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The map label for this gene is mdtE [H]

Identifier: 238023816

GI number: 238023816

Start: 416708

End: 417922

Strand: Direct

Name: mdtE [H]

Synonym: bglu_2g03540

Alternate gene names: 238023816

Gene position: 416708-417922 (Clockwise)

Preceding gene: 238023815

Following gene: 238023817

Centisome position: 14.74

GC content: 73.09

Gene sequence:

>1215_bases
ATGTCCATCCTAGCTACTCCCCGCTCTCGCCTCGCGGCGGCCGCGTTGGCCGTCGCGCTCGCCGTCGGCGGCGGCGCGTT
CGGCGTGATCCGCGCCAGCGCGAGCGCCCCCGCGGCCCAGGCCGCGCCGCTGCAGGAAGTGGACGTCGCGACCGTGCTGT
CGAAGACGATCACCGACTGGCAGACCTACTCGGGACGGCTCGAGGCGATCGAGCGCGTCGACGTGCGCCCGCTCGTGTCG
GGCACCATCGTGTCGGTGAACTTCAAGGACGGCGCGCTGGTGAAGAAGGGCGACGTGCTGTTCGTGATCGACCCGCGCCC
ATACCAGGCCGAAGTCGACCGCGCGGCCGCGCAGCTGGCCGCCGCCCAGGCGCGCGACGGCTACGCGCAGACCGACTGGC
AGCGCGCGCAGCGCCTGCTCGGCGACAACGCGATCGCCCGGCGCGACTATGACGAGAAGCAGAACGCCGCGCGCGAGGCG
AGCGCCAACGTGAAGGCCGCGGCCGCCGCGCTGGAGGCCGCCAGGATCAACCTCGGCTACACGCGCATCGTCGCGCCGAT
CTCGGGCCGCGCCTCGCGCGCCGAGATCACGCTCGGCAACGTGGTGTCGGCCGGCGCCACGGCCGCGCCGCTCACCACGC
TGGTCTCGGTGTCGCCCATCTATGCCTCGTTCGACGCCGACGAGCAGACCTACCTGCGCTACATCGGCGCCGTGCGCGAC
GGCCATCGCGCGCCGGTGGAGCTTGGTCTGGCCAACGAGAGCGGCTACTCGCGCAGCGGCGTGATCGACTCGGTGGACAA
CCGGCTCGACACCTCGTCGGGCACCATCCGCGTGCGCGCGCGCTTCGACAACGCCGACGGCACGCTGGTGCCGGGCCTCT
ACGCGCGCCTCAAGGTGGACGGCAGCGCACCGCATCCGGCCCTGCTGGTGGACGATGCGGCGATCAATACCGACCAGGAC
AAGAAGTTCGTGTTCGTGGTGGACGCGCAGGGCAAGGTCGCCTACCGCGAGGTGCGGATCGGCGGGCTGCACGGCAACCA
GCGCGTGATCGCGGGCGGGCTGCAGGCGAGCGACCGGGTGGTGGTGAACGGCACGCAGCGCGTGCGCCCGGGCGAACCGG
TGAAGGTCCACCTGGTGCCGATGACGGGCGGCGGCGGCGACGCGGCGGTGCCCGAGGCGGCGGCCTCGCAGGCCGCCGGC
AGCGCCGCGTCGTGA

Upstream 100 bases:

>100_bases
ACGCGCCCGCGCTGCAGGAGGCCGCGCGCTTCCTGAGCTGCCGGTTCCAGGCCCACGCCAACGACTGATTCCCGCCATTC
CAACGCAACCGGAGATCTTC

Downstream 100 bases:

>100_bases
CGGGCGCGCCTGGCTCCCACTCGCGCACACTCAGACAGAGCTTTCCATGAACCTATCCAAATTCTTCATCGATCGTCCGA
TCTTTGCAGGAGTCCTATCC

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MSILATPRSRLAAAALAVALAVGGGAFGVIRASASAPAAQAAPLQEVDVATVLSKTITDWQTYSGRLEAIERVDVRPLVS
GTIVSVNFKDGALVKKGDVLFVIDPRPYQAEVDRAAAQLAAAQARDGYAQTDWQRAQRLLGDNAIARRDYDEKQNAAREA
SANVKAAAAALEAARINLGYTRIVAPISGRASRAEITLGNVVSAGATAAPLTTLVSVSPIYASFDADEQTYLRYIGAVRD
GHRAPVELGLANESGYSRSGVIDSVDNRLDTSSGTIRVRARFDNADGTLVPGLYARLKVDGSAPHPALLVDDAAINTDQD
KKFVFVVDAQGKVAYREVRIGGLHGNQRVIAGGLQASDRVVVNGTQRVRPGEPVKVHLVPMTGGGGDAAVPEAAASQAAG
SAAS

Sequences:

>Translated_404_residues
MSILATPRSRLAAAALAVALAVGGGAFGVIRASASAPAAQAAPLQEVDVATVLSKTITDWQTYSGRLEAIERVDVRPLVS
GTIVSVNFKDGALVKKGDVLFVIDPRPYQAEVDRAAAQLAAAQARDGYAQTDWQRAQRLLGDNAIARRDYDEKQNAAREA
SANVKAAAAALEAARINLGYTRIVAPISGRASRAEITLGNVVSAGATAAPLTTLVSVSPIYASFDADEQTYLRYIGAVRD
GHRAPVELGLANESGYSRSGVIDSVDNRLDTSSGTIRVRARFDNADGTLVPGLYARLKVDGSAPHPALLVDDAAINTDQD
KKFVFVVDAQGKVAYREVRIGGLHGNQRVIAGGLQASDRVVVNGTQRVRPGEPVKVHLVPMTGGGGDAAVPEAAASQAAG
SAAS
>Mature_403_residues
SILATPRSRLAAAALAVALAVGGGAFGVIRASASAPAAQAAPLQEVDVATVLSKTITDWQTYSGRLEAIERVDVRPLVSG
TIVSVNFKDGALVKKGDVLFVIDPRPYQAEVDRAAAQLAAAQARDGYAQTDWQRAQRLLGDNAIARRDYDEKQNAAREAS
ANVKAAAAALEAARINLGYTRIVAPISGRASRAEITLGNVVSAGATAAPLTTLVSVSPIYASFDADEQTYLRYIGAVRDG
HRAPVELGLANESGYSRSGVIDSVDNRLDTSSGTIRVRARFDNADGTLVPGLYARLKVDGSAPHPALLVDDAAINTDQDK
KFVFVVDAQGKVAYREVRIGGLHGNQRVIAGGLQASDRVVVNGTQRVRPGEPVKVHLVPMTGGGGDAAVPEAAASQAAGS
AAS

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=363, Percent_Identity=34.435261707989, Blast_Score=198, Evalue=4e-52,
Organism=Escherichia coli, GI1789665, Length=318, Percent_Identity=35.8490566037736, Blast_Score=172, Evalue=2e-44,
Organism=Escherichia coli, GI1786668, Length=340, Percent_Identity=36.1764705882353, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI87082036, Length=356, Percent_Identity=30.6179775280899, Blast_Score=162, Evalue=4e-41,
Organism=Escherichia coli, GI87081951, Length=193, Percent_Identity=35.2331606217617, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI87081796, Length=316, Percent_Identity=26.5822784810127, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1789637, Length=243, Percent_Identity=30.8641975308642, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1790519, Length=247, Percent_Identity=28.3400809716599, Blast_Score=66, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42013; Mature: 41881

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.2 %Met     (Mature Protein)
0.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSILATPRSRLAAAALAVALAVGGGAFGVIRASASAPAAQAAPLQEVDVATVLSKTITDW
CCCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
QTYSGRLEAIERVDVRPLVSGTIVSVNFKDGALVKKGDVLFVIDPRPYQAEVDRAAAQLA
HHHCCHHHHHHHCCCCEECCCEEEEEEECCCCEEEECCEEEEECCCCCHHHHHHHHHHHH
AAQARDGYAQTDWQRAQRLLGDNAIARRDYDEKQNAAREASANVKAAAAALEAARINLGY
HHHHCCCCCCCHHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHEECCE
TRIVAPISGRASRAEITLGNVVSAGATAAPLTTLVSVSPIYASFDADEQTYLRYIGAVRD
EEEEEECCCCCCEEEEEECCEECCCCCHHHHHHHEECCCEEEECCCCHHHHHHHHHHHCC
GHRAPVELGLANESGYSRSGVIDSVDNRLDTSSGTIRVRARFDNADGTLVPGLYARLKVD
CCCCCEEEEECCCCCCCCCCCHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEEC
GSAPHPALLVDDAAINTDQDKKFVFVVDAQGKVAYREVRIGGLHGNQRVIAGGLQASDRV
CCCCCCEEEEECCCCCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCEEEEECCCCCCEE
VVNGTQRVRPGEPVKVHLVPMTGGGGDAAVPEAAASQAAGSAAS
EECCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHCCCCCCC
>Mature Secondary Structure 
SILATPRSRLAAAALAVALAVGGGAFGVIRASASAPAAQAAPLQEVDVATVLSKTITDW
CCCCCCHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHH
QTYSGRLEAIERVDVRPLVSGTIVSVNFKDGALVKKGDVLFVIDPRPYQAEVDRAAAQLA
HHHCCHHHHHHHCCCCEECCCEEEEEEECCCCEEEECCEEEEECCCCCHHHHHHHHHHHH
AAQARDGYAQTDWQRAQRLLGDNAIARRDYDEKQNAAREASANVKAAAAALEAARINLGY
HHHHCCCCCCCHHHHHHHHHCCCHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHEECCE
TRIVAPISGRASRAEITLGNVVSAGATAAPLTTLVSVSPIYASFDADEQTYLRYIGAVRD
EEEEEECCCCCCEEEEEECCEECCCCCHHHHHHHEECCCEEEECCCCHHHHHHHHHHHCC
GHRAPVELGLANESGYSRSGVIDSVDNRLDTSSGTIRVRARFDNADGTLVPGLYARLKVD
CCCCCEEEEECCCCCCCCCCCHHHHHCCCCCCCCEEEEEEEECCCCCCCCCCEEEEEEEC
GSAPHPALLVDDAAINTDQDKKFVFVVDAQGKVAYREVRIGGLHGNQRVIAGGLQASDRV
CCCCCCEEEEECCCCCCCCCCEEEEEEECCCCEEEEEEEEECCCCCCEEEEECCCCCCEE
VVNGTQRVRPGEPVKVHLVPMTGGGGDAAVPEAAASQAAGSAAS
EECCCCCCCCCCCEEEEEEEEECCCCCCCCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]