight: Translated: 53467; Mature: 53467
Theoretical pI: Translated: 6.72; Mature: 6.72
Prosite motif: PS00163 FUMARATE_LYASES
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.4 %Cys (Translated Protein) 2.3 %Met (Translated Protein) 2.7 %Cys+Met (Translated Protein) 0.4 %Cys (Mature Protein) 2.3 %Met (Mature Protein) 2.7 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MVKLRVDPLHARTGRPIMSDTRPDTLFALTALSPLDGRYASKTEALREWLSEAAFMRHRV CEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH TVEAHWLIALSRAGFAEVPRFSAASEQFLLQLVERFTAHDAARIKDIERVTNHDVKAVEY EEEEHEEEEECCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH WLKESVKGQPELEAASEFIHFACTSEDINNTSHGMMLAGARDKVIVPALRSVQQRLVALA HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH HALADQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVELLGKMNGAVGNFNAHL HHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE SAYPEFDWEAFSKDVVENRLKLAFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWG ECCCCCCHHHHHHHHHHHHHHEEECCEEEEECCHHHHHHHHHHHHHCCEEEEECCCCHHH YIALGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNLGLANATLRHLSEKLPVSRWQRDL HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH TDSTVLRNIGVAFGYSLLAYESLTRGLDKLEVNPARLNEDLDNCWEVLAEPVQTVMRRYG HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC IENPYEQLKELTRGKGITREALQQFVGGLAIPQDAKDLLLAMTPASYIGKAVELAKRIA CCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHEEECCHHHHHHHHHHHHHCC >Mature Secondary Structure MVKLRVDPLHARTGRPIMSDTRPDTLFALTALSPLDGRYASKTEALREWLSEAAFMRHRV CEEEEECCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHH TVEAHWLIALSRAGFAEVPRFSAASEQFLLQLVERFTAHDAARIKDIERVTNHDVKAVEY EEEEHEEEEECCCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH WLKESVKGQPELEAASEFIHFACTSEDINNTSHGMMLAGARDKVIVPALRSVQQRLVALA HHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH HALADQPMLSRTHGQPASPTTLGKEIANVAARLARAIERIEKVELLGKMNGAVGNFNAHL HHHHCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCE SAYPEFDWEAFSKDVVENRLKLAFNPYTIQIEPHDYMAELFDAVARANTILLDLDRDVWG ECCCCCCHHHHHHHHHHHHHHEEECCEEEEECCHHHHHHHHHHHHHCCEEEEECCCCHHH YIALGYFKQKTKAGEIGSSTMPHKVNPIDFENSEGNLGLANATLRHLSEKLPVSRWQRDL HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHH TDSTVLRNIGVAFGYSLLAYESLTRGLDKLEVNPARLNEDLDNCWEVLAEPVQTVMRRYG HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC IENPYEQLKELTRGKGITREALQQFVGGLAIPQDAKDLLLAMTPASYIGKAVELAKRIA CCCHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHEEECCHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10984043 [H]