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The map label for this gene is pglA [H]

Identifier: 238025222

GI number: 238025222

Start: 2360976

End: 2363129

Strand: Direct

Name: pglA [H]

Synonym: bglu_2g18930

Alternate gene names: 238025222

Gene position: 2360976-2363129 (Clockwise)

Preceding gene: 238025220

Following gene: 238025227

Centisome position: 83.51

GC content: 68.29

Gene sequence:

>2154_bases
ATGGGACCCTCATCACGGACGCGCGCGCCGCGCCTGCTGTGCCTGCTCGCCGCCGCGCTGGCGACGGCCGCCGCGCTCGG
TGCATGCGGCGGCGACGCCGACACGCCCGCCGTCGCCACCGGCACGGGCACGCCCTCCGTTGCCAATGGCGTGTTCCAGG
TCGGCACCACGACCTCGGATGCGAACCTGCCGGCCGAGCCGAGCCTGCCCACCGACGCGCAGGTCTGCAGCACGCTGGAG
GCGAGCAACACGCTGGTGAGCCGCCCGGACGGCTCCTTGCCGCCCGAGGCCGATCCTTCCGTGGCGGGCGTCGGCAAGGC
GGTGTCGCCCGCCACGGCCAACCCCGATCAGGCGCGCATCCAGGCCGCGCTCGACGCCTGCGGCGCCGCGGTGGACGCGC
AAGTGGGAGCGGCCATCGCGGCCGCAGACGCGTCGGCGACCGCCGCGCAAAAGGCCGCCGCCGTCGCGAACGTGAACATC
TCGGGCGCCTCCGGCGAGGAGCTGGCCAAGCCCGCCTACCGCGCCAGCAAGTTCGCCGTGCGCCTGGTGGTCAGCAGCAG
CGGCGCCGGCAACGGCTTCATCAGCGGCCCGCTGACGCTGCCCTCGGGCGTGACGCTGTGGATCGACAAGGGCGTGACGC
TGTACGCCTCGCGCGACGTGAAGGCCTATGCGCCGACGGCCGCCGGCCCCTACTGCGCCAACACCGCCGTCAGCGCCACC
AAGGCCGGCAGTTCGTCGAACTGCCTGGCGCTCATCACCGGCACCAACCTGGTCAACTCGGCGGTGGTCGGCGACGGCCG
CATCGACGGGCGCGGCTACGCGGAGATCGTCACGTCCGATGCGAAATACCCGCTGATGAAGGTGGACCTCACCTGCTCGA
ACACCTATGCCGCCTACAAGACCGGCACGGTGGCGCCCGACGGCACGCCCTGCGATGACGGCGGCACGGTGGTGGACTCG
AAATCCTCGGTGCGCAACATGAGCTGGTGGGATCTGGCCTACCTCGGCAACATGGTGCAGAACGGCACCACCGGCACGGC
GTCGCAGTCCAACTTCCGGCTAATGGTGTTCAACTACGCGAAGAACCTGACGCTGTATCGCATCACGCTCAACAACAGCC
CGAACTTCCACGTGGTGCCGAGCGGCGTCGACGGCCTGACGGTCTGGGGCGTGAAGGTGCAAACGCCGACCCTGGCCGCG
TTCGCGAACCCGGCCGGCAACGGCAATCCGCTCTATACCGGCCAGACCTTCGGCGAGGACAACGTCAAGAACACCGACGC
CTTCGATCCCGGCTCGGCCTCGAAGGCCACCTCGGCGGCCCTTTCGACGGGCAGCACGACGAACTCCGCCGCCGGCGCGA
TCTCGTTCGACGGCTACCTGAAGAACTTCGTGTTCGCCTACAACTACGTGAGCACCGGCGACGACGACATCGCCCTCAAG
GGCAGCAGCAACCCGTCGCCGGCCGGCTCGGGCCTGTTCGGCGTGGACGGCAACCGCAACGTGCGCGCGGATCGCAAATG
GGGCATCGTGATCGCCCATAACCACATCTACTGGGGGCACGGCATCTCGATCGGCAGCGAGACCAACGCGGGCGTGACGA
ACGTGCAGGTCTACGACAATTCGTTCTGGGATTCGGAGGAAGGGCTGCGCATCAAGTCCGACTATGCGCGCGGCGGCGAG
GTGAGCAACATCCACTACGCGAACATCTGCATTCGCGACGCCACCAACGCGCTGCTCTTCACGCCGTACTACAGCACGAA
GGCGCTGCCGGCCAGCGGCCCGCTGTACCCGAATTTCCACGACATCTCGATCTCGAACGTGGTGATCACGGGCTCGGCCG
GCGTCAAGCTGCAAGGCTTCGAGGCGAACACCGGCGGCTTCGGCGAGCCGGCCTTCCCGCTCGTGATGACGCTGACCAAC
GTGCTGGCCGATTCGCCGGACAACATCTCGGTGATCTCGTCCGATGCCAACCTGACGCTGCAGAACGTGAACCTGCCGAT
CTTCGCCTCGGCCGCGAACCGCGTCGTGCTGAACGGCGCCGCCAACCACGCCCTGACCAGCAGCGGCGTGCTCGATTGCA
GCAAGGCGTTCGTCGACTTCCCGGGCATCGATCAGTCGAATCCGTTCGGCACCACCTGGGCGCCTTCCTTGTAG

