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The map label for this gene is livK [C]

Identifier: 237814222

GI number: 237814222

Start: 3848155

End: 3849297

Strand: Reverse

Name: livK [C]

Synonym: GBP346_A4017

Alternate gene names: 237814222

Gene position: 3849297-3848155 (Counterclockwise)

Preceding gene: 237814223

Following gene: 237814220

Centisome position: 93.92

GC content: 64.65

Gene sequence:

>1143_bases
ATGAATATCAAGATGCAAAAGCTGCTGCCGATCAGCGCGGCGGCTATGCTGTTGGCCGCAGCCGCCACGAACGCTGCCGC
CGACCAGGTCGTCAAGATCGGCCATGTCGCACCGTTGACGGGCGGGATCGCCCACCTTGGTAAGGACAACGAAAATGGTG
CGCGCCTCGCGGTCGAGGAGATCAACGCGAAGGGACTCACGATCGGCGGCCAGAAGGTGACGCTCCAGCTCGATGCACAG
GACGACGCGGCCGACCCGCGCACCGCGACGCAGGTCGCGCAGAAGCTCGTCGACGACAAGGTCGTCGCGGTCGTCGGCCA
CCTGAACTCGGGTACCTCGATTCCGGCCTCGAAGATCTACAGCGATGCGGGCATCGTGCAGATCTCGCCGTCGGCGACGA
ACCCGGCTTACACGCAGCAAGGTTTCAAGACGACCTACCGCGTCGTCGCGACCGACGCGCAGCAGGGCCCGGCGCTCGCG
AACTACGCGCGCTCGAAGGGCGTGAAGAGCGTCGCCGTCGTCGACGATTCGACGGCCTACGGCCAGGGCCTCGCGAACGA
ATTCGAGAAGAAGGCGAAGGCGCTCGGCCTGAAGGTGGTGTCGCACGACGCGACGAACGACAAGGCGGTCGACTTCCGCG
CGATCCTGACGAAGATCAAGGGTGAAAATCCGGACGCCGTGATGTACGGCGGCATGGACGCGACGGGCGGCCCGTTCGCG
AAGCAGGCGAAGCAGCTCGGCCTGCGCGCGAAGATCTTCGCGGGCGACGGCGTCTGCACGGAAAAGCTCGCGGACCTCGC
GGGCGATGCGACCGACAACGTCGTGTGCTCGGAGGCGGGCGCGTCGCTCGAGAAGATGCCGGGCGGCGCGGCATTCAAGG
CGAAGTACGAGAAGCGTTTCGGCCAGCCGATCCAGATCTACGCGCCGTTCACGTATGACGCGGTGTACATCATCGTCGAC
GCGATGAAGCGCGCGAACTCGACGGATCCGGCGAAGATCGTCGCGGCGATGCCGAAGACGAACTACACCGGCGTGATCGG
CACGACGACCTTCGATTCGAAGGGCGACCTGCAGCACGGCGTGATCTCGCTGTACGACTACAAGGGCGGCAAGAAGACGT
TCCTGGATGAAGTGAAGATGTAA

Upstream 100 bases:

>100_bases
AATGCAACCGGCGAATGTCCAGTGATTTTTTGTCGAAACACGAGGATCGGTGCGGGGCGTCGGTGAAAGCGTTTTCTTGG
GATTGAAACTGGAGACTTAC

Downstream 100 bases:

>100_bases
TACCGTCTGCACGGCCGACGGAGGGGGCGGGGCGCGCATGCCGTCAGGCATGCGCGCCTCGTTTTTTTTCCGCGAAGAAA
GCGTGCGATGCGCGGCGCAG

Product: putative amino acid ABC transporter, periplasmic amino acid-binding protein

Products: ADP; phosphate; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MNIKMQKLLPISAAAMLLAAAATNAAADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEEINAKGLTIGGQKVTLQLDAQ
DDAADPRTATQVAQKLVDDKVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALA
NYARSKGVKSVAVVDDSTAYGQGLANEFEKKAKALGLKVVSHDATNDKAVDFRAILTKIKGENPDAVMYGGMDATGGPFA
KQAKQLGLRAKIFAGDGVCTEKLADLAGDATDNVVCSEAGASLEKMPGGAAFKAKYEKRFGQPIQIYAPFTYDAVYIIVD
AMKRANSTDPAKIVAAMPKTