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The map label for this gene is dctA2 [H]

Identifier: 238026207

GI number: 238026207

Start: 567384

End: 568706

Strand: Direct

Name: dctA2 [H]

Synonym: bglu_1g05380

Alternate gene names: 238026207

Gene position: 567384-568706 (Clockwise)

Preceding gene: 238026205

Following gene: 238026208

Centisome position: 14.52

GC content: 68.18

Gene sequence:

>1323_bases
ATGGCGAAGTTTCTGAACTCGTTGTTCGGGCGCGTGATGATCGCGCTCGTGATCGGGATTGCGCTCGGTGCGGCGTTTCC
GCATGCCGCGCAATCGCTGCGGCCGCTTGGCGACGGCTTCCTGAAGCTGATCAAGATGGTGATCGGGCCGATCGTGTTCT
GCGTGGTGGTGAGCGGCATCGCGAACGCCGGCGATCTGCGCAAGGTCGGGCGCGTCGGCCTGCGGGCGGTGATCTACTTC
GAGCTGATGACCACGTTCGCGCTCGCGATCGGCCTCGTGCTCGCGGAGCTCACGCGCCCCGGCGCCGGCATGAACGTGGA
TGTGGGCGCGCTCGATCCGGCCTCGCTCGCCGACTACGCGAAGCATGCGCAGAGCCTGAAGGACACCGCCGGCTATCTGC
TGAAGATCATTCCCGACACCGCGTTCGACGCGTTCGCCAAGGGCGACATCCTGCAGATCCTCGTGTTCGCGGTGCCGTTC
GGCGTGGCGCTGTCGATGCTCGGCGAGCGCGCGCGCGCGCTCAGCGGCCTCATCGACGAGTTCGCGCAGGTCTGCTTCCG
CGTGATGGGCTTCATCGTCCGGCTCGCGCCGCTCGGCGTGCTCGGCGCGATCGCGTACACCACCGGCAAGTACGGCGTGG
CCTCGCTCGAGCAGATGGGCCTGCTGGTGGCGGTGTTCTATCTGGCCTGCTTGCTGTTCGTGGTGGTGGTGCTCGGCGCG
GTGATGCGGCTCGCCGGCTTCAGCGTGTTCAAGCTGATCCGCTATCTGCGCGAGGAACTGATGATCGTGCTCGGCACCGC
CTCGTCGGACGCCGTGCTGCCGCAGGTGATGCGCAAGCTCGAATGGATGGGCGTCAAGGATTCGACGGTCGGGCTGGTGA
TCCCGACCGGCTACTCGTTCAACCTCGACGGCTTCTCGATCTACCTGACGCTCGCCGTGCTGTTCATCGCGCAGGCCACC
AACACGCCGCTGTCGGCGCACGATCTGATCGTGGTGCTGCTGGTCTCGCTGGTCACCTCGAAGGGCGCGCACGGCATTCC
CGGCTCGGCGATCGTGATCCTCGCCGCGACGCTCTCGGCGATCCCGGCGCTGCCGGTGCTCGGCCTCGTGCTGATCCTGC
CGGTGGACTGGTTCGTCGGCATCGCGCGCGCCGTGACGAACCTGCTCGGCAACTGCGTCGCGACGGTGGTGGTGGCGGTG
TGGGAGCACGACATCGACGCGGCCCGCGCGCGCCGCGTGCTGAACCGCGAGCTGCGCTACGTGCCGTCCGGCGACGCGCC
GGCCGGCGAATTGGCCCCGGAAGAGCCGGCCCCGGCGCTCTGA

Upstream 100 bases:

>100_bases
ATATCGGCCTCCTACACTCCGGCATCCGCTTACTGGTATGACCAGTCGGAACAGTGAGCATCTCTCGGCAAACCGCGGTA
CCCGCAGTACGGAGGATCGG

Downstream 100 bases:

>100_bases
GGCCAGCGCCCGCGACCTGATCCCGGTCCCGCGCGCGGCAGGCCGCGCGTGCGGATCGGCCGACTTTCGATTACCGCTTA
ACGGCAGGAGCCCGACCATG

Product: C4-dicarboxylate transporter DctA

Products: orotate [Cytoplasm]; fumarate [Cytoplasm]; malate [Cytoplasm]; Na (I) [Cytoplasm]; succinate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 440; Mature: 439

Protein sequence:

>440_residues
MAKFLNSLFGRVMIALVIGIALGAAFPHAAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGIANAGDLRKVGRVGLRAVIYF
ELMTTFALAIGLVLAELTRPGAGMNVDVGALDPASLADYAKHAQSLKDTAGYLLKIIPDTAFDAFAKGDILQILVFAVPF
GVALSMLGERARALSGLIDEFAQVCFRVMGFIVRLAPLGVLGAIAYTTGKYGVASLEQMGLLVAVFYLACLLFVVVVLGA
VMRLAGFSVFKLIRYLREELMIVLGTASSDAVLPQVMRKLEWMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVLFIAQAT
NTPLSAHDLIVVLLVSLVTSKGAHGIPGSAIVILAATLSAIPALPVLGLVLILPVDWFVGIARAVTNLLGNCVATVVVAV
WEHDIDAARARRVLNRELRYVPSGDAPAGELAPEEPAPAL

Sequences:

>Translated_440_residues
MAKFLNSLFGRVMIALVIGIALGAAFPHAAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGIANAGDLRKVGRVGLRAVIYF
ELMTTFALAIGLVLAELTRPGAGMNVDVGALDPASLADYAKHAQSLKDTAGYLLKIIPDTAFDAFAKGDILQILVFAVPF
GVALSMLGERARALSGLIDEFAQVCFRVMGFIVRLAPLGVLGAIAYTTGKYGVASLEQMGLLVAVFYLACLLFVVVVLGA
VMRLAGFSVFKLIRYLREELMIVLGTASSDAVLPQVMRKLEWMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVLFIAQAT
NTPLSAHDLIVVLLVSLVTSKGAHGIPGSAIVILAATLSAIPALPVLGLVLILPVDWFVGIARAVTNLLGNCVATVVVAV
WEHDIDAARARRVLNRELRYVPSGDAPAGELAPEEPAPAL
>Mature_439_residues
AKFLNSLFGRVMIALVIGIALGAAFPHAAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGIANAGDLRKVGRVGLRAVIYFE
LMTTFALAIGLVLAELTRPGAGMNVDVGALDPASLADYAKHAQSLKDTAGYLLKIIPDTAFDAFAKGDILQILVFAVPFG
VALSMLGERARALSGLIDEFAQVCFRVMGFIVRLAPLGVLGAIAYTTGKYGVASLEQMGLLVAVFYLACLLFVVVVLGAV
MRLAGFSVFKLIRYLREELMIVLGTASSDAVLPQVMRKLEWMGVKDSTVGLVIPTGYSFNLDGFSIYLTLAVLFIAQATN
TPLSAHDLIVVLLVSLVTSKGAHGIPGSAIVILAATLSAIPALPVLGLVLILPVDWFVGIARAVTNLLGNCVATVVVAVW
EHDIDAARARRVLNRELRYVPSGDAPAGELAPEEPAPAL

Specific function: Responsible for the transport of dicarboxylates such as succinate, fumarate, and malate from the periplasm across the membrane [H]

COG id: COG1301

COG function: function code C; Na+/H+-dicarboxylate symporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:dicarboxylate (SDF) symporter (TC 2.A.23) family [H]

Homologues:

Organism=Homo sapiens, GI169790839, Length=466, Percent_Identity=26.3948497854077, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI4827012, Length=297, Percent_Identity=29.96632996633, Blast_Score=115, Evalue=9e-26,
Organism=Homo sapiens, GI5032093, Length=400, Percent_Identity=29, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI21314632, Length=448, Percent_Identity=27.2321428571429, Blast_Score=109, Evalue=5e-24,
Organism=Homo sapiens, GI40254478, Length=447, Percent_Identity=23.9373601789709, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI223468566, Length=272, Percent_Identity=30.5147058823529, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI223468564, Length=272, Percent_Identity=30.5147058823529, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI66773030, Length=394, Percent_Identity=24.8730964467005, Blast_Score=97, Evalue=3e-20,
Organism=Homo sapiens, GI262359914, Length=391, Percent_Identity=25.3196930946292, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI301601644, Length=212, Percent_Identity=30.188679245283, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI194239697, Length=265, Percent_Identity=25.6603773584906, Blast_Score=80, Evalue=4e-15,
Organism=Escherichia coli, GI1789947, Length=413, Percent_Identity=51.089588377724, Blast_Score=427, Evalue=1e-121,
Organism=Escherichia coli, GI1790514, Length=427, Percent_Identity=38.8758782201405, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1789473, Length=335, Percent_Identity=25.3731343283582, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788024, Length=395, Percent_Identity=23.2911392405063, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17537407, Length=421, Percent_Identity=26.603325415677, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI71996953, Length=454, Percent_Identity=26.8722466960352, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI193206654, Length=433, Percent_Identity=25.8660508083141, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI193206505, Length=468, Percent_Identity=26.2820512820513, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71983099, Length=436, Percent_Identity=25.4587155963303, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71983106, Length=436, Percent_Identity=25.4587155963303, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17541374, Length=399, Percent_Identity=24.812030075188, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24583025, Length=402, Percent_Identity=23.6318407960199, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI17137668, Length=402, Percent_Identity=23.6318407960199, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI24583023, Length=402, Percent_Identity=23.6318407960199, Blast_Score=109, Evalue=3e-24,
Organism=Drosophila melanogaster, GI281360483, Length=463, Percent_Identity=25.2699784017279, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI17137666, Length=463, Percent_Identity=25.2699784017279, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI281360481, Length=463, Percent_Identity=25.2699784017279, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR018107 [H]

Pfam domain/function: PF00375 SDF [H]

EC number: NA

Molecular weight: Translated: 46423; Mature: 46292

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: PS00713 NA_DICARBOXYL_SYMP_1 ; PS00714 NA_DICARBOXYL_SYMP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AKFLNSLFGRVMIALVIGIALGAAFPHAAQSLRPLGDGFLKLIKMVIGPIVFCVVVSGI
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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CCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: orotate [Periplasm]; fumarate [Periplasm]; malate [Periplasm]; Na (I) [Periplasm]; succinate [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + orotate [Periplasm] = Na (I) [Cytoplasm] + orotate [Cytoplasm] Na (I) [Periplasm] + fumarate [Periplasm] = Na (I) [Cytoplasm] + fumarate [Cytoplasm] Na (I) [Periplasm] + malate [Periplasm] = Na (I) [Cytoplasm] + malate [Cytoplasm] Na

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]