Click here to switch to the map view.

The map label for this gene is iciA [H]

Identifier: 238023551

GI number: 238023551

Start: 55479

End: 56369

Strand: Direct

Name: iciA [H]

Synonym: bglu_2g00520

Alternate gene names: 238023551

Gene position: 55479-56369 (Clockwise)

Preceding gene: 238023549

Following gene: 238023554

Centisome position: 1.96

GC content: 69.02

Gene sequence:

>891_bases
ATGCTCGACTACGCGCTGCTCGAGGCACTGTCCGCGGTGATCCGGCAAGGATCGTTCGAACGCGCGGCGAAGGACCTCAA
CGTGTCGCCGTCGGCGGTATCGCAGCGGATCAAGCTGCTCGAGGAGCGGGTCGGCAGCGTGCTCGTCAAGCGCGGGCAGC
CGTGCGTGGCGACCACCTCGGGCGCGCTGCTGTGCCGGCATACGGAGCGCGTGCAACTGCTGGAGGCCGAGCTGTCCGGG
CAGGTGCCGCATCTGCCCGGCGCGGTGGGCGCGTGGCCGACGCTGCGCATCGCGGTGAACGACGACAGCGTGGCGACCTG
GTTCATCGATGCGGTCGCCCCGTTCTGCGCGCAGAGCGGCACGCTGCTCGACCTCGTGATCGACGACCAGGATTACACCG
CCGAGCGGATTCGCGACGGCAGCGTGCAGGGCGCGATCACCGCGCAGGCCGAGCCGATCCAGGGCTGCCGGTCGACGCGG
CTTGGCCGGATTCGCTATCAAGCGGTCTGTTCACCGGCGTTCCAGGCGCGCTATTTCGCCGACGGCCTCACGCGCGAGGC
GCTGCGGCGCGCGCCGTGCGTGATGTTCAGCCCGAAGGACAGCCTGCAGATGCGCTTCATCCGGCGCGCGACGCGCACCG
ATCTCGATCCGCCGAAGCACTGGATTCCACACGTGGCAGGCTATCTGCGCGCCTGCGAGACGGGGCTCGGCTGGGGGATG
TGCCCGGTTCGCATGGTCGAGCGGCAGGTGGCCTCGGGCGAGATGGTGGATCTGTCGGAGGGGCGCAGCATCGACATCGA
GCTTTACTGGCAGAGCTGGCGGCTGTCGATCGGCTGGCTGGACGAATTCAGCCGCGTGCTGAAGGCCAGTGCCGCGCAAT
GGCTGGATTGA

Upstream 100 bases:

>100_bases
TTGGGGCGGACGGTGGACTTGAGGGACGGATGCGGCTAGTCTGCGGGTTCGGGATGCATAAGAAAAGCTAAATTCCCTAA
TCTCTATGAAGGAAATCTGA

Downstream 100 bases:

>100_bases
AGGCGGGCCGAAGGCGCGGACCGCAAAAAATAAAGGCGCCTCGGGAGGCGCCTTTTGTGATCGCAGGGCCGACAGGCGTC
GGGCCTCGCTGGGCAGTGGC

Product: chromosome replication initiation inhibitor protein

Products: NA

Alternate protein names: OriC replication inhibitor [H]

Number of amino acids: Translated: 296; Mature: 296

Protein sequence:

>296_residues
MLDYALLEALSAVIRQGSFERAAKDLNVSPSAVSQRIKLLEERVGSVLVKRGQPCVATTSGALLCRHTERVQLLEAELSG
QVPHLPGAVGAWPTLRIAVNDDSVATWFIDAVAPFCAQSGTLLDLVIDDQDYTAERIRDGSVQGAITAQAEPIQGCRSTR
LGRIRYQAVCSPAFQARYFADGLTREALRRAPCVMFSPKDSLQMRFIRRATRTDLDPPKHWIPHVAGYLRACETGLGWGM
CPVRMVERQVASGEMVDLSEGRSIDIELYWQSWRLSIGWLDEFSRVLKASAAQWLD

Sequences:

>Translated_296_residues
MLDYALLEALSAVIRQGSFERAAKDLNVSPSAVSQRIKLLEERVGSVLVKRGQPCVATTSGALLCRHTERVQLLEAELSG
QVPHLPGAVGAWPTLRIAVNDDSVATWFIDAVAPFCAQSGTLLDLVIDDQDYTAERIRDGSVQGAITAQAEPIQGCRSTR
LGRIRYQAVCSPAFQARYFADGLTREALRRAPCVMFSPKDSLQMRFIRRATRTDLDPPKHWIPHVAGYLRACETGLGWGM
CPVRMVERQVASGEMVDLSEGRSIDIELYWQSWRLSIGWLDEFSRVLKASAAQWLD
>Mature_296_residues
MLDYALLEALSAVIRQGSFERAAKDLNVSPSAVSQRIKLLEERVGSVLVKRGQPCVATTSGALLCRHTERVQLLEAELSG
QVPHLPGAVGAWPTLRIAVNDDSVATWFIDAVAPFCAQSGTLLDLVIDDQDYTAERIRDGSVQGAITAQAEPIQGCRSTR
LGRIRYQAVCSPAFQARYFADGLTREALRRAPCVMFSPKDSLQMRFIRRATRTDLDPPKHWIPHVAGYLRACETGLGWGM
CPVRMVERQVASGEMVDLSEGRSIDIELYWQSWRLSIGWLDEFSRVLKASAAQWLD

Specific function: Specific inhibitor of chromosomal initiation of replication in vitro. Binds the three 13-mers in the origin (oriC) to block initiation of replication [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789283, Length=275, Percent_Identity=38.1818181818182, Blast_Score=167, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR017685
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32832; Mature: 32832

Theoretical pI: Translated: 7.31; Mature: 7.31

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDYALLEALSAVIRQGSFERAAKDLNVSPSAVSQRIKLLEERVGSVLVKRGQPCVATTS
CCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
GALLCRHTERVQLLEAELSGQVPHLPGAVGAWPTLRIAVNDDSVATWFIDAVAPFCAQSG
CCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCC
TLLDLVIDDQDYTAERIRDGSVQGAITAQAEPIQGCRSTRLGRIRYQAVCSPAFQARYFA
CEEEEEECCCCCHHHHHCCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHCCHHHHHHHHH
DGLTREALRRAPCVMFSPKDSLQMRFIRRATRTDLDPPKHWIPHVAGYLRACETGLGWGM
HHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCC
CPVRMVERQVASGEMVDLSEGRSIDIELYWQSWRLSIGWLDEFSRVLKASAAQWLD
CHHHHHHHHHHCCCEEECCCCCEEEEEEEEEEEEEEEHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLDYALLEALSAVIRQGSFERAAKDLNVSPSAVSQRIKLLEERVGSVLVKRGQPCVATTS
CCHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
GALLCRHTERVQLLEAELSGQVPHLPGAVGAWPTLRIAVNDDSVATWFIDAVAPFCAQSG
CCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCC
TLLDLVIDDQDYTAERIRDGSVQGAITAQAEPIQGCRSTRLGRIRYQAVCSPAFQARYFA
CEEEEEECCCCCHHHHHCCCCCCEEEECCCCHHHHHHHCCCCHHHHHHHCCHHHHHHHHH
DGLTREALRRAPCVMFSPKDSLQMRFIRRATRTDLDPPKHWIPHVAGYLRACETGLGWGM
HHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCC
CPVRMVERQVASGEMVDLSEGRSIDIELYWQSWRLSIGWLDEFSRVLKASAAQWLD
CHHHHHHHHHHCCCEEECCCCCEEEEEEEEEEEEEEEHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA