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The map label for this gene is fliM [H]

Identifier: 238025700

GI number: 238025700

Start: 21767

End: 22765

Strand: Reverse

Name: fliM [H]

Synonym: bglu_1g00220

Alternate gene names: 238025700

Gene position: 22765-21767 (Counterclockwise)

Preceding gene: 238025701

Following gene: 238025699

Centisome position: 0.58

GC content: 63.26

Gene sequence:

>999_bases
ATGGGCCACGAAGAATTCATGTCGCAGGAGGAGGTCGATGCCCTCCTCAAAGGTGTCACGGGAGAGGTTGACGCCAGCGA
CGAACCGGTCGAGCAGAAAGGCGTCCGCCCGTACAACCTCGCGACGCAGGAGCGGATCGTCCGCGGCCGGATGCCCGGCC
TCGAGATCATCAACGACCGTTTCGCGCGGCTGCTGCGCGTCGGCATCTTCAACTTCATGCGCCGCACGGCCGAAATCTCG
GTCAGCCAGGTGAAGGTGCAGAAGTACAGCGAGTTCACGCGCAATCTGCCGATCCCGACCAACCTGAACCTGGTCCACAT
CAAGCCGCTGCGCGGCACCTCGCTGTTCGTGTTCGATCCGAACCTGGTGTTCTTCGTGGTGGACAACCTGTTCGGCGGTG
ACGGGCGCTTCCACACGCGCGTGGAAGGCCGCGACTTCACGCAGACGGAGATGCGCATCATCAGCCGGCTGCTGCACCTG
GTGTTCGAGCACTACTCGGTGGCCTGGAAGAGCGTGCGGCCGCTGCAGTTCGAGCACGTGCGCTCGGAGATGCATACGCA
GTTCGCCAACGTCGCCACGCCCAACGAGATCGTGATCGTCACGCAGTTCTCGATCGAGTTCGGGCCGACCGGCGGCACGC
TGCACATCTGCATGCCGTACTCGATGATCGAGCCGATCCGCGACGTGCTGAGCTCGCCGATCCAGGGCGAGGCGCTCGAG
GTGGACCGCCGCTGGGTGCGGGTGCTCTCGCAGCAGGTGCAGTCCGCCGAGGTCGAACTCACGGCCAACCTCACCGAGAT
GCCGAGCACCTTCGAGAAGATCCTCAACCTGCGGGTCGGCGACGTGCTGCCGATCGACGTCGAGGAATCGATCATCGCGA
AGGTCGACGGCGTGCCGGTGATGGAATGCGGCTACGGGATTTTCAATGGGCAATATGCGCTGCGGGTGAACCGGATGATC
AGCGCGAGCGATACGATGAAGGAAGGTGGATATGACTGA

Upstream 100 bases:

>100_bases
GCTGATCGAGAAACCGACGCAGCCTGGCAATCGCAGCGCACGCGTCGACGCGGTGCTGTTTACCGATTTCGTCGTCCAGT
AACGCCGCCAAGGGAGCGCC

Downstream 100 bases:

>100_bases
GCTGAACCCGACGCCCGAAGGCAAGCAAGCCGGCCCCGGTGAGGAGTCGTCGCTGGACGATTGGGCCGCCGCGCTCGCCG
AGCAGAACGACCAGCCGATC

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MGHEEFMSQEEVDALLKGVTGEVDASDEPVEQKGVRPYNLATQERIVRGRMPGLEIINDRFARLLRVGIFNFMRRTAEIS
VSQVKVQKYSEFTRNLPIPTNLNLVHIKPLRGTSLFVFDPNLVFFVVDNLFGGDGRFHTRVEGRDFTQTEMRIISRLLHL
VFEHYSVAWKSVRPLQFEHVRSEMHTQFANVATPNEIVIVTQFSIEFGPTGGTLHICMPYSMIEPIRDVLSSPIQGEALE
VDRRWVRVLSQQVQSAEVELTANLTEMPSTFEKILNLRVGDVLPIDVEESIIAKVDGVPVMECGYGIFNGQYALRVNRMI
SASDTMKEGGYD

Sequences:

>Translated_332_residues
MGHEEFMSQEEVDALLKGVTGEVDASDEPVEQKGVRPYNLATQERIVRGRMPGLEIINDRFARLLRVGIFNFMRRTAEIS
VSQVKVQKYSEFTRNLPIPTNLNLVHIKPLRGTSLFVFDPNLVFFVVDNLFGGDGRFHTRVEGRDFTQTEMRIISRLLHL
VFEHYSVAWKSVRPLQFEHVRSEMHTQFANVATPNEIVIVTQFSIEFGPTGGTLHICMPYSMIEPIRDVLSSPIQGEALE
VDRRWVRVLSQQVQSAEVELTANLTEMPSTFEKILNLRVGDVLPIDVEESIIAKVDGVPVMECGYGIFNGQYALRVNRMI
SASDTMKEGGYD
>Mature_331_residues
GHEEFMSQEEVDALLKGVTGEVDASDEPVEQKGVRPYNLATQERIVRGRMPGLEIINDRFARLLRVGIFNFMRRTAEISV
SQVKVQKYSEFTRNLPIPTNLNLVHIKPLRGTSLFVFDPNLVFFVVDNLFGGDGRFHTRVEGRDFTQTEMRIISRLLHLV
FEHYSVAWKSVRPLQFEHVRSEMHTQFANVATPNEIVIVTQFSIEFGPTGGTLHICMPYSMIEPIRDVLSSPIQGEALEV
DRRWVRVLSQQVQSAEVELTANLTEMPSTFEKILNLRVGDVLPIDVEESIIAKVDGVPVMECGYGIFNGQYALRVNRMIS
ASDTMKEGGYD

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=326, Percent_Identity=51.2269938650307, Blast_Score=343, Evalue=8e-96,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 37664; Mature: 37533

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHEEFMSQEEVDALLKGVTGEVDASDEPVEQKGVRPYNLATQERIVRGRMPGLEIINDR
CCHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHH
FARLLRVGIFNFMRRTAEISVSQVKVQKYSEFTRNLPIPTNLNLVHIKPLRGTSLFVFDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECC
NLVFFVVDNLFGGDGRFHTRVEGRDFTQTEMRIISRLLHLVFEHYSVAWKSVRPLQFEHV
CEEEEEEEHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RSEMHTQFANVATPNEIVIVTQFSIEFGPTGGTLHICMPYSMIEPIRDVLSSPIQGEALE
HHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCEEEEEECHHHHHHHHHHHCCCCCCCHHH
VDRRWVRVLSQQVQSAEVELTANLTEMPSTFEKILNLRVGDVLPIDVEESIIAKVDGVPV
HHHHHHHHHHHHHHHCEEEEEECHHHCHHHHHHHHCCCCCCEEECCCCHHHHHHCCCCEE
MECGYGIFNGQYALRVNRMISASDTMKEGGYD
HHCCCCEECCEEEEEEEEHHCCCHHHHCCCCC
>Mature Secondary Structure 
GHEEFMSQEEVDALLKGVTGEVDASDEPVEQKGVRPYNLATQERIVRGRMPGLEIINDR
CHHHHCCHHHHHHHHHCCCCCCCCCCCCHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHH
FARLLRVGIFNFMRRTAEISVSQVKVQKYSEFTRNLPIPTNLNLVHIKPLRGTSLFVFDP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEECC
NLVFFVVDNLFGGDGRFHTRVEGRDFTQTEMRIISRLLHLVFEHYSVAWKSVRPLQFEHV
CEEEEEEEHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RSEMHTQFANVATPNEIVIVTQFSIEFGPTGGTLHICMPYSMIEPIRDVLSSPIQGEALE
HHHHHHHHHCCCCCCEEEEEEEEEEEECCCCCEEEEEECHHHHHHHHHHHCCCCCCCHHH
VDRRWVRVLSQQVQSAEVELTANLTEMPSTFEKILNLRVGDVLPIDVEESIIAKVDGVPV
HHHHHHHHHHHHHHHCEEEEEECHHHCHHHHHHHHCCCCCCEEECCCCHHHHHHCCCCEE
MECGYGIFNGQYALRVNRMISASDTMKEGGYD
HHCCCCEECCEEEEEEEEHHCCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 3519573; 9097040; 9278503; 2651416 [H]