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The map label for this gene is hpaIM [H]

Identifier: 238024260

GI number: 238024260

Start: 1022132

End: 1022980

Strand: Direct

Name: hpaIM [H]

Synonym: bglu_2g08430

Alternate gene names: 238024260

Gene position: 1022132-1022980 (Clockwise)

Preceding gene: 238024259

Following gene: 238024261

Centisome position: 36.15

GC content: 66.9

Gene sequence:

>849_bases
ATGCGCGACCTGATCGAAGAGTCCGCCGGCGCTGCGGCGCGCGAGACCGGCGCGGCTCCGGACGCGGCGCGCGCGCGCGC
GCTGCCCGGCGACATCGACCTGCGCCACCGCGATTTCCTGACCGATGCGGCGAGCCTGCCGGACGCCTCGATCGACCTGA
TCGTCGCCGATCCTCCTTATGGGCTCGGCAAGGACTACGGCAACGACTCGGACAAGCTGCAGGGCGACGCCCATCTGGCA
TGGACCCGCCAGTGGCTCGAGCTGGCGATTCCGAAGCTCAAGGCGAGCGGTTCGCTGTACGTGTTCTGCACCTGGCAATA
CGCGCCCGAGATCTTCAGCTTCCTGAAGACGCGCCTCACGATGGTCAACGAGATCATCTGGGACCGGCGCGTGCCGAGCA
TGGGCGGCACCACGCGGCGCTACACGTCGGTCCACGACAACATCGGGTTTTTCGCGGTATCGAAGAGCTATTACTTCGAC
CTCGATCCGGTCCGCATTCCCTACGACGCCGAAACGAAGAAGGCCCGTTCGCGCAAATTGTTCGAAGGCAGCAAGTGGCT
CGAACTGGGCTACAACCCGAAGGACGTCTGGTCGATTTCGCGGCTGCACCGCCAGCACGCGGAGCGCGTCGACCATCCGA
CCCAGAAGCCGCTCGAACTCGTCGAGCGGATGGTACTGTCGAGCTGCCCGCCGGGCGGCGTGGTGCTGGACCCGTTCATG
GGCAGCGGCACCACCGCCGTGGCGTGTGCCCGGCACGGGCGCGGCTTCGTCGGCTACGAGATCAACGAAAGTTATTGCGC
GATCGCGCGCGAACGCGTGGCCGCGCTCGCCGCGCCGGCGGATGCGTGA

Upstream 100 bases:

>100_bases
CTGCCGAAGGACAAGATCCTGCTCGTCAACCTGTCGGGCCGCGGCGACAAGGACATGCATACCGTCGCCGAGCGCGGCGG
CATCACGCTCTGACGCCCGG

Downstream 100 bases:

>100_bases
GGCGGGCCGGATGCCCGCCTGAGCGCCGCCCATCGTCATCGAATCAAGGAAAGCCACACCATGTCCCGAATCCAGAATAC
CTTCGCAGCACTGGCCGCCC

Product: DNA modification methylase

Products: NA

Alternate protein names: M.HpaI; Adenine-specific methyltransferase HpaI [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MRDLIEESAGAAARETGAAPDAARARALPGDIDLRHRDFLTDAASLPDASIDLIVADPPYGLGKDYGNDSDKLQGDAHLA
WTRQWLELAIPKLKASGSLYVFCTWQYAPEIFSFLKTRLTMVNEIIWDRRVPSMGGTTRRYTSVHDNIGFFAVSKSYYFD
LDPVRIPYDAETKKARSRKLFEGSKWLELGYNPKDVWSISRLHRQHAERVDHPTQKPLELVERMVLSSCPPGGVVLDPFM
GSGTTAVACARHGRGFVGYEINESYCAIARERVAALAAPADA

Sequences:

>Translated_282_residues
MRDLIEESAGAAARETGAAPDAARARALPGDIDLRHRDFLTDAASLPDASIDLIVADPPYGLGKDYGNDSDKLQGDAHLA
WTRQWLELAIPKLKASGSLYVFCTWQYAPEIFSFLKTRLTMVNEIIWDRRVPSMGGTTRRYTSVHDNIGFFAVSKSYYFD
LDPVRIPYDAETKKARSRKLFEGSKWLELGYNPKDVWSISRLHRQHAERVDHPTQKPLELVERMVLSSCPPGGVVLDPFM
GSGTTAVACARHGRGFVGYEINESYCAIARERVAALAAPADA
>Mature_282_residues
MRDLIEESAGAAARETGAAPDAARARALPGDIDLRHRDFLTDAASLPDASIDLIVADPPYGLGKDYGNDSDKLQGDAHLA
WTRQWLELAIPKLKASGSLYVFCTWQYAPEIFSFLKTRLTMVNEIIWDRRVPSMGGTTRRYTSVHDNIGFFAVSKSYYFD
LDPVRIPYDAETKKARSRKLFEGSKWLELGYNPKDVWSISRLHRQHAERVDHPTQKPLELVERMVLSSCPPGGVVLDPFM
GSGTTAVACARHGRGFVGYEINESYCAIARERVAALAAPADA

Specific function: This methylase recognizes the double-stranded sequence GTTAAC, causes specific methylation on A-5 on both strands, and protects the DNA from cleavage by the HpaI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=243, Percent_Identity=31.2757201646091, Blast_Score=121, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31305; Mature: 31305

Theoretical pI: Translated: 6.88; Mature: 6.88

Prosite motif: PS00092 N6_MTASE ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDLIEESAGAAARETGAAPDAARARALPGDIDLRHRDFLTDAASLPDASIDLIVADPPY
CHHHHHHHCCCHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCC
GLGKDYGNDSDKLQGDAHLAWTRQWLELAIPKLKASGSLYVFCTWQYAPEIFSFLKTRLT
CCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHEECCCCEEEEEEECHHHHHHHHHHHHHH
MVNEIIWDRRVPSMGGTTRRYTSVHDNIGFFAVSKSYYFDLDPVRIPYDAETKKARSRKL
HHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEECCEEEECCCEECCCCCHHHHHHHHHH
FEGSKWLELGYNPKDVWSISRLHRQHAERVDHPTQKPLELVERMVLSSCPPGGVVLDPFM
HCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEECCCC
GSGTTAVACARHGRGFVGYEINESYCAIARERVAALAAPADA
CCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MRDLIEESAGAAARETGAAPDAARARALPGDIDLRHRDFLTDAASLPDASIDLIVADPPY
CHHHHHHHCCCHHHHCCCCCCHHHHHCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCC
GLGKDYGNDSDKLQGDAHLAWTRQWLELAIPKLKASGSLYVFCTWQYAPEIFSFLKTRLT
CCCCCCCCCCHHCCCCHHHHHHHHHHHHHHHHEECCCCEEEEEEECHHHHHHHHHHHHHH
MVNEIIWDRRVPSMGGTTRRYTSVHDNIGFFAVSKSYYFDLDPVRIPYDAETKKARSRKL
HHHHHHHHCCCCCCCCCCCEEEECCCCCCEEEEECCEEEECCCEECCCCCHHHHHHHHHH
FEGSKWLELGYNPKDVWSISRLHRQHAERVDHPTQKPLELVERMVLSSCPPGGVVLDPFM
HCCCCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCEEECCCC
GSGTTAVACARHGRGFVGYEINESYCAIARERVAALAAPADA
CCCCHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1542567 [H]