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The map label for this gene is prfA [H]

Identifier: 238026092

GI number: 238026092

Start: 436982

End: 438064

Strand: Reverse

Name: prfA [H]

Synonym: bglu_1g04200

Alternate gene names: 238026092

Gene position: 438064-436982 (Counterclockwise)

Preceding gene: 238026093

Following gene: 238026091

Centisome position: 11.21

GC content: 68.24

Gene sequence:

>1083_bases
ATGAAAACGAGCATGCAAAGCAAGCTCGACCAGCTTTCCAGCCGGCTGGCCGAACTGAACGAACTGCTGAGCCGCGAAAA
CGTCACCGCCGATCTCGACCAATACCGCAAGCTCACGCGCGAACACGCCGAAATCGGCCCGGTGGTCGAGCAATACGCGC
AATGGCGCCTCGCGCGCGGCGACGAGGCCGCCGCGCAGGAGCTGCTGGCCGACGCCTCGATGCGCGAGTTCGCCGAGGAG
GAGATCCGCGAGGCCCGCGAGCGCATGGCGCGGCTCGAGGCCGAGCTGCAGACCATGCTGCTGCCGAAGGATCCGAACGA
CGACCGCAACATCTTCGTCGAGATCCGCGCGGGCACGGGCGGCGACGAGTCGGCGCTGTTCGCGGGCGACCTGCTGCGCA
TGTATCTGCGCTACGCGGAGCGCCAGCGCTGGCAGGTGGAGATGATGTCGGAGAGCGGCTCGGACCTCGGCGGCTACAAG
GAAGTGATCGTGCGCATCGCCGGCCACGGCGCCTATTCGCGGCTCAAGTTCGAATCGGGCGGCCATCGCGTGCAGCGCGT
GCCGGCCACCGAGACGCAGGGCCGGATCCACACCTCGGCCTGCACGGTGGCGGTGATGCCCGAGGCCGACGAGATCGCGG
AAGTCGAGATCAACCCGGCCGACCTGCGCATCGACACGTTCCGCGCCTCGGGCGCGGGCGGCCAGCACATCAACAAGACC
GACTCGGCGGTGCGCATCACCCACCTGCCGACCGGCATCGTGGTGGAGTGCCAGGACGACCGCTCGCAGCACAAGAACAA
GGATCGCGCGCTGAAGGTGCTGGCCGCGCGCATCAAGGACAAGCAGTACCACGAGCAGCACGCCCGGGAAGCGGCCACGC
GCAAGAGCCTGATCGGCTCGGGCGACCGCTCGGAGCGGATCCGCACCTACAACTTCCCGCAGGGCCGGATGACCGACCAC
CGTATCAACCTGACGCTGTACCGGCTCGAGGCGATCATGGACGGCGACCTCGACGAGCTGATCGCCTCGCTCGCGAGCGA
GCACCAGGCCGAGCTGCTCGCCTCGCTCGGCGAAGCGGACTGA

Upstream 100 bases:

>100_bases
GCCCGCGCGCCGACAACTAGCGCCGCTGCGCCTTCGGCCGCAGTGCCGGCCCTCCCCCTTTTTCCTTTCGATTCCTCGCC
CGGGAGCATCGCTCCAGATC

Downstream 100 bases:

>100_bases
CGCGGGCGGCGCCGATCGTCATGAACGCCTCGCCCGTCACCGCGGCCGGCCTGCTGCGCGCCAGCCCGCTCGACGCCGTC
GACGCGCGCATCCTGCTCGC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKTSMQSKLDQLSSRLAELNELLSRENVTADLDQYRKLTREHAEIGPVVEQYAQWRLARGDEAAAQELLADASMREFAEE
EIREARERMARLEAELQTMLLPKDPNDDRNIFVEIRAGTGGDESALFAGDLLRMYLRYAERQRWQVEMMSESGSDLGGYK
EVIVRIAGHGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIAEVEINPADLRIDTFRASGAGGQHINKT
DSAVRITHLPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAREAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYRLEAIMDGDLDELIASLASEHQAELLASLGEAD

Sequences:

>Translated_360_residues
MKTSMQSKLDQLSSRLAELNELLSRENVTADLDQYRKLTREHAEIGPVVEQYAQWRLARGDEAAAQELLADASMREFAEE
EIREARERMARLEAELQTMLLPKDPNDDRNIFVEIRAGTGGDESALFAGDLLRMYLRYAERQRWQVEMMSESGSDLGGYK
EVIVRIAGHGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIAEVEINPADLRIDTFRASGAGGQHINKT
DSAVRITHLPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAREAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYRLEAIMDGDLDELIASLASEHQAELLASLGEAD
>Mature_360_residues
MKTSMQSKLDQLSSRLAELNELLSRENVTADLDQYRKLTREHAEIGPVVEQYAQWRLARGDEAAAQELLADASMREFAEE
EIREARERMARLEAELQTMLLPKDPNDDRNIFVEIRAGTGGDESALFAGDLLRMYLRYAERQRWQVEMMSESGSDLGGYK
EVIVRIAGHGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIAEVEINPADLRIDTFRASGAGGQHINKT
DSAVRITHLPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAREAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYRLEAIMDGDLDELIASLASEHQAELLASLGEAD

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=284, Percent_Identity=50, Blast_Score=279, Evalue=3e-75,
Organism=Homo sapiens, GI34577120, Length=369, Percent_Identity=38.2113821138211, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI166795305, Length=189, Percent_Identity=48.1481481481481, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI1787462, Length=358, Percent_Identity=61.731843575419, Blast_Score=444, Evalue=1e-126,
Organism=Escherichia coli, GI2367172, Length=341, Percent_Identity=39.0029325513196, Blast_Score=223, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17542784, Length=331, Percent_Identity=33.8368580060423, Blast_Score=187, Evalue=7e-48,
Organism=Saccharomyces cerevisiae, GI6321295, Length=330, Percent_Identity=41.2121212121212, Blast_Score=248, Evalue=1e-66,
Organism=Drosophila melanogaster, GI19921226, Length=338, Percent_Identity=41.1242603550296, Blast_Score=226, Evalue=1e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40512; Mature: 40512

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSMQSKLDQLSSRLAELNELLSRENVTADLDQYRKLTREHAEIGPVVEQYAQWRLARG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DEAAAQELLADASMREFAEEEIREARERMARLEAELQTMLLPKDPNDDRNIFVEIRAGTG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCC
GDESALFAGDLLRMYLRYAERQRWQVEMMSESGSDLGGYKEVIVRIAGHGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHEEEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEADEIAEVEINPADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCCEEECEEEEEECCCCCCEEEEEECCHHEEEEEEECCCCCCCCCCCC
DSAVRITHLPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAREAATRKSLIGS
CCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQGRMTDHRINLTLYRLEAIMDGDLDELIASLASEHQAELLASLGEAD
CCCCCCCEEECCCCCCCCCCEEEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKTSMQSKLDQLSSRLAELNELLSRENVTADLDQYRKLTREHAEIGPVVEQYAQWRLARG
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DEAAAQELLADASMREFAEEEIREARERMARLEAELQTMLLPKDPNDDRNIFVEIRAGTG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCC
GDESALFAGDLLRMYLRYAERQRWQVEMMSESGSDLGGYKEVIVRIAGHGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHEEEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEADEIAEVEINPADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCCEEECEEEEEECCCCCCEEEEEECCHHEEEEEEECCCCCCCCCCCC
DSAVRITHLPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAREAATRKSLIGS
CCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQGRMTDHRINLTLYRLEAIMDGDLDELIASLASEHQAELLASLGEAD
CCCCCCCEEECCCCCCCCCCEEEEEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA