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The map label for this gene is livK [C]

Identifier: 237814333

GI number: 237814333

Start: 3957647

End: 3958786

Strand: Reverse

Name: livK [C]

Synonym: GBP346_A4131

Alternate gene names: 237814333

Gene position: 3958786-3957647 (Counterclockwise)

Preceding gene: 237814334

Following gene: 237814330

Centisome position: 96.59

GC content: 64.91

Gene sequence:

>1140_bases
ATGCAACACACGATGAAAAAGCTGGCAGGCGCGACGTTCGTCGCGGTCATGTCGCTCGCGGGGACGGCGCACGCGGATGA
CGTCAAGATCGGCTTTGCTGCGCCGATGACGGGCGCGCAGGCGCACTACGGCAAGGATATGCAGAACGGGATCGTGCTCG
CGATCGAGGACATCAACGCGACGAAGCCGACGATCGGCGGCAAGCCGGTGAAGTTCGTGCTCGACACGCAGGACGATCAG
GCCGACCCGCGCACCGGCACGACGGTCGCGCAGAAGCTCGTCGACGACGGCATCAAGGGCATGCTCGGCCACTTCAACTC
GGGCACGACGATTCCGGCTTCGCGCATCTACGCGAACGCGGGCATCCCGCAGATCGCGATGGCGACGGCGCCCGAGTACA
CGACGCAGGGCTACAAGACGACCTTCCGGATGATGACGTCCGACACGCAGCAGGGCTCGGTCGCGGGCACGTTCGCGGTC
AAGGATCTCGGCATGAAGAAGATCGCGATCGTCGACGATCGCACCGCTTACGGCCAGGGCCTTGCCGACCAGTTCGAGAA
GGCGGCGAAGGCGGCGGGCGCGACGATCGTCGATCGTGAATTCACGAACGACAAGGCTGTCGACTTCAAGGCGATCCTGA
CGAAGCTCAAGGCGACGAAGCCGGACCTCGTCTACTACGGCGGCGCGGATTCGCAGGCCGCGCCGATGGCCAAGCAGATG
AAGTCGCTCGGCGTCACGGCGCCGCTGATGGGCGGCGAGATGGTGCACACGCCGACCTTCCTGAAGATCGCGGGCGACGC
GGCCGAAGGCTCGATCGCTTCGCTCGCCGGCCTGCCGCTCGCCGAAATGCCCGGCGGCAAGGCGTACGCGGACAAGTACA
AGAAGCGCTTCGGCGAAGACGTGCAGACGTACTCGCCGTATGCGTACGACGGCGCGATGGCGATGTTCAGCGCGATGAAG
AAGGCGAACTCGACGGACCCGGCGAAGTATCTGCCGCTGCTCGCGAAGACCGACATGGCGGGCGTGACGTCGACGCACAT
CGCGTATGACGCGAAGGGCGACCTGAGGAACGGCGGCATCACGATGTACAAGGTCGAGAAGGGCGAATGGAAGCCCCTGA
AGAGCATCGGCGGCAAGTAA

Upstream 100 bases:

>100_bases
GATGACGATTGGCTGAGCAATCCTTGCCCTTCGATTCAAGAAACGGAAGCGGCAAGGCGAACCCGAAGTCGCTCCATTTA
CCCTGGTACTAGGAGAAGAT

Downstream 100 bases:

>100_bases
GCGCCGAAGGGCGTGACGCGCGCGCCGGATAGCGGCGTTCGCGGCGCGCTTCGATGTCGATGCCGGCATCGGCGAACCCC
GGGTCCGCGGCGGCGGAACC

Product: putative periplasmic substrate-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MQHTMKKLAGATFVAVMSLAGTAHADDVKIGFAAPMTGAQAHYGKDMQNGIVLAIEDINATKPTIGGKPVKFVLDTQDDQ
ADPRTGTTVAQKLVDDGIKGMLGHFNSGTTIPASRIYANAGIPQIAMATAPEYTTQGYKTTFRMMTSDTQQGSVAGTFAV
KDLGMKKIAIVDDRTAYGQGLADQFEKAAKAAGATIVDREFTNDKAVDFKAILTKLKATKPDLVYYGGADSQAAPMAKQM
KSLGVTAPLMGGEMVHTPTFLKIAGDAAEGSIASLAGLPLAEMPGGKAYADKYKKRFGEDVQTYSPYAYDGAMAMFSAMK
KANSTDPAKYLPLLAKTDMAGVTSTHIAYDAKGDLRNGGITMYKVEKGEWKPLKSIGGK

Sequences:

>Translated_379_residues
MQHTMKKLAGATFVAVMSLAGTAHADDVKIGFAAPMTGAQAHYGKDMQNGIVLAIEDINATKPTIGGKPVKFVLDTQDDQ
ADPRTGTTVAQKLVDDGIKGMLGHFNSGTTIPASRIYANAGIPQIAMATAPEYTTQGYKTTFRMMTSDTQQGSVAGTFAV
KDLGMKKIAIVDDRTAYGQGLADQFEKAAKAAGATIVDREFTNDKAVDFKAILTKLKATKPDLVYYGGADSQAAPMAKQM
KSLGVTAPLMGGEMVHTPTFLKIAGDAAEGSIASLAGLPLAEMPGGKAYADKYKKRFGEDVQTYSPYAYDGAMAMFSAMK
KANSTDPAKYLPLLAKTDMAGVTSTHIAYDAKGDLRNGGITMYKVEKGEWKPLKSIGGK
>Mature_379_residues
MQHTMKKLAGATFVAVMSLAGTAHADDVKIGFAAPMTGAQAHYGKDMQNGIVLAIEDINATKPTIGGKPVKFVLDTQDDQ
ADPRTGTTVAQKLVDDGIKGMLGHFNSGTTIPASRIYANAGIPQIAMATAPEYTTQGYKTTFRMMTSDTQQGSVAGTFAV
KDLGMKKIAIVDDRTAYGQGLADQFEKAAKAAGATIVDREFTNDKAVDFKAILTKLKATKPDLVYYGGADSQAAPMAKQM
KSLGVTAPLMGGEMVHTPTFLKIAGDAAEGSIASLAGLPLAEMPGGKAYADKYKKRFGEDVQTYSPYAYDGAMAMFSAMK
KANSTDPAKYLPLLAKTDMAGVTSTHIAYDAKGDLRNGGITMYKVEKGEWKPLKSIGGK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=355, Percent_Identity=33.8028169014084, Blast_Score=171, Evalue=8e-44,
Organism=Escherichia coli, GI48994941, Length=341, Percent_Identity=31.3782991202346, Blast_Score=154, Evalue=8e-39,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 40028; Mature: 40028

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHTMKKLAGATFVAVMSLAGTAHADDVKIGFAAPMTGAQAHYGKDMQNGIVLAIEDINA
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHCCCCCCCCEEEEEECCCC
TKPTIGGKPVKFVLDTQDDQADPRTGTTVAQKLVDDGIKGMLGHFNSGTTIPASRIYANA
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEECC
GIPQIAMATAPEYTTQGYKTTFRMMTSDTQQGSVAGTFAVKDLGMKKIAIVDDRTAYGQG
CCCCEEEECCCCHHCCCHHEEEEEEECCCCCCCEEEEEEEHHCCCEEEEEEECCCHHCCC
LADQFEKAAKAAGATIVDREFTNDKAVDFKAILTKLKATKPDLVYYGGADSQAAPMAKQM
HHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHH
KSLGVTAPLMGGEMVHTPTFLKIAGDAAEGSIASLAGLPLAEMPGGKAYADKYKKRFGED
HHCCCCCCCCCCCEECCCEEEEEECCCCCCCHHHHHCCCHHCCCCCHHHHHHHHHHHCCC
VQTYSPYAYDGAMAMFSAMKKANSTDPAKYLPLLAKTDMAGVTSTHIAYDAKGDLRNGGI
HHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCE
TMYKVEKGEWKPLKSIGGK
EEEEECCCCCCHHHHCCCC
>Mature Secondary Structure
MQHTMKKLAGATFVAVMSLAGTAHADDVKIGFAAPMTGAQAHYGKDMQNGIVLAIEDINA
CCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCHHHCCCCCCCCEEEEEECCCC
TKPTIGGKPVKFVLDTQDDQADPRTGTTVAQKLVDDGIKGMLGHFNSGTTIPASRIYANA
CCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEECC
GIPQIAMATAPEYTTQGYKTTFRMMTSDTQQGSVAGTFAVKDLGMKKIAIVDDRTAYGQG
CCCCEEEECCCCHHCCCHHEEEEEEECCCCCCCEEEEEEEHHCCCEEEEEEECCCHHCCC
LADQFEKAAKAAGATIVDREFTNDKAVDFKAILTKLKATKPDLVYYGGADSQAAPMAKQM
HHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCHHHHHHH
KSLGVTAPLMGGEMVHTPTFLKIAGDAAEGSIASLAGLPLAEMPGGKAYADKYKKRFGED
HHCCCCCCCCCCCEECCCEEEEEECCCCCCCHHHHHCCCHHCCCCCHHHHHHHHHHHCCC
VQTYSPYAYDGAMAMFSAMKKANSTDPAKYLPLLAKTDMAGVTSTHIAYDAKGDLRNGGI
HHHCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCE
TMYKVEKGEWKPLKSIGGK
EEEEECCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA