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The map label for this gene is phaZ1 [H]

Identifier: 238023980

GI number: 238023980

Start: 637346

End: 638443

Strand: Reverse

Name: phaZ1 [H]

Synonym: bglu_2g05300

Alternate gene names: 238023980

Gene position: 638443-637346 (Counterclockwise)

Preceding gene: 238023984

Following gene: 238023979

Centisome position: 22.58

GC content: 73.04

Gene sequence:

>1098_bases
ATGCCCAGGAAAAAAGCCAGCGTCTGGTTCAGCGGCCTTGATCTACTTGCCAGACTCGCGACAGTCCGGCCGAAACCGCC
GCGCATGGCGCGCAAGCCGAAGCCCAAGCCGAAACGCGCCAAGCCGCGCCATCCGGCGCGCGCCGCCCCACTGGGCCCGC
GCCACGCGCCGCCGGCGCGCGCGCCGGGCCGCTGGACCCGTTCGTTCCACTCCGCGCCGCCGCTGGCCGGACAGTTCGTC
AACCATCTCGCCTACGCGCTGTATCTGCCGGCGTCGGCAACGTCGGCGGCCGGCCTGCCGCTCCTGGTGATGCTGCACGG
CTGCCGGCAGACCGTGGAATCGTTCGCGGCCGGCACGCGCATCTGCCGGCTCGCCGAGCGCGCCGGCTTCGCGGTGCTGC
TGCCCGAGCAGGCGAAGACGGCGCACGCCCACCGCTGCTGGCATTGGCACCAGCCGCCCGCGCATTCCGAGGCGCCCGCC
ATCGCGTCGCTCGTCGCGGCGATGGTCCGCGAACACGGCTTCGACCACGAACGCATCCATCTGGCCGGCATGTCGGCGGG
CGCCGGCCTCGCCGCAGTGCTCGCCGTGCATCACCCGGCACTGTTCGCCGCGGTGGGCCTGCACTCGGGCCCGGTGTTCG
GCGCCGCGAATTCGGCGCTGAGCGCCATGCGCGTGATGCGCGACGGTAGCCGCGAGGAACCGCTGCGCCTGATCGACACA
GCCGTCGAGGTGGCCGACCATCCCGGCATGCCGGCGCTCGTGATTCACGGCGAACTCGACGCGGTGGTCGCCAACCGCAA
CGCCACGCAGCTCGGCACCGAATTCGCGCGGCTCAATCGCCTGCTGGACGCGCAAGGCGGGCTGCGCGCCGGCGAGCGCC
GCACCGGGCGGCGCGACGACGTCGAATACCACGACTACCTGAAAGCGGGCCGGCTGGTGGTGCGGGTGGCCATCGTGCGC
GGCCTCGGCCACGCCTGGAGCGGCGGCGATCCGCGCGAGCCGTTCCACGCCGATTGCGGCCCCGACGCGAGCCGCATGAT
GTGGAATTTTTTTCGGCGTCAGCGGCGCACCACGGCCGGCGCGGCCGCGCCGGCATGA

Upstream 100 bases:

>100_bases
TAGCATGGGAAAGACTCTCGATCCGGCACCGTGCCGACTGCCGCGGCGCGGCCGCGCCCCTTCGACTTTTTCACCCGCTT
CGATCCTCTCGTCCCCGCCC

Downstream 100 bases:

>100_bases
CCCCGCGGCCCGTGCCGGTGCGTCAATCGCGCATGGTGCTGCGCTGCCGCCTGGCGGCTTTGACACCCGTCATCCAACTC
GAACAATTACGATTGATGGT

Product: PHB depolymerase family esterase

Products: NA

Alternate protein names: PHA depolymerase; PHB depolymerase [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MPRKKASVWFSGLDLLARLATVRPKPPRMARKPKPKPKRAKPRHPARAAPLGPRHAPPARAPGRWTRSFHSAPPLAGQFV
NHLAYALYLPASATSAAGLPLLVMLHGCRQTVESFAAGTRICRLAERAGFAVLLPEQAKTAHAHRCWHWHQPPAHSEAPA
IASLVAAMVREHGFDHERIHLAGMSAGAGLAAVLAVHHPALFAAVGLHSGPVFGAANSALSAMRVMRDGSREEPLRLIDT
AVEVADHPGMPALVIHGELDAVVANRNATQLGTEFARLNRLLDAQGGLRAGERRTGRRDDVEYHDYLKAGRLVVRVAIVR
GLGHAWSGGDPREPFHADCGPDASRMMWNFFRRQRRTTAGAAAPA

Sequences:

>Translated_365_residues
MPRKKASVWFSGLDLLARLATVRPKPPRMARKPKPKPKRAKPRHPARAAPLGPRHAPPARAPGRWTRSFHSAPPLAGQFV
NHLAYALYLPASATSAAGLPLLVMLHGCRQTVESFAAGTRICRLAERAGFAVLLPEQAKTAHAHRCWHWHQPPAHSEAPA
IASLVAAMVREHGFDHERIHLAGMSAGAGLAAVLAVHHPALFAAVGLHSGPVFGAANSALSAMRVMRDGSREEPLRLIDT
AVEVADHPGMPALVIHGELDAVVANRNATQLGTEFARLNRLLDAQGGLRAGERRTGRRDDVEYHDYLKAGRLVVRVAIVR
GLGHAWSGGDPREPFHADCGPDASRMMWNFFRRQRRTTAGAAAPA
>Mature_364_residues
PRKKASVWFSGLDLLARLATVRPKPPRMARKPKPKPKRAKPRHPARAAPLGPRHAPPARAPGRWTRSFHSAPPLAGQFVN
HLAYALYLPASATSAAGLPLLVMLHGCRQTVESFAAGTRICRLAERAGFAVLLPEQAKTAHAHRCWHWHQPPAHSEAPAI
ASLVAAMVREHGFDHERIHLAGMSAGAGLAAVLAVHHPALFAAVGLHSGPVFGAANSALSAMRVMRDGSREEPLRLIDTA
VEVADHPGMPALVIHGELDAVVANRNATQLGTEFARLNRLLDAQGGLRAGERRTGRRDDVEYHDYLKAGRLVVRVAIVRG
LGHAWSGGDPREPFHADCGPDASRMMWNFFRRQRRTTAGAAAPA

Specific function: Specific for poly(hydroxyalkanoic acid) consisting of monomers of four or five carbon atoms and for P- nitrophenylbutyrate as substrates [H]

COG id: COG3509

COG function: function code Q; Poly(3-hydroxybutyrate) depolymerase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Lipase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010126 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 39442; Mature: 39311

Theoretical pI: Translated: 11.68; Mature: 11.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRKKASVWFSGLDLLARLATVRPKPPRMARKPKPKPKRAKPRHPARAAPLGPRHAPPAR
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APGRWTRSFHSAPPLAGQFVNHLAYALYLPASATSAAGLPLLVMLHGCRQTVESFAAGTR
CCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ICRLAERAGFAVLLPEQAKTAHAHRCWHWHQPPAHSEAPAIASLVAAMVREHGFDHERIH
HHHHHHHCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
LAGMSAGAGLAAVLAVHHPALFAAVGLHSGPVFGAANSALSAMRVMRDGSREEPLRLIDT
EECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
AVEVADHPGMPALVIHGELDAVVANRNATQLGTEFARLNRLLDAQGGLRAGERRTGRRDD
HHHHHCCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCC
VEYHDYLKAGRLVVRVAIVRGLGHAWSGGDPREPFHADCGPDASRMMWNFFRRQRRTTAG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
AAAPA
CCCCC
>Mature Secondary Structure 
PRKKASVWFSGLDLLARLATVRPKPPRMARKPKPKPKRAKPRHPARAAPLGPRHAPPAR
CCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APGRWTRSFHSAPPLAGQFVNHLAYALYLPASATSAAGLPLLVMLHGCRQTVESFAAGTR
CCCHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ICRLAERAGFAVLLPEQAKTAHAHRCWHWHQPPAHSEAPAIASLVAAMVREHGFDHERIH
HHHHHHHCCEEEEECCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCEEE
LAGMSAGAGLAAVLAVHHPALFAAVGLHSGPVFGAANSALSAMRVMRDGSREEPLRLIDT
EECCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHH
AVEVADHPGMPALVIHGELDAVVANRNATQLGTEFARLNRLLDAQGGLRAGERRTGRRDD
HHHHHCCCCCCEEEEECCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCC
VEYHDYLKAGRLVVRVAIVRGLGHAWSGGDPREPFHADCGPDASRMMWNFFRRQRRTTAG
CHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
AAAPA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8269961 [H]