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The map label for this gene is dnaN [H]

Identifier: 237814479

GI number: 237814479

Start: 4095873

End: 4096976

Strand: Reverse

Name: dnaN [H]

Synonym: GBP346_A4277

Alternate gene names: 237814479

Gene position: 4096976-4095873 (Counterclockwise)

Preceding gene: 237814480

Following gene: 237814478

Centisome position: 99.96

GC content: 63.5

Gene sequence:

>1104_bases
ATGCAACTGGTCAAGACCGAACGAGACACCCTCCTTAGGCCGCTGCAAACCGTGAGCGGTATCGTCGAACGCCGCCACAC
GTTGCCGATCCTCGCCAATCTGTTGATCACGAAGAACGGCCCGGACGTGTCGTTCCTGTCGACCGACCTCGAGCTCCAGA
TCACGACGCGCGCCGATTTCGGCGTCGGCGGCGATCAGGTCGCGACGACCGTCGCGGCCCGCAAGCTGCTCGACATCCTG
CGCGCGATGCCCGACGGCCAGGTCACGCTGTCGCTCGCCGACAAGCGCCTGACGGTCCAGTCCGGCAAGAGCCGCTTCGC
ACTGCAAACGCTCGCGGCCGACGAGTTCCCGACCGTCGCGCAATCGAAGGATTTCGGCGCAAGCCTCGCCGTTCCGCAGA
AGGCGTTCCGCCAGTTGCTCGGCATGGTGTACTTCGCGATGGCGCAGCAGGACATCCGCTACTACCTGAACGGGATGCTG
CTCGTCGTGGACGGCGACCGCCTGATGGCGGTGGCGACCGACGGCCACCGCCTCGCGTTCTCGTCGATGAAGATCGAAGG
CTCGTTCGGCCGCCAGGAAGTGATCGTGCCGCGCAAGACGATTCTCGAGCTGCAGCGCCTGCTCGAGGACATCGACGACA
CGGTCAAGATCGACATCGCGCAGACTCAGGCGAAGTTCACGTTCGGCCAGGTCGAGCTGGTGTCGAAGCTCGTCGAGGGC
AAGTTCCCGGACTTCCAGCGCGTGATTCCGAAGGCGCACAAGAACACGTTCGAGATCGGCCGCGAAGAGTTGCAGCGCTC
GCTGCAGCGCGCGGCGATCCTCACGTCCGACAAGTTCAAGGGCGTACGCTGCATCATCGCGCCCGGCCAGTTGAAGATCA
TGTCGACGAACGCCGATCAGGAAGAGGCGCAGGAAGAACTGGAAATCGCCTACCAGGGCGACACCGTCGACATCGGCTTC
AACGTCACGTATCTGCTCGACGTGCTCGCGAACCTGAAAGTCGACACCGTTCAGGTGAGCCTCGGCGACGCGAGCTCGAG
CGCCCTCATCACCGTGCCCGAGAACGACGAATTCAAGTACGTGGTGATGCCGATGCGCATCTGA

Upstream 100 bases:

>100_bases
GAGGCACAATACTGGTTTGACCGCCTGGCCGGGCGGGCCGAGGGCCCGCCCGGCCAGGCGCTGCGAGGCTTGCAGGCCGT
TAATTGAACGAAGGAACTCT

Downstream 100 bases:

>100_bases
CGCGCGCGATTCGCCGGACACACCAAGGGGCGCCACGCCCCTTTGGCGTTTTTATGGCGATTCGCACGCTTGAGCGCCGG
CGCGCGACGCCGGCCCCCGA

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGGDQVATTVAARKLLDIL
RAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVAQSKDFGASLAVPQKAFRQLLGMVYFAMAQQDIRYYLNGML
LVVDGDRLMAVATDGHRLAFSSMKIEGSFGRQEVIVPRKTILELQRLLEDIDDTVKIDIAQTQAKFTFGQVELVSKLVEG
KFPDFQRVIPKAHKNTFEIGREELQRSLQRAAILTSDKFKGVRCIIAPGQLKIMSTNADQEEAQEELEIAYQGDTVDIGF
NVTYLLDVLANLKVDTVQVSLGDASSSALITVPENDEFKYVVMPMRI

Sequences:

>Translated_367_residues
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGGDQVATTVAARKLLDIL
RAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVAQSKDFGASLAVPQKAFRQLLGMVYFAMAQQDIRYYLNGML
LVVDGDRLMAVATDGHRLAFSSMKIEGSFGRQEVIVPRKTILELQRLLEDIDDTVKIDIAQTQAKFTFGQVELVSKLVEG
KFPDFQRVIPKAHKNTFEIGREELQRSLQRAAILTSDKFKGVRCIIAPGQLKIMSTNADQEEAQEELEIAYQGDTVDIGF
NVTYLLDVLANLKVDTVQVSLGDASSSALITVPENDEFKYVVMPMRI
>Mature_367_residues
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGGDQVATTVAARKLLDIL
RAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVAQSKDFGASLAVPQKAFRQLLGMVYFAMAQQDIRYYLNGML
LVVDGDRLMAVATDGHRLAFSSMKIEGSFGRQEVIVPRKTILELQRLLEDIDDTVKIDIAQTQAKFTFGQVELVSKLVEG
KFPDFQRVIPKAHKNTFEIGREELQRSLQRAAILTSDKFKGVRCIIAPGQLKIMSTNADQEEAQEELEIAYQGDTVDIGF
NVTYLLDVLANLKVDTVQVSLGDASSSALITVPENDEFKYVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=364, Percent_Identity=43.956043956044, Blast_Score=303, Evalue=2e-83,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40680; Mature: 40680

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADF
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCEEEEECCEEEEEEECCCC
GVGGDQVATTVAARKLLDILRAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVA
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCEEHHHHCCCCCCCCC
QSKDFGASLAVPQKAFRQLLGMVYFAMAQQDIRYYLNGMLLVVDGDRLMAVATDGHRLAF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEECCCCEEEE
SSMKIEGSFGRQEVIVPRKTILELQRLLEDIDDTVKIDIAQTQAKFTFGQVELVSKLVEG
EEEEEECCCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEEHHHHHEEHHHHHHHHHHHCC
KFPDFQRVIPKAHKNTFEIGREELQRSLQRAAILTSDKFKGVRCIIAPGQLKIMSTNADQ
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEECCCCH
EEAQEELEIAYQGDTVDIGFNVTYLLDVLANLKVDTVQVSLGDASSSALITVPENDEFKY
HHHHHHHEEEECCCEEEECCHHHHHHHHHHCCEEEEEEEEECCCCCCEEEECCCCCCEEE
VVMPMRI
EEEEECC
>Mature Secondary Structure
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADF
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCEEEEECCEEEEEEECCCC
GVGGDQVATTVAARKLLDILRAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVA
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCEEHHHHCCCCCCCCC
QSKDFGASLAVPQKAFRQLLGMVYFAMAQQDIRYYLNGMLLVVDGDRLMAVATDGHRLAF
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEECCCCEEEE
SSMKIEGSFGRQEVIVPRKTILELQRLLEDIDDTVKIDIAQTQAKFTFGQVELVSKLVEG
EEEEEECCCCCCEEECCHHHHHHHHHHHHCCCCCEEEEEEHHHHHEEHHHHHHHHHHHCC
KFPDFQRVIPKAHKNTFEIGREELQRSLQRAAILTSDKFKGVRCIIAPGQLKIMSTNADQ
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCEEEEEECCCCH
EEAQEELEIAYQGDTVDIGFNVTYLLDVLANLKVDTVQVSLGDASSSALITVPENDEFKY
HHHHHHHEEEECCCEEEECCHHHHHHHHHHCCEEEEEEEEECCCCCCEEEECCCCCCEEE
VVMPMRI
EEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]