Upstream 100 bases:

>100_bases
ATCCTGACAAGCCGGCGGCGCGATCGCACGGCATGTCGTCAGCAAATCCATACCAACCCGGCATCGTCATCGCGCGATGC
CCAGACAGGAGACAGCTTCC

Downstream 100 bases:

>100_bases
GCGCGAGGCGAGGCGGCATGCCCGCGCCCCGCGCGGGCCGCTCAGCCCGGCACCGGCGCCAGGAACCGCTCCACCAGCCG
CACGAAATAGCTGGCGCCCA

Product: glycoside hydrolase

Products: NA

Alternate protein names: PGA; Pectinase [H]

Number of amino acids: Translated: 717; Mature: 716

Protein sequence:

>717_residues
MGPSSRTRAPRLLCLLAAALATAAALGACGGDADTPAVATGTGTPSVANGVFQVGTTTSDANLPAEPSLPTDAQVCSTLE
ASNTLVSRPDGSLPPEADPSVAGVGKAVSPATANPDQARIQAALDACGAAVDAQVGAAIAAADASATAAQKAAAVANVNI
SGASGEELAKPAYRASKFAVRLVVSSSGAGNGFISGPLTLPSGVTLWIDKGVTLYASRDVKAYAPTAAGPYCANTAVSAT
KAGSSSNCLALITGTNLVNSAVVGDGRIDGRGYAEIVTSDAKYPLMKVDLTCSNTYAAYKTGTVAPDGTPCDDGGTVVDS
KSSVRNMSWWDLAYLGNMVQNGTTGTASQSNFRLMVFNYAKNLTLYRITLNNSPNFHVVPSGVDGLTVWGVKVQTPTLAA
FANPAGNGNPLYTGQTFGEDNVKNTDAFDPGSASKATSAALSTGSTTNSAAGAISFDGYLKNFVFAYNYVSTGDDDIALK
GSSNPSPAGSGLFGVDGNRNVRADRKWGIVIAHNHIYWGHGISIGSETNAGVTNVQVYDNSFWDSEEGLRIKSDYARGGE
VSNIHYANICIRDATNALLFTPYYSTKALPASGPLYPNFHDISISNVVITGSAGVKLQGFEANTGGFGEPAFPLVMTLTN
VLADSPDNISVISSDANLTLQNVNLPIFASAANRVVLNGAANHALTSSGVLDCSKAFVDFPGIDQSNPFGTTWAPSL

Sequences:

>Translated_717_residues
MGPSSRTRAPRLLCLLAAALATAAALGACGGDADTPAVATGTGTPSVANGVFQVGTTTSDANLPAEPSLPTDAQVCSTLE
ASNTLVSRPDGSLPPEADPSVAGVGKAVSPATANPDQARIQAALDACGAAVDAQVGAAIAAADASATAAQKAAAVANVNI
SGASGEELAKPAYRASKFAVRLVVSSSGAGNGFISGPLTLPSGVTLWIDKGVTLYASRDVKAYAPTAAGPYCANTAVSAT
KAGSSSNCLALITGTNLVNSAVVGDGRIDGRGYAEIVTSDAKYPLMKVDLTCSNTYAAYKTGTVAPDGTPCDDGGTVVDS
KSSVRNMSWWDLAYLGNMVQNGTTGTASQSNFRLMVFNYAKNLTLYRITLNNSPNFHVVPSGVDGLTVWGVKVQTPTLAA
FANPAGNGNPLYTGQTFGEDNVKNTDAFDPGSASKATSAALSTGSTTNSAAGAISFDGYLKNFVFAYNYVSTGDDDIALK
GSSNPSPAGSGLFGVDGNRNVRADRKWGIVIAHNHIYWGHGISIGSETNAGVTNVQVYDNSFWDSEEGLRIKSDYARGGE
VSNIHYANICIRDATNALLFTPYYSTKALPASGPLYPNFHDISISNVVITGSAGVKLQGFEANTGGFGEPAFPLVMTLTN
VLADSPDNISVISSDANLTLQNVNLPIFASAANRVVLNGAANHALTSSGVLDCSKAFVDFPGIDQSNPFGTTWAPSL
>Mature_716_residues
GPSSRTRAPRLLCLLAAALATAAALGACGGDADTPAVATGTGTPSVANGVFQVGTTTSDANLPAEPSLPTDAQVCSTLEA
SNTLVSRPDGSLPPEADPSVAGVGKAVSPATANPDQARIQAALDACGAAVDAQVGAAIAAADASATAAQKAAAVANVNIS
GASGEELAKPAYRASKFAVRLVVSSSGAGNGFISGPLTLPSGVTLWIDKGVTLYASRDVKAYAPTAAGPYCANTAVSATK
AGSSSNCLALITGTNLVNSAVVGDGRIDGRGYAEIVTSDAKYPLMKVDLTCSNTYAAYKTGTVAPDGTPCDDGGTVVDSK
SSVRNMSWWDLAYLGNMVQNGTTGTASQSNFRLMVFNYAKNLTLYRITLNNSPNFHVVPSGVDGLTVWGVKVQTPTLAAF
ANPAGNGNPLYTGQTFGEDNVKNTDAFDPGSASKATSAALSTGSTTNSAAGAISFDGYLKNFVFAYNYVSTGDDDIALKG
SSNPSPAGSGLFGVDGNRNVRADRKWGIVIAHNHIYWGHGISIGSETNAGVTNVQVYDNSFWDSEEGLRIKSDYARGGEV
SNIHYANICIRDATNALLFTPYYSTKALPASGPLYPNFHDISISNVVITGSAGVKLQGFEANTGGFGEPAFPLVMTLTNV
LADSPDNISVISSDANLTLQNVNLPIFASAANRVVLNGAANHALTSSGVLDCSKAFVDFPGIDQSNPFGTTWAPSL

Specific function: Contributes to the wilt disease production on tomato [H]

COG id: COG5434

COG function: function code M; Endopolygalacturonase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 28 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000743
- InterPro:   IPR012334
- InterPro:   IPR011050 [H]

Pfam domain/function: PF00295 Glyco_hydro_28 [H]

EC number: =3.2.1.15 [H]

Molecular weight: Translated: 73220; Mature: 73088

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00306 CASEIN_ALPHA_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPSSRTRAPRLLCLLAAALATAAALGACGGDADTPAVATGTGTPSVANGVFQVGTTTSD
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHCCEEEECCCCCC
ANLPAEPSLPTDAQVCSTLEASNTLVSRPDGSLPPEADPSVAGVGKAVSPATANPDQARI
CCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHH
QAALDACGAAVDAQVGAAIAAADASATAAQKAAAVANVNISGASGEELAKPAYRASKFAV
HHHHHHHCCHHHHHHCCEEEECCCCHHHHHHHEEEEEEEECCCCCHHHHHHHHHCCEEEE
RLVVSSSGAGNGFISGPLTLPSGVTLWIDKGVTLYASRDVKAYAPTAAGPYCANTAVSAT
EEEEECCCCCCCEEECCEECCCCEEEEEECCEEEEEECCCEEECCCCCCCCCCCCCCCCC
KAGSSSNCLALITGTNLVNSAVVGDGRIDGRGYAEIVTSDAKYPLMKVDLTCSNTYAAYK
CCCCCCCEEEEEECCCCCCCEEECCCCCCCCCEEEEEECCCCCCEEEEEEEECCCEEEEE
TGTVAPDGTPCDDGGTVVDSKSSVRNMSWWDLAYLGNMVQNGTTGTASQSNFRLMVFNYA
CCCCCCCCCCCCCCCEEEECCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEC
KNLTLYRITLNNSPNFHVVPSGVDGLTVWGVKVQTPTLAAFANPAGNGNPLYTGQTFGED
CCEEEEEEEECCCCCEEEEECCCCCEEEEEEEECCCEEEEECCCCCCCCEEECCCCCCCC
NVKNTDAFDPGSASKATSAALSTGSTTNSAAGAISFDGYLKNFVFAYNYVSTGDDDIALK
CCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEHHHHHCEEEEEEEECCCCCCEEEE
GSSNPSPAGSGLFGVDGNRNVRADRKWGIVIAHNHIYWGHGISIGSETNAGVTNVQVYDN
CCCCCCCCCCCEEECCCCCCCCCCCCEEEEEEECEEEEECCEECCCCCCCCEEEEEEECC
SFWDSEEGLRIKSDYARGGEVSNIHYANICIRDATNALLFTPYYSTKALPASGPLYPNFH
CCCCCCCCCEEEECCCCCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEE
DISISNVVITGSAGVKLQGFEANTGGFGEPAFPLVMTLTNVLADSPDNISVISSDANLTL
EEEEEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEE
QNVNLPIFASAANRVVLNGAANHALTSSGVLDCSKAFVDFPGIDQSNPFGTTWAPSL
EECCCEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GPSSRTRAPRLLCLLAAALATAAALGACGGDADTPAVATGTGTPSVANGVFQVGTTTSD
CCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHCCEEEECCCCCC
ANLPAEPSLPTDAQVCSTLEASNTLVSRPDGSLPPEADPSVAGVGKAVSPATANPDQARI
CCCCCCCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCHHHHH
QAALDACGAAVDAQVGAAIAAADASATAAQKAAAVANVNISGASGEELAKPAYRASKFAV
HHHHHHHCCHHHHHHCCEEEECCCCHHHHHHHEEEEEEEECCCCCHHHHHHHHHCCEEEE
RLVVSSSGAGNGFISGPLTLPSGVTLWIDKGVTLYASRDVKAYAPTAAGPYCANTAVSAT
EEEEECCCCCCCEEECCEECCCCEEEEEECCEEEEEECCCEEECCCCCCCCCCCCCCCCC
KAGSSSNCLALITGTNLVNSAVVGDGRIDGRGYAEIVTSDAKYPLMKVDLTCSNTYAAYK
CCCCCCCEEEEEECCCCCCCEEECCCCCCCCCEEEEEECCCCCCEEEEEEEECCCEEEEE
TGTVAPDGTPCDDGGTVVDSKSSVRNMSWWDLAYLGNMVQNGTTGTASQSNFRLMVFNYA
CCCCCCCCCCCCCCCEEEECCHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEC
KNLTLYRITLNNSPNFHVVPSGVDGLTVWGVKVQTPTLAAFANPAGNGNPLYTGQTFGED
CCEEEEEEEECCCCCEEEEECCCCCEEEEEEEECCCEEEEECCCCCCCCEEECCCCCCCC
NVKNTDAFDPGSASKATSAALSTGSTTNSAAGAISFDGYLKNFVFAYNYVSTGDDDIALK
CCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCEEEEHHHHHCEEEEEEEECCCCCCEEEE
GSSNPSPAGSGLFGVDGNRNVRADRKWGIVIAHNHIYWGHGISIGSETNAGVTNVQVYDN
CCCCCCCCCCCEEECCCCCCCCCCCCEEEEEEECEEEEECCEECCCCCCCCEEEEEEECC
SFWDSEEGLRIKSDYARGGEVSNIHYANICIRDATNALLFTPYYSTKALPASGPLYPNFH
CCCCCCCCCEEEECCCCCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCCCCCCCCCCEE
DISISNVVITGSAGVKLQGFEANTGGFGEPAFPLVMTLTNVLADSPDNISVISSDANLTL
EEEEEEEEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCEEE
QNVNLPIFASAANRVVLNGAANHALTSSGVLDCSKAFVDFPGIDQSNPFGTTWAPSL
EECCCEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2193922 [H]