Click here to switch to the map view.

The map label for this gene is bcr [H]

Identifier: 238024175

GI number: 238024175

Start: 910902

End: 912161

Strand: Reverse

Name: bcr [H]

Synonym: bglu_2g07540

Alternate gene names: 238024175

Gene position: 912161-910902 (Counterclockwise)

Preceding gene: 238024176

Following gene: 238024174

Centisome position: 32.26

GC content: 68.81

Gene sequence:

>1260_bases
ATGAACCCCGATACGCCAAGCCGTTCCGTGCAGGCGAGCGCCGCCAATTCGCCGCGCTTCCTGCTGCTCCTGATCTGTCT
GTTCGCGTCCGCCGGACAACTCGCGATCGACATCTACGTGCCGGCGCTGCCGGCCATGGCGCGTGACTTCTCGACCTCGC
CGCAGGCGATCCAGTCCAGCGTGTCCGGCTACATGGCGGCCTACGCGCTGGGCCAGCTGATCTTCGGACCGGTGGCCGAC
GCCTACGGCCGCAAGCGCGTGCTCGGCTTCGGGCTCGTGATCTACACGGCCGGCTGCCTGCTGTCGCTGGCCGCGCCGAA
CCTCGAGACCTTCCTGCTCGCGCGCTGCCTGCAGGGCTTCGGCATCGCCACCACGAACCTGCTGGCCAAGGCGATCATCA
CCGATTCGTTCTCGGGCCAGGCGCTGGTCCACGCCTTCACCTACATGGCGATCGCCTGGGGCGTCGCGCCGATCATCGCG
CCGGTGATCGGCGCGCATCTGCAGACCTGGTTCGGCTGGCGGGCCTGCCTCGTGTTCCTGCTGATCTATTCGCTGACGAT
GTGGGCGATCCTGTGGCGCTTTCGCGAGACGCTCAGGCAGCCGGTGCGGCTCGCGCCGCGCACGCTCGCGGGCAACGCGG
GCAAGGTGCTGGCGAGCCCGGTGTTCCAGAGCTGCTTCCTCGCGCAGGGCGTGTGCTACAGCATCCTGCTGGTGTTCAAC
ATCGTCGGCCCGTTCATGGTGCAGACCACGCTGCACGAGCCGCCCACCTTCTTCGGCTATCTCGCGCTCGCGATCGGTGC
GATGTACTTCTTCGGCGGGCTGTCGAACCGGATTCACAGCGCGGCGCTGCCGAGTTCCGAGGCCCGGCTGCGGATCGGCG
CCTACCTGATGGCCGGATCGGCGCTCGCGATGCTGGCGCTCGCGCTGACGGTCGGCCTGCGTGTCTGGACGCTCGCGCTG
CCGGTGATGGTGATGGGCTTCTGCGCGGGCGCGATGTACCCGACGCTGATGGCCAAGGGCAATTCGCTGTTCCCGCATAT
CGCCGGCCTGACGAGCGCGATCCTGGGCTGCGCGCTGCTGCTCGTCTCGGCGGCGATGATGGGGCTCGCCGGCTTCGTAT
CGGTCCACGAGCTGACGCCGCTCGCGGTGTTCTTCGTGATCCTGGCCGGCATCGTGGTGGTGACCGTCACCCGGCTGCTG
CGCCATCTGTCCCACGCCGCGCCGCCGCCGGTGGCCTGCAAGGCCGGCAACGCCGCCTGA

Upstream 100 bases:

>100_bases
CCACCGACGCTCCGCGCCCCGCCCGCTGTTCACCATGCGAACTCGGCAGGAGCCTCGCACGCCCCGCTTCGCGCGCCGCC
GCCCCGTTTCCGATGCCTTC

Downstream 100 bases:

>100_bases
GCGGGGCCGCCGCGTACCCTCCTCGCCGGTCGCGCTGGCGCGCTGGCCGGCCGCTTTCCTCGCCTCCATGCGCGGTCCCG
CCGTGCCATCCCCGCCGCGC

Product: major facilitator superfamily protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Sulfonamide resistance protein [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNPDTPSRSVQASAANSPRFLLLLICLFASAGQLAIDIYVPALPAMARDFSTSPQAIQSSVSGYMAAYALGQLIFGPVAD
AYGRKRVLGFGLVIYTAGCLLSLAAPNLETFLLARCLQGFGIATTNLLAKAIITDSFSGQALVHAFTYMAIAWGVAPIIA
PVIGAHLQTWFGWRACLVFLLIYSLTMWAILWRFRETLRQPVRLAPRTLAGNAGKVLASPVFQSCFLAQGVCYSILLVFN
IVGPFMVQTTLHEPPTFFGYLALAIGAMYFFGGLSNRIHSAALPSSEARLRIGAYLMAGSALAMLALALTVGLRVWTLAL
PVMVMGFCAGAMYPTLMAKGNSLFPHIAGLTSAILGCALLLVSAAMMGLAGFVSVHELTPLAVFFVILAGIVVVTVTRLL
RHLSHAAPPPVACKAGNAA

Sequences:

>Translated_419_residues
MNPDTPSRSVQASAANSPRFLLLLICLFASAGQLAIDIYVPALPAMARDFSTSPQAIQSSVSGYMAAYALGQLIFGPVAD
AYGRKRVLGFGLVIYTAGCLLSLAAPNLETFLLARCLQGFGIATTNLLAKAIITDSFSGQALVHAFTYMAIAWGVAPIIA
PVIGAHLQTWFGWRACLVFLLIYSLTMWAILWRFRETLRQPVRLAPRTLAGNAGKVLASPVFQSCFLAQGVCYSILLVFN
IVGPFMVQTTLHEPPTFFGYLALAIGAMYFFGGLSNRIHSAALPSSEARLRIGAYLMAGSALAMLALALTVGLRVWTLAL
PVMVMGFCAGAMYPTLMAKGNSLFPHIAGLTSAILGCALLLVSAAMMGLAGFVSVHELTPLAVFFVILAGIVVVTVTRLL
RHLSHAAPPPVACKAGNAA
>Mature_419_residues
MNPDTPSRSVQASAANSPRFLLLLICLFASAGQLAIDIYVPALPAMARDFSTSPQAIQSSVSGYMAAYALGQLIFGPVAD
AYGRKRVLGFGLVIYTAGCLLSLAAPNLETFLLARCLQGFGIATTNLLAKAIITDSFSGQALVHAFTYMAIAWGVAPIIA
PVIGAHLQTWFGWRACLVFLLIYSLTMWAILWRFRETLRQPVRLAPRTLAGNAGKVLASPVFQSCFLAQGVCYSILLVFN
IVGPFMVQTTLHEPPTFFGYLALAIGAMYFFGGLSNRIHSAALPSSEARLRIGAYLMAGSALAMLALALTVGLRVWTLAL
PVMVMGFCAGAMYPTLMAKGNSLFPHIAGLTSAILGCALLLVSAAMMGLAGFVSVHELTPLAVFFVILAGIVVVTVTRLL
RHLSHAAPPPVACKAGNAA

Specific function: Involved in sulfonamide (sulfathiazole) and bicyclomycin resistance. Probable membrane translocase [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Bcr/CmlA family [H]

Homologues:

Organism=Escherichia coli, GI1788509, Length=369, Percent_Identity=27.3712737127371, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI48994889, Length=346, Percent_Identity=29.1907514450867, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI87082312, Length=341, Percent_Identity=28.1524926686217, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1787065, Length=153, Percent_Identity=28.1045751633987, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790794, Length=158, Percent_Identity=28.4810126582279, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790195, Length=135, Percent_Identity=31.8518518518519, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1790146, Length=239, Percent_Identity=26.3598326359833, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004812
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 44346; Mature: 44346

Theoretical pI: Translated: 9.67; Mature: 9.67

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPDTPSRSVQASAANSPRFLLLLICLFASAGQLAIDIYVPALPAMARDFSTSPQAIQSS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCHHHHHHH
VSGYMAAYALGQLIFGPVADAYGRKRVLGFGLVIYTAGCLLSLAAPNLETFLLARCLQGF
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
GIATTNLLAKAIITDSFSGQALVHAFTYMAIAWGVAPIIAPVIGAHLQTWFGWRACLVFL
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIYSLTMWAILWRFRETLRQPVRLAPRTLAGNAGKVLASPVFQSCFLAQGVCYSILLVFN
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IVGPFMVQTTLHEPPTFFGYLALAIGAMYFFGGLSNRIHSAALPSSEARLRIGAYLMAGS
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ALAMLALALTVGLRVWTLALPVMVMGFCAGAMYPTLMAKGNSLFPHIAGLTSAILGCALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LVSAAMMGLAGFVSVHELTPLAVFFVILAGIVVVTVTRLLRHLSHAAPPPVACKAGNAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MNPDTPSRSVQASAANSPRFLLLLICLFASAGQLAIDIYVPALPAMARDFSTSPQAIQSS
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCHHHHHHH
VSGYMAAYALGQLIFGPVADAYGRKRVLGFGLVIYTAGCLLSLAAPNLETFLLARCLQGF
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCC
GIATTNLLAKAIITDSFSGQALVHAFTYMAIAWGVAPIIAPVIGAHLQTWFGWRACLVFL
CCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIYSLTMWAILWRFRETLRQPVRLAPRTLAGNAGKVLASPVFQSCFLAQGVCYSILLVFN
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
IVGPFMVQTTLHEPPTFFGYLALAIGAMYFFGGLSNRIHSAALPSSEARLRIGAYLMAGS
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
ALAMLALALTVGLRVWTLALPVMVMGFCAGAMYPTLMAKGNSLFPHIAGLTSAILGCALL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
LVSAAMMGLAGFVSVHELTPLAVFFVILAGIVVVTVTRLLRHLSHAAPPPVACKAGNAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8486276; 9278503; 2694948 [H]