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The map label for this gene is 238025371

Identifier: 238025371

GI number: 238025371

Start: 2533536

End: 2534810

Strand: Direct

Name: 238025371

Synonym: bglu_2g20470

Alternate gene names: NA

Gene position: 2533536-2534810 (Clockwise)

Preceding gene: 238025366

Following gene: 238025379

Centisome position: 89.61

GC content: 71.61

Gene sequence:

>1275_bases
ATGTGGTACGACCTGATCGAACAGCAAGCCCGCTGGTGGCGCGCATGGCGCGCGGCGACGGCGTGGACCGGCGCCCTCGG
CGACGCGACGGCGGCGGCCTGCTGCGCCGACCTGCTCGAACCGATGCTGGGCTGGCAGGACGGCATGCCGCCGTTCGAGA
TCGATACGATCGTGCTGAAGGGACGCGCCGTCGGCATCGACAGCACGGTCGCGGCATGCATGCCGTTTTGCGCGCTGCGC
CGCTTCGCCCGGCGCAACGCGCGGCGCGCGATCCTGCTGTGCGTGCCGCTGGCCGGCCATGCCGCGGTGATGATGCGGGA
CACCGTCGAGGCGCTCCTGGAGGATGGCGACGTGTTCGTCACCGAATGGGCCAACGCGCGCGACGTGCCGCTCGATGCGG
GCCGCTTCGGTCTCGACGAGTACGTCGCGATGCTGGATGGCTTCGTGGGCTGGCTGCTGACCGGCACCCGGCCGGTGCAC
GTGGTCGCCGTCTGCCAGGCGGCGGTCCCCGGCCTGGGCGCGATCGCGCTGCGGGCCCAGCGCGGCGCCCCGATACCGGC
CAGCCTCACCCTGATCGGCGGCCCGCTCGACGCACGCATCCATCCAAGCGCGCTCGGCATCGCGGCCTGCTCGCATTCGC
TGGCGTGGTGCGACAGCAACCTGATCGACGTGGTGCCGCAAGGGTTTGCCGGCCGGGGCAGAAAGGTGTTTCCGGCATAT
CTCCAGCGCGCGGAGATCGCCTTCGTCTACCCGCATCGCTACCTCGAGATGGCGCAGCGCTGCCGGTCGGCCGCCTCGAG
CGGCGACGCCCGCGCCTACGAGGGCGCACGGCGGGCACTGAACGAATATGCGGCCTTGCTGGACATGCCAGCCGAGTATT
TCCTCGATACCGTCGATTCGGTATTCCAGCGCGCCAGCCTCGCGCAGGGCGACTGGCGGGTAGGCGGCGTGCGCGTCGAT
CCCACGGCCCTGTGCGATCTCGACCTGCTGACCGTGGAGGGCGGCCGCGACAGCGTCACCGGCGCCGGCCAGACCCATGC
CGCGCTGGCGATGTGCGGCGGTCGGCCCGGCCGGCAACGCATGCGCCTCGACGTCGAGGACTGCGATCATTACGGCTTGT
TCACCGGCCCGCGCTGGCGCGGTGAGGTCCATCACGCGCTGCGGGCGAGGTTCGCGCACGCCGAACTCGCGCGGGCCGCT
CGAGCGGTGCCGGATCCGCTCGACCGCCCCATTGACGCCGATTCATCGGGAATCGGCCGCTCGTGCGCGCGGTGA

Upstream 100 bases:

>100_bases
CCGCGATACCGCGATCTCGCTGACTCAGGTCAAGGCCGGCCCGGAGCGGGAGACGACAGTGTGCCGGTGAGCGCTCCGGC
TCGTGTCGAGGATCTTTGCG

Downstream 100 bases:

>100_bases
CTCAGGCGGAAAAGTCGATCACCATGCGGCCCTGGATATCGCCCTGATGCATCTTCGAGAAGATGTCGTTGATATCCTCG
AGCTTCGCGGTGCTGACCGT

Product: polyhydroxyalkanoate depolymerase, intracellular

Products: NA

Alternate protein names: Intracellular PHB Depolymerase; Polyhydroxyalkanoate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase; PHB Depolymerase; Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Intracellular Poly(3-Hydroxybutyrate) Depolymerase; Intracellular Polyhydroxyalkanoate Depolymerase; Polyhydroxyalkanoate Depolymerase Intracellular Subfamily; Poly(3-Hydroxyalkanoate) Depolymerase; PHB De-Polymerase Domain-Containing Protein; Poly(3-Hydroxybutyrate) Depolymerase; Intracellular PHB Depolymerase Protein; Poly-Beta-Hydroxyalkanoate Depolymerase-Like Protein; Intracellular Poly[D(-)-3-Hydroxybutyrate] Depolymerase; Poly[D(-)-3-Hydroxybutyrate](Phb) Depolymerase; Poly-Beta-Hydroxyalkanoate/Butyrate Depolymerase; Poly-Beta-Hydroxyalkanoate Depolymerase Protein; Poly[D(-)-3-Hydroxyalkanoate] Depolymerase; Polyhydroxybutanoate Depolymerase; Poly-Beta-Hydroxybutyrate Depolymerase

Number of amino acids: Translated: 424; Mature: 424

Protein sequence:

>424_residues
MWYDLIEQQARWWRAWRAATAWTGALGDATAAACCADLLEPMLGWQDGMPPFEIDTIVLKGRAVGIDSTVAACMPFCALR
RFARRNARRAILLCVPLAGHAAVMMRDTVEALLEDGDVFVTEWANARDVPLDAGRFGLDEYVAMLDGFVGWLLTGTRPVH
VVAVCQAAVPGLGAIALRAQRGAPIPASLTLIGGPLDARIHPSALGIAACSHSLAWCDSNLIDVVPQGFAGRGRKVFPAY
LQRAEIAFVYPHRYLEMAQRCRSAASSGDARAYEGARRALNEYAALLDMPAEYFLDTVDSVFQRASLAQGDWRVGGVRVD
PTALCDLDLLTVEGGRDSVTGAGQTHAALAMCGGRPGRQRMRLDVEDCDHYGLFTGPRWRGEVHHALRARFAHAELARAA
RAVPDPLDRPIDADSSGIGRSCAR

Sequences:

>Translated_424_residues
MWYDLIEQQARWWRAWRAATAWTGALGDATAAACCADLLEPMLGWQDGMPPFEIDTIVLKGRAVGIDSTVAACMPFCALR
RFARRNARRAILLCVPLAGHAAVMMRDTVEALLEDGDVFVTEWANARDVPLDAGRFGLDEYVAMLDGFVGWLLTGTRPVH
VVAVCQAAVPGLGAIALRAQRGAPIPASLTLIGGPLDARIHPSALGIAACSHSLAWCDSNLIDVVPQGFAGRGRKVFPAY
LQRAEIAFVYPHRYLEMAQRCRSAASSGDARAYEGARRALNEYAALLDMPAEYFLDTVDSVFQRASLAQGDWRVGGVRVD
PTALCDLDLLTVEGGRDSVTGAGQTHAALAMCGGRPGRQRMRLDVEDCDHYGLFTGPRWRGEVHHALRARFAHAELARAA
RAVPDPLDRPIDADSSGIGRSCAR
>Mature_424_residues
MWYDLIEQQARWWRAWRAATAWTGALGDATAAACCADLLEPMLGWQDGMPPFEIDTIVLKGRAVGIDSTVAACMPFCALR
RFARRNARRAILLCVPLAGHAAVMMRDTVEALLEDGDVFVTEWANARDVPLDAGRFGLDEYVAMLDGFVGWLLTGTRPVH
VVAVCQAAVPGLGAIALRAQRGAPIPASLTLIGGPLDARIHPSALGIAACSHSLAWCDSNLIDVVPQGFAGRGRKVFPAY
LQRAEIAFVYPHRYLEMAQRCRSAASSGDARAYEGARRALNEYAALLDMPAEYFLDTVDSVFQRASLAQGDWRVGGVRVD
PTALCDLDLLTVEGGRDSVTGAGQTHAALAMCGGRPGRQRMRLDVEDCDHYGLFTGPRWRGEVHHALRARFAHAELARAA
RAVPDPLDRPIDADSSGIGRSCAR

Specific function: Unknown

COG id: COG4553

COG function: function code I; Poly-beta-hydroxyalkanoate depolymerase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.1.1.-

Molecular weight: Translated: 45891; Mature: 45891

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWYDLIEQQARWWRAWRAATAWTGALGDATAAACCADLLEPMLGWQDGMPPFEIDTIVLK
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
GRAVGIDSTVAACMPFCALRRFARRNARRAILLCVPLAGHAAVMMRDTVEALLEDGDVFV
CEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEE
TEWANARDVPLDAGRFGLDEYVAMLDGFVGWLLTGTRPVHVVAVCQAAVPGLGAIALRAQ
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCEEEECC
RGAPIPASLTLIGGPLDARIHPSALGIAACSHSLAWCDSNLIDVVPQGFAGRGRKVFPAY
CCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCHHHHCCCCHHHHCCCCCCCCCCEECHHH
LQRAEIAFVYPHRYLEMAQRCRSAASSGDARAYEGARRALNEYAALLDMPAEYFLDTVDS
HCCCCEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VFQRASLAQGDWRVGGVRVDPTALCDLDLLTVEGGRDSVTGAGQTHAALAMCGGRPGRQR
HHHHHHCCCCCEEECCEEECCHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCHH
MRLDVEDCDHYGLFTGPRWRGEVHHALRARFAHAELARAARAVPDPLDRPIDADSSGIGR
EEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
SCAR
HCCC
>Mature Secondary Structure
MWYDLIEQQARWWRAWRAATAWTGALGDATAAACCADLLEPMLGWQDGMPPFEIDTIVLK
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEE
GRAVGIDSTVAACMPFCALRRFARRNARRAILLCVPLAGHAAVMMRDTVEALLEDGDVFV
CEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHCCCCEEE
TEWANARDVPLDAGRFGLDEYVAMLDGFVGWLLTGTRPVHVVAVCQAAVPGLGAIALRAQ
EECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHCCCCCCEEEECC
RGAPIPASLTLIGGPLDARIHPSALGIAACSHSLAWCDSNLIDVVPQGFAGRGRKVFPAY
CCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCHHHHCCCCHHHHCCCCCCCCCCEECHHH
LQRAEIAFVYPHRYLEMAQRCRSAASSGDARAYEGARRALNEYAALLDMPAEYFLDTVDS
HCCCCEEEECCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHH
VFQRASLAQGDWRVGGVRVDPTALCDLDLLTVEGGRDSVTGAGQTHAALAMCGGRPGRQR
HHHHHHCCCCCEEECCEEECCHHHCCEEEEEECCCCCCCCCCCCHHHHHHHHCCCCCCHH
MRLDVEDCDHYGLFTGPRWRGEVHHALRARFAHAELARAARAVPDPLDRPIDADSSGIGR
EEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCH
SCAR
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA