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The map label for this gene is pcaJ [H]

Identifier: 238023561

GI number: 238023561

Start: 65610

End: 66266

Strand: Direct

Name: pcaJ [H]

Synonym: bglu_2g00630

Alternate gene names: 238023561

Gene position: 65610-66266 (Clockwise)

Preceding gene: 238023560

Following gene: 238023562

Centisome position: 2.32

GC content: 67.88

Gene sequence:

>657_bases
ATGAAACGACTGACCCGTGATGAGATGGCCCAGCGCGTGGCCCGCGACATTCCCGAAGGCGCCTACGTGAACCTCGGCAT
CGGCGTGCCGACGCTGGTGGCGAACCACCTCGACGTGAACAAGGAAATCTTCCTGCACAGCGAGAACGGCGTGCTCGGCA
TGGGCCCCGCGCCCGAGGCCGGCGCCGAGGACGACGAGCTGATCAACGCCGGCAAGCAGCACGTCACGCTGCTGACGGGC
GGCGCGTTCTTCCACCACGCCGATTCGTTCGCGATGATGCGCGGCGGCCACCTCGACTACTGCGTGCTCGGCGCGTTCCA
GGTGTCGGCGCGCGGCGATCTGGCCAACTGGCACACCGGCGCGCCCGACGCGATCCCCGCCGTGGGCGGGGCGATGGATC
TCGCGATCGGCGCCAAGCAGGTGTTCGTGATGATGGAGCATCTGACCAAGCAGGGCGAAAGCAAGATCGTCGCCGAATGC
TCGTATCCCGTCACCGGCATCGGCTGCGTGACGCGTATCTACACCGACCTGGCCGTGCTCGACGTGACGCCGGCGGGCCT
TGCCGTGAGCGAGATCTTCACCGATCTCTCGTTCGACGAGCTGCAGGCGCTCACGGGCGTGCCGCTCGTCGACGCGACCG
AGCGCGCCACCGCGTAG

Upstream 100 bases:

>100_bases
GTGACGCCCGGCATCTTCGTGCAGCGCGTCGTCGAGGTGCCGCAGGCCGTCCATGCGGCCGAACTCGCCGCGCAGGCCGC
CTGAACCCGGAGAACCCACG

Downstream 100 bases:

>100_bases
GCGCCGGCGGCGGCCTCGCCAGCGGGCACCGGCGCGTGCCGCGGCGAGCCCGGCGCGGCGGCGATGCGCCGGGTTCGCGC
CGCGCCGCCGCTGCCTGCCG

Product: 3-oxoadipate CoA-transferase subunit B

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKRLTRDEMAQRVARDIPEGAYVNLGIGVPTLVANHLDVNKEIFLHSENGVLGMGPAPEAGAEDDELINAGKQHVTLLTG
GAFFHHADSFAMMRGGHLDYCVLGAFQVSARGDLANWHTGAPDAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVAEC
SYPVTGIGCVTRIYTDLAVLDVTPAGLAVSEIFTDLSFDELQALTGVPLVDATERATA

Sequences:

>Translated_218_residues
MKRLTRDEMAQRVARDIPEGAYVNLGIGVPTLVANHLDVNKEIFLHSENGVLGMGPAPEAGAEDDELINAGKQHVTLLTG
GAFFHHADSFAMMRGGHLDYCVLGAFQVSARGDLANWHTGAPDAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVAEC
SYPVTGIGCVTRIYTDLAVLDVTPAGLAVSEIFTDLSFDELQALTGVPLVDATERATA
>Mature_218_residues
MKRLTRDEMAQRVARDIPEGAYVNLGIGVPTLVANHLDVNKEIFLHSENGVLGMGPAPEAGAEDDELINAGKQHVTLLTG
GAFFHHADSFAMMRGGHLDYCVLGAFQVSARGDLANWHTGAPDAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVAEC
SYPVTGIGCVTRIYTDLAVLDVTPAGLAVSEIFTDLSFDELQALTGVPLVDATERATA

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI4557817, Length=203, Percent_Identity=47.2906403940887, Blast_Score=181, Evalue=4e-46,
Organism=Homo sapiens, GI11545841, Length=203, Percent_Identity=46.3054187192118, Blast_Score=177, Evalue=4e-45,
Organism=Escherichia coli, GI1788552, Length=200, Percent_Identity=42, Blast_Score=155, Evalue=3e-39,
Organism=Caenorhabditis elegans, GI17531517, Length=204, Percent_Identity=48.0392156862745, Blast_Score=186, Evalue=9e-48,
Organism=Drosophila melanogaster, GI24655794, Length=206, Percent_Identity=43.2038834951456, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21358325, Length=206, Percent_Identity=43.2038834951456, Blast_Score=168, Evalue=2e-42,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 23060; Mature: 23060

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS01274 COA_TRANSF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLTRDEMAQRVARDIPEGAYVNLGIGVPTLVANHLDVNKEIFLHSENGVLGMGPAPEA
CCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCEEEEECCCCEEECCCCCCC
GAEDDELINAGKQHVTLLTGGAFFHHADSFAMMRGGHLDYCVLGAFQVSARGDLANWHTG
CCCHHHHHHCCCCEEEEEECCHHHHHCCCHHHHCCCCCCEEEEEEEEEECCCCCCCCCCC
APDAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVAECSYPVTGIGCVTRIYTDLAVL
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHEE
DVTPAGLAVSEIFTDLSFDELQALTGVPLVDATERATA
ECCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCHHCCC
>Mature Secondary Structure
MKRLTRDEMAQRVARDIPEGAYVNLGIGVPTLVANHLDVNKEIFLHSENGVLGMGPAPEA
CCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHCCCCCEEEEECCCCEEECCCCCCC
GAEDDELINAGKQHVTLLTGGAFFHHADSFAMMRGGHLDYCVLGAFQVSARGDLANWHTG
CCCHHHHHHCCCCEEEEEECCHHHHHCCCHHHHCCCCCCEEEEEEEEEECCCCCCCCCCC
APDAIPAVGGAMDLAIGAKQVFVMMEHLTKQGESKIVAECSYPVTGIGCVTRIYTDLAVL
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHEE
DVTPAGLAVSEIFTDLSFDELQALTGVPLVDATERATA
ECCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]