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The map label for this gene is pcaF [H]

Identifier: 238023799

GI number: 238023799

Start: 395031

End: 396233

Strand: Direct

Name: pcaF [H]

Synonym: bglu_2g03350

Alternate gene names: 238023799

Gene position: 395031-396233 (Clockwise)

Preceding gene: 238023791

Following gene: 238023800

Centisome position: 13.97

GC content: 71.9

Gene sequence:

>1203_bases
ATGACCGATGCATTCCTCTGCGACGCGATCCGCACCCCGATCGGGCGATACGGCGGCGCGCTGTCGAGCGTCCGGGCCGA
CGATCTCGGCGCGGTGCCGATCAAGGCGCTCGTCGAGCGCAACCGCGAAGTCGACTGGGCCGCGCTCGACGACGTGATCT
ACGGCTGCGCGAACCAGGCCGGCGAGGACAACCGCAACGTGGCGCGCATGTCGGCGCTGCTGGCCGGCCTGCCGCTGGCG
GTGCCGGGCTCGACGGTCAATCGCCTGTGCGGGTCGGGCATGGACGCGATCGGCATCGCCGCGCGCGCCATCAAGTCCGG
CGAGGCCGGCCTGATGATCGCAGGGGGCGTGGAGAGCATGAGCCGCGCGCCGTTCGTGATGGGCAAGGCGAGCAGCGCGT
TCTCGCGTCAGGCCGAGATCTTCGACACGACGATCGGCTGGCGCTTCGTGAACCCGCTGATGAAGCAGCAATACGGCGTC
GATTCGATGCCCGAGACGGGCGAGAACGTCGCCACCGATTACGCGGTGAGCCGTGCCGACCAGGACGCGTTCGCGCTGCG
CAGCCAGCAGAAGGCGGCGCGCGCGCAGCAGGACGGCTCGCTCGCGCAGGAGATCGTCTCGGTCGCGATCGCGCAGAAGA
AGGGCGACCCGCTGGTGGTCGCGCGCGACGAGCATCCGCGCGAAACCTCGCTGGAAGCCCTGGCCAAGCTCAAGGGCGTG
GTGCGCCCGGACGGCACGGTGACGGCCGGCAACGCCTCGGGCGTCAACGACGGCGCCTGCGCGCTGCTGGTGGCCAACGA
GGAGAACGCGAAGCGCCACGGCCTGGTGCCGCGCGCGCGCATCCTCGGCATCGCCACCGCGGGCGTCGCGCCGCGCGTGA
TGGGCATCGGCCCGGCGCCGGCCACCCAGAAGCTGCTGGCGCGCCTGGGCATGACGCTCGACCAGTTCGACGTGATCGAG
CTGAACGAGGCGTTCGCCTCGCAGGGGCTCGCGGTGCTGCGCCTGCTGGGCGTGGCCGACGACGATCCGCGCGTGAACCC
GAACGGCGGCGCGATCGCGCTCGGCCATCCGCTCGGCGCCTCGGGCGCGCGGCTCGTGACCACCGCGATGTACCAGCTGC
AGCGCAGCTGCGGCCGCTTCGCGCTCTGCACGATGTGCATCGGCGTCGGCCAGGGCATCGCGCTCGCGATCGAGCGCGTC
TGA

Upstream 100 bases:

>100_bases
CGATCTTATGTTCGTATAAAGAACATTTTGACGCAACGCCGGCGCGCTTGCCGCGAGCCTTCTGGTGCGCGCCCCTTCCG
ACGATGTTCCGGAGACAACC

Downstream 100 bases:

>100_bases
GCAAGGGCGCGGCGCCGGCCTCGGGCCCGCGCCGCCGCCTGCCGCCCGCGTTTCCTTCCTTCCCTTCCTCCCGCTCCTGC
CGTTTCCCTTTCCCACCGGT

Product: beta-ketoadipyl CoA thiolase

Products: NA

Alternate protein names: 3-oxoadipyl-CoA thiolase [H]

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MTDAFLCDAIRTPIGRYGGALSSVRADDLGAVPIKALVERNREVDWAALDDVIYGCANQAGEDNRNVARMSALLAGLPLA
VPGSTVNRLCGSGMDAIGIAARAIKSGEAGLMIAGGVESMSRAPFVMGKASSAFSRQAEIFDTTIGWRFVNPLMKQQYGV
DSMPETGENVATDYAVSRADQDAFALRSQQKAARAQQDGSLAQEIVSVAIAQKKGDPLVVARDEHPRETSLEALAKLKGV
VRPDGTVTAGNASGVNDGACALLVANEENAKRHGLVPRARILGIATAGVAPRVMGIGPAPATQKLLARLGMTLDQFDVIE
LNEAFASQGLAVLRLLGVADDDPRVNPNGGAIALGHPLGASGARLVTTAMYQLQRSCGRFALCTMCIGVGQGIALAIERV

Sequences:

>Translated_400_residues
MTDAFLCDAIRTPIGRYGGALSSVRADDLGAVPIKALVERNREVDWAALDDVIYGCANQAGEDNRNVARMSALLAGLPLA
VPGSTVNRLCGSGMDAIGIAARAIKSGEAGLMIAGGVESMSRAPFVMGKASSAFSRQAEIFDTTIGWRFVNPLMKQQYGV
DSMPETGENVATDYAVSRADQDAFALRSQQKAARAQQDGSLAQEIVSVAIAQKKGDPLVVARDEHPRETSLEALAKLKGV
VRPDGTVTAGNASGVNDGACALLVANEENAKRHGLVPRARILGIATAGVAPRVMGIGPAPATQKLLARLGMTLDQFDVIE
LNEAFASQGLAVLRLLGVADDDPRVNPNGGAIALGHPLGASGARLVTTAMYQLQRSCGRFALCTMCIGVGQGIALAIERV
>Mature_399_residues
TDAFLCDAIRTPIGRYGGALSSVRADDLGAVPIKALVERNREVDWAALDDVIYGCANQAGEDNRNVARMSALLAGLPLAV
PGSTVNRLCGSGMDAIGIAARAIKSGEAGLMIAGGVESMSRAPFVMGKASSAFSRQAEIFDTTIGWRFVNPLMKQQYGVD
SMPETGENVATDYAVSRADQDAFALRSQQKAARAQQDGSLAQEIVSVAIAQKKGDPLVVARDEHPRETSLEALAKLKGVV
RPDGTVTAGNASGVNDGACALLVANEENAKRHGLVPRARILGIATAGVAPRVMGIGPAPATQKLLARLGMTLDQFDVIEL
NEAFASQGLAVLRLLGVADDDPRVNPNGGAIALGHPLGASGARLVTTAMYQLQRSCGRFALCTMCIGVGQGIALAIERV

Specific function: Catalyzes thiolytic cleavage of beta-ketoadipyl-CoA to succinyl-CoA and acetyl-CoA [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI167614485, Length=395, Percent_Identity=40.7594936708861, Blast_Score=268, Evalue=5e-72,
Organism=Homo sapiens, GI148539872, Length=397, Percent_Identity=37.7833753148615, Blast_Score=254, Evalue=1e-67,
Organism=Homo sapiens, GI4557237, Length=409, Percent_Identity=33.9853300733496, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI4501853, Length=406, Percent_Identity=38.6699507389163, Blast_Score=212, Evalue=6e-55,
Organism=Homo sapiens, GI4504327, Length=427, Percent_Identity=31.8501170960187, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI194353979, Length=398, Percent_Identity=28.894472361809, Blast_Score=116, Evalue=5e-26,
Organism=Escherichia coli, GI1787663, Length=401, Percent_Identity=67.8304239401496, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI1788554, Length=403, Percent_Identity=45.6575682382134, Blast_Score=287, Evalue=8e-79,
Organism=Escherichia coli, GI87082165, Length=401, Percent_Identity=44.6384039900249, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI48994986, Length=405, Percent_Identity=42.7160493827161, Blast_Score=280, Evalue=1e-76,
Organism=Escherichia coli, GI1788683, Length=410, Percent_Identity=32.4390243902439, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI133906874, Length=398, Percent_Identity=42.4623115577889, Blast_Score=291, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI25147385, Length=403, Percent_Identity=35.9801488833747, Blast_Score=234, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI17535921, Length=410, Percent_Identity=34.1463414634146, Blast_Score=202, Evalue=4e-52,
Organism=Caenorhabditis elegans, GI17551802, Length=430, Percent_Identity=33.2558139534884, Blast_Score=194, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI17535917, Length=402, Percent_Identity=31.0945273631841, Blast_Score=159, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6325229, Length=406, Percent_Identity=36.4532019704434, Blast_Score=219, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6322031, Length=409, Percent_Identity=33.7408312958435, Blast_Score=193, Evalue=5e-50,
Organism=Drosophila melanogaster, GI24655093, Length=401, Percent_Identity=43.142144638404, Blast_Score=310, Evalue=9e-85,
Organism=Drosophila melanogaster, GI17648125, Length=406, Percent_Identity=38.423645320197, Blast_Score=247, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24640423, Length=405, Percent_Identity=33.8271604938272, Blast_Score=206, Evalue=2e-53,
Organism=Drosophila melanogaster, GI17137578, Length=424, Percent_Identity=32.0754716981132, Blast_Score=178, Evalue=5e-45,
Organism=Drosophila melanogaster, GI24585051, Length=321, Percent_Identity=25.2336448598131, Blast_Score=75, Evalue=6e-14,
Organism=Drosophila melanogaster, GI19921506, Length=321, Percent_Identity=24.9221183800623, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012793
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.174 [H]

Molecular weight: Translated: 41661; Mature: 41530

Theoretical pI: Translated: 6.76; Mature: 6.76

Prosite motif: PS00098 THIOLASE_1 ; PS00737 THIOLASE_2 ; PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDAFLCDAIRTPIGRYGGALSSVRADDLGAVPIKALVERNREVDWAALDDVIYGCANQA
CCCHHHHHHHHCCHHHHCCHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCC
GEDNRNVARMSALLAGLPLAVPGSTVNRLCGSGMDAIGIAARAIKSGEAGLMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCHHHH
SRAPFVMGKASSAFSRQAEIFDTTIGWRFVNPLMKQQYGVDSMPETGENVATDYAVSRAD
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC
QDAFALRSQQKAARAQQDGSLAQEIVSVAIAQKKGDPLVVARDEHPRETSLEALAKLKGV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCC
VRPDGTVTAGNASGVNDGACALLVANEENAKRHGLVPRARILGIATAGVAPRVMGIGPAP
CCCCCCEECCCCCCCCCCEEEEEEECCCCCHHHCCCCHHHHEEEECCCCCCCEEECCCCC
ATQKLLARLGMTLDQFDVIELNEAFASQGLAVLRLLGVADDDPRVNPNGGAIALGHPLGA
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCC
SGARLVTTAMYQLQRSCGRFALCTMCIGVGQGIALAIERV
CCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEECC
>Mature Secondary Structure 
TDAFLCDAIRTPIGRYGGALSSVRADDLGAVPIKALVERNREVDWAALDDVIYGCANQA
CCHHHHHHHHCCHHHHCCHHHHCCCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHCC
GEDNRNVARMSALLAGLPLAVPGSTVNRLCGSGMDAIGIAARAIKSGEAGLMIAGGVESM
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEECCHHHH
SRAPFVMGKASSAFSRQAEIFDTTIGWRFVNPLMKQQYGVDSMPETGENVATDYAVSRAD
CCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCC
QDAFALRSQQKAARAQQDGSLAQEIVSVAIAQKKGDPLVVARDEHPRETSLEALAKLKGV
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHCC
VRPDGTVTAGNASGVNDGACALLVANEENAKRHGLVPRARILGIATAGVAPRVMGIGPAP
CCCCCCEECCCCCCCCCCEEEEEEECCCCCHHHCCCCHHHHEEEECCCCCCCEEECCCCC
ATQKLLARLGMTLDQFDVIELNEAFASQGLAVLRLLGVADDDPRVNPNGGAIALGHPLGA
HHHHHHHHHCCCCCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCCC
SGARLVTTAMYQLQRSCGRFALCTMCIGVGQGIALAIERV
CCHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]