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The map label for this gene is ddl [H]

Identifier: 238026134

GI number: 238026134

Start: 490869

End: 491810

Strand: Direct

Name: ddl [H]

Synonym: bglu_1g04640

Alternate gene names: 238026134

Gene position: 490869-491810 (Clockwise)

Preceding gene: 238026133

Following gene: 238026135

Centisome position: 12.57

GC content: 68.68

Gene sequence:

>942_bases
ATGAGCGGGATCGATCCGAAACGTTTCGGCAAGGTGGCGGTACTGTTCGGCGGCGTGTCCGCCGAGCGCGAGGTGTCGCT
GAATTCGGGCCGGCTGGTGCTGCAGGGTCTGCGCGAGGCGGGCATCGACGCGCATCTGTTCGATCCGTCCGAGCGGCCGC
TGTCGGCGCTGAAGGACGAGGGCTTCGTGCGCGCGTTCAACGCGCTGCACGGCGGTTACGGCGAGAACGGCCAGCTCCAG
GGCGCGCTCGATTTCTACGGGATCCGCTATACCGGCCCCGGCGTGCTCGGCTCGGCACTCGGCCTCGACAAGTTCCGCAC
CAAGCTGGTCTGGCAGCAGACCGGCATCCCGACGCCGCCGTTCGAGACCGTGATGCGCGGTGACGACCACGCCGCGCGCG
CGGCGGAGATCGTCGCGAAGCTGGGCCTGCCGCTGTTCGTGAAGCCGGCGAGCGAGGGCTCGAGCGTCGCCGTGATCAAG
GTGAAGGACGCCGCCGCGCTGCCGGCCGCGCTCGACGAGGCGGGCCGGCACGACCGGATCGTGATCGTCGAGAAGAGCAT
CGAAGGCGGCGGCGAGTACACGGCCTGCATCGCGGGCGACCTCGATCTGCCGGTGATCCGCATCGTGCCGGCGGGCGAGT
TCTACGACTATCACGCGAAGTACCTCGCCGACGACACGCAGTACCTGATCCCATGCGGGATCGAGGCGGCTAAGGAAACG
GAATTCAAGCGGCTCGCGCGCCGTGCGTTCGAGGTGCTCGGCTGCAGCGGCTGGGGCCGCGCCGATTTCATGCTCGACGC
GGCGGGCAACCCGTATTTCCTGGAAGTGAACACGGCGCCGGGGATGACCGACCACTCGCTGCCGCCGAAGGCCGCGCGCG
CGGTGGGCATCAGCTACTCGGAGCTGGTGGTGAAGGTGTTGTCGCTGACGCTGGACGACTGA

Upstream 100 bases:

>100_bases
CAGCGCGTGCGCGCGGGCGACGTGGTGATCACGATGGGCGCGGGGTCGATCGGCGGCGTGCCGGGCCGGATCGCGCAGCA
ACAGCAACGGAAGGAATGAC

Downstream 100 bases:

>100_bases
CCCGGATACGGAACCGACTCATGTGGAACAACGTTCGCCAGCTCAACCTCGCGGCCAGCGCGCTTTACGCGCTGCTGCTG
CTCGGTTGCACGGCGGCGGG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 313; Mature: 312

Protein sequence:

>313_residues
MSGIDPKRFGKVAVLFGGVSAEREVSLNSGRLVLQGLREAGIDAHLFDPSERPLSALKDEGFVRAFNALHGGYGENGQLQ
GALDFYGIRYTGPGVLGSALGLDKFRTKLVWQQTGIPTPPFETVMRGDDHAARAAEIVAKLGLPLFVKPASEGSSVAVIK
VKDAAALPAALDEAGRHDRIVIVEKSIEGGGEYTACIAGDLDLPVIRIVPAGEFYDYHAKYLADDTQYLIPCGIEAAKET
EFKRLARRAFEVLGCSGWGRADFMLDAAGNPYFLEVNTAPGMTDHSLPPKAARAVGISYSELVVKVLSLTLDD

Sequences:

>Translated_313_residues
MSGIDPKRFGKVAVLFGGVSAEREVSLNSGRLVLQGLREAGIDAHLFDPSERPLSALKDEGFVRAFNALHGGYGENGQLQ
GALDFYGIRYTGPGVLGSALGLDKFRTKLVWQQTGIPTPPFETVMRGDDHAARAAEIVAKLGLPLFVKPASEGSSVAVIK
VKDAAALPAALDEAGRHDRIVIVEKSIEGGGEYTACIAGDLDLPVIRIVPAGEFYDYHAKYLADDTQYLIPCGIEAAKET
EFKRLARRAFEVLGCSGWGRADFMLDAAGNPYFLEVNTAPGMTDHSLPPKAARAVGISYSELVVKVLSLTLDD
>Mature_312_residues
SGIDPKRFGKVAVLFGGVSAEREVSLNSGRLVLQGLREAGIDAHLFDPSERPLSALKDEGFVRAFNALHGGYGENGQLQG
ALDFYGIRYTGPGVLGSALGLDKFRTKLVWQQTGIPTPPFETVMRGDDHAARAAEIVAKLGLPLFVKPASEGSSVAVIKV
KDAAALPAALDEAGRHDRIVIVEKSIEGGGEYTACIAGDLDLPVIRIVPAGEFYDYHAKYLADDTQYLIPCGIEAAKETE
FKRLARRAFEVLGCSGWGRADFMLDAAGNPYFLEVNTAPGMTDHSLPPKAARAVGISYSELVVKVLSLTLDD

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786280, Length=303, Percent_Identity=53.1353135313531, Blast_Score=306, Evalue=8e-85,
Organism=Escherichia coli, GI1786579, Length=348, Percent_Identity=32.183908045977, Blast_Score=162, Evalue=4e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 33478; Mature: 33346

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS50975 ATP_GRASP ; PS00843 DALA_DALA_LIGASE_1 ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGIDPKRFGKVAVLFGGVSAEREVSLNSGRLVLQGLREAGIDAHLFDPSERPLSALKDE
CCCCCCHHHCCEEEEEECCCCCEEEECCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHCC
GFVRAFNALHGGYGENGQLQGALDFYGIRYTGPGVLGSALGLDKFRTKLVWQQTGIPTPP
CHHHHHHHHCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHCHHHHHHHHHHHCCCCCCCC
FETVMRGDDHAARAAEIVAKLGLPLFVKPASEGSSVAVIKVKDAAALPAALDEAGRHDRI
HHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCHHCCHHHHHCCCCCEE
VIVEKSIEGGGEYTACIAGDLDLPVIRIVPAGEFYDYHAKYLADDTQYLIPCGIEAAKET
EEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEECCCCHHHHH
EFKRLARRAFEVLGCSGWGRADFMLDAAGNPYFLEVNTAPGMTDHSLPPKAARAVGISYS
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCHH
ELVVKVLSLTLDD
HHHHHHHHHHCCC
>Mature Secondary Structure 
SGIDPKRFGKVAVLFGGVSAEREVSLNSGRLVLQGLREAGIDAHLFDPSERPLSALKDE
CCCCCHHHCCEEEEEECCCCCEEEECCCCHHHHHHHHHCCCEEEEECCCCCHHHHHHCC
GFVRAFNALHGGYGENGQLQGALDFYGIRYTGPGVLGSALGLDKFRTKLVWQQTGIPTPP
CHHHHHHHHCCCCCCCCEEEEEEEEEEEEEECCCHHHHHHCHHHHHHHHHHHCCCCCCCC
FETVMRGDDHAARAAEIVAKLGLPLFVKPASEGSSVAVIKVKDAAALPAALDEAGRHDRI
HHHHHCCCCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEECCCHHCCHHHHHCCCCCEE
VIVEKSIEGGGEYTACIAGDLDLPVIRIVPAGEFYDYHAKYLADDTQYLIPCGIEAAKET
EEEEECCCCCCCEEEEEECCCCCCEEEEEECCCCHHHHHHHHCCCCCEEEECCCCHHHHH
EFKRLARRAFEVLGCSGWGRADFMLDAAGNPYFLEVNTAPGMTDHSLPPKAARAVGISYS
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCCCCCCHHHHHHCCCHH
ELVVKVLSLTLDD
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA