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The map label for this gene is poxB [H]

Identifier: 238023626

GI number: 238023626

Start: 146704

End: 148428

Strand: Direct

Name: poxB [H]

Synonym: bglu_2g01330

Alternate gene names: 238023626

Gene position: 146704-148428 (Clockwise)

Preceding gene: 238023625

Following gene: 238023627

Centisome position: 5.19

GC content: 59.77

Gene sequence:

>1725_bases
ATGACGATGACCAATGCAGATCTGTTGACGCGCACACTTGCCAACGCAGGCGTTCAACGTATTTGGGGTGTGACCGGCGA
CAGCCTGAACGGTCTGAACGACAGCATGCGACGGCTCGGTCGCATTGCGTGGATGTCGGTGCGACACGAAGAAACGGCTG
CCTTTGCAGCCGGCGCGGAAGCGGCGATTACCGGCAAACTGGCCGTATGCGCCGGCAGTTGCGGACCAGGCAATCTTCAT
TTGATTAACGGCTTATTCGACTGCCATCGCAATCACGTTCCTGTGCTGGCCATCGCGGCTCATATTCCGTCGTCGGAGGT
CGGGCTCGGATATTTTCAGGAAACGCATCCACAAGAGCTGTTTCGCGAATGCAGTCACTTTGTGGAAGTCGTGTCCAATC
CGGCGCAGATGCCCGAGGTCCTTCATCGGGCGATGCGGACAGCCGTTGGGCGTCGCGGCGTTGCGGTTATCGTCATCTCG
GGTGATATCGCGCTTCAGAGCGCTCCGGCGGGCGCGTGTAGCGAATACGCGCCGGTCGTTGTGCCGAAGGCGCGCCCCAC
GGATGCGCAGCTCCAGGAACTGGCCGCCATGCTCAACCGCTCCCAGCGCGTGACGATTCTCGCCGGCAGTGGTTGTGCTG
GCACACACGACGCCTTGGTGACGCTCGCCGGCAAACTCGGTGCACCGATCGTCCATGCATTGCGTGGCAAGGAGCATGTG
GAATGGGACAATCCATATGACGTCGGCATGACCGGCCTGATCGGCTTTTCTTCCGGCTATCAGGCCATGCTGGGCTGCAG
CACGCTGTTGATGCTAGGCACCGACTTTCCTTACCGCCACTTTTATCCGAAGTCGGCCAAGATCATTCAAGTCGATTGCG
ACCCGTCTGCACTCGGACGACGCGCCCAGCTTTCTCTCGCGATCGCGGCTGATGTCGGAGAGACAATCGCGGCCTTGCTT
CCCCTGCTGCATGGTGAGCGCGATCCAGGCTTCCTCCACGCAGCGCGCTCGCACTACGCGAAAGCCCGCGCCGAGTTGGA
CGAGCTCGCGCAGCCCAGTGCGTCGGGCCGGCCTATACACCCGCAATATCTCGCACGCCTTATCAGCGACCAAGCGGCCG
ACGATGCGATTTTTACCGCAGATGTCGGCACTCCCACGGTTTGGGCGGCGCGCTATTTGGCCATGAACGGCCGCCGCCGT
TTGCTCGGTTCGTTTAATCACGGATCAATGGCCAATGCGATGTTGCAAGCAATCGGTGCGCAGGTAGCGGCCCCGGGCCG
AGAGGTCGTCTCGTTGTCCGGCGACGGCGGTTTCTCGATGATGATGGGAGACCTGCTTACGATCAAGCAACTCGACCTGC
CGGTGAAGATCATCGTCTTCAACAACGGGGTGCTCGGGTTCGTGGCCATGGAGATGAAGGCCGCCGGCTATCTCGACACC
GGTGTGGACCTTCTCAATCCGGACTTTGCCGCGCTAGCCAATGTTGTCGGCATCAAAGGTATCCGGGTCGAGAATTCGAT
CGAATTGCCCGAAGCGATCAAGCAAACGTTCACTCACCGTGGTCCCGTCCTTCTTGATGTCGTGACGGCGAAGGAGGAAC
TGGTGATACCGCCGAAAGTCCAGGCCGAACAGGCTAAAGGGTTCAGTCTTTATATGCTTAAAGCGATCCTCAACGGGCGC
GGCGATGAAGTCCTCGATATCGCGCACACGAATATCAGAGACTGA

Upstream 100 bases:

>100_bases
AAATGCGGGTTGCAATGACCCTGACCGGCGCGCGCTCGATTGCCGACATAAGCCGCTCTAACCTGGTGTCTCCCGGACTC
GAAACCATGGAGCTTGCAGC

Downstream 100 bases:

>100_bases
GGATGCATGCCGGGTGAGCCGGGAAAGGCGTGAGCTGCCCCGAGCCTCGAGGAGGCAGGTTGGTTTAGCGGCCATTTCAA
AAACTTCAAGAGGGGCTGCT

Product: Pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MTMTNADLLTRTLANAGVQRIWGVTGDSLNGLNDSMRRLGRIAWMSVRHEETAAFAAGAEAAITGKLAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEVGLGYFQETHPQELFRECSHFVEVVSNPAQMPEVLHRAMRTAVGRRGVAVIVIS
GDIALQSAPAGACSEYAPVVVPKARPTDAQLQELAAMLNRSQRVTILAGSGCAGTHDALVTLAGKLGAPIVHALRGKEHV
EWDNPYDVGMTGLIGFSSGYQAMLGCSTLLMLGTDFPYRHFYPKSAKIIQVDCDPSALGRRAQLSLAIAADVGETIAALL
PLLHGERDPGFLHAARSHYAKARAELDELAQPSASGRPIHPQYLARLISDQAADDAIFTADVGTPTVWAARYLAMNGRRR
LLGSFNHGSMANAMLQAIGAQVAAPGREVVSLSGDGGFSMMMGDLLTIKQLDLPVKIIVFNNGVLGFVAMEMKAAGYLDT
GVDLLNPDFAALANVVGIKGIRVENSIELPEAIKQTFTHRGPVLLDVVTAKEELVIPPKVQAEQAKGFSLYMLKAILNGR
GDEVLDIAHTNIRD

Sequences:

>Translated_574_residues
MTMTNADLLTRTLANAGVQRIWGVTGDSLNGLNDSMRRLGRIAWMSVRHEETAAFAAGAEAAITGKLAVCAGSCGPGNLH
LINGLFDCHRNHVPVLAIAAHIPSSEVGLGYFQETHPQELFRECSHFVEVVSNPAQMPEVLHRAMRTAVGRRGVAVIVIS
GDIALQSAPAGACSEYAPVVVPKARPTDAQLQELAAMLNRSQRVTILAGSGCAGTHDALVTLAGKLGAPIVHALRGKEHV
EWDNPYDVGMTGLIGFSSGYQAMLGCSTLLMLGTDFPYRHFYPKSAKIIQVDCDPSALGRRAQLSLAIAADVGETIAALL
PLLHGERDPGFLHAARSHYAKARAELDELAQPSASGRPIHPQYLARLISDQAADDAIFTADVGTPTVWAARYLAMNGRRR
LLGSFNHGSMANAMLQAIGAQVAAPGREVVSLSGDGGFSMMMGDLLTIKQLDLPVKIIVFNNGVLGFVAMEMKAAGYLDT
GVDLLNPDFAALANVVGIKGIRVENSIELPEAIKQTFTHRGPVLLDVVTAKEELVIPPKVQAEQAKGFSLYMLKAILNGR
GDEVLDIAHTNIRD
>Mature_573_residues
TMTNADLLTRTLANAGVQRIWGVTGDSLNGLNDSMRRLGRIAWMSVRHEETAAFAAGAEAAITGKLAVCAGSCGPGNLHL
INGLFDCHRNHVPVLAIAAHIPSSEVGLGYFQETHPQELFRECSHFVEVVSNPAQMPEVLHRAMRTAVGRRGVAVIVISG
DIALQSAPAGACSEYAPVVVPKARPTDAQLQELAAMLNRSQRVTILAGSGCAGTHDALVTLAGKLGAPIVHALRGKEHVE
WDNPYDVGMTGLIGFSSGYQAMLGCSTLLMLGTDFPYRHFYPKSAKIIQVDCDPSALGRRAQLSLAIAADVGETIAALLP
LLHGERDPGFLHAARSHYAKARAELDELAQPSASGRPIHPQYLARLISDQAADDAIFTADVGTPTVWAARYLAMNGRRRL
LGSFNHGSMANAMLQAIGAQVAAPGREVVSLSGDGGFSMMMGDLLTIKQLDLPVKIIVFNNGVLGFVAMEMKAAGYLDTG
VDLLNPDFAALANVVGIKGIRVENSIELPEAIKQTFTHRGPVLLDVVTAKEELVIPPKVQAEQAKGFSLYMLKAILNGRG
DEVLDIAHTNIRD

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=560, Percent_Identity=22.8571428571429, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1787096, Length=571, Percent_Identity=63.922942206655, Blast_Score=744, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=553, Percent_Identity=28.0289330922242, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI87081685, Length=560, Percent_Identity=25.5357142857143, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI1786717, Length=558, Percent_Identity=24.3727598566308, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=488, Percent_Identity=24.7950819672131, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=487, Percent_Identity=24.8459958932238, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=577, Percent_Identity=23.5701906412478, Blast_Score=163, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6320816, Length=494, Percent_Identity=21.6599190283401, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI19922626, Length=482, Percent_Identity=26.1410788381743, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 61128; Mature: 60997

Theoretical pI: Translated: 6.82; Mature: 6.82

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMTNADLLTRTLANAGVQRIWGVTGDSLNGLNDSMRRLGRIAWMSVRHEETAAFAAGAE
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEECCHHHHHHHCCCC
AAITGKLAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEVGLGYFQETHPQEL
CEEECEEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHCCCHHHH
FRECSHFVEVVSNPAQMPEVLHRAMRTAVGRRGVAVIVISGDIALQSAPAGACSEYAPVV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEECCCCCCCCCCCCEE
VPKARPTDAQLQELAAMLNRSQRVTILAGSGCAGTHDALVTLAGKLGAPIVHALRGKEHV
ECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCC
EWDNPYDVGMTGLIGFSSGYQAMLGCSTLLMLGTDFPYRHFYPKSAKIIQVDCDPSALGR
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHCC
RAQLSLAIAADVGETIAALLPLLHGERDPGFLHAARSHYAKARAELDELAQPSASGRPIH
CEEEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PQYLARLISDQAADDAIFTADVGTPTVWAARYLAMNGRRRLLGSFNHGSMANAMLQAIGA
HHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHH
QVAAPGREVVSLSGDGGFSMMMGDLLTIKQLDLPVKIIVFNNGVLGFVAMEMKAAGYLDT
HHCCCCCEEEEECCCCCHHHHHHHHHHHEECCCCEEEEEECCCCEEEEEEHHHHCCCCCC
GVDLLNPDFAALANVVGIKGIRVENSIELPEAIKQTFTHRGPVLLDVVTAKEELVIPPKV
CCHHCCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCEECCCCC
QAEQAKGFSLYMLKAILNGRGDEVLDIAHTNIRD
CHHHCCCCHHHHHHHHHCCCCCCEEEECCCCCCC
>Mature Secondary Structure 
TMTNADLLTRTLANAGVQRIWGVTGDSLNGLNDSMRRLGRIAWMSVRHEETAAFAAGAE
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHEECCHHHHHHHCCCC
AAITGKLAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHIPSSEVGLGYFQETHPQEL
CEEECEEEEEECCCCCCCEEEEHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHCCCHHHH
FRECSHFVEVVSNPAQMPEVLHRAMRTAVGRRGVAVIVISGDIALQSAPAGACSEYAPVV
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEEECCCCCCCCCCCCEE
VPKARPTDAQLQELAAMLNRSQRVTILAGSGCAGTHDALVTLAGKLGAPIVHALRGKEHV
ECCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCC
EWDNPYDVGMTGLIGFSSGYQAMLGCSTLLMLGTDFPYRHFYPKSAKIIQVDCDPSALGR
CCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEECCHHHHCC
RAQLSLAIAADVGETIAALLPLLHGERDPGFLHAARSHYAKARAELDELAQPSASGRPIH
CEEEEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PQYLARLISDQAADDAIFTADVGTPTVWAARYLAMNGRRRLLGSFNHGSMANAMLQAIGA
HHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHH
QVAAPGREVVSLSGDGGFSMMMGDLLTIKQLDLPVKIIVFNNGVLGFVAMEMKAAGYLDT
HHCCCCCEEEEECCCCCHHHHHHHHHHHEECCCCEEEEEECCCCEEEEEEHHHHCCCCCC
GVDLLNPDFAALANVVGIKGIRVENSIELPEAIKQTFTHRGPVLLDVVTAKEELVIPPKV
CCHHCCCCHHHHHHHHCCCCEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCEECCCCC
QAEQAKGFSLYMLKAILNGRGDEVLDIAHTNIRD
CHHHCCCCHHHHHHHHHCCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]