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The map label for this gene is dmlR [H]

Identifier: 238023516

GI number: 238023516

Start: 16142

End: 17053

Strand: Direct

Name: dmlR [H]

Synonym: bglu_2g00160

Alternate gene names: 238023516

Gene position: 16142-17053 (Clockwise)

Preceding gene: 238023514

Following gene: 238023518

Centisome position: 0.57

GC content: 71.16

Gene sequence:

>912_bases
GTGAATAAAGCCCCGAATCTGGACGATCTGCGCGTGTTCAGCGTGGTGGTGCGCCTGACGAGCTTCAGCGCGGCGGCCGA
GCGGCTCGGCGTCTCGCCCGCCTACGTCAGCAAGCGCGTGGCGCTGCTGGAGGCCCAGCTCGGCGCGCGGCTGCTGCATC
GCTCGACGCGCCGCGTGGCCGTGACCGATTCCGGCGAGCGCGTGGTGGCGTGGGCCGAGAAGATCCTCGACGACGTCGAT
CATCTGGTCGAGGACGTCTCGACCACGCGCACGGTGCCGCGCGGCACGCTGCGGATCTCGAGCAGCTTCGGCTTCGGGCG
CCACGTGCTCGCGCCGGCGCTGCTCGACTTCAATGCGCGCTATGCGCAGATCGACGTGCGGCTCGATCTGTTCGACCGGC
TGGTCGACGTGGCCGGCGAGGGCTTCGATCTCGACGTGCGGATCGGCGACGAGATCGCCGAGCACCTGATTGCCCGGCGG
CTCGCGGCGAACCATCGCGTGCTGTGTGCCTCGCCCGACTATCTCGCGGCGCATGGCACGCCGCGCCAGATCGCGGACCT
CGCCGCGCACCAGTGCCTCGCGATCAAGGAGCGCGATCACCCGTTCGGCGTCTGGCGGCTCACGCAGCGCGGTGAGACGA
CTTCGGTGAAGGTGGGCGGCGCGCTCTCGACGAACCACGGCGAGGTGGCCGTGCAATGGGCGCTGGCCGGGCGCGGGATC
GTGCTGCGCTCGATCTGGGAGGCGGGGCCGCTGCTCGCGAGCGGCGCGCTCAAGCGGGTGCTGCCTGACGTCATGCAGCC
CGCCAACGTCTGGGCCGTCTATCCGGCGCGGCTCGCCGCCTCGGCGAAGGTGCGCGTCTGCGTCGATTTCCTCGCGCAGG
CGTTCGCGCAGTGGCCCGGCGCCACCAAGTGA

Upstream 100 bases:

>100_bases
GGTGCGCGCCGACAGACCCCGGCACGCACCGCATTCGCCTAGTGTATTGATTCCGTACAGGATTAGAATTGGCATCCGGT
TAAACGACTTTCCACGAATC

Downstream 100 bases:

>100_bases
GCGGCGGCCTTGGGCGGGCGCGGTCCGCAAAGGTGAAGGTTTTCGGGAGCTTGCGCGACACGCCCGCGTGACGTGCGCCG
CGCCATGACGGCGAACTCAC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MNKAPNLDDLRVFSVVVRLTSFSAAAERLGVSPAYVSKRVALLEAQLGARLLHRSTRRVAVTDSGERVVAWAEKILDDVD
HLVEDVSTTRTVPRGTLRISSSFGFGRHVLAPALLDFNARYAQIDVRLDLFDRLVDVAGEGFDLDVRIGDEIAEHLIARR
LAANHRVLCASPDYLAAHGTPRQIADLAAHQCLAIKERDHPFGVWRLTQRGETTSVKVGGALSTNHGEVAVQWALAGRGI
VLRSIWEAGPLLASGALKRVLPDVMQPANVWAVYPARLAASAKVRVCVDFLAQAFAQWPGATK

Sequences:

>Translated_303_residues
MNKAPNLDDLRVFSVVVRLTSFSAAAERLGVSPAYVSKRVALLEAQLGARLLHRSTRRVAVTDSGERVVAWAEKILDDVD
HLVEDVSTTRTVPRGTLRISSSFGFGRHVLAPALLDFNARYAQIDVRLDLFDRLVDVAGEGFDLDVRIGDEIAEHLIARR
LAANHRVLCASPDYLAAHGTPRQIADLAAHQCLAIKERDHPFGVWRLTQRGETTSVKVGGALSTNHGEVAVQWALAGRGI
VLRSIWEAGPLLASGALKRVLPDVMQPANVWAVYPARLAASAKVRVCVDFLAQAFAQWPGATK
>Mature_303_residues
MNKAPNLDDLRVFSVVVRLTSFSAAAERLGVSPAYVSKRVALLEAQLGARLLHRSTRRVAVTDSGERVVAWAEKILDDVD
HLVEDVSTTRTVPRGTLRISSSFGFGRHVLAPALLDFNARYAQIDVRLDLFDRLVDVAGEGFDLDVRIGDEIAEHLIARR
LAANHRVLCASPDYLAAHGTPRQIADLAAHQCLAIKERDHPFGVWRLTQRGETTSVKVGGALSTNHGEVAVQWALAGRGI
VLRSIWEAGPLLASGALKRVLPDVMQPANVWAVYPARLAASAKVRVCVDFLAQAFAQWPGATK

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=297, Percent_Identity=57.9124579124579, Blast_Score=352, Evalue=2e-98,
Organism=Escherichia coli, GI1789440, Length=292, Percent_Identity=47.6027397260274, Blast_Score=285, Evalue=3e-78,
Organism=Escherichia coli, GI1786401, Length=296, Percent_Identity=33.445945945946, Blast_Score=156, Evalue=1e-39,
Organism=Escherichia coli, GI145693193, Length=292, Percent_Identity=32.1917808219178, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI1787128, Length=289, Percent_Identity=29.0657439446367, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=30.7167235494881, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1787589, Length=293, Percent_Identity=29.6928327645051, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788706, Length=193, Percent_Identity=30.0518134715026, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1789173, Length=303, Percent_Identity=27.0627062706271, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1786448, Length=257, Percent_Identity=26.4591439688716, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1788748, Length=128, Percent_Identity=33.59375, Blast_Score=65, Evalue=5e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 33001; Mature: 33001

Theoretical pI: Translated: 9.56; Mature: 9.56

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAPNLDDLRVFSVVVRLTSFSAAAERLGVSPAYVSKRVALLEAQLGARLLHRSTRRVA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VTDSGERVVAWAEKILDDVDHLVEDVSTTRTVPRGTLRISSSFGFGRHVLAPALLDFNAR
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCE
YAQIDVRLDLFDRLVDVAGEGFDLDVRIGDEIAEHLIARRLAANHRVLCASPDYLAAHGT
EEEEEEHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCEEEEECCCEEECCCC
PRQIADLAAHQCLAIKERDHPFGVWRLTQRGETTSVKVGGALSTNHGEVAVQWALAGRGI
HHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCEEEECCEEECCCCCEEEEEEECCCCH
VLRSIWEAGPLLASGALKRVLPDVMQPANVWAVYPARLAASAKVRVCVDFLAQAFAQWPG
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCC
ATK
CCC
>Mature Secondary Structure
MNKAPNLDDLRVFSVVVRLTSFSAAAERLGVSPAYVSKRVALLEAQLGARLLHRSTRRVA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEE
VTDSGERVVAWAEKILDDVDHLVEDVSTTRTVPRGTLRISSSFGFGRHVLAPALLDFNAR
EECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHCCCCE
YAQIDVRLDLFDRLVDVAGEGFDLDVRIGDEIAEHLIARRLAANHRVLCASPDYLAAHGT
EEEEEEHHHHHHHHHHHCCCCCEEEEEEHHHHHHHHHHHHHHCCCEEEEECCCEEECCCC
PRQIADLAAHQCLAIKERDHPFGVWRLTQRGETTSVKVGGALSTNHGEVAVQWALAGRGI
HHHHHHHHHHHHHEEECCCCCCEEEEEECCCCCCEEEECCEEECCCCCEEEEEEECCCCH
VLRSIWEAGPLLASGALKRVLPDVMQPANVWAVYPARLAASAKVRVCVDFLAQAFAQWPG
HHHHHHHCCCHHHHHHHHHHHHHHCCCCCEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCC
ATK
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]