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The map label for this gene is aes [H]

Identifier: 238023815

GI number: 238023815

Start: 415824

End: 416675

Strand: Direct

Name: aes [H]

Synonym: bglu_2g03530

Alternate gene names: 238023815

Gene position: 415824-416675 (Clockwise)

Preceding gene: 238023812

Following gene: 238023816

Centisome position: 14.71

GC content: 71.6

Gene sequence:

>852_bases
ATGGACGCATCGGGTTTTCGCAATCTGCTGAACACCGCCACGGCCGCGCCGGGACAGGCGAGCACGCCGCTCGCGGTGTC
CGACGTCGAGATCGAGGGCCACGCGCAGGACATCGTGCTGCGCCTCTATCGCCGGCCGGACAAGACCGGATTGCCAGTAG
TGCTTTATTTCCACGGCGGCGGCTTCGTGCGCGGTTCGCTGGCCGACGCCGACTTCGCCGCGCGCTTCCTGGCCGAGCGC
ATGCCGGCGCTGGTGGTGTCGGTCGAGTATTCGCTGGCGCCGCAGCATCCGTTTCCGGCGGCCCCGGAGGATGCCTACCG
CGCCGCGCGCTGGGTGGAGGCGCGCGCCCGCGCGTTCGGCGGCAATCCGCGCCGCGTCGCGGTGGCCGGCCACGATGCGG
GCGGCCAGCTCGCTAACTGCCTCGCCTTCATCGCGCGCGACCGCGGCGACGTGACGATCGTCGCCCAGGCGCTGTTCGGA
CCGATGCTCGATCCGAGCATGACGCGGCTCGGCAACGAGCGCCGGCTCGCCTCCGACATCACCTTGAAGGAATGCGCGGC
CTGCTACCGCGCCTACCTGCCCCAGCCGGCGCAGCGCATGCATCCCTACGCGGCGCCCCTCGAATCGGCGCGGCTCGCGC
GGCTGCCCGCCACGCTGATCGCCACGGCCCACAACGACATGCTGCATGTCGAGGCTGAGAGGTACGCGGCCCGCCTGATC
GAGGCCGGCGTGCTGACCCAGGTGATCCGCTATCCCGACGTCTCGCATGCCGAGCTGGCCGGCTACGCGCCCGCGCTGCA
GGAGGCCGCGCGCTTCCTGAGCTGCCGGTTCCAGGCCCACGCCAACGACTGA

Upstream 100 bases:

>100_bases
GCCCCTGAATTGTGTTGCCGGATTATAGGCATTTATCCTGATGTCTGCCGGACCCAGAATGCGCTCCATCTTGCAATGGA
CGCGCTGACGCGCATCGCTC

Downstream 100 bases:

>100_bases
TTCCCGCCATTCCAACGCAACCGGAGATCTTCATGTCCATCCTAGCTACTCCCCGCTCTCGCCTCGCGGCGGCCGCGTTG
GCCGTCGCGCTCGCCGTCGG

Product: alpha/beta hydrolase fold-3 domain-containing protein

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MDASGFRNLLNTATAAPGQASTPLAVSDVEIEGHAQDIVLRLYRRPDKTGLPVVLYFHGGGFVRGSLADADFAARFLAER
MPALVVSVEYSLAPQHPFPAAPEDAYRAARWVEARARAFGGNPRRVAVAGHDAGGQLANCLAFIARDRGDVTIVAQALFG
PMLDPSMTRLGNERRLASDITLKECAACYRAYLPQPAQRMHPYAAPLESARLARLPATLIATAHNDMLHVEAERYAARLI
EAGVLTQVIRYPDVSHAELAGYAPALQEAARFLSCRFQAHAND

Sequences:

>Translated_283_residues
MDASGFRNLLNTATAAPGQASTPLAVSDVEIEGHAQDIVLRLYRRPDKTGLPVVLYFHGGGFVRGSLADADFAARFLAER
MPALVVSVEYSLAPQHPFPAAPEDAYRAARWVEARARAFGGNPRRVAVAGHDAGGQLANCLAFIARDRGDVTIVAQALFG
PMLDPSMTRLGNERRLASDITLKECAACYRAYLPQPAQRMHPYAAPLESARLARLPATLIATAHNDMLHVEAERYAARLI
EAGVLTQVIRYPDVSHAELAGYAPALQEAARFLSCRFQAHAND
>Mature_283_residues
MDASGFRNLLNTATAAPGQASTPLAVSDVEIEGHAQDIVLRLYRRPDKTGLPVVLYFHGGGFVRGSLADADFAARFLAER
MPALVVSVEYSLAPQHPFPAAPEDAYRAARWVEARARAFGGNPRRVAVAGHDAGGQLANCLAFIARDRGDVTIVAQALFG
PMLDPSMTRLGNERRLASDITLKECAACYRAYLPQPAQRMHPYAAPLESARLARLPATLIATAHNDMLHVEAERYAARLI
EAGVLTQVIRYPDVSHAELAGYAPALQEAARFLSCRFQAHAND

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=272, Percent_Identity=27.2058823529412, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI68299767, Length=280, Percent_Identity=27.5, Blast_Score=85, Evalue=9e-17,
Organism=Homo sapiens, GI21328446, Length=108, Percent_Identity=33.3333333333333, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1786682, Length=215, Percent_Identity=29.3023255813954, Blast_Score=72, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI72001146, Length=120, Percent_Identity=35.8333333333333, Blast_Score=72, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 30627; Mature: 30627

Theoretical pI: Translated: 7.64; Mature: 7.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDASGFRNLLNTATAAPGQASTPLAVSDVEIEGHAQDIVLRLYRRPDKTGLPVVLYFHGG
CCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEECC
GFVRGSLADADFAARFLAERMPALVVSVEYSLAPQHPFPAAPEDAYRAARWVEARARAFG
CEEECCCCCHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHC
GNPRRVAVAGHDAGGQLANCLAFIARDRGDVTIVAQALFGPMLDPSMTRLGNERRLASDI
CCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCHHHHHCCCCHHHHHCC
TLKECAACYRAYLPQPAQRMHPYAAPLESARLARLPATLIATAHNDMLHVEAERYAARLI
CHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHCCHHHHEECCCCEEEEHHHHHHHHHH
EAGVLTQVIRYPDVSHAELAGYAPALQEAARFLSCRFQAHAND
HHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDASGFRNLLNTATAAPGQASTPLAVSDVEIEGHAQDIVLRLYRRPDKTGLPVVLYFHGG
CCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHCCCCCCCCEEEEEECC
GFVRGSLADADFAARFLAERMPALVVSVEYSLAPQHPFPAAPEDAYRAARWVEARARAFG
CEEECCCCCHHHHHHHHHHHCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHC
GNPRRVAVAGHDAGGQLANCLAFIARDRGDVTIVAQALFGPMLDPSMTRLGNERRLASDI
CCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCHHHHHCCCCHHHHHCC
TLKECAACYRAYLPQPAQRMHPYAAPLESARLARLPATLIATAHNDMLHVEAERYAARLI
CHHHHHHHHHHHCCCCHHHCCCCCCCHHHHHHHHCCHHHHEECCCCEEEEHHHHHHHHHH
EAGVLTQVIRYPDVSHAELAGYAPALQEAARFLSCRFQAHAND
HHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA