Click here to switch to the map view.

The map label for this gene is mnmC [H]

Identifier: 238023841

GI number: 238023841

Start: 451018

End: 452145

Strand: Direct

Name: mnmC [H]

Synonym: bglu_2g03800

Alternate gene names: 238023841

Gene position: 451018-452145 (Clockwise)

Preceding gene: 238023840

Following gene: 238023842

Centisome position: 15.95

GC content: 75.89

Gene sequence:

>1128_bases
ATGGCACGCGCGGACGTGATCGTGATCGGCGCCGGCATCGTCGGCGCGGCCTGCGCGGCCGAGCTGGCCGCGCGCGGCCT
CGACGTGGAGGTGCTCGACGCGGGCGGCATCGGCGGCGGCGCGACCGCGGCGGGCATGGGCCATCTGGTCGTGATGAACG
ACACGCCGGCCGAACTCGCGCTGTCGCGCCGCTCGCTCGCACGGTGGCGCGCGCTCGCGCCGCAGTTGCGCCAGTGCGAT
GCCTATCTGCGCTGCGGCACGCTCTGGGTGGCCGCCGACGACGAGGAACTGGAGGCCGCGCGCGCGATGCGCGACGGCTA
CGCCGCGGCCGGCGTCTCGGCCGAGGTGCTCGATGCGGCCGCGCTGGGCGCGCGCGAGCCGATGCTCGCGGCGGGGCTCG
CGGGCGGGCTGCTGGTGGACGGCGACGGAATCGTCTACGCGCCGTCGGCGGCCGAATGGCTGCTCAGGCACTCGCCCGGC
GCCGCGCGCATCCGGCTGCGCCTGCGCTGCGCCGCCGTCGCGCTCGACGGCACGACGGTGCGGCTGGCCGGCGGCGAGAC
GCTGGACGCGGCGCAGGTGGTCGTCGCCAACGGGCTCGCCGCGGCCCGGCTCGTCGACGGCGTCGCGCTGCAGCCGAAGA
AGGGCCACCTGCTGATCACCGACCGCTATCCGGGCGCGATCACGCACCAGCTGCTCGAACTCGGCTATATCAAGAGCGCG
CATCATGCCCAGGGCACCTCGGTGGCGTTCAACGCGCAGCCGCGCCCCACCGGGCAGATCCTGATCGGCTCGTCGCGCCA
GTTCGGCACCACCGATCCGGCCGTCGAGATGCCGGTGCTGGCGCGCATGCTGGCGCGCGCGCAGCAGTACCTGCCGGGGC
TCGCGGACCTGAGCGCGCTGCGTGCCTGGACCGGCTTTCGCGCCGCGACGCCGGACGGGCTGCCGCTGATCGGCCCGGTG
GCGGAGCGGCCCGGCGTATGGCTCGCCACCGGCCACGAAGGGCTCGGCGTGACGACCTCGCTGGCCACGGCGGAGCTGAT
CGCCGCCCAGCTGGCGGGCGGCGAGCCGCCGATTCCTCCCGAACCCTACAGCCCGTCCCGGCCGCTTGCCGGAGCCGCCC
ATGCCTGA

Upstream 100 bases:

>100_bases
CGATCATGCCCACCCTGACCGGGCAGGCCTACGTGATGGCCGAGGGGCGGCTCTGCTTCGACGCGCGCGATCCGTTCGCG
AACGGGATCCGGCTCGGCTG

Downstream 100 bases:

>100_bases
TCGCAAGCCCATCCGGACCGCTGCCGCCGCGCTCGGCGTGACCATCGACGGGCGCGCCTGCCGCGTGCCCGAGGGCGCCA
CCGTCGCGGCCGCGCTCGCG

Product: putative oxidoreductase

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MARADVIVIGAGIVGAACAAELAARGLDVEVLDAGGIGGGATAAGMGHLVVMNDTPAELALSRRSLARWRALAPQLRQCD
AYLRCGTLWVAADDEELEAARAMRDGYAAAGVSAEVLDAAALGAREPMLAAGLAGGLLVDGDGIVYAPSAAEWLLRHSPG
AARIRLRLRCAAVALDGTTVRLAGGETLDAAQVVVANGLAAARLVDGVALQPKKGHLLITDRYPGAITHQLLELGYIKSA
HHAQGTSVAFNAQPRPTGQILIGSSRQFGTTDPAVEMPVLARMLARAQQYLPGLADLSALRAWTGFRAATPDGLPLIGPV
AERPGVWLATGHEGLGVTTSLATAELIAAQLAGGEPPIPPEPYSPSRPLAGAAHA

Sequences:

>Translated_375_residues
MARADVIVIGAGIVGAACAAELAARGLDVEVLDAGGIGGGATAAGMGHLVVMNDTPAELALSRRSLARWRALAPQLRQCD
AYLRCGTLWVAADDEELEAARAMRDGYAAAGVSAEVLDAAALGAREPMLAAGLAGGLLVDGDGIVYAPSAAEWLLRHSPG
AARIRLRLRCAAVALDGTTVRLAGGETLDAAQVVVANGLAAARLVDGVALQPKKGHLLITDRYPGAITHQLLELGYIKSA
HHAQGTSVAFNAQPRPTGQILIGSSRQFGTTDPAVEMPVLARMLARAQQYLPGLADLSALRAWTGFRAATPDGLPLIGPV
AERPGVWLATGHEGLGVTTSLATAELIAAQLAGGEPPIPPEPYSPSRPLAGAAHA
>Mature_374_residues
ARADVIVIGAGIVGAACAAELAARGLDVEVLDAGGIGGGATAAGMGHLVVMNDTPAELALSRRSLARWRALAPQLRQCDA
YLRCGTLWVAADDEELEAARAMRDGYAAAGVSAEVLDAAALGAREPMLAAGLAGGLLVDGDGIVYAPSAAEWLLRHSPGA
ARIRLRLRCAAVALDGTTVRLAGGETLDAAQVVVANGLAAARLVDGVALQPKKGHLLITDRYPGAITHQLLELGYIKSAH
HAQGTSVAFNAQPRPTGQILIGSSRQFGTTDPAVEMPVLARMLARAQQYLPGLADLSALRAWTGFRAATPDGLPLIGPVA
ERPGVWLATGHEGLGVTTSLATAELIAAQLAGGEPPIPPEPYSPSRPLAGAAHA

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610 [H]

Pfam domain/function: PF01266 DAO; PF05430 DUF752 [H]

EC number: =2.1.1.61 [H]

Molecular weight: Translated: 38301; Mature: 38170

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARADVIVIGAGIVGAACAAELAARGLDVEVLDAGGIGGGATAAGMGHLVVMNDTPAELA
CCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHCCCCEEEEECCCCHHHH
LSRRSLARWRALAPQLRQCDAYLRCGTLWVAADDEELEAARAMRDGYAAAGVSAEVLDAA
HHHHHHHHHHHHHHHHHHHHHHEEECEEEEEECHHHHHHHHHHHCCHHHCCCCHHHHHHH
ALGAREPMLAAGLAGGLLVDGDGIVYAPSAAEWLLRHSPGAARIRLRLRCAAVALDGTTV
HHCCCCCHHHHHCCCCEEECCCCEEECCCHHHHHHHCCCCCEEEEEEEEEEEEEECCCEE
RLAGGETLDAAQVVVANGLAAARLVDGVALQPKKGHLLITDRYPGAITHQLLELGYIKSA
EEECCCCCCHHHHHHHCCHHHHHHHCCEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCC
HHAQGTSVAFNAQPRPTGQILIGSSRQFGTTDPAVEMPVLARMLARAQQYLPGLADLSAL
CCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
RAWTGFRAATPDGLPLIGPVAERPGVWLATGHEGLGVTTSLATAELIAAQLAGGEPPIPP
HHHHCCCCCCCCCCCEECCCCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHCCCCCCCCC
EPYSPSRPLAGAAHA
CCCCCCCCCCCCCCC
>Mature Secondary Structure 
ARADVIVIGAGIVGAACAAELAARGLDVEVLDAGGIGGGATAAGMGHLVVMNDTPAELA
CCCCEEEEECCHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHCCCCEEEEECCCCHHHH
LSRRSLARWRALAPQLRQCDAYLRCGTLWVAADDEELEAARAMRDGYAAAGVSAEVLDAA
HHHHHHHHHHHHHHHHHHHHHHEEECEEEEEECHHHHHHHHHHHCCHHHCCCCHHHHHHH
ALGAREPMLAAGLAGGLLVDGDGIVYAPSAAEWLLRHSPGAARIRLRLRCAAVALDGTTV
HHCCCCCHHHHHCCCCEEECCCCEEECCCHHHHHHHCCCCCEEEEEEEEEEEEEECCCEE
RLAGGETLDAAQVVVANGLAAARLVDGVALQPKKGHLLITDRYPGAITHQLLELGYIKSA
EEECCCCCCHHHHHHHCCHHHHHHHCCEEECCCCCCEEEEECCCCHHHHHHHHHHHHCCC
HHAQGTSVAFNAQPRPTGQILIGSSRQFGTTDPAVEMPVLARMLARAQQYLPGLADLSAL
CCCCCCEEEEECCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHH
RAWTGFRAATPDGLPLIGPVAERPGVWLATGHEGLGVTTSLATAELIAAQLAGGEPPIPP
HHHHCCCCCCCCCCCEECCCCCCCCEEEEECCCCCCEEHHHHHHHHHHHHHCCCCCCCCC
EPYSPSRPLAGAAHA
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA