Click here to switch to the map view.

The map label for this gene is gcvH [H]

Identifier: 238025778

GI number: 238025778

Start: 115151

End: 115531

Strand: Reverse

Name: gcvH [H]

Synonym: bglu_1g01020

Alternate gene names: 238025778

Gene position: 115531-115151 (Counterclockwise)

Preceding gene: 238025779

Following gene: 238025777

Centisome position: 2.96

GC content: 65.35

Gene sequence:

>381_bases
ATGAGCAACGTCCCGGCCGATCTGAAGTACACCGACGAACACGAGTGGATCCGCACCGAGGCGGACGGCACGCTGACGAT
CGGCATCACCGATCACGCGCAAACCACGCTCGGCGACATCGTCTTCCTCGAACTGCCGCAAGTCGGCAAGCACGCCGACG
CGGGCGACCCGGTGGGCGTCGTCGAATCGGTGAAAGCCGCCTCGGACATCTACGCACCGGTGTCGGGCGAGATCATCGCC
ATCAACGAGGATGCCGTCGACACGCCGGAATCGGTCAACCAGGACGCCTACGACGCCTGGCTCTTCAAGGTCAGGCTCGC
GCCGGGCGCCAGCACCGGCGCGCTGCTCGACGCCCAGGCCTACAAGAAGCTGATCGACTAA

Upstream 100 bases:

>100_bases
AAACTCCCGTTCGTGCGCAACGGCAAGGTGCTGGCCGCCTGACGGCCGCCCGCCCTGCGCACGGCATTCAACGCACCCAT
CACCATCTCAGGAGCATCCG

Downstream 100 bases:

>100_bases
CCCTCTGTCCACCCAGGCGGCGCGCCCAGCCACGGCGCGCGCCGCAACCCGCCGGAACGCCATGAAGCTCGAACACCCGG
ATCACCTGATGAACCGCACG

Product: glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 126; Mature: 125

Protein sequence:

>126_residues
MSNVPADLKYTDEHEWIRTEADGTLTIGITDHAQTTLGDIVFLELPQVGKHADAGDPVGVVESVKAASDIYAPVSGEIIA
INEDAVDTPESVNQDAYDAWLFKVRLAPGASTGALLDAQAYKKLID

Sequences:

>Translated_126_residues
MSNVPADLKYTDEHEWIRTEADGTLTIGITDHAQTTLGDIVFLELPQVGKHADAGDPVGVVESVKAASDIYAPVSGEIIA
INEDAVDTPESVNQDAYDAWLFKVRLAPGASTGALLDAQAYKKLID
>Mature_125_residues
SNVPADLKYTDEHEWIRTEADGTLTIGITDHAQTTLGDIVFLELPQVGKHADAGDPVGVVESVKAASDIYAPVSGEIIAI
NEDAVDTPESVNQDAYDAWLFKVRLAPGASTGALLDAQAYKKLID

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=119, Percent_Identity=43.6974789915966, Blast_Score=108, Evalue=1e-24,
Organism=Homo sapiens, GI89057342, Length=119, Percent_Identity=42.8571428571429, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1789271, Length=127, Percent_Identity=58.2677165354331, Blast_Score=155, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17551294, Length=116, Percent_Identity=43.9655172413793, Blast_Score=99, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI17507493, Length=116, Percent_Identity=42.2413793103448, Blast_Score=96, Evalue=5e-21,
Organism=Saccharomyces cerevisiae, GI6319272, Length=128, Percent_Identity=38.28125, Blast_Score=97, Evalue=9e-22,
Organism=Drosophila melanogaster, GI17865652, Length=121, Percent_Identity=40.495867768595, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 13438; Mature: 13307

Theoretical pI: Translated: 3.99; Mature: 3.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNVPADLKYTDEHEWIRTEADGTLTIGITDHAQTTLGDIVFLELPQVGKHADAGDPVGV
CCCCCCCCEECCCCCEEEECCCCEEEEEECCCCCHHHCCEEEEECCCCCCCCCCCCCCHH
VESVKAASDIYAPVSGEIIAINEDAVDTPESVNQDAYDAWLFKVRLAPGASTGALLDAQA
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEECHHH
YKKLID
HHHHCC
>Mature Secondary Structure 
SNVPADLKYTDEHEWIRTEADGTLTIGITDHAQTTLGDIVFLELPQVGKHADAGDPVGV
CCCCCCCEECCCCCEEEECCCCEEEEEECCCCCHHHCCEEEEECCCCCCCCCCCCCCHH
VESVKAASDIYAPVSGEIIAINEDAVDTPESVNQDAYDAWLFKVRLAPGASTGALLDAQA
HHHHHHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCEECHHH
YKKLID
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA