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The map label for this gene is rspA [H]

Identifier: 238023670

GI number: 238023670

Start: 218915

End: 220123

Strand: Direct

Name: rspA [H]

Synonym: bglu_2g01910

Alternate gene names: 238023670

Gene position: 218915-220123 (Clockwise)

Preceding gene: 238023669

Following gene: 238023671

Centisome position: 7.74

GC content: 67.58

Gene sequence:

>1209_bases
GTGAAGATCGAACGGTTGCAGACCATCGTGACCTGTCCGGGCCGCAACTTCGTGACGGTGAAGATCGTCACGGACGAGGG
CGTGCACGGCCTCGGCGACGCCACGCTGAACGGGCGTGAACTCGCGGTGCGCGCGTACCTGGAGGAGCACGTGTTCCCGT
GTCTCGTCGGGCGCGATCCGCGCAACATCGAGGACATCTGGCAGTATCTCTATCGCGGCGCCTATTGGCGGCGCGGCCCG
GTGACGATGACGGCGATCGCGGCGATCGACATGGCGCTGTGGGACATCCTCGGCAAGCTGGCCGGCATGCCCGTCTACCG
TCTGCTCGGCGGCAAGAGCCGCGACGGCCTGATGGTGTACGGCCATGCCAACGGCCGCGACCACGAGGAAGCCGTGGACG
CGGTGCATCGGCATATCGAGGAGGGCTATCTGGCGATTCGCGTGCAGTCCGGCGTGCCGGGGCTCGACAAGGTCTACGGC
GTGGGCAAGGTGGCCGGCGAATACGAGCCGGCGCAAAAGGGCCTGCCGCCCGAGGAGCCCTGGGACACCGCGCTCTACTT
GCGCCACACGCCCGAGCTGTTCCGCAAGGTGCGCGAGGCGGTGGGCTTCGGGCCGCACCTGCTGCACGACGCGCACCACC
GCCTCACGCCGATCGAGGCGGGGCGGCTCGGCCGCGATCTCGAGCCGTACCGGCTGTTCTGGCTCGAAGACGCGACGCCT
GCCGAAAACCAGGAAAGCTTCCGGCTGATCCGCAGCCACACCACCACACCGCTGGCGGTGGGCGAGGTGTTCAATTCGAT
CTGGGACTGCAAGGACCTGATCCGCGAGCAATTGATCGACTACATCCGCTCGACCATCGTGCATGCGGGCGGCATCACGC
ATGCGCGGCGCATTGCCGACTACGCGGCGATGTACCAGGTGCGCACGGGTTTTCACGGCGCGACCGACCTGTCGCCCGTG
TGCATGGCGGCTGCCGTGAACTTCGGGCTATGGGCGCCGAACTTCGGCATCCAGGAGCTGATGCCGCACAACGCGCTGAC
CAACGAGGTGTTTCCGCACCGCTACCGCTTCGACCAGGGCTTCCTCGTGATGGACGACGCGCCGGGGCTCGGCGTGGAGA
TCGACGAGGCGCTCGCCGCGAAGCATCCCTACCAGCGCGCCTATCTGCCGGTCGCGCGGCTGCGCGACGGCGCAATGTGG
AACTGGTGA

Upstream 100 bases:

>100_bases
GCGCGGGCGGGTGCCCACGCATTTCACCGACTGACCCGGGCGCCACACGAACCGCCCGCCGGCCGCCGCGCCCCGGGCTG
CATGCAGAGGGAGGGAAACA

Downstream 100 bases:

>100_bases
GCGCCGCCGCGCGCGCCCCATCTCCAACGCCTTCAACACCGAGGAACCTGACATGCTGAGCGTCGTGGTCGATCGTCCGA
ACAGCCTGAGCGTGCGCGAA

Product: bifunctional D-altronate/D-mannonate dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MKIERLQTIVTCPGRNFVTVKIVTDEGVHGLGDATLNGRELAVRAYLEEHVFPCLVGRDPRNIEDIWQYLYRGAYWRRGP
VTMTAIAAIDMALWDILGKLAGMPVYRLLGGKSRDGLMVYGHANGRDHEEAVDAVHRHIEEGYLAIRVQSGVPGLDKVYG
VGKVAGEYEPAQKGLPPEEPWDTALYLRHTPELFRKVREAVGFGPHLLHDAHHRLTPIEAGRLGRDLEPYRLFWLEDATP
AENQESFRLIRSHTTTPLAVGEVFNSIWDCKDLIREQLIDYIRSTIVHAGGITHARRIADYAAMYQVRTGFHGATDLSPV
CMAAAVNFGLWAPNFGIQELMPHNALTNEVFPHRYRFDQGFLVMDDAPGLGVEIDEALAAKHPYQRAYLPVARLRDGAMW
NW

Sequences:

>Translated_402_residues
MKIERLQTIVTCPGRNFVTVKIVTDEGVHGLGDATLNGRELAVRAYLEEHVFPCLVGRDPRNIEDIWQYLYRGAYWRRGP
VTMTAIAAIDMALWDILGKLAGMPVYRLLGGKSRDGLMVYGHANGRDHEEAVDAVHRHIEEGYLAIRVQSGVPGLDKVYG
VGKVAGEYEPAQKGLPPEEPWDTALYLRHTPELFRKVREAVGFGPHLLHDAHHRLTPIEAGRLGRDLEPYRLFWLEDATP
AENQESFRLIRSHTTTPLAVGEVFNSIWDCKDLIREQLIDYIRSTIVHAGGITHARRIADYAAMYQVRTGFHGATDLSPV
CMAAAVNFGLWAPNFGIQELMPHNALTNEVFPHRYRFDQGFLVMDDAPGLGVEIDEALAAKHPYQRAYLPVARLRDGAMW
NW
>Mature_402_residues
MKIERLQTIVTCPGRNFVTVKIVTDEGVHGLGDATLNGRELAVRAYLEEHVFPCLVGRDPRNIEDIWQYLYRGAYWRRGP
VTMTAIAAIDMALWDILGKLAGMPVYRLLGGKSRDGLMVYGHANGRDHEEAVDAVHRHIEEGYLAIRVQSGVPGLDKVYG
VGKVAGEYEPAQKGLPPEEPWDTALYLRHTPELFRKVREAVGFGPHLLHDAHHRLTPIEAGRLGRDLEPYRLFWLEDATP
AENQESFRLIRSHTTTPLAVGEVFNSIWDCKDLIREQLIDYIRSTIVHAGGITHARRIADYAAMYQVRTGFHGATDLSPV
CMAAAVNFGLWAPNFGIQELMPHNALTNEVFPHRYRFDQGFLVMDDAPGLGVEIDEALAAKHPYQRAYLPVARLRDGAMW
NW

Specific function: Probably involved in the degradation of homoserine lactone (HSL) or of a metabolite of HSL that signals starvation [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787864, Length=404, Percent_Identity=64.3564356435644, Blast_Score=551, Evalue=1e-158,
Organism=Escherichia coli, GI48994953, Length=413, Percent_Identity=30.7506053268765, Blast_Score=165, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: NA

Molecular weight: Translated: 45277; Mature: 45277

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: PS00908 MR_MLE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIERLQTIVTCPGRNFVTVKIVTDEGVHGLGDATLNGRELAVRAYLEEHVFPCLVGRDP
CCHHHEEHEEECCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCC
RNIEDIWQYLYRGAYWRRGPVTMTAIAAIDMALWDILGKLAGMPVYRLLGGKSRDGLMVY
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEE
GHANGRDHEEAVDAVHRHIEEGYLAIRVQSGVPGLDKVYGVGKVAGEYEPAQKGLPPEEP
ECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHCCHHHCCCCCHHHCCCCCCCC
WDTALYLRHTPELFRKVREAVGFGPHLLHDAHHRLTPIEAGRLGRDLEPYRLFWLEDATP
CCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCC
AENQESFRLIRSHTTTPLAVGEVFNSIWDCKDLIREQLIDYIRSTIVHAGGITHARRIAD
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
YAAMYQVRTGFHGATDLSPVCMAAAVNFGLWAPNFGIQELMPHNALTNEVFPHRYRFDQG
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHCCCCCCEECCCC
FLVMDDAPGLGVEIDEALAAKHPYQRAYLPVARLRDGAMWNW
EEEEECCCCCCCCHHHHHHHCCCHHHHHCCHHHHCCCCCCCC
>Mature Secondary Structure
MKIERLQTIVTCPGRNFVTVKIVTDEGVHGLGDATLNGRELAVRAYLEEHVFPCLVGRDP
CCHHHEEHEEECCCCCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCC
RNIEDIWQYLYRGAYWRRGPVTMTAIAAIDMALWDILGKLAGMPVYRLLGGKSRDGLMVY
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEE
GHANGRDHEEAVDAVHRHIEEGYLAIRVQSGVPGLDKVYGVGKVAGEYEPAQKGLPPEEP
ECCCCCCHHHHHHHHHHHHHCCEEEEEECCCCCCHHHHHCCHHHCCCCCHHHCCCCCCCC
WDTALYLRHTPELFRKVREAVGFGPHLLHDAHHRLTPIEAGRLGRDLEPYRLFWLEDATP
CCCEEEEECCHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEEECCCC
AENQESFRLIRSHTTTPLAVGEVFNSIWDCKDLIREQLIDYIRSTIVHAGGITHARRIAD
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
YAAMYQVRTGFHGATDLSPVCMAAAVNFGLWAPNFGIQELMPHNALTNEVFPHRYRFDQG
HHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHCCCCHHHHCCCCCCEECCCC
FLVMDDAPGLGVEIDEALAAKHPYQRAYLPVARLRDGAMWNW
EEEEECCCCCCCCHHHHHHHCCCHHHHHCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7545940; 9097039; 9278503 [H]