Click here to switch to the map view.

The map label for this gene is ntaA [H]

Identifier: 238024696

GI number: 238024696

Start: 1709630

End: 1711039

Strand: Reverse

Name: ntaA [H]

Synonym: bglu_2g13330

Alternate gene names: 238024696

Gene position: 1711039-1709630 (Counterclockwise)

Preceding gene: 238024697

Following gene: 238024693

Centisome position: 60.52

GC content: 68.09

Gene sequence:

>1410_bases
ATGAGCCACCCCATCCGCTTCAATGCGTTCGAGATGAATTGCGTCGGACATCAGTCGCCGGGGCTCTGGTCGCATCCGCG
CGATCGCTCGTGGCAGTACAAGGACCTCGAATACTGGACCGATCTCGCGAAGACGCTCGAGAAGGGAATCTTCGACGGCA
TCTTCATCGCCGACGTGATCGGCTACTACGACGTCTATCACGGCAGCAGCCACCATGCCCTGCATCAGGGCGTGCAGATT
CCCGTCAACGATCCGCTGCAGCTCGCCGCGCCGATCGCGCTCGCCACCGAGCACCTCGGCATCGGCATCACCGCCTCCAC
CTCCTTCGAGCACCCGTACACGTTCGCGCGCCGGCTTTCCACCGCCGATCACCACACGAAGGGCCGGGTCGGCTGGAACA
TCGTCACGTCGTATCTGGAGAGCGGCGCGCGCAATGTCGGCCAGGCCGGGCTCGCGGATCACACCAACCGCTATGAGATC
GCCGCCGAGTATCTGGAGGTGCTGTACAAGCTGTTCGAGGGGAGCTGGGAGGACGGCGCCGTGCTGCGCGACCGCGAGCG
CAGGATCTTCGCCGATCCTGCGAAAATCCACGAGATCCGCCACCACGGCAGGCACTTCGAGGTGCCCGGCTACCACCTCT
GCGAGCCGTCGCCGCAGCGCACGCCGGTGCTGTATCAGGCCGGTGCCTCCGGGGCCGGCAAGGCGTTCGCGGCGCAGCAC
GCGGAGTGCGTGTTCGTCGCCGCGCCCACGCGCGCCACGCTGCGCGACTACGTGGCCGACATCCGCCGGCGCGCGGCAGA
GGCCGGGCGCGACGCGCACAAGCTGCTGATCTACACGCTGGTGACGATCGTGGTCGATGAAACCGACCAGAAAGCGCAGG
CCAAGTTCGACGACTACCTGCGGCACGTGTCCTACGATGGCTCGCTGGTGCTGATGTCGGGCTGGACCGGCATCGACTTC
GGCCAGTACGCACCGACCGATCTGGTGCGCCATGTCGAGACCAACGCGATCGTGTCGGCCGTCGAGCACCTGGCCGGCGG
CGACCGGCGCTGGACCATCGACGAGCTGGCGCGTTTCGGCGGCGTGGGCGGCCTGGGTCCGGTGTTCGTCGGCTCGCCCG
CGACGGTCGCGGACATTCTGCAGGAATGGCGCGAACACACCGGCGTGGACGGCTTCAATCTCGCCTACGCGCTCGCGCAC
GAGACCTTCGAGGACGTCGTCGAATATCTGGTGCCCGAATTGCAGCGGCGCGGCGCCTATCCGACCGCCTACCGGCCCGG
CACGCTGCGCGAAAAGCTGTTCGGCGAGGGACCGCGCCTGGGCGGGACCCATCCCGGCGCCGCGTATCGCGACTTGTCGC
GCTTCACGCGCGGCGCGCCGACCCCGGCGGCGGAAGCCACGCCCGCCTGA

Upstream 100 bases:

>100_bases
GGTTCGATAGATTCGGCGGATGGTCCGGCTTACGGCGGGCAGCGGCCCCCATGAGCCGGTTGCGCATCGCGCGGCCCTTT
TTCGACCGGAGATATCGAGC

Downstream 100 bases:

>100_bases
CCCGCATCGGGCCGCTGCCCGCGCCGCGCGGGTAACTGCACGGCGATGAAAAACCCGGCCCACGACGACATGCCATGGGC
CGGGCGGGATGGTGCCGGCA

Product: Flavin-dependent oxidoreductase-like protein

Products: an aldehyde; SO3; H2O; FMN [C]

Alternate protein names: NTA monooxygenase component A; NTA-MO A [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSHPIRFNAFEMNCVGHQSPGLWSHPRDRSWQYKDLEYWTDLAKTLEKGIFDGIFIADVIGYYDVYHGSSHHALHQGVQI
PVNDPLQLAAPIALATEHLGIGITASTSFEHPYTFARRLSTADHHTKGRVGWNIVTSYLESGARNVGQAGLADHTNRYEI
AAEYLEVLYKLFEGSWEDGAVLRDRERRIFADPAKIHEIRHHGRHFEVPGYHLCEPSPQRTPVLYQAGASGAGKAFAAQH
AECVFVAAPTRATLRDYVADIRRRAAEAGRDAHKLLIYTLVTIVVDETDQKAQAKFDDYLRHVSYDGSLVLMSGWTGIDF
GQYAPTDLVRHVETNAIVSAVEHLAGGDRRWTIDELARFGGVGGLGPVFVGSPATVADILQEWREHTGVDGFNLAYALAH
ETFEDVVEYLVPELQRRGAYPTAYRPGTLREKLFGEGPRLGGTHPGAAYRDLSRFTRGAPTPAAEATPA

Sequences:

>Translated_469_residues
MSHPIRFNAFEMNCVGHQSPGLWSHPRDRSWQYKDLEYWTDLAKTLEKGIFDGIFIADVIGYYDVYHGSSHHALHQGVQI
PVNDPLQLAAPIALATEHLGIGITASTSFEHPYTFARRLSTADHHTKGRVGWNIVTSYLESGARNVGQAGLADHTNRYEI
AAEYLEVLYKLFEGSWEDGAVLRDRERRIFADPAKIHEIRHHGRHFEVPGYHLCEPSPQRTPVLYQAGASGAGKAFAAQH
AECVFVAAPTRATLRDYVADIRRRAAEAGRDAHKLLIYTLVTIVVDETDQKAQAKFDDYLRHVSYDGSLVLMSGWTGIDF
GQYAPTDLVRHVETNAIVSAVEHLAGGDRRWTIDELARFGGVGGLGPVFVGSPATVADILQEWREHTGVDGFNLAYALAH
ETFEDVVEYLVPELQRRGAYPTAYRPGTLREKLFGEGPRLGGTHPGAAYRDLSRFTRGAPTPAAEATPA
>Mature_468_residues
SHPIRFNAFEMNCVGHQSPGLWSHPRDRSWQYKDLEYWTDLAKTLEKGIFDGIFIADVIGYYDVYHGSSHHALHQGVQIP
VNDPLQLAAPIALATEHLGIGITASTSFEHPYTFARRLSTADHHTKGRVGWNIVTSYLESGARNVGQAGLADHTNRYEIA
AEYLEVLYKLFEGSWEDGAVLRDRERRIFADPAKIHEIRHHGRHFEVPGYHLCEPSPQRTPVLYQAGASGAGKAFAAQHA
ECVFVAAPTRATLRDYVADIRRRAAEAGRDAHKLLIYTLVTIVVDETDQKAQAKFDDYLRHVSYDGSLVLMSGWTGIDFG
QYAPTDLVRHVETNAIVSAVEHLAGGDRRWTIDELARFGGVGGLGPVFVGSPATVADILQEWREHTGVDGFNLAYALAHE
TFEDVVEYLVPELQRRGAYPTAYRPGTLREKLFGEGPRLGGTHPGAAYRDLSRFTRGAPTPAAEATPA

Specific function: Hydroxylation of nitrilotriacetate [H]

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ntaA/snaA/soxA(dszA) monooxygenase family [H]

Homologues:

Organism=Escherichia coli, GI1787166, Length=361, Percent_Identity=24.9307479224377, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011251
- InterPro:   IPR016048
- InterPro:   IPR016215 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: 1.14.14.5 [C]

Molecular weight: Translated: 51978; Mature: 51846

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHPIRFNAFEMNCVGHQSPGLWSHPRDRSWQYKDLEYWTDLAKTLEKGIFDGIFIADVI
CCCCCEECEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHH
GYYDVYHGSSHHALHQGVQIPVNDPLQLAAPIALATEHLGIGITASTSFEHPYTFARRLS
HHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHH
TADHHTKGRVGWNIVTSYLESGARNVGQAGLADHTNRYEIAAEYLEVLYKLFEGSWEDGA
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
VLRDRERRIFADPAKIHEIRHHGRHFEVPGYHLCEPSPQRTPVLYQAGASGAGKAFAAQH
HHHCCCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHCC
AECVFVAAPTRATLRDYVADIRRRAAEAGRDAHKLLIYTLVTIVVDETDQKAQAKFDDYL
CCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RHVSYDGSLVLMSGWTGIDFGQYAPTDLVRHVETNAIVSAVEHLAGGDRRWTIDELARFG
HHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHC
GVGGLGPVFVGSPATVADILQEWREHTGVDGFNLAYALAHETFEDVVEYLVPELQRRGAY
CCCCCCCEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PTAYRPGTLREKLFGEGPRLGGTHPGAAYRDLSRFTRGAPTPAAEATPA
CCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SHPIRFNAFEMNCVGHQSPGLWSHPRDRSWQYKDLEYWTDLAKTLEKGIFDGIFIADVI
CCCCEECEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHH
GYYDVYHGSSHHALHQGVQIPVNDPLQLAAPIALATEHLGIGITASTSFEHPYTFARRLS
HHHHHHCCCCCHHHHCCCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHH
TADHHTKGRVGWNIVTSYLESGARNVGQAGLADHTNRYEIAAEYLEVLYKLFEGSWEDGA
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
VLRDRERRIFADPAKIHEIRHHGRHFEVPGYHLCEPSPQRTPVLYQAGASGAGKAFAAQH
HHHCCCCCCCCCHHHHHHHHHCCCEECCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHCC
AECVFVAAPTRATLRDYVADIRRRAAEAGRDAHKLLIYTLVTIVVDETDQKAQAKFDDYL
CCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
RHVSYDGSLVLMSGWTGIDFGQYAPTDLVRHVETNAIVSAVEHLAGGDRRWTIDELARFG
HHHCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHC
GVGGLGPVFVGSPATVADILQEWREHTGVDGFNLAYALAHETFEDVVEYLVPELQRRGAY
CCCCCCCEEECCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
PTAYRPGTLREKLFGEGPRLGGTHPGAAYRDLSRFTRGAPTPAAEATPA
CCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: an alkanesulfonate; O2; FMNH2 [C]

Specific reaction: an alkanesulfonate + O2 + FMNH2 = an aldehyde + SO3 + H2O + FMN [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8892809; 9023192; 1735711 [H]