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The map label for this gene is lcfB [H]

Identifier: 238023615

GI number: 238023615

Start: 131992

End: 133467

Strand: Direct

Name: lcfB [H]

Synonym: bglu_2g01200

Alternate gene names: 238023615

Gene position: 131992-133467 (Clockwise)

Preceding gene: 238023614

Following gene: 238023616

Centisome position: 4.67

GC content: 64.77

Gene sequence:

>1476_bases
ATGATCAGCGCACTCATCGAACGCCATTTCGCCGAAGCGCCGAACCGTATTGTCGTGCGGGAGATGGACGGCCGTGAATA
CTCGCTCGATGAACTGCGCGCAGACGTCGCGCGGATCGGTGGCAAGCTGGAAGAGATATACGGCGACTGCAGCGGTCTCG
TGTTCGGTATTGCCGCGCGCTCCAGCTACACGTGGGTGGCAACGATGCTCGCGATCTTGCGCGTGAAGGCGGTGCTCCTG
CCGGTGCCGATCGAGTTTTCCGACGAACAGATCGGCAGTCTGCTGCACAAGGCAACCGCGATTTTCACGCAGGATGCGAA
AACGGCCGCCCGCATCGGCTCGATCCTGCCGGGCATCTCGTGCATCAACGCGGCGGGCGACCATGACGAATGGCCCGCAG
CAGGCGCGCCCGCCGGCGAGCGGATCGAGCCGGGCATCTGCGGGATCATCCATACGTCCGGTACGACGTCGAAGCCGAAG
GGGGTGAAGATCCGCGACGAGGCTGTCGGCCTGCTGGTGACCAACGTCCTGAAGCGGGTGCCGGCCGGCCCGCTCGACTA
CCTGTCGATCGTGCCGATGAGCCTGCTGATCGAACAGGTGCTCGGCGTATTCCTGCCGGTGCTGTCCGGCGGTTCGCTGA
CGCTGATGCCCGCGAACTACGCGGAGTACGGCGCGCGCAGCGGCAATGCGCGCGAATACCTGGACCTGATCGCCCGTGTC
GATCCGAACTTCCTGTATCTGCCGCCAAAGCTGCTCGAGGAGGCCGATGCGCTGCTGGAGTCGATCGCCGCGACGCAACT
GTTCGGCCGCCGCAACCCGCACATCATCACGGGCGGCGCGAAGATTCCGGCCACCGTGCTCGAATCGCTGGACAAGCGCG
GCGTGCAGGTCTACGAAGCGTACGGTCTCAGCGAGAACAGTTCGATCATCTCGCTGAACTATCCGGGGCAACGCCGCATC
GGCTCCGCCGGAACCTTCCTCGACGGGATCGAGCCGGTGATCGTCGACGGCGAGCTGCGCGTGCGCACGCCGACGCTCTG
CGCGGGCTACTACAACTCGGACGATACGTCGTGCGAACTCACCGACGGCTACCTGCATACCGGCGACCTCGCCGAGATCG
TCGATGGTTTCCTGTACATCACCGGCCGCAAGAAGCACGTGATCATCCTGTCCACGGCGCGCAACATCTCGCCGGAGTGG
GTGGAGACGGTTTACAAGGAAAGCGCGCTGATCGACGACATCGTGGTGTTCGGCGAAGGGCGCGAAGAGCCGGCGGCGAT
CGTACTGTCCACGCATGACGAAGCGGCCGTGCGCAACGAGATGGCACGGATCGAGCCGCGGCTCGCGGATTTCGCACGCG
TGCGGCGGTTCCGGGTGGTGACGGACATCGAGCGTTTCCGCAAGGATTACTACACGGTCACCGGGCGACCGCGCCGTCAG
CTGATCGAGCGCGATCACGCGGCGTCGCTTTACTGA

Upstream 100 bases:

>100_bases
TCGTGATCGACAACAGCCGCGTGATGCACGGCCGCCGCCGGCTCGCGGACACGGGACGCCGCATCTTCGGTGCGCAAAGC
TATCGCAAGGGAGCCATCGC

Downstream 100 bases:

>100_bases
TCGGGAGCTGATCGTGCCCATCGTACGAACCTTTCATCCCGGCCAGGACGCCTATCGCGAGATTCAGCTCGCGGTCTCGC
AGCACTACTACCAGGCGCAC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MISALIERHFAEAPNRIVVREMDGREYSLDELRADVARIGGKLEEIYGDCSGLVFGIAARSSYTWVATMLAILRVKAVLL
PVPIEFSDEQIGSLLHKATAIFTQDAKTAARIGSILPGISCINAAGDHDEWPAAGAPAGERIEPGICGIIHTSGTTSKPK
GVKIRDEAVGLLVTNVLKRVPAGPLDYLSIVPMSLLIEQVLGVFLPVLSGGSLTLMPANYAEYGARSGNAREYLDLIARV
DPNFLYLPPKLLEEADALLESIAATQLFGRRNPHIITGGAKIPATVLESLDKRGVQVYEAYGLSENSSIISLNYPGQRRI
GSAGTFLDGIEPVIVDGELRVRTPTLCAGYYNSDDTSCELTDGYLHTGDLAEIVDGFLYITGRKKHVIILSTARNISPEW
VETVYKESALIDDIVVFGEGREEPAAIVLSTHDEAAVRNEMARIEPRLADFARVRRFRVVTDIERFRKDYYTVTGRPRRQ
LIERDHAASLY

Sequences:

>Translated_491_residues
MISALIERHFAEAPNRIVVREMDGREYSLDELRADVARIGGKLEEIYGDCSGLVFGIAARSSYTWVATMLAILRVKAVLL
PVPIEFSDEQIGSLLHKATAIFTQDAKTAARIGSILPGISCINAAGDHDEWPAAGAPAGERIEPGICGIIHTSGTTSKPK
GVKIRDEAVGLLVTNVLKRVPAGPLDYLSIVPMSLLIEQVLGVFLPVLSGGSLTLMPANYAEYGARSGNAREYLDLIARV
DPNFLYLPPKLLEEADALLESIAATQLFGRRNPHIITGGAKIPATVLESLDKRGVQVYEAYGLSENSSIISLNYPGQRRI
GSAGTFLDGIEPVIVDGELRVRTPTLCAGYYNSDDTSCELTDGYLHTGDLAEIVDGFLYITGRKKHVIILSTARNISPEW
VETVYKESALIDDIVVFGEGREEPAAIVLSTHDEAAVRNEMARIEPRLADFARVRRFRVVTDIERFRKDYYTVTGRPRRQ
LIERDHAASLY
>Mature_491_residues
MISALIERHFAEAPNRIVVREMDGREYSLDELRADVARIGGKLEEIYGDCSGLVFGIAARSSYTWVATMLAILRVKAVLL
PVPIEFSDEQIGSLLHKATAIFTQDAKTAARIGSILPGISCINAAGDHDEWPAAGAPAGERIEPGICGIIHTSGTTSKPK
GVKIRDEAVGLLVTNVLKRVPAGPLDYLSIVPMSLLIEQVLGVFLPVLSGGSLTLMPANYAEYGARSGNAREYLDLIARV
DPNFLYLPPKLLEEADALLESIAATQLFGRRNPHIITGGAKIPATVLESLDKRGVQVYEAYGLSENSSIISLNYPGQRRI
GSAGTFLDGIEPVIVDGELRVRTPTLCAGYYNSDDTSCELTDGYLHTGDLAEIVDGFLYITGRKKHVIILSTARNISPEW
VETVYKESALIDDIVVFGEGREEPAAIVLSTHDEAAVRNEMARIEPRLADFARVRRFRVVTDIERFRKDYYTVTGRPRRQ
LIERDHAASLY

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG1022

COG function: function code I; Long-chain acyl-CoA synthetases (AMP-forming)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI40807491, Length=330, Percent_Identity=26.3636363636364, Blast_Score=84, Evalue=4e-16,
Organism=Homo sapiens, GI27477105, Length=337, Percent_Identity=24.0356083086053, Blast_Score=83, Evalue=7e-16,
Organism=Homo sapiens, GI57165410, Length=248, Percent_Identity=28.2258064516129, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI57165412, Length=248, Percent_Identity=28.2258064516129, Blast_Score=77, Evalue=4e-14,
Organism=Escherichia coli, GI145693145, Length=362, Percent_Identity=23.4806629834254, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1788107, Length=189, Percent_Identity=31.7460317460317, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17556552, Length=427, Percent_Identity=23.6533957845433, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17558820, Length=279, Percent_Identity=30.1075268817204, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17510401, Length=168, Percent_Identity=30.3571428571429, Blast_Score=69, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17541856, Length=183, Percent_Identity=28.4153005464481, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 53722; Mature: 53722

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISALIERHFAEAPNRIVVREMDGREYSLDELRADVARIGGKLEEIYGDCSGLVFGIAAR
CHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCEEEEECC
SSYTWVATMLAILRVKAVLLPVPIEFSDEQIGSLLHKATAIFTQDAKTAARIGSILPGIS
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCH
CINAAGDHDEWPAAGAPAGERIEPGICGIIHTSGTTSKPKGVKIRDEAVGLLVTNVLKRV
HHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHC
PAGPLDYLSIVPMSLLIEQVLGVFLPVLSGGSLTLMPANYAEYGARSGNAREYLDLIARV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCHHHHHHHHHHC
DPNFLYLPPKLLEEADALLESIAATQLFGRRNPHIITGGAKIPATVLESLDKRGVQVYEA
CCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCEEEEE
YGLSENSSIISLNYPGQRRIGSAGTFLDGIEPVIVDGELRVRTPTLCAGYYNSDDTSCEL
ECCCCCCCEEEECCCCCCCCCCCCCHHCCCCEEEECCEEEEECCEEEEECCCCCCCCEEE
TDGYLHTGDLAEIVDGFLYITGRKKHVIILSTARNISPEWVETVYKESALIDDIVVFGEG
ECCEEECCCHHHHHCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEECCC
REEPAAIVLSTHDEAAVRNEMARIEPRLADFARVRRFRVVTDIERFRKDYYTVTGRPRRQ
CCCCCEEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHH
LIERDHAASLY
HHHHHHHCCCC
>Mature Secondary Structure
MISALIERHFAEAPNRIVVREMDGREYSLDELRADVARIGGKLEEIYGDCSGLVFGIAAR
CHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHCCHHHHHHCCCCCCEEEEECC
SSYTWVATMLAILRVKAVLLPVPIEFSDEQIGSLLHKATAIFTQDAKTAARIGSILPGIS
CCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCH
CINAAGDHDEWPAAGAPAGERIEPGICGIIHTSGTTSKPKGVKIRDEAVGLLVTNVLKRV
HHCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCEEHHHHHHHHHHHHHHHC
PAGPLDYLSIVPMSLLIEQVLGVFLPVLSGGSLTLMPANYAEYGARSGNAREYLDLIARV
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHCCCCCCHHHHHHHHHHC
DPNFLYLPPKLLEEADALLESIAATQLFGRRNPHIITGGAKIPATVLESLDKRGVQVYEA
CCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHCCCEEEEE
YGLSENSSIISLNYPGQRRIGSAGTFLDGIEPVIVDGELRVRTPTLCAGYYNSDDTSCEL
ECCCCCCCEEEECCCCCCCCCCCCCHHCCCCEEEECCEEEEECCEEEEECCCCCCCCEEE
TDGYLHTGDLAEIVDGFLYITGRKKHVIILSTARNISPEWVETVYKESALIDDIVVFGEG
ECCEEECCCHHHHHCCEEEEECCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEECCC
REEPAAIVLSTHDEAAVRNEMARIEPRLADFARVRRFRVVTDIERFRKDYYTVTGRPRRQ
CCCCCEEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCHHHH
LIERDHAASLY
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]