Click here to switch to the map view.

The map label for this gene is plcN [H]

Identifier: 238025323

GI number: 238025323

Start: 2483034

End: 2485223

Strand: Direct

Name: plcN [H]

Synonym: bglu_2g19970

Alternate gene names: 238025323

Gene position: 2483034-2485223 (Clockwise)

Preceding gene: 238025322

Following gene: 238025324

Centisome position: 87.82

GC content: 66.85

Gene sequence:

>2190_bases
ATGTCTACCTACAATCGACGACGCTTCCTGCAGACCGCCGCCTCGACGGCCGGCGCGGCAGCAGCCCTGACGGTGTTCCC
CGATGCCATCCGCAAGGCGCTCGCCATTCCGGCGGCGTCGGGCACGGGCTCGATCCGGGACGTGCAGCACATCGTGGTGC
TGATGCAGGAAAACCGCTCGTTCGACCATTACTTCGGCCACCTGCGCGGCGTGCGCGGCTACAACGACCGCTTCCCGATC
CCGCTGGCCAGCGGCCGGCCGGTCTGGTTCCAGCCATCCAAGGCCGATCCGGCCACGCCGGTGCTGCCGTTCCGCTACGA
CGTCAACAAGGTCAGCGAATGCCTGGGCGACCTGGACCACAGCTGGTACCCGACCCACCACGCGATCAACAACGGCCGCA
TGGACGCCTGGCCGGCCAACAAGTCCGACCTGACGATGGGCTACCACGTGCGCGAGGACATCCCGTTCCATTACGCGCTG
GCCGATGCGTTCACGATCTGCGATCACTACTTCTGCTCGATGCCGGGGCCGACCCACCCGAACCGCACCTATCTGATGAC
CGGCATGGTGGACCCGACCGGCAAGCAGGGCGGCCCGCTGCTGGACAACAACGACGCCATCGACGACGACCTGCCGCCGA
AGTGGGATCTGCTGACCTGGACCACCTACCCGGAGCGGCTGCAGGCGGCCGGCATCTCGTGGCAGCTCTACCAGCAGGGC
GTAAACGGCAACGACCCGGTGAACGGCAACTACGGCACCAACATGCTGCCGAACTTCGCCAACTTCATCAACGCGCCGGC
CGGCTCGGCGCTGCAAACGCGCGGCAACGCCGTGCGCACCCTGGCCGACCTCAAGAACGACGTGCTGGCCAACCAGCTGC
CGCAGGTCTGCTGGCTGTGCCCGCCGGCGATCTACTCCGAGCACCCGCGCTATACGCCCGCCTATGGCGCGACCTATATT
GCCCAGATCCTCGACGCGCTGACCTCGAACCCGGCGGTGTGGAGCAAAACCGTCTTCTTTCTGATGTACGACGAGAACGA
CGGCTTCTTCGACCACCTCGCCCCGCCGCAACCGCCCACCCATCGCGGCCAGGGCCTGTCGACCGTGGACGTCAGCGCCG
AGATCCACAACGTGGTGAACCCGCGGCGCGGCGGCAGCTACACGGCCGACAACCTGCCCTACGGCATGGGTCCGCGCGTG
CCGATGATCGTGATCTCGCCATGGACCAAGGGCGGCTACGTGAACTCGCAGGTCTTCGACCACACCTCGGTGATCCGCTT
CATGGAGCAGCGCTTCGGCGTGATGGAGCCCAACATCAGCCCGTGGCGCCGCGCCGTCTCGGGCGACCTGCTGAGCTGCT
TCGACTTCGGCACGCCGGACGCGGCCTTCCCGGTACTGCCCGATACCAGCAACTACCAGGCGAAATCCGACGCGTCCTGC
CAGAACCCGAAGGCGGCGGTGCCCGCGGTGCCGAGCCCCGCCTCGATCAAGGAGCAGGAAGCCGGCGTGCGTGCCGCGCG
CGCCCTGCCCTACGAACTGCACGCCAACGGCCGCGAAGCGATCAAGGAAAACGAATTCCAGATCCACTTCTCGAACACGG
GCAAGGTGGGCGCGTGCTTCTACGTGTACTCGACCAACCGCAGCGACGGCCCATGGCGCTACACGGTGGAAGCCGGCAAG
TCGATCGCCGACACCTTCAATCTGGCCGGCACGGGTGGCGTCTACAACTTCCTGGTCTATGGCCCGAACGGTTTCGTGCG
CCATTTCGTGGGTGCCATCCCGCAGGACCAGAAGACCCGGCACAGGTCGGCCAGGCCGACGGTGGTGGTGGGCTACGACG
CCGCCAACGGCAACGTCATGCTGAAGATCAGCAACAAGGGCGGCAAGGCCGTGAAGCTGTCCGTCACGGATAACGCCTAC
GGGGCGCAGACGCGCCGCTTCTCGATCCCGTCGGAAGGATTCGTCGAGGAAGTCTGGACGCTCAGGCACAGCCACCAATG
GTACGACCTGACGGTGTCCGACGGCGCGGCCTTCGCCTGGCGCGCCGCCGGCCACGTGGAGAACGGCAGGAGCAGCTTCA
GCGATCCGGCCGCCACGCAGGTGGTGACCGAGCTGTCGAGCTCGATCGTCACCTCGCCCACGGCGATCGCGCTGCAGGAC
CCGGCCCTGCCGGACATGCCGACGGTCTGA

Upstream 100 bases:

>100_bases
CGGCGGATTCCAGCAACTGACCGGCAAGCAGTCCTCGGCAGTCCGCCAATAGAAAGCCGCCCACCCACGTTTCACCGTAT
CTCTCACGAGAGGCCCGACC

Downstream 100 bases:

>100_bases
GCGGCCGCGTCCCGCCGCAAGGGGGCTGGGAGGCCGGCGACTCGCGCCTGCCGGCCCCGCACGCGGGCCCGGGCTGTTTG
CCGGCCGAATCCGGCGCCAT

Product: Non-hemolytic phospholipase C

Products: NA

Alternate protein names: PLC-N; Phosphatidylcholine cholinephosphohydrolase; Phosphatidylcholine-hydrolyzing phospholipase C; PC-PLC [H]

Number of amino acids: Translated: 729; Mature: 728

Protein sequence:

>729_residues
MSTYNRRRFLQTAASTAGAAAALTVFPDAIRKALAIPAASGTGSIRDVQHIVVLMQENRSFDHYFGHLRGVRGYNDRFPI
PLASGRPVWFQPSKADPATPVLPFRYDVNKVSECLGDLDHSWYPTHHAINNGRMDAWPANKSDLTMGYHVREDIPFHYAL
ADAFTICDHYFCSMPGPTHPNRTYLMTGMVDPTGKQGGPLLDNNDAIDDDLPPKWDLLTWTTYPERLQAAGISWQLYQQG
VNGNDPVNGNYGTNMLPNFANFINAPAGSALQTRGNAVRTLADLKNDVLANQLPQVCWLCPPAIYSEHPRYTPAYGATYI
AQILDALTSNPAVWSKTVFFLMYDENDGFFDHLAPPQPPTHRGQGLSTVDVSAEIHNVVNPRRGGSYTADNLPYGMGPRV
PMIVISPWTKGGYVNSQVFDHTSVIRFMEQRFGVMEPNISPWRRAVSGDLLSCFDFGTPDAAFPVLPDTSNYQAKSDASC
QNPKAAVPAVPSPASIKEQEAGVRAARALPYELHANGREAIKENEFQIHFSNTGKVGACFYVYSTNRSDGPWRYTVEAGK
SIADTFNLAGTGGVYNFLVYGPNGFVRHFVGAIPQDQKTRHRSARPTVVVGYDAANGNVMLKISNKGGKAVKLSVTDNAY
GAQTRRFSIPSEGFVEEVWTLRHSHQWYDLTVSDGAAFAWRAAGHVENGRSSFSDPAATQVVTELSSSIVTSPTAIALQD
PALPDMPTV

Sequences:

