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The map label for this gene is 238023830

Identifier: 238023830

GI number: 238023830

Start: 436055

End: 437476

Strand: Reverse

Name: 238023830

Synonym: bglu_2g03680

Alternate gene names: NA

Gene position: 437476-436055 (Counterclockwise)

Preceding gene: 238023831

Following gene: 238023829

Centisome position: 15.47

GC content: 65.68

Gene sequence:

>1422_bases
ATGAAAACCCTGTTCCTGCAAGCGCCGTCCTACGACGGCTTCGACGGCGGCGCCGGCTCGCGCTACCAGGCCCGGCGCGA
GATCCGCTCGTTCTGGTATCCCACCTGGCTGGCCCAGCCCGCCGCGCTCGTGCCCGGCAGCCGCGTGCTCGACGCCCCCG
CCGACGGCCTGTCGGTGCAGGCCACGCTCGAGATCGCCGCCGGCTACGACCTCGTCATCATCCACACCAGCACGCCCTCG
TTTCCCACCGACGCCCTGTTCGCCCAGGACCTCAAGCAGCGCAAGCGCTCGATCGTGATCGGCATGGTCGGCGCCAAGGT
CGCCGTCGATCCCCACCATTGCCTGCTCGCCTCCGAGGCGATCGATTTCGTCTGCCGCGAGGAGTTCGACTACACCTGCC
GCGAGCTGGCCGAAGGCAAGCCGTTCGCCGAGATCCTCGGCCTGAGCTACCGCACCGCCGACGGCGCGATCGAGCACAAC
GGCCCGCGCCCGATGATCGAGGACATGGACGCGCTGCCGTTCGTGGCCCCCGTCTATCAGCGCGACCTCAAGATCCGCAA
CTACTTCATCGGCTATCTCGACTACCCCTACGTCTCGATCTACACCGGGCGCGGCTGCCGCTCCAAATGCACCTTCTGCC
TGTGGCCGCAGACCGTGGGCGGCCACCGCTACCGCGTGCGCTCGGTCGAGAACGTGCTGGCCGAGGTGAAGTGGATTCGC
GACAACATGCCGGAGGTGAAAGAGATCATGTTCGACGACGACACCTTCACCGACTTCAAGCCGCGCGTGGAGGAAATCGC
GCGCGGGCTCGGCAGGCTGGGCGTGAGCTGGTCGTGCAACGCCAAGGCGAACGTGCCGTACTCGACGCTGAAGATCATGA
AGGACAACGGCCTGCGCCTGCTGCTGGTGGGCTACGAGTCGGGCGACGACCAGATCCTGCTGAACATCAAGAAGGGCCTG
CGCACCGACATCGCGCGCCGCTTCAGCGAGGACTGCAGGAAGCTGGGCATCAAGATCCACGGCACCTTCATCCTCGGGCT
GCCGGGCGAGACCAAGGACACCATCCGCAAGACCATCGCCTACGCGAAGGAGATCAATCCGCACACCATCCAGGTGTCGC
TGGCCGCGCCCTATCCGGGCACCAAGCTCTACGAGCAGGCGGTGGAAAACGGCTGGATGGAGGAGAACCGGACCATCAAC
CTGGTCAGCAAGGAGGGCGTGCAGCTGGCGGCGATCGGCTATCCGCATCTGTCGCGGGAAGACATCTATCACGAGCTGGA
GCACTTCTATCGCGCGTTCTACTTCCGGCCGTCGAAGATCTGGGAGATCGTGCGCGAGATGCTGTCGAGCTGGGACATGA
CGAAGCGCCGCCTGCGCGAAGGCGTCGAGTTCTTCCGCTTCCTGCGCGCGCACGAGGCGTGA

Upstream 100 bases:

>100_bases
TCGTGACCAGCAGCGGCCCGACGAACGACTGCGAACCCGCCATCCGACGCTGCGGGTGCCGGCCGCCGGGGCCGGCGCCG
CGCCATCTCTTCCCGACCCG

Downstream 100 bases:

>100_bases
TGCAGGGGCTGCGCTCGTGGACGCGCGGCCAGGCCGGCCGCGCCTTCTCCATGTCGCTCGCCGTCGGCGCCGCGATCGTG
CTGAGCGACGCGGCGCGCCA

Product: Radical SAM domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 473

Protein sequence:

>473_residues
MKTLFLQAPSYDGFDGGAGSRYQARREIRSFWYPTWLAQPAALVPGSRVLDAPADGLSVQATLEIAAGYDLVIIHTSTPS
FPTDALFAQDLKQRKRSIVIGMVGAKVAVDPHHCLLASEAIDFVCREEFDYTCRELAEGKPFAEILGLSYRTADGAIEHN
GPRPMIEDMDALPFVAPVYQRDLKIRNYFIGYLDYPYVSIYTGRGCRSKCTFCLWPQTVGGHRYRVRSVENVLAEVKWIR
DNMPEVKEIMFDDDTFTDFKPRVEEIARGLGRLGVSWSCNAKANVPYSTLKIMKDNGLRLLLVGYESGDDQILLNIKKGL
RTDIARRFSEDCRKLGIKIHGTFILGLPGETKDTIRKTIAYAKEINPHTIQVSLAAPYPGTKLYEQAVENGWMEENRTIN
LVSKEGVQLAAIGYPHLSREDIYHELEHFYRAFYFRPSKIWEIVREMLSSWDMTKRRLREGVEFFRFLRAHEA

Sequences:

>Translated_473_residues
MKTLFLQAPSYDGFDGGAGSRYQARREIRSFWYPTWLAQPAALVPGSRVLDAPADGLSVQATLEIAAGYDLVIIHTSTPS
FPTDALFAQDLKQRKRSIVIGMVGAKVAVDPHHCLLASEAIDFVCREEFDYTCRELAEGKPFAEILGLSYRTADGAIEHN
GPRPMIEDMDALPFVAPVYQRDLKIRNYFIGYLDYPYVSIYTGRGCRSKCTFCLWPQTVGGHRYRVRSVENVLAEVKWIR
DNMPEVKEIMFDDDTFTDFKPRVEEIARGLGRLGVSWSCNAKANVPYSTLKIMKDNGLRLLLVGYESGDDQILLNIKKGL
RTDIARRFSEDCRKLGIKIHGTFILGLPGETKDTIRKTIAYAKEINPHTIQVSLAAPYPGTKLYEQAVENGWMEENRTIN
LVSKEGVQLAAIGYPHLSREDIYHELEHFYRAFYFRPSKIWEIVREMLSSWDMTKRRLREGVEFFRFLRAHEA
>Mature_473_residues
MKTLFLQAPSYDGFDGGAGSRYQARREIRSFWYPTWLAQPAALVPGSRVLDAPADGLSVQATLEIAAGYDLVIIHTSTPS
FPTDALFAQDLKQRKRSIVIGMVGAKVAVDPHHCLLASEAIDFVCREEFDYTCRELAEGKPFAEILGLSYRTADGAIEHN
GPRPMIEDMDALPFVAPVYQRDLKIRNYFIGYLDYPYVSIYTGRGCRSKCTFCLWPQTVGGHRYRVRSVENVLAEVKWIR
DNMPEVKEIMFDDDTFTDFKPRVEEIARGLGRLGVSWSCNAKANVPYSTLKIMKDNGLRLLLVGYESGDDQILLNIKKGL
RTDIARRFSEDCRKLGIKIHGTFILGLPGETKDTIRKTIAYAKEINPHTIQVSLAAPYPGTKLYEQAVENGWMEENRTIN
LVSKEGVQLAAIGYPHLSREDIYHELEHFYRAFYFRPSKIWEIVREMLSSWDMTKRRLREGVEFFRFLRAHEA

Specific function: Unknown

COG id: COG1032

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 B12-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR007197 [H]

Pfam domain/function: PF02310 B12-binding; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53919; Mature: 53919

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLFLQAPSYDGFDGGAGSRYQARREIRSFWYPTWLAQPAALVPGSRVLDAPADGLSVQ
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCEECCCCCCCEEE
ATLEIAAGYDLVIIHTSTPSFPTDALFAQDLKQRKRSIVIGMVGAKVAVDPHHCLLASEA
EEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHEEEEEEECCEEEECCHHHHHHHHH
IDFVCREEFDYTCRELAEGKPFAEILGLSYRTADGAIEHNGPRPMIEDMDALPFVAPVYQ
HHHHHHHHCCHHHHHHHCCCCHHHHHCCEEECCCCCCCCCCCCCHHHCCCCCCCHHHHHH
RDLKIRNYFIGYLDYPYVSIYTGRGCRSKCTFCLWPQTVGGHRYRVRSVENVLAEVKWIR
HHHEEEHEEEEEECCCEEEEEECCCCCCCCEEEECCCCCCCCEEEHHHHHHHHHHHHHHH
DNMPEVKEIMFDDDTFTDFKPRVEEIARGLGRLGVSWSCNAKANVPYSTLKIMKDNGLRL
CCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCHHHEEEEECCCCEE
LLVGYESGDDQILLNIKKGLRTDIARRFSEDCRKLGIKIHGTFILGLPGETKDTIRKTIA
EEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHHHHHHH
YAKEINPHTIQVSLAAPYPGTKLYEQAVENGWMEENRTINLVSKEGVQLAAIGYPHLSRE
HHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEECCCCCCHH
DIYHELEHFYRAFYFRPSKIWEIVREMLSSWDMTKRRLREGVEFFRFLRAHEA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTLFLQAPSYDGFDGGAGSRYQARREIRSFWYPTWLAQPAALVPGSRVLDAPADGLSVQ
CCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCHHHHCCCCCCCCCCCEECCCCCCCEEE
ATLEIAAGYDLVIIHTSTPSFPTDALFAQDLKQRKRSIVIGMVGAKVAVDPHHCLLASEA
EEEEEECCCEEEEEEECCCCCCCHHHHHHHHHHHHHEEEEEEECCEEEECCHHHHHHHHH
IDFVCREEFDYTCRELAEGKPFAEILGLSYRTADGAIEHNGPRPMIEDMDALPFVAPVYQ
HHHHHHHHCCHHHHHHHCCCCHHHHHCCEEECCCCCCCCCCCCCHHHCCCCCCCHHHHHH
RDLKIRNYFIGYLDYPYVSIYTGRGCRSKCTFCLWPQTVGGHRYRVRSVENVLAEVKWIR
HHHEEEHEEEEEECCCEEEEEECCCCCCCCEEEECCCCCCCCEEEHHHHHHHHHHHHHHH
DNMPEVKEIMFDDDTFTDFKPRVEEIARGLGRLGVSWSCNAKANVPYSTLKIMKDNGLRL
CCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCEECCCCCCCCHHHEEEEECCCCEE
LLVGYESGDDQILLNIKKGLRTDIARRFSEDCRKLGIKIHGTFILGLPGETKDTIRKTIA
EEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHCCEEEEEEEEEECCCCCHHHHHHHHH
YAKEINPHTIQVSLAAPYPGTKLYEQAVENGWMEENRTINLVSKEGVQLAAIGYPHLSRE
HHHCCCCEEEEEEEECCCCCHHHHHHHHHCCCCCCCCEEEEEECCCEEEEEECCCCCCHH
DIYHELEHFYRAFYFRPSKIWEIVREMLSSWDMTKRRLREGVEFFRFLRAHEA
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]