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The map label for this gene is pcaB [H]

Identifier: 238023562

GI number: 238023562

Start: 66456

End: 67814

Strand: Direct

Name: pcaB [H]

Synonym: bglu_2g00640

Alternate gene names: 238023562

Gene position: 66456-67814 (Clockwise)

Preceding gene: 238023561

Following gene: 238023563

Centisome position: 2.35

GC content: 75.35

Gene sequence:

>1359_bases
ATGTTTGAACACAGCGCCCGCTTGACTTCCCTGATCGGCGGCGATGCCGCGCTGATCCGGATCTGGTCGCCGACGGCGAC
GCTGCAGCGCATGCTCGACGTCGAGGCCGCGCTCGCGCGTGCGCTCGCCGCGCACGGCGTGATTCCGGCCGCCGCGGTCG
CGCCGATCGAGGCCGCCTGCCGCGCCGAACGGCTCGACGCCGAGGCACTCGCGCGCGATGCCGCGCTCGGCGGCAACCTC
GCGATTCCGCTCGTCAAGCAGCTGACCGCGCGCGTCAAGGACGCCGACGCCGAGGCGGCGAAGTTCGTCCACTGGGGCGC
GACGAGCCAGGACATCCTCGACACCGGCCTCGTGCTGCAGCTGCGCGAGACCTTCGACGCGCTCGCGCTGCGGCTCGACA
CGGTCTGCGCGTCGCTTGCCGAGCTGGCGCGCGTGCACCGCGCCACGCCGATGATCGGCCGCACCTGGCTGCAGCAGGCG
CTGCCGATCACGCTGGGCCTGAAGTTCGCGCAATGGCTCGACGCGCTGGGCCGCCACCGCCGGCGTCTCGCCGCGCTGCG
CGGCGAGGTGCTGGTGCTGCAGTTCGGCGGCGCGGCCGGCACGCTCGCGAGCCTGCGCGAGGCCGCGCCGCGCGTGGCCG
CCTCGCTCGCGGCCGAGCTGGCGCTCGCGCTGCCCGACCTGCCGTGGCACACGCAGCGCGACCGCATCGCCGAGACGGCC
GCGTTCTTCGCGATGGTGATCGGCACGCTCGGCAAGATCGCGCGCGACATCTCCCTGCAGATGCAGACCGAGATCGGCGA
GCTGGCCGAGCCCGCCGCGGCCGGCAAGGGCGGCTCGTCCACCATGCCGCACAAGCGCAACCCGGTCGGCTGCGCGGCCG
TGCTGAGCGCCGCCGTGCGCGCGCCGGGGCTTGCCGCCACCGTGTTCGCCGGGATGGTGCAGGAGCACGAGCGCGCGCTG
GGCGGCTGGCAGGCCGAGTGGGACGCGCTGCCCGAACTCGCGCGGCTCGCGGGCGGCGCGCTCGCCGGTGTCGCGCAGAT
CGTCGGCGGCCTGCAGGTCGACACCGCGCGGCTTGCCGCCAACCTCGACGCCACGCGCGGCCTGGTGCTCGGCGAGGCCG
TGATGCTCGCGCTCGGCGACCGGATCGGCCGGCTCGACGCGCACCACCTGGTCGAGCAGGCCTCGCAGGAAGCGGTGCGC
ACCGGCCGCACGCTGCACGAGGTGCTGGCCGCCGAGCCGGCCGTCGGCGCGCACCTGACGCCCGACGCGCTGCGCCGGCT
GCTCGATCCCGCCCATTACGTCGGCGAAGCCGCTGCGTTCGTCGATGCCGTGCTCGTGCGCCACGCGGGCCACGCCTAA

Upstream 100 bases:

>100_bases
GCTGCCTGCCGCCGGCACCACGCCGGCGCGGCCGGCGCACCCTGCCGCGCTTCGGTGGACAATACGCGCTATCGGCCCTT
TTCGACCTTGCCATCCTCCC

Downstream 100 bases:

>100_bases
ACCAAGGAGAACCAACGATGCCCTTTGCTTCTGTCAACGGTGTGAAGCTGCATTACCGCATCGACCGCGCCGCGAGCGCC
GACGCACCGTGGCTGGTATT

Product: 3-carboxy-cis,cis-muconate cycloisomerase

Products: NA

Alternate protein names: 3-carboxymuconate lactonizing enzyme; CMLE [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MFEHSARLTSLIGGDAALIRIWSPTATLQRMLDVEAALARALAAHGVIPAAAVAPIEAACRAERLDAEALARDAALGGNL
AIPLVKQLTARVKDADAEAAKFVHWGATSQDILDTGLVLQLRETFDALALRLDTVCASLAELARVHRATPMIGRTWLQQA
LPITLGLKFAQWLDALGRHRRRLAALRGEVLVLQFGGAAGTLASLREAAPRVAASLAAELALALPDLPWHTQRDRIAETA
AFFAMVIGTLGKIARDISLQMQTEIGELAEPAAAGKGGSSTMPHKRNPVGCAAVLSAAVRAPGLAATVFAGMVQEHERAL
GGWQAEWDALPELARLAGGALAGVAQIVGGLQVDTARLAANLDATRGLVLGEAVMLALGDRIGRLDAHHLVEQASQEAVR
TGRTLHEVLAAEPAVGAHLTPDALRRLLDPAHYVGEAAAFVDAVLVRHAGHA

Sequences:

>Translated_452_residues
MFEHSARLTSLIGGDAALIRIWSPTATLQRMLDVEAALARALAAHGVIPAAAVAPIEAACRAERLDAEALARDAALGGNL
AIPLVKQLTARVKDADAEAAKFVHWGATSQDILDTGLVLQLRETFDALALRLDTVCASLAELARVHRATPMIGRTWLQQA
LPITLGLKFAQWLDALGRHRRRLAALRGEVLVLQFGGAAGTLASLREAAPRVAASLAAELALALPDLPWHTQRDRIAETA
AFFAMVIGTLGKIARDISLQMQTEIGELAEPAAAGKGGSSTMPHKRNPVGCAAVLSAAVRAPGLAATVFAGMVQEHERAL
GGWQAEWDALPELARLAGGALAGVAQIVGGLQVDTARLAANLDATRGLVLGEAVMLALGDRIGRLDAHHLVEQASQEAVR
TGRTLHEVLAAEPAVGAHLTPDALRRLLDPAHYVGEAAAFVDAVLVRHAGHA
>Mature_452_residues
MFEHSARLTSLIGGDAALIRIWSPTATLQRMLDVEAALARALAAHGVIPAAAVAPIEAACRAERLDAEALARDAALGGNL
AIPLVKQLTARVKDADAEAAKFVHWGATSQDILDTGLVLQLRETFDALALRLDTVCASLAELARVHRATPMIGRTWLQQA
LPITLGLKFAQWLDALGRHRRRLAALRGEVLVLQFGGAAGTLASLREAAPRVAASLAAELALALPDLPWHTQRDRIAETA
AFFAMVIGTLGKIARDISLQMQTEIGELAEPAAAGKGGSSTMPHKRNPVGCAAVLSAAVRAPGLAATVFAGMVQEHERAL
GGWQAEWDALPELARLAGGALAGVAQIVGGLQVDTARLAANLDATRGLVLGEAVMLALGDRIGRLDAHHLVEQASQEAVR
TGRTLHEVLAAEPAVGAHLTPDALRRLLDPAHYVGEAAAFVDAVLVRHAGHA

Specific function: Catalyzes an anti cycloisomerization [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II fumarase/aspartase family [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=365, Percent_Identity=25.4794520547945, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1787376, Length=291, Percent_Identity=27.4914089347079, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI17508577, Length=315, Percent_Identity=26.031746031746, Blast_Score=75, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323391, Length=371, Percent_Identity=24.5283018867925, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR012789 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =5.5.1.2 [H]

Molecular weight: Translated: 47349; Mature: 47349

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFEHSARLTSLIGGDAALIRIWSPTATLQRMLDVEAALARALAAHGVIPAAAVAPIEAAC
CCCCHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RAERLDAEALARDAALGGNLAIPLVKQLTARVKDADAEAAKFVHWGATSQDILDTGLVLQ
HHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHH
LRETFDALALRLDTVCASLAELARVHRATPMIGRTWLQQALPITLGLKFAQWLDALGRHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RRLAALRGEVLVLQFGGAAGTLASLREAAPRVAASLAAELALALPDLPWHTQRDRIAETA
HHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
AFFAMVIGTLGKIARDISLQMQTEIGELAEPAAAGKGGSSTMPHKRNPVGCAAVLSAAVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
APGLAATVFAGMVQEHERALGGWQAEWDALPELARLAGGALAGVAQIVGGLQVDTARLAA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHH
NLDATRGLVLGEAVMLALGDRIGRLDAHHLVEQASQEAVRTGRTLHEVLAAEPAVGAHLT
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PDALRRLLDPAHYVGEAAAFVDAVLVRHAGHA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MFEHSARLTSLIGGDAALIRIWSPTATLQRMLDVEAALARALAAHGVIPAAAVAPIEAAC
CCCCHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
RAERLDAEALARDAALGGNLAIPLVKQLTARVKDADAEAAKFVHWGATSQDILDTGLVLQ
HHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHCCCCCCHHHHHHHHHHH
LRETFDALALRLDTVCASLAELARVHRATPMIGRTWLQQALPITLGLKFAQWLDALGRHR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RRLAALRGEVLVLQFGGAAGTLASLREAAPRVAASLAAELALALPDLPWHTQRDRIAETA
HHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
AFFAMVIGTLGKIARDISLQMQTEIGELAEPAAAGKGGSSTMPHKRNPVGCAAVLSAAVR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCCHHHHHHHHHHH
APGLAATVFAGMVQEHERALGGWQAEWDALPELARLAGGALAGVAQIVGGLQVDTARLAA
CCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHCCCCHHHHHHHH
NLDATRGLVLGEAVMLALGDRIGRLDAHHLVEQASQEAVRTGRTLHEVLAAEPAVGAHLT
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
PDALRRLLDPAHYVGEAAAFVDAVLVRHAGHA
HHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9582432; 12597275 [H]