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The map label for this gene is bigR [H]

Identifier: 238024369

GI number: 238024369

Start: 1180038

End: 1180373

Strand: Reverse

Name: bigR [H]

Synonym: bglu_2g09620

Alternate gene names: 238024369

Gene position: 1180373-1180038 (Counterclockwise)

Preceding gene: 238024371

Following gene: 238024368

Centisome position: 41.75

GC content: 62.8

Gene sequence:

>336_bases
ATGCAAACCCCGTTGTCTCACGCCGCACTCGACTCGCTGCGGCAATCCGCGACGAAGTGCTGTGCCCTTCTCAAGGTCAT
GGCGCACGAGGACCGCTTGCTGCTGTTGTGCCAATTGAGCGAAGGCGAATACAACGTCGGCGAACTGGAGGCGGCGGTCG
GCCTTCGCCAGCCGAACCTTTCGCAACAGCTCGGCGTGCTGCGCGACGAGGGTCTGGTCGTGACGCGGCGCGAGGGAAAG
TACATGTACTACCGCCTCGCGAGCGCCGAAGTCGTGAGCATCATGGAGACCTTGTCGAAACTGTATTGCGGTGACGCCAA
GGGGCGGCCGGCATGA

Upstream 100 bases:

>100_bases
CTGAAGGTCGGGAAAACCTTATCCTGATCAATATATTGAAAAACAATATGTTTTAAATTATATTGACTCGACCTGATCCT
CTGCCGGAAGGAGCCGATCG

Downstream 100 bases:

>100_bases
GCCTCGACCTCGCCCACTTCACGCCCTTGCCCGCGCTCGCCGGCGGCCTGCTGATCGGCCTCGCGGCGACGCTGCTGGTG
CTCGGCAACGGCCGTATCGC

Product: ArsR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 111; Mature: 111

Protein sequence:

>111_residues
MQTPLSHAALDSLRQSATKCCALLKVMAHEDRLLLLCQLSEGEYNVGELEAAVGLRQPNLSQQLGVLRDEGLVVTRREGK
YMYYRLASAEVVSIMETLSKLYCGDAKGRPA

Sequences:

>Translated_111_residues
MQTPLSHAALDSLRQSATKCCALLKVMAHEDRLLLLCQLSEGEYNVGELEAAVGLRQPNLSQQLGVLRDEGLVVTRREGK
YMYYRLASAEVVSIMETLSKLYCGDAKGRPA
>Mature_111_residues
MQTPLSHAALDSLRQSATKCCALLKVMAHEDRLLLLCQLSEGEYNVGELEAAVGLRQPNLSQQLGVLRDEGLVVTRREGK
YMYYRLASAEVVSIMETLSKLYCGDAKGRPA

Specific function: Represses an operon that comprises at least itself and blh. Binds to a palindromic AT-rich sequence spanning the -10 region of the blh promoter and blocks transcription of the operon [H]

COG id: COG0640

COG function: function code K; Predicted transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH arsR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789021, Length=95, Percent_Identity=40, Blast_Score=68, Evalue=1e-13,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001845
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01022 HTH_5 [H]

EC number: NA

Molecular weight: Translated: 12242; Mature: 12242

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS50987 HTH_ARSR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.6 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
3.6 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTPLSHAALDSLRQSATKCCALLKVMAHEDRLLLLCQLSEGEYNVGELEAAVGLRQPNL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCH
SQQLGVLRDEGLVVTRREGKYMYYRLASAEVVSIMETLSKLYCGDAKGRPA
HHHHCCCCCCCEEEEECCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MQTPLSHAALDSLRQSATKCCALLKVMAHEDRLLLLCQLSEGEYNVGELEAAVGLRQPNL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHCCCCCCH
SQQLGVLRDEGLVVTRREGKYMYYRLASAEVVSIMETLSKLYCGDAKGRPA
HHHHCCCCCCCEEEEECCCCEEEEEECHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]