Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 238025210

GI number: 238025210

Start: 2345572

End: 2346519

Strand: Direct

Name: gcvA [H]

Synonym: bglu_2g18810

Alternate gene names: 238025210

Gene position: 2345572-2346519 (Clockwise)

Preceding gene: 238025209

Following gene: 238025212

Centisome position: 82.96

GC content: 67.3

Gene sequence:

>948_bases
ATGTCGAGTCTGGACACGAACCGATACTCCGCACTGCCTTCGTTACGTGCGCTGCGCTACTTCGTCAGCGTCGGGCGGCA
CCGCAGCATCAAGATCGCCGCGGAGGAAATCCACGTCACGCCGTCGGCGATCAGCCAGCAGATCGTCAAGCTCGAGGAAG
AACTCGGCGTGAGCCTGCTGCAGCGCTCGCCGCGCGGCATCGACATCACGCCGCACGGCAGCCGCTACCTGCAGGAACTG
CAGCAGGTGTTCGACCTGATCCTGCGCGCGACCGACCGCCTGCGCAGCAACGCGAACCACACCGACATCGTCACCGTCAG
TTGCGCGCACAGCTTCGCGATGCAATGGCTGGTGCCGCGCCTGTCCGATCTCGCGCAGTACGCGCCGTCCGTCGACCTGC
GCATCAGCACGACCAACCGCTATGCCGCCTTCATCGACGACCAGATCGATTTCGGGCTGCGCCACGGCACCGGCCGCTAC
CCGGGGCTCAAGTCCGAACTGCTGCTCGACGATCCGCTCTATCCGATCTGCAGCCCCCGGCTGGTGGAACGGCTCGGGCC
GTTCGCGTCGTACCAGGACCTCGCGCGCACCACGCTGCTGCACGACGAGCGCCATAAGGAATGGCTCGGCTGGCTCAGGC
ATCACGGAGTGGAGGGCGTGCGCGGCGACAAGGGCGCCGTGTTCATCGATTCGAACGGCATGCTGCAGGCCGCGGTCGCC
TGCCACGGCGTGGCGCTCGCGCGCCGCTCGATCGTCGCCTCGGAGCTGGCCGACGGACGCCTGACGGTGCTGTTCGACGC
GCCGTTCACTCCCGATTACGCCTACTACCTCGTCTATCCGGAGCAGACGCTCGACAATCCGAGCGCACGCGTGTTCCGCG
AGTGGCTGATGCAAGCGGTCAAGGGCTCCGGCGTGCGCGGCGCAGCGGCCGACCTCGCACCGGACTGA

Upstream 100 bases:

>100_bases
CTCCGTCCCGGCCGGACAGCGCGGCTGGCCCCTATCCGCGTCCGGAGCGGCTTGCCTGCGGCGGCGCGACCGCTCAACTT
TCAGGCAAAGTCAGACAAAC

Downstream 100 bases:

>100_bases
TCGACGGCAGCGGCCGCCGCCCGGCCGCGCCGCGCTCTTGCTTCACACGCTCTTGCTTCATATCGCGCCGGCCGCCACGC
CGCGGCCCGCCACGCCGGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 315; Mature: 314

Protein sequence:

>315_residues
MSSLDTNRYSALPSLRALRYFVSVGRHRSIKIAAEEIHVTPSAISQQIVKLEEELGVSLLQRSPRGIDITPHGSRYLQEL
QQVFDLILRATDRLRSNANHTDIVTVSCAHSFAMQWLVPRLSDLAQYAPSVDLRISTTNRYAAFIDDQIDFGLRHGTGRY
PGLKSELLLDDPLYPICSPRLVERLGPFASYQDLARTTLLHDERHKEWLGWLRHHGVEGVRGDKGAVFIDSNGMLQAAVA
CHGVALARRSIVASELADGRLTVLFDAPFTPDYAYYLVYPEQTLDNPSARVFREWLMQAVKGSGVRGAAADLAPD

Sequences:

>Translated_315_residues
MSSLDTNRYSALPSLRALRYFVSVGRHRSIKIAAEEIHVTPSAISQQIVKLEEELGVSLLQRSPRGIDITPHGSRYLQEL
QQVFDLILRATDRLRSNANHTDIVTVSCAHSFAMQWLVPRLSDLAQYAPSVDLRISTTNRYAAFIDDQIDFGLRHGTGRY
PGLKSELLLDDPLYPICSPRLVERLGPFASYQDLARTTLLHDERHKEWLGWLRHHGVEGVRGDKGAVFIDSNGMLQAAVA
CHGVALARRSIVASELADGRLTVLFDAPFTPDYAYYLVYPEQTLDNPSARVFREWLMQAVKGSGVRGAAADLAPD
>Mature_314_residues
SSLDTNRYSALPSLRALRYFVSVGRHRSIKIAAEEIHVTPSAISQQIVKLEEELGVSLLQRSPRGIDITPHGSRYLQELQ
QVFDLILRATDRLRSNANHTDIVTVSCAHSFAMQWLVPRLSDLAQYAPSVDLRISTTNRYAAFIDDQIDFGLRHGTGRYP
GLKSELLLDDPLYPICSPRLVERLGPFASYQDLARTTLLHDERHKEWLGWLRHHGVEGVRGDKGAVFIDSNGMLQAAVAC
HGVALARRSIVASELADGRLTVLFDAPFTPDYAYYLVYPEQTLDNPSARVFREWLMQAVKGSGVRGAAADLAPD

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=286, Percent_Identity=36.3636363636364, Blast_Score=191, Evalue=8e-50,
Organism=Escherichia coli, GI1786448, Length=295, Percent_Identity=32.8813559322034, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1788706, Length=297, Percent_Identity=29.2929292929293, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1786401, Length=303, Percent_Identity=24.7524752475248, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI145693193, Length=293, Percent_Identity=24.2320819112628, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081978, Length=299, Percent_Identity=26.0869565217391, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI157672245, Length=199, Percent_Identity=29.6482412060301, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI145693105, Length=180, Percent_Identity=24.4444444444444, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787879, Length=300, Percent_Identity=23, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1789440, Length=296, Percent_Identity=22.972972972973, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 35150; Mature: 35019

Theoretical pI: Translated: 7.12; Mature: 7.12

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLDTNRYSALPSLRALRYFVSVGRHRSIKIAAEEIHVTPSAISQQIVKLEEELGVSLL
CCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHEECCHHHHHHHHHHHHHHHCHHHH
QRSPRGIDITPHGSRYLQELQQVFDLILRATDRLRSNANHTDIVTVSCAHSFAMQWLVPR
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHH
LSDLAQYAPSVDLRISTTNRYAAFIDDQIDFGLRHGTGRYPGLKSELLLDDPLYPICSPR
HHHHHHHCCCCEEEEECCCCEEEEECCHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCHH
LVERLGPFASYQDLARTTLLHDERHKEWLGWLRHHGVEGVRGDKGAVFIDSNGMLQAAVA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHH
CHGVALARRSIVASELADGRLTVLFDAPFTPDYAYYLVYPEQTLDNPSARVFREWLMQAV
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECHHHCCCHHHHHHHHHHHHHH
KGSGVRGAAADLAPD
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSLDTNRYSALPSLRALRYFVSVGRHRSIKIAAEEIHVTPSAISQQIVKLEEELGVSLL
CCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEHHHEECCHHHHHHHHHHHHHHHCHHHH
QRSPRGIDITPHGSRYLQELQQVFDLILRATDRLRSNANHTDIVTVSCAHSFAMQWLVPR
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHHH
LSDLAQYAPSVDLRISTTNRYAAFIDDQIDFGLRHGTGRYPGLKSELLLDDPLYPICSPR
HHHHHHHCCCCEEEEECCCCEEEEECCHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCHH
LVERLGPFASYQDLARTTLLHDERHKEWLGWLRHHGVEGVRGDKGAVFIDSNGMLQAAVA
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHH
CHGVALARRSIVASELADGRLTVLFDAPFTPDYAYYLVYPEQTLDNPSARVFREWLMQAV
HHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEEECHHHCCCHHHHHHHHHHHHHH
KGSGVRGAAADLAPD
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]