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The map label for this gene is lig [H]

Identifier: 238024152

GI number: 238024152

Start: 867436

End: 869103

Strand: Direct

Name: lig [H]

Synonym: bglu_2g07300

Alternate gene names: 238024152

Gene position: 867436-869103 (Clockwise)

Preceding gene: 238024151

Following gene: 238024153

Centisome position: 30.68

GC content: 72.84

Gene sequence:

>1668_bases
ATGAAGCGCTTCGCGACGCTCTACGCCGCGCTCGACGCGACCACCTCCACCAACGTCAAGCTCGAGGCGCTGATCGCCTA
TTTCGCGAGCGCCGCGCCCGAGGATGCGGCCTGGGCCTCGTATTTCCTCGCGGGCGGCAAGCCGCGCCAGACCGTGCCCA
CGCGCGTGCTGGCCGAGGCCGCGCGCGAGCGCGCCGGCCTGCCGGCCTGGTTGTTCGAGGAGTCGTACCAGGCGGTGGGC
GACCTGGCCGAGACGATCGCCCACGTGCTGCCGCCGCCCGCGCGCGAATCGGCGCTGGGGCTCGCGCAGTGGATCGAGCA
GCGCGTGCTGGGCCTGCGCGGCCTCGATGCCGCGGCGCTGCGCGAGCGCCTGGCCGGCTACTGGGACGAACTCGACTGGA
GCGAGCGCTTCCTGCTCACCAAGCTGATCGGCGGCGGCTTCCGGGTGGGCGTGTCGCGCCAGCTGGTGGTGCGCGCGCTC
GCGCAGGTGGCGGGCGTCGATCACAAGCGCATCGCGCAGCGCATGGTCGGCTGGACCGATTCGCGCCAGACGCCGAGCGC
CGCGCGTTATCTGCGGCTGATCGCGCCGGCCGGCGACGGCGACGATCCGGCGAGGCCGCACGAAAGCGACCTCGGCCTGC
CGTATCCGTTCTTCCTCGCCCACCCGCTGCAGGCCGATCCGGCCACGCTCGGGCCGGTCGAGGACTGGATCGTCGAATGG
AAGTGGGACGGCATTCGCGCCCAGCTCGTCAAGCGCGCGGGCCGCGTCTGGCTCTGGTCGCGCGGCGAGGACCTCGTGAC
CGAGCGTTTTCCGGAGCTGGCCGCGCTCGGCGAGGCACTGCCCGACGGCACCGTGATCGACGGCGAGATTCTCGCCTGGG
AGCCGGGCGCCGCTGCGCCGCTGCCGTTCGCGCGCCTGCAGCCGCGCATCACGCGCAAGTCCCTGACCAGGAAGGTGCTG
GCCGATTCCCCGGCGGCGCTGCTCGCCTACGACCTGCTCGAAGCCGAGGGCCGCGACCTGCGCGCCGTGCCGCTCGCGAC
GCGGCGCACGCGGCTCGAGGCGCTGGCCGCGGCGCTCTCGCGCACGCTCGCCGTCGATCTGCTGCGGGTCTCGCCGCGCG
TGACGGCGGACGGCTGGCCGGCGCTCGCCGCGCGGCGCGCCGAGAGCCGCGCGCGCGGCGTGGAAGGGCTGATGCTGAAG
GCGGCGGCGTCGGAGTACGGCGTCGGCCGCACCAAGGCCTCCGGGGTCTGGTGGAAGTGGAAGATCGAGCCGTATGCGAT
CGACGCGGTGCTGCTCTACGCGCAGCGCGGCCACGGCCGGCGCGCGAGCCTCTATACCGACTTCACGTTCGCGGTGTGGG
ACGAGGCCGGCGGCGTGCGTACCCTCGTGCCGTTCGCCAAGGCCTATTCCGGGCTGACCGACGAGGAGATGCGCCAGGTG
GACGCGGTGGTGCGCAAGACCACCATCGAGAAGTTCGGCCCGGTGCGCAGCGTCACGCCCACGCTGGTGTTCGAGATCGG
CTTCGAAGGCATCCAGGCGAGCCCGCGCCACAAGTCGGGCATCGCCGTGCGGTTCCCGCGCATGCTGCGCTGGCGCACCG
ACAAGTCGATCGACGAGGCGGACACGCTGGAGATGCTCAAGGGCTTTCTGAACGGAGCGCCGGCATGA

Upstream 100 bases:

>100_bases
AGGGGCTCGACGCCGGCGCGTTCGCCACCGAATACGGCGACGAGGCGCTCGATGCGGGGGACCCCGCCGAGGCGGCCGGG
CCTAGCGCGGGCGGGCCGCG

Downstream 100 bases:

>100_bases
GCGGGGCGCGCGGCACCGGCGAGCGGCACGGCCGTGAACACGATGGCGCCGCTGCCGGCGGCGATCCCGCCGCGTCGCGC
GACGCCGCGGATGCGGCGGA

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MKRFATLYAALDATTSTNVKLEALIAYFASAAPEDAAWASYFLAGGKPRQTVPTRVLAEAARERAGLPAWLFEESYQAVG
DLAETIAHVLPPPARESALGLAQWIEQRVLGLRGLDAAALRERLAGYWDELDWSERFLLTKLIGGGFRVGVSRQLVVRAL
AQVAGVDHKRIAQRMVGWTDSRQTPSAARYLRLIAPAGDGDDPARPHESDLGLPYPFFLAHPLQADPATLGPVEDWIVEW
KWDGIRAQLVKRAGRVWLWSRGEDLVTERFPELAALGEALPDGTVIDGEILAWEPGAAAPLPFARLQPRITRKSLTRKVL
ADSPAALLAYDLLEAEGRDLRAVPLATRRTRLEALAAALSRTLAVDLLRVSPRVTADGWPALAARRAESRARGVEGLMLK
AAASEYGVGRTKASGVWWKWKIEPYAIDAVLLYAQRGHGRRASLYTDFTFAVWDEAGGVRTLVPFAKAYSGLTDEEMRQV
DAVVRKTTIEKFGPVRSVTPTLVFEIGFEGIQASPRHKSGIAVRFPRMLRWRTDKSIDEADTLEMLKGFLNGAPA

Sequences:

>Translated_555_residues
MKRFATLYAALDATTSTNVKLEALIAYFASAAPEDAAWASYFLAGGKPRQTVPTRVLAEAARERAGLPAWLFEESYQAVG
DLAETIAHVLPPPARESALGLAQWIEQRVLGLRGLDAAALRERLAGYWDELDWSERFLLTKLIGGGFRVGVSRQLVVRAL
AQVAGVDHKRIAQRMVGWTDSRQTPSAARYLRLIAPAGDGDDPARPHESDLGLPYPFFLAHPLQADPATLGPVEDWIVEW
KWDGIRAQLVKRAGRVWLWSRGEDLVTERFPELAALGEALPDGTVIDGEILAWEPGAAAPLPFARLQPRITRKSLTRKVL
ADSPAALLAYDLLEAEGRDLRAVPLATRRTRLEALAAALSRTLAVDLLRVSPRVTADGWPALAARRAESRARGVEGLMLK
AAASEYGVGRTKASGVWWKWKIEPYAIDAVLLYAQRGHGRRASLYTDFTFAVWDEAGGVRTLVPFAKAYSGLTDEEMRQV
DAVVRKTTIEKFGPVRSVTPTLVFEIGFEGIQASPRHKSGIAVRFPRMLRWRTDKSIDEADTLEMLKGFLNGAPA
>Mature_555_residues
MKRFATLYAALDATTSTNVKLEALIAYFASAAPEDAAWASYFLAGGKPRQTVPTRVLAEAARERAGLPAWLFEESYQAVG
DLAETIAHVLPPPARESALGLAQWIEQRVLGLRGLDAAALRERLAGYWDELDWSERFLLTKLIGGGFRVGVSRQLVVRAL
AQVAGVDHKRIAQRMVGWTDSRQTPSAARYLRLIAPAGDGDDPARPHESDLGLPYPFFLAHPLQADPATLGPVEDWIVEW
KWDGIRAQLVKRAGRVWLWSRGEDLVTERFPELAALGEALPDGTVIDGEILAWEPGAAAPLPFARLQPRITRKSLTRKVL
ADSPAALLAYDLLEAEGRDLRAVPLATRRTRLEALAAALSRTLAVDLLRVSPRVTADGWPALAARRAESRARGVEGLMLK
AAASEYGVGRTKASGVWWKWKIEPYAIDAVLLYAQRGHGRRASLYTDFTFAVWDEAGGVRTLVPFAKAYSGLTDEEMRQV
DAVVRKTTIEKFGPVRSVTPTLVFEIGFEGIQASPRHKSGIAVRFPRMLRWRTDKSIDEADTLEMLKGFLNGAPA

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=363, Percent_Identity=25.8953168044077, Blast_Score=86, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71985269, Length=468, Percent_Identity=26.7094017094017, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320038, Length=362, Percent_Identity=25.6906077348066, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI85815841, Length=347, Percent_Identity=26.2247838616715, Blast_Score=80, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 61025; Mature: 61025

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: PS00697 DNA_LIGASE_A1 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRFATLYAALDATTSTNVKLEALIAYFASAAPEDAAWASYFLAGGKPRQTVPTRVLAEA
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHH
ARERAGLPAWLFEESYQAVGDLAETIAHVLPPPARESALGLAQWIEQRVLGLRGLDAAAL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
RERLAGYWDELDWSERFLLTKLIGGGFRVGVSRQLVVRALAQVAGVDHKRIAQRMVGWTD
HHHHHHHHHCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
SRQTPSAARYLRLIAPAGDGDDPARPHESDLGLPYPFFLAHPLQADPATLGPVEDWIVEW
CCCCCHHHHHEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHEEEE
KWDGIRAQLVKRAGRVWLWSRGEDLVTERFPELAALGEALPDGTVIDGEILAWEPGAAAP
EECCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEECCEEEEECCCCCCC
LPFARLQPRITRKSLTRKVLADSPAALLAYDLLEAEGRDLRAVPLATRRTRLEALAAALS
CCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHH
RTLAVDLLRVSPRVTADGWPALAARRAESRARGVEGLMLKAAASEYGVGRTKASGVWWKW
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEE
KIEPYAIDAVLLYAQRGHGRRASLYTDFTFAVWDEAGGVRTLVPFAKAYSGLTDEEMRQV
EECCEEEEEEEEEECCCCCCEEEEEECEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHH
DAVVRKTTIEKFGPVRSVTPTLVFEIGFEGIQASPRHKSGIAVRFPRMLRWRTDKSIDEA
HHHHHHHHHHHHCCCCCCCHHEEEEECCCCCCCCCCCCCCCEEECHHHHHHCCCCCCCHH
DTLEMLKGFLNGAPA
HHHHHHHHHHCCCCC
>Mature Secondary Structure
MKRFATLYAALDATTSTNVKLEALIAYFASAAPEDAAWASYFLAGGKPRQTVPTRVLAEA
CCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHH
ARERAGLPAWLFEESYQAVGDLAETIAHVLPPPARESALGLAQWIEQRVLGLRGLDAAAL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHH
RERLAGYWDELDWSERFLLTKLIGGGFRVGVSRQLVVRALAQVAGVDHKRIAQRMVGWTD
HHHHHHHHHCCCHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHHCCCHHHHHHHHHCCCC
SRQTPSAARYLRLIAPAGDGDDPARPHESDLGLPYPFFLAHPLQADPATLGPVEDWIVEW
CCCCCHHHHHEEEEECCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCHHHHEEEE
KWDGIRAQLVKRAGRVWLWSRGEDLVTERFPELAALGEALPDGTVIDGEILAWEPGAAAP
EECCHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCEEECCEEEEECCCCCCC
LPFARLQPRITRKSLTRKVLADSPAALLAYDLLEAEGRDLRAVPLATRRTRLEALAAALS
CCHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHH
RTLAVDLLRVSPRVTADGWPALAARRAESRARGVEGLMLKAAASEYGVGRTKASGVWWKW
HHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCEEEEE
KIEPYAIDAVLLYAQRGHGRRASLYTDFTFAVWDEAGGVRTLVPFAKAYSGLTDEEMRQV
EECCEEEEEEEEEECCCCCCEEEEEECEEEEEECCCCCCCHHHHHHHHHCCCCHHHHHHH
DAVVRKTTIEKFGPVRSVTPTLVFEIGFEGIQASPRHKSGIAVRFPRMLRWRTDKSIDEA
HHHHHHHHHHHHCCCCCCCHHEEEEECCCCCCCCCCCCCCCEEECHHHHHHCCCCCCCHH
DTLEMLKGFLNGAPA
HHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA