Click here to switch to the map view.

The map label for this gene is mdh2 [H]

Identifier: 238024239

GI number: 238024239

Start: 1000118

End: 1001101

Strand: Reverse

Name: mdh2 [H]

Synonym: bglu_2g08210

Alternate gene names: 238024239

Gene position: 1001101-1000118 (Counterclockwise)

Preceding gene: 238024249

Following gene: 238024238

Centisome position: 35.41

GC content: 66.57

Gene sequence:

>984_bases
ATGGCTAAGCCCGCAAAGCGTGTTGCCGTTACCGGCGCCGCAGGTCAAATCGCATACTCGCTGCTGTTCCGCATCGCGAA
CGGCGACCTGCTCGGCAAGGACCAGCCGGTCATCCTGCAACTGCTCGACCTCCCGCAAGCACAAGGCGCCGTCAAGGGCG
TCGTGATGGAACTCGACGACTGCGCGTTCCCGCTGCTGGCCGGCGTGGTGATCACGGACGACCCGAAGGTCGCGTTCAAG
GATGCCGACGTGGCGCTGCTGGTCGGGGCGCGTCCGCGCTCGAAGGGCATGGAGCGCAAGGACCTGCTGTCGGCCAACGC
CGAGATCTTCACGGTGCAGGGTGCCGCGCTGAACGAAGTCGCGAGCCGCGACGTGAAGGTGCTGGTGGTCGGCAACCCGG
CCAACACCAACGCCTACATCGCGATGAAGTCGGCGCCGGACCTGCCGAAGAAGAACTTCACGGCGATGCTGCGCCTGGAC
CACAACCGCGCGCTGTCGCAACTGGCCGCCAAGGCCGGCAAGCCCGTGGCCTCGATCGAGAAGCTCGCCGTGTGGGGCAA
CCACTCGCCGACGATGTACCCCGACTTCCGCTTCGCGACCGCCGAAGGCGAATCGCTGCTCAAGCTGATCAACGACGACG
CCTGGAACCGCGACACCTTCATCCCGACCGTCGGCAAGCGCGGCGCGGCGATCATCGAGGCGCGCGGCCTGTCGTCGGCG
GCCTCGGCGGCCAACGCGGCGATCGATCACGTGCGTGACTGGGTGCTCGGCACGAACGGCAAGTGGGTCACCATGGGCAT
CCCGTCGGACGGCTCGTACGGCATCCCCGAGGACATCATCTACGGCGTGCCGGTGACTTGCGAAAACGGCGAGTACAAGC
GCGTGGAAGGCCTGGAAATCGACGCGTTCTCGCGCGAGAAGATGGACGGCACGCTGGCCGAGCTGCTCGAGGAACGTGAC
GGCGTGGCCCACCTGCTGAAGTAA

Upstream 100 bases:

>100_bases
GCGAGTCCTGCGCCCCGCTTCAACGCATCGCTACGCGTAACGCGCATAGAATGGCGTTTTCCCTAGCGTCCTACGCTCAT
CGTCCTGGAGATTTCTCAAC

Downstream 100 bases:

>100_bases
GTTCCGCGCTTCAGTGCCGCCGCCGGCCCGCGCGGCGGCCCGGCCGCCCGGCTCCCGCCGGCGGCCGGCCAGGCCCCGCA
TTCGATCCGCACGCGCGTTG

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MAKPAKRVAVTGAAGQIAYSLLFRIANGDLLGKDQPVILQLLDLPQAQGAVKGVVMELDDCAFPLLAGVVITDDPKVAFK
DADVALLVGARPRSKGMERKDLLSANAEIFTVQGAALNEVASRDVKVLVVGNPANTNAYIAMKSAPDLPKKNFTAMLRLD
HNRALSQLAAKAGKPVASIEKLAVWGNHSPTMYPDFRFATAEGESLLKLINDDAWNRDTFIPTVGKRGAAIIEARGLSSA
ASAANAAIDHVRDWVLGTNGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEIDAFSREKMDGTLAELLEERD
GVAHLLK

Sequences:

>Translated_327_residues
MAKPAKRVAVTGAAGQIAYSLLFRIANGDLLGKDQPVILQLLDLPQAQGAVKGVVMELDDCAFPLLAGVVITDDPKVAFK
DADVALLVGARPRSKGMERKDLLSANAEIFTVQGAALNEVASRDVKVLVVGNPANTNAYIAMKSAPDLPKKNFTAMLRLD
HNRALSQLAAKAGKPVASIEKLAVWGNHSPTMYPDFRFATAEGESLLKLINDDAWNRDTFIPTVGKRGAAIIEARGLSSA
ASAANAAIDHVRDWVLGTNGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEIDAFSREKMDGTLAELLEERD
GVAHLLK
>Mature_326_residues
AKPAKRVAVTGAAGQIAYSLLFRIANGDLLGKDQPVILQLLDLPQAQGAVKGVVMELDDCAFPLLAGVVITDDPKVAFKD
ADVALLVGARPRSKGMERKDLLSANAEIFTVQGAALNEVASRDVKVLVVGNPANTNAYIAMKSAPDLPKKNFTAMLRLDH
NRALSQLAAKAGKPVASIEKLAVWGNHSPTMYPDFRFATAEGESLLKLINDDAWNRDTFIPTVGKRGAAIIEARGLSSAA
SAANAAIDHVRDWVLGTNGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEIDAFSREKMDGTLAELLEERDG
VAHLLK

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate [H]

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 2 family [H]

Homologues:

Organism=Homo sapiens, GI5174539, Length=332, Percent_Identity=54.5180722891566, Blast_Score=354, Evalue=6e-98,
Organism=Homo sapiens, GI89886456, Length=333, Percent_Identity=24.6246246246246, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17561064, Length=320, Percent_Identity=54.6875, Blast_Score=325, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI32566798, Length=257, Percent_Identity=55.2529182879377, Blast_Score=259, Evalue=2e-69,
Organism=Caenorhabditis elegans, GI32566800, Length=170, Percent_Identity=50, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24583394, Length=325, Percent_Identity=56, Blast_Score=341, Evalue=3e-94,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001557
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR010945
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N [H]

EC number: =1.1.1.37 [H]

Molecular weight: Translated: 34945; Mature: 34814

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKPAKRVAVTGAAGQIAYSLLFRIANGDLLGKDQPVILQLLDLPQAQGAVKGVVMELDD
CCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCHHHEEEEECCC
CAFPLLAGVVITDDPKVAFKDADVALLVGARPRSKGMERKDLLSANAEIFTVQGAALNEV
HHHHHHCCEEEECCCCEEEECCCEEEEEECCCCCCCCCHHHHHCCCCEEEEECCCHHHHH
ASRDVKVLVVGNPANTNAYIAMKSAPDLPKKNFTAMLRLDHNRALSQLAAKAGKPVASIE
HCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHH
KLAVWGNHSPTMYPDFRFATAEGESLLKLINDDAWNRDTFIPTVGKRGAAIIEARGLSSA
HEEEECCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHH
ASAANAAIDHVRDWVLGTNGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEI
HHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCCCHHHEECCEEEECCCCEEEECCEEE
DAFSREKMDGTLAELLEERDGVAHLLK
CCCCCHHCCCHHHHHHHHCCCHHHHCC
>Mature Secondary Structure 
AKPAKRVAVTGAAGQIAYSLLFRIANGDLLGKDQPVILQLLDLPQAQGAVKGVVMELDD
CCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCCCCHHHHEECCCCCCCHHHEEEEECCC
CAFPLLAGVVITDDPKVAFKDADVALLVGARPRSKGMERKDLLSANAEIFTVQGAALNEV
HHHHHHCCEEEECCCCEEEECCCEEEEEECCCCCCCCCHHHHHCCCCEEEEECCCHHHHH
ASRDVKVLVVGNPANTNAYIAMKSAPDLPKKNFTAMLRLDHNRALSQLAAKAGKPVASIE
HCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCEEEEEECCHHHHHHHHHHCCCCHHHHH
KLAVWGNHSPTMYPDFRFATAEGESLLKLINDDAWNRDTFIPTVGKRGAAIIEARGLSSA
HEEEECCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHH
ASAANAAIDHVRDWVLGTNGKWVTMGIPSDGSYGIPEDIIYGVPVTCENGEYKRVEGLEI
HHHHHHHHHHHHHHEECCCCCEEEEECCCCCCCCCCHHHEECCEEEECCCCEEEECCEEE
DAFSREKMDGTLAELLEERDGVAHLLK
CCCCCHHCCCHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA