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The map label for this gene is rbsB [H]

Identifier: 238023896

GI number: 238023896

Start: 523942

End: 524883

Strand: Reverse

Name: rbsB [H]

Synonym: bglu_2g04350

Alternate gene names: 238023896

Gene position: 524883-523942 (Counterclockwise)

Preceding gene: 238023897

Following gene: 238023895

Centisome position: 18.56

GC content: 65.18

Gene sequence:

>942_bases
ATGTTCAAGCAAAACGCCACGCTGACCGTCCTCGCCTGCGCACTCGCCTTCGGCGCAGGCGCCGCCCATGCCGCCGACAA
GCCGCTGAAATCGATCGGCGTCACGGTGGGCTCGCTCGGCAACCCGTACTTCGTGACGATCGTCAAGGGCGCCGAAGCAC
GCGCCAGGCAGATCAACCCGAACGCGAAAGTCATCGCGGTTTCGGCCGATTACGATCTCAACAAGCAGTTCACCCAGATC
GACAACTTCATCTCCGCGCATGTCGACATGATCCTGCTCAACGCCACCGATCCGAAGGCGATCGAGCCGGCGGTGAAGAA
GGCGCAGGCGGCCGGCATCACGGTGGTGGCGGTGGACGTCTCGGCCGCGGGCGCGAACGCGACCGTGCAGACCAACAACG
TGAAGGCCGGCGAGCTGGCCTGCGATTACCTGGCGAAGAAGCTCAACGGCAAGGGCAACGTCGTCATCGAGAACGGCCCG
CAGGTGTCGGCCGTGATCGACCGCGTCAACGGCTGCAAGACCGAGCTGGCGAAGCACCCCGGCATCAAGCTGCTGTCGAG
CGACCAGGACGGCAAGGGCTCGCGTGAAGGCGGCATGAACGCGATGCAGGGCTACCTGACGCGCTTCCCGAAGCTCGACG
GCGTGTTCACCATCAACGACCCGCAGGCAATCGGCAGCGACCTCGCGGCCAAGCAGCTGAACCGCCCGAACATCGTGATC
ACGTCGGTGGACGGCGCGCCCGACATGGAAGTGGCGCTGAAGACGAACACGCTGATCCAGGCGTCGTCGAGCCAGGACCC
GTGGGCGATGGCGCAGCAGGCCGTGACGGTCGGCTACGGGATCATGAACGGCAAGCCGCCGGCCAACCCGATGATCCTGA
TCGAGCCGACGCTGGTCACGCGCGACAACGTGAAGGACTACAAGGGCTGGAGCTCGCACTGA

Upstream 100 bases:

>100_bases
GAATCGGCCCGCACCTGAGCGGCCGCCACCGCTCCACGCAAGGGTGCGGGCAGGTCCTCCCGCACCCCGCCGCCAACGGA
CCTTCCGGAGACACAACGAC

Downstream 100 bases:

>100_bases
GCGACGCCGGCGCGGCGCCCGCCGCACCGCCGGTCGCGCGCATCGCGCGCGTGACGCCGCCGGCGGGCGCCCGCTCGGGG
GCCGCGCCCGCCGCCGCGCA

Product: Periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MFKQNATLTVLACALAFGAGAAHAADKPLKSIGVTVGSLGNPYFVTIVKGAEARARQINPNAKVIAVSADYDLNKQFTQI
DNFISAHVDMILLNATDPKAIEPAVKKAQAAGITVVAVDVSAAGANATVQTNNVKAGELACDYLAKKLNGKGNVVIENGP
QVSAVIDRVNGCKTELAKHPGIKLLSSDQDGKGSREGGMNAMQGYLTRFPKLDGVFTINDPQAIGSDLAAKQLNRPNIVI
TSVDGAPDMEVALKTNTLIQASSSQDPWAMAQQAVTVGYGIMNGKPPANPMILIEPTLVTRDNVKDYKGWSSH

Sequences:

>Translated_313_residues
MFKQNATLTVLACALAFGAGAAHAADKPLKSIGVTVGSLGNPYFVTIVKGAEARARQINPNAKVIAVSADYDLNKQFTQI
DNFISAHVDMILLNATDPKAIEPAVKKAQAAGITVVAVDVSAAGANATVQTNNVKAGELACDYLAKKLNGKGNVVIENGP
QVSAVIDRVNGCKTELAKHPGIKLLSSDQDGKGSREGGMNAMQGYLTRFPKLDGVFTINDPQAIGSDLAAKQLNRPNIVI
TSVDGAPDMEVALKTNTLIQASSSQDPWAMAQQAVTVGYGIMNGKPPANPMILIEPTLVTRDNVKDYKGWSSH
>Mature_313_residues
MFKQNATLTVLACALAFGAGAAHAADKPLKSIGVTVGSLGNPYFVTIVKGAEARARQINPNAKVIAVSADYDLNKQFTQI
DNFISAHVDMILLNATDPKAIEPAVKKAQAAGITVVAVDVSAAGANATVQTNNVKAGELACDYLAKKLNGKGNVVIENGP
QVSAVIDRVNGCKTELAKHPGIKLLSSDQDGKGSREGGMNAMQGYLTRFPKLDGVFTINDPQAIGSDLAAKQLNRPNIVI
TSVDGAPDMEVALKTNTLIQASSSQDPWAMAQQAVTVGYGIMNGKPPANPMILIEPTLVTRDNVKDYKGWSSH

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=283, Percent_Identity=29.6819787985866, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1790674, Length=191, Percent_Identity=29.8429319371728, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32836; Mature: 32836

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKQNATLTVLACALAFGAGAAHAADKPLKSIGVTVGSLGNPYFVTIVKGAEARARQINP
CCCCCCHHHHHHHHHHHCCCCCHHCCCCHHHHCEEECCCCCCEEEEEEECCCCHHHCCCC
NAKVIAVSADYDLNKQFTQIDNFISAHVDMILLNATDPKAIEPAVKKAQAAGITVVAVDV
CCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCEEEEEEEE
SAAGANATVQTNNVKAGELACDYLAKKLNGKGNVVIENGPQVSAVIDRVNGCKTELAKHP
ECCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCHHHHHHCCC
GIKLLSSDQDGKGSREGGMNAMQGYLTRFPKLDGVFTINDPQAIGSDLAAKQLNRPNIVI
CCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEE
TSVDGAPDMEVALKTNTLIQASSSQDPWAMAQQAVTVGYGIMNGKPPANPMILIEPTLVT
EECCCCCCCEEEEEECEEEEECCCCCHHHHHHHHHHEEEEECCCCCCCCCEEEEECCEEE
RDNVKDYKGWSSH
CCCCHHCCCCCCC
>Mature Secondary Structure
MFKQNATLTVLACALAFGAGAAHAADKPLKSIGVTVGSLGNPYFVTIVKGAEARARQINP
CCCCCCHHHHHHHHHHHCCCCCHHCCCCHHHHCEEECCCCCCEEEEEEECCCCHHHCCCC
NAKVIAVSADYDLNKQFTQIDNFISAHVDMILLNATDPKAIEPAVKKAQAAGITVVAVDV
CCEEEEEECCCCCCHHHHHHHHHHHHCEEEEEEECCCCHHHHHHHHHHHHCCEEEEEEEE
SAAGANATVQTNNVKAGELACDYLAKKLNGKGNVVIENGPQVSAVIDRVNGCKTELAKHP
ECCCCCCEEEECCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHCCHHHHHHCCC
GIKLLSSDQDGKGSREGGMNAMQGYLTRFPKLDGVFTINDPQAIGSDLAAKQLNRPNIVI
CCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCCCEEE
TSVDGAPDMEVALKTNTLIQASSSQDPWAMAQQAVTVGYGIMNGKPPANPMILIEPTLVT
EECCCCCCCEEEEEECEEEEECCCCCHHHHHHHHHHEEEEECCCCCCCCCEEEEECCEEE
RDNVKDYKGWSSH
CCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]