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The map label for this gene is clcD [H]

Identifier: 238025877

GI number: 238025877

Start: 213697

End: 214392

Strand: Reverse

Name: clcD [H]

Synonym: bglu_1g02030

Alternate gene names: 238025877

Gene position: 214392-213697 (Counterclockwise)

Preceding gene: 238025879

Following gene: 238025876

Centisome position: 5.49

GC content: 69.4

Gene sequence:

>696_bases
ATGACTTCGCAATGGATCGACATCCCGGTCGGCAATGAAACGTTCGGTGGCTACCTTGCCCTGCCCAAGGGCGGCAAGGG
ACCGGCCGTGGTGATCATTCAGGAAATCTTCGGCGTGAACGGCCACATCCGTTCGGTCGCCGAGCAATATGCGGCCGACG
GCTACGTCGCGCTCGCCCCGGACATCTTCTGGCGCACCCAGCCGCGCGTGGAGCTCGGTTACGAAGGCGCCGACTTCAAC
AAGGGCCTGGAGCTGCTGCAGAAGACCGATCCGCACCAGGCCGCGGCCGACATCGGCCACGCGGTCGCCGCGCTGCGCGC
GCGCCCCGAGGTGGAAGGCAAGCGTGTGGCCGGGATCGGCTACTGCTTCGGCGGCCGGCTCGCCTACCTGGCCGCGGCGG
CCGGCGCGCTCGACGTGGCGGTCGCCTACTATGGCGGCGGCATCCAGAACCATCTCGACGTCGCGAACCACGTCACGCAG
CCGATCCAGTTCCACTACGCCGGCCTCGACGACCACATCCCGGCCGAGGCGGTCGAGCAGGTCAGGGCCGCGTTCGGCGG
CCGCGCGCACGCGCAGGTGCACTGGTATCCGGATGCCGGCCACGGCTTCAACTGCAACGTGCGCGCCTCCTACAACCAGC
GCGCGGCGGCGCTCGCGCACGGCCGCACGCTGACCTTCCTCGCCGAGCAGCTGTAA

Upstream 100 bases:

>100_bases
GGCGCGCTGCAGCGCGGCACGAACGATGGCACACTGACGCCGCCCTGTTCCCCTCGCGCGTGCCCTTCCCTGCGGCGCGC
GCTTCGTCTGGAGACCCGCT

Downstream 100 bases:

>100_bases
GCTCGTCGCACCGGCCCGCGCGGGCCGGCGTCCGCACGCGCTGGCCGGCCGCCGGCGCGACGCCGGCGCCGCGCGTGGCC
CGATTCCGACTCGAACAACG

Product: putative dienelactone hydrolase

Products: NA

Alternate protein names: Dienelactone hydrolase; DLH [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MTSQWIDIPVGNETFGGYLALPKGGKGPAVVIIQEIFGVNGHIRSVAEQYAADGYVALAPDIFWRTQPRVELGYEGADFN
KGLELLQKTDPHQAAADIGHAVAALRARPEVEGKRVAGIGYCFGGRLAYLAAAAGALDVAVAYYGGGIQNHLDVANHVTQ
PIQFHYAGLDDHIPAEAVEQVRAAFGGRAHAQVHWYPDAGHGFNCNVRASYNQRAAALAHGRTLTFLAEQL

Sequences:

>Translated_231_residues
MTSQWIDIPVGNETFGGYLALPKGGKGPAVVIIQEIFGVNGHIRSVAEQYAADGYVALAPDIFWRTQPRVELGYEGADFN
KGLELLQKTDPHQAAADIGHAVAALRARPEVEGKRVAGIGYCFGGRLAYLAAAAGALDVAVAYYGGGIQNHLDVANHVTQ
PIQFHYAGLDDHIPAEAVEQVRAAFGGRAHAQVHWYPDAGHGFNCNVRASYNQRAAALAHGRTLTFLAEQL
>Mature_230_residues
TSQWIDIPVGNETFGGYLALPKGGKGPAVVIIQEIFGVNGHIRSVAEQYAADGYVALAPDIFWRTQPRVELGYEGADFNK
GLELLQKTDPHQAAADIGHAVAALRARPEVEGKRVAGIGYCFGGRLAYLAAAAGALDVAVAYYGGGIQNHLDVANHVTQP
IQFHYAGLDDHIPAEAVEQVRAAFGGRAHAQVHWYPDAGHGFNCNVRASYNQRAAALAHGRTLTFLAEQL

Specific function: Ring cleavage of cyclic ester dienelactone to produce maleylacetate [H]

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=235, Percent_Identity=34.8936170212766, Blast_Score=120, Evalue=1e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 24698; Mature: 24567

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSQWIDIPVGNETFGGYLALPKGGKGPAVVIIQEIFGVNGHIRSVAEQYAADGYVALAP
CCCCEEEECCCCCCCCCEEECCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHCCCEEEECC
DIFWRTQPRVELGYEGADFNKGLELLQKTDPHQAAADIGHAVAALRARPEVEGKRVAGIG
CHHCCCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
YCFGGRLAYLAAAAGALDVAVAYYGGGIQNHLDVANHVTQPIQFHYAGLDDHIPAEAVEQ
EEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHH
VRAAFGGRAHAQVHWYPDAGHGFNCNVRASYNQRAAALAHGRTLTFLAEQL
HHHHHCCCCEEEEEEECCCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHCC
>Mature Secondary Structure 
TSQWIDIPVGNETFGGYLALPKGGKGPAVVIIQEIFGVNGHIRSVAEQYAADGYVALAP
CCCEEEECCCCCCCCCEEECCCCCCCCEEEEEEHHHCCCCHHHHHHHHHHCCCEEEECC
DIFWRTQPRVELGYEGADFNKGLELLQKTDPHQAAADIGHAVAALRARPEVEGKRVAGIG
CHHCCCCCCEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEE
YCFGGRLAYLAAAAGALDVAVAYYGGGIQNHLDVANHVTQPIQFHYAGLDDHIPAEAVEQ
EEHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHH
VRAAFGGRAHAQVHWYPDAGHGFNCNVRASYNQRAAALAHGRTLTFLAEQL
HHHHHCCCCEEEEEEECCCCCCCCEEEEECCCCHHHHHHCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3299368 [H]