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The map label for this gene is hisH [H]

Identifier: 238026009

GI number: 238026009

Start: 354389

End: 355030

Strand: Direct

Name: hisH [H]

Synonym: bglu_1g03350

Alternate gene names: 238026009

Gene position: 354389-355030 (Clockwise)

Preceding gene: 238026008

Following gene: 238026010

Centisome position: 9.07

GC content: 69.31

Gene sequence:

>642_bases
ATGAAAACTTCGATTGCGATTGTGGATTATGGGATGGGCAACCTGCGCTCGGTGGCCCAGGCGCTGATGAAGGCCGAGCC
GGAGGCGCAGGTGGCGATCGTCGATCGTCCCGAGGCGATCCGCGCGGCCGACCGCGTGGTGCTGCCGGGGCAGGGCGCGA
TGCCCGACTGCATGCGCTGCCTGGGCGAATCGGGGCTGCAGGAGGCGCTGCTCGAGGCCTCGCGCAGCAAGCCGCTGCTG
GGCGTGTGCGTCGGCGAGCAGATGCTGTTCGACTGGAGCGAGGAGGGCGCCACGCCGGGCCTCGGGCTGCTGCCGGGCAA
GGCGCTGCGCTTCGATCTGGCCGGCCGCGTGCAGGATGACGGCTCGCGCTTCAAGGTGCCCCAGATGGGCTGGAACCGGG
TGCGCCAGACGCTCGCGCACCCGCTCTGGGCGGGCGTGCCGGACCAGGCGTTCTTCTACTTCGTCCACAGCTACTACGTG
GTGCCGGACGACGCGGCGCACGCCGCCGGCGAGACCTCGTATGGCGAGCCGTTCGTCTCGGCGGTCGCGCGCGACAATAT
CTTCGCGACCCAATTTCACCCGGAAAAGAGCGCCGAGGTCGGCCTGCGGCTCTACCGCAATTTCGTGCACTGGCAGCCCT
GA

Upstream 100 bases:

>100_bases
CCGGCATTAATATTGCGACGCGACTGATGGGATTGATGCTCTCGGCGCTGGCGGTGGAATTCATCGTCGACGGACTGAAG
GCTTTGCTGCCTGCACTGAA

Downstream 100 bases:

>100_bases
GCGTCGCGCGGCCGCCACGCCGAACCGGCCGGACCTGCCCGTGGCGGCGCGCGCGCGCGTCAAAACCCGGAAAGACTTGT
ACTAAACTAGCAGACAGCGC

Product: imidazole glycerol phosphate synthase subunit HisH

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKTSIAIVDYGMGNLRSVAQALMKAEPEAQVAIVDRPEAIRAADRVVLPGQGAMPDCMRCLGESGLQEALLEASRSKPLL
GVCVGEQMLFDWSEEGATPGLGLLPGKALRFDLAGRVQDDGSRFKVPQMGWNRVRQTLAHPLWAGVPDQAFFYFVHSYYV
VPDDAAHAAGETSYGEPFVSAVARDNIFATQFHPEKSAEVGLRLYRNFVHWQP

Sequences:

>Translated_213_residues
MKTSIAIVDYGMGNLRSVAQALMKAEPEAQVAIVDRPEAIRAADRVVLPGQGAMPDCMRCLGESGLQEALLEASRSKPLL
GVCVGEQMLFDWSEEGATPGLGLLPGKALRFDLAGRVQDDGSRFKVPQMGWNRVRQTLAHPLWAGVPDQAFFYFVHSYYV
VPDDAAHAAGETSYGEPFVSAVARDNIFATQFHPEKSAEVGLRLYRNFVHWQP
>Mature_213_residues
MKTSIAIVDYGMGNLRSVAQALMKAEPEAQVAIVDRPEAIRAADRVVLPGQGAMPDCMRCLGESGLQEALLEASRSKPLL
GVCVGEQMLFDWSEEGATPGLGLLPGKALRFDLAGRVQDDGSRFKVPQMGWNRVRQTLAHPLWAGVPDQAFFYFVHSYYV
VPDDAAHAAGETSYGEPFVSAVARDNIFATQFHPEKSAEVGLRLYRNFVHWQP

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR [H]

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788334, Length=211, Percent_Identity=35.5450236966825, Blast_Score=118, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319725, Length=219, Percent_Identity=32.8767123287671, Blast_Score=106, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226 [H]

Pfam domain/function: PF00117 GATase [H]

EC number: 2.4.2.- [C]

Molecular weight: Translated: 23363; Mature: 23363

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSIAIVDYGMGNLRSVAQALMKAEPEAQVAIVDRPEAIRAADRVVLPGQGAMPDCMRC
CCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHCEEEECCCCCCHHHHHH
LGESGLQEALLEASRSKPLLGVCVGEQMLFDWSEEGATPGLGLLPGKALRFDLAGRVQDD
HCCCCHHHHHHHHHCCCCEEEEEECHHHHEECHHCCCCCCCCCCCCCEEEEEECCCCCCC
GSRFKVPQMGWNRVRQTLAHPLWAGVPDQAFFYFVHSYYVVPDDAAHAAGETSYGEPFVS
CCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHEEECCCHHHHHCCCCCCCCHHHH
AVARDNIFATQFHPEKSAEVGLRLYRNFVHWQP
HHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKTSIAIVDYGMGNLRSVAQALMKAEPEAQVAIVDRPEAIRAADRVVLPGQGAMPDCMRC
CCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHHCEEEECCCCCCHHHHHH
LGESGLQEALLEASRSKPLLGVCVGEQMLFDWSEEGATPGLGLLPGKALRFDLAGRVQDD
HCCCCHHHHHHHHHCCCCEEEEEECHHHHEECHHCCCCCCCCCCCCCEEEEEECCCCCCC
GSRFKVPQMGWNRVRQTLAHPLWAGVPDQAFFYFVHSYYVVPDDAAHAAGETSYGEPFVS
CCEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHEEECCCHHHHHCCCCCCCCHHHH
AVARDNIFATQFHPEKSAEVGLRLYRNFVHWQP
HHHHCCEEEEEECCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12754231 [H]