>Translated_729_residues
MSTYNRRRFLQTAASTAGAAAALTVFPDAIRKALAIPAASGTGSIRDVQHIVVLMQENRSFDHYFGHLRGVRGYNDRFPI
PLASGRPVWFQPSKADPATPVLPFRYDVNKVSECLGDLDHSWYPTHHAINNGRMDAWPANKSDLTMGYHVREDIPFHYAL
ADAFTICDHYFCSMPGPTHPNRTYLMTGMVDPTGKQGGPLLDNNDAIDDDLPPKWDLLTWTTYPERLQAAGISWQLYQQG
VNGNDPVNGNYGTNMLPNFANFINAPAGSALQTRGNAVRTLADLKNDVLANQLPQVCWLCPPAIYSEHPRYTPAYGATYI
AQILDALTSNPAVWSKTVFFLMYDENDGFFDHLAPPQPPTHRGQGLSTVDVSAEIHNVVNPRRGGSYTADNLPYGMGPRV
PMIVISPWTKGGYVNSQVFDHTSVIRFMEQRFGVMEPNISPWRRAVSGDLLSCFDFGTPDAAFPVLPDTSNYQAKSDASC
QNPKAAVPAVPSPASIKEQEAGVRAARALPYELHANGREAIKENEFQIHFSNTGKVGACFYVYSTNRSDGPWRYTVEAGK
SIADTFNLAGTGGVYNFLVYGPNGFVRHFVGAIPQDQKTRHRSARPTVVVGYDAANGNVMLKISNKGGKAVKLSVTDNAY
GAQTRRFSIPSEGFVEEVWTLRHSHQWYDLTVSDGAAFAWRAAGHVENGRSSFSDPAATQVVTELSSSIVTSPTAIALQD
PALPDMPTV
>Mature_728_residues
STYNRRRFLQTAASTAGAAAALTVFPDAIRKALAIPAASGTGSIRDVQHIVVLMQENRSFDHYFGHLRGVRGYNDRFPIP
LASGRPVWFQPSKADPATPVLPFRYDVNKVSECLGDLDHSWYPTHHAINNGRMDAWPANKSDLTMGYHVREDIPFHYALA
DAFTICDHYFCSMPGPTHPNRTYLMTGMVDPTGKQGGPLLDNNDAIDDDLPPKWDLLTWTTYPERLQAAGISWQLYQQGV
NGNDPVNGNYGTNMLPNFANFINAPAGSALQTRGNAVRTLADLKNDVLANQLPQVCWLCPPAIYSEHPRYTPAYGATYIA
QILDALTSNPAVWSKTVFFLMYDENDGFFDHLAPPQPPTHRGQGLSTVDVSAEIHNVVNPRRGGSYTADNLPYGMGPRVP
MIVISPWTKGGYVNSQVFDHTSVIRFMEQRFGVMEPNISPWRRAVSGDLLSCFDFGTPDAAFPVLPDTSNYQAKSDASCQ
NPKAAVPAVPSPASIKEQEAGVRAARALPYELHANGREAIKENEFQIHFSNTGKVGACFYVYSTNRSDGPWRYTVEAGKS
IADTFNLAGTGGVYNFLVYGPNGFVRHFVGAIPQDQKTRHRSARPTVVVGYDAANGNVMLKISNKGGKAVKLSVTDNAYG
AQTRRFSIPSEGFVEEVWTLRHSHQWYDLTVSDGAAFAWRAAGHVENGRSSFSDPAATQVVTELSSSIVTSPTAIALQDP
ALPDMPTV

Specific function: Hydrolyzes phosphatidylserine as well as phosphatidylcholine [H]

COG id: COG3511

COG function: function code M; Phospholipase C

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial phospholipase C family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007312
- InterPro:   IPR008475
- InterPro:   IPR017767
- InterPro:   IPR006311 [H]

Pfam domain/function: PF05506 DUF756; PF04185 Phosphoesterase [H]

EC number: =3.1.4.3 [H]

Molecular weight: Translated: 79928; Mature: 79797

Theoretical pI: Translated: 6.91; Mature: 6.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTYNRRRFLQTAASTAGAAAALTVFPDAIRKALAIPAASGTGSIRDVQHIVVLMQENRS
CCCHHHHHHHHHHHHCCCCCEEEHHCHHHHHHHHCCCCCCCCCCHHHHEEEEEEEECCCC
FDHYFGHLRGVRGYNDRFPIPLASGRPVWFQPSKADPATPVLPFRYDVNKVSECLGDLDH
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECHHHHHHHHHHCCC
SWYPTHHAINNGRMDAWPANKSDLTMGYHVREDIPFHYALADAFTICDHYFCSMPGPTHP
CCCCCHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCCCC
NRTYLMTGMVDPTGKQGGPLLDNNDAIDDDLPPKWDLLTWTTYPERLQAAGISWQLYQQG
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHCCCEEEHHHCC
VNGNDPVNGNYGTNMLPNFANFINAPAGSALQTRGNAVRTLADLKNDVLANQLPQVCWLC
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
PPAIYSEHPRYTPAYGATYIAQILDALTSNPAVWSKTVFFLMYDENDGFFDHLAPPQPPT
CHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCHHCCCCCCCCC
HRGQGLSTVDVSAEIHNVVNPRRGGSYTADNLPYGMGPRVPMIVISPWTKGGYVNSQVFD
CCCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHH
HTSVIRFMEQRFGVMEPNISPWRRAVSGDLLSCFDFGTPDAAFPVLPDTSNYQAKSDASC
HHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
QNPKAAVPAVPSPASIKEQEAGVRAARALPYELHANGREAIKENEFQIHFSNTGKVGACF
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHCCCCEEEEECCCCCEEEEE
YVYSTNRSDGPWRYTVEAGKSIADTFNLAGTGGVYNFLVYGPNGFVRHFVGAIPQDQKTR
EEEECCCCCCCEEEEECCCCHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHCCCCCHHHH
HRSARPTVVVGYDAANGNVMLKISNKGGKAVKLSVTDNAYGAQTRRFSIPSEGFVEEVWT
HCCCCCEEEEEEECCCCEEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCHHHHHHHH
LRHSHQWYDLTVSDGAAFAWRAAGHVENGRSSFSDPAATQVVTELSSSIVTSPTAIALQD
HHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEC
PALPDMPTV
CCCCCCCCC
>Mature Secondary Structure 
STYNRRRFLQTAASTAGAAAALTVFPDAIRKALAIPAASGTGSIRDVQHIVVLMQENRS
CCHHHHHHHHHHHHCCCCCEEEHHCHHHHHHHHCCCCCCCCCCHHHHEEEEEEEECCCC
FDHYFGHLRGVRGYNDRFPIPLASGRPVWFQPSKADPATPVLPFRYDVNKVSECLGDLDH
HHHHHHHHCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEECHHHHHHHHHHCCC
SWYPTHHAINNGRMDAWPANKSDLTMGYHVREDIPFHYALADAFTICDHYFCSMPGPTHP
CCCCCHHHCCCCCCCCCCCCCCCEEEEEEECCCCCEEHHHHHHHHHHHHHHCCCCCCCCC
NRTYLMTGMVDPTGKQGGPLLDNNDAIDDDLPPKWDLLTWTTYPERLQAAGISWQLYQQG
CCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHCCCEEEHHHCC
VNGNDPVNGNYGTNMLPNFANFINAPAGSALQTRGNAVRTLADLKNDVLANQLPQVCWLC
CCCCCCCCCCCCCCCCCCHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCC
PPAIYSEHPRYTPAYGATYIAQILDALTSNPAVWSKTVFFLMYDENDGFFDHLAPPQPPT
CHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEEEEEECCCCCHHCCCCCCCCC
HRGQGLSTVDVSAEIHNVVNPRRGGSYTADNLPYGMGPRVPMIVISPWTKGGYVNSQVFD
CCCCCCEEEECCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCCHHHH
HTSVIRFMEQRFGVMEPNISPWRRAVSGDLLSCFDFGTPDAAFPVLPDTSNYQAKSDASC
HHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
QNPKAAVPAVPSPASIKEQEAGVRAARALPYELHANGREAIKENEFQIHFSNTGKVGACF
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCHHHHCCCCEEEEECCCCCEEEEE
YVYSTNRSDGPWRYTVEAGKSIADTFNLAGTGGVYNFLVYGPNGFVRHFVGAIPQDQKTR
EEEECCCCCCCEEEEECCCCHHHHHHCCCCCCCEEEEEEECCCHHHHHHHHCCCCCHHHH
HRSARPTVVVGYDAANGNVMLKISNKGGKAVKLSVTDNAYGAQTRRFSIPSEGFVEEVWT
HCCCCCEEEEEEECCCCEEEEEEECCCCCEEEEEECCCCCCCCEEEECCCCCHHHHHHHH
LRHSHQWYDLTVSDGAAFAWRAAGHVENGRSSFSDPAATQVVTELSSSIVTSPTAIALQD
HHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEC
PALPDMPTV
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10523590 [H]