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The map label for this gene is mhpE [H]

Identifier: 238024018

GI number: 238024018

Start: 689229

End: 690146

Strand: Direct

Name: mhpE [H]

Synonym: bglu_2g05720

Alternate gene names: 238024018

Gene position: 689229-690146 (Clockwise)

Preceding gene: 238024017

Following gene: 238024019

Centisome position: 24.38

GC content: 67.86

Gene sequence:

>918_bases
ATGACCTTGCTGTCGAATCAGGCGCATTGCGCGCGCGCCGTCACGCTGCTCGACTGCACCCTGCGGGACGGCGGTTACCT
CAATGGCCATGATTTTTCCGCGCCGCAGATCGACGCGATCCTGACGCGGCTGCTCGCCTCGGGCGTGGATGTGATCGAGG
TCGGCTATTTCCGGCGGCGCAACGTCGAACGGCACCCGTCCACGGTTATCGCACGCTGCCCGCCCGCCTTCCTCGCTTCG
CTGGCGCGCACGCAAGCTGTCCGCTATGCGGTGATGGTGCCGCCCGGCGACGCCGAGCCGGCCGATCTCGACGAACTCGC
CGAGTTGCCGGTCTCGGTGGTCCGCTTCACCTGCACCGACCAGAACCTGCCGAAGGTGCTCGCGCTCGGCGAGCGCGCCG
CGCGGCTCGGTCTCGTGGTCTGCGCCAACATCACGCGGGTGAGCGAGCGCTCGCACTCGCAGCTCGCCGCGCTCACGCGC
GCCCTGGCCGAGGCCGGCTTCGACACGGTCTACGCGGCCGATTCGAACGGCGCGCTCTACCCGGACGAGGTCGCCGCGAT
CGTCGGCGAGATGCGCCGTCATTTCACCGGCCAGATCGGCTTTCATGCGCACGACTCGCTGCATCTGGCGTTCGCGAACG
CCCTGGCCGCGATGCGCGCGGGCGCGTCGTGGGTCGACGGCACGATCGGCGGAGTCGGCAAGGGTGGCGGCAACGCGGCG
ATCGAGGTGCTGCTCTCGGAGCTCGCGAAGGCCGATTCACGGCCGATCGACATCGCCGGATTCTCGCAGATGCCGGCGAG
CTTTCCGCACGACTTCTTTCCGGTGCGTTTCAAGGAGCGCCTCGACAACGTCTTTTACGGCCGCAACAACCTGAACATCG
ACAAGATCAAGTCCCTCGAGTCCCGACCCTCCCACTGA

Upstream 100 bases:

>100_bases
GCTCAACTTTCCGTCTGTTTGCGGCCGGCGAGTGAGGTATTGCACTCGGCATGCATGATGATGCGCGTAATACGCGCGTT
CGAATCGTGGAGTTCAGGCT

Downstream 100 bases:

>100_bases
CAGGAACCGCACCATGAGCGAATTCGTTCAATCCCTGCGCGACAAGATCAACAGCCGAAAGAAGATGACCAGCCGGCTCT
ACCAGACGGTGCTGGCCGGC

Product: MhpE-like protein

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase [H]

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MTLLSNQAHCARAVTLLDCTLRDGGYLNGHDFSAPQIDAILTRLLASGVDVIEVGYFRRRNVERHPSTVIARCPPAFLAS
LARTQAVRYAVMVPPGDAEPADLDELAELPVSVVRFTCTDQNLPKVLALGERAARLGLVVCANITRVSERSHSQLAALTR
ALAEAGFDTVYAADSNGALYPDEVAAIVGEMRRHFTGQIGFHAHDSLHLAFANALAAMRAGASWVDGTIGGVGKGGGNAA
IEVLLSELAKADSRPIDIAGFSQMPASFPHDFFPVRFKERLDNVFYGRNNLNIDKIKSLESRPSH

Sequences:

>Translated_305_residues
MTLLSNQAHCARAVTLLDCTLRDGGYLNGHDFSAPQIDAILTRLLASGVDVIEVGYFRRRNVERHPSTVIARCPPAFLAS
LARTQAVRYAVMVPPGDAEPADLDELAELPVSVVRFTCTDQNLPKVLALGERAARLGLVVCANITRVSERSHSQLAALTR
ALAEAGFDTVYAADSNGALYPDEVAAIVGEMRRHFTGQIGFHAHDSLHLAFANALAAMRAGASWVDGTIGGVGKGGGNAA
IEVLLSELAKADSRPIDIAGFSQMPASFPHDFFPVRFKERLDNVFYGRNNLNIDKIKSLESRPSH
>Mature_304_residues
TLLSNQAHCARAVTLLDCTLRDGGYLNGHDFSAPQIDAILTRLLASGVDVIEVGYFRRRNVERHPSTVIARCPPAFLASL
ARTQAVRYAVMVPPGDAEPADLDELAELPVSVVRFTCTDQNLPKVLALGERAARLGLVVCANITRVSERSHSQLAALTRA
LAEAGFDTVYAADSNGALYPDEVAAIVGEMRRHFTGQIGFHAHDSLHLAFANALAAMRAGASWVDGTIGGVGKGGGNAAI
EVLLSELAKADSRPIDIAGFSQMPASFPHDFFPVRFKERLDNVFYGRNNLNIDKIKSLESRPSH

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI1786261, Length=270, Percent_Identity=26.6666666666667, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- ProDom:   PD005364 [H]

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like [H]

EC number: =4.1.3.39 [H]

Molecular weight: Translated: 32831; Mature: 32699

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS50991 PYR_CT ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLSNQAHCARAVTLLDCTLRDGGYLNGHDFSAPQIDAILTRLLASGVDVIEVGYFRRR
CCCCCCCHHHHHHHHHHEEEECCCCEECCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHC
NVERHPSTVIARCPPAFLASLARTQAVRYAVMVPPGDAEPADLDELAELPVSVVRFTCTD
CCCCCCCEEEECCCHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHCCHHHEEEEECC
QNLPKVLALGERAARLGLVVCANITRVSERSHSQLAALTRALAEAGFDTVYAADSNGALY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
PDEVAAIVGEMRRHFTGQIGFHAHDSLHLAFANALAAMRAGASWVDGTIGGVGKGGGNAA
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
IEVLLSELAKADSRPIDIAGFSQMPASFPHDFFPVRFKERLDNVFYGRNNLNIDKIKSLE
HHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCHHHHHHHH
SRPSH
CCCCC
>Mature Secondary Structure 
TLLSNQAHCARAVTLLDCTLRDGGYLNGHDFSAPQIDAILTRLLASGVDVIEVGYFRRR
CCCCCCHHHHHHHHHHEEEECCCCEECCCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHC
NVERHPSTVIARCPPAFLASLARTQAVRYAVMVPPGDAEPADLDELAELPVSVVRFTCTD
CCCCCCCEEEECCCHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHCCHHHEEEEECC
QNLPKVLALGERAARLGLVVCANITRVSERSHSQLAALTRALAEAGFDTVYAADSNGALY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCC
PDEVAAIVGEMRRHFTGQIGFHAHDSLHLAFANALAAMRAGASWVDGTIGGVGKGGGNAA
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHH
IEVLLSELAKADSRPIDIAGFSQMPASFPHDFFPVRFKERLDNVFYGRNNLNIDKIKSLE
HHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHEECCCCCCHHHHHHHH
SRPSH
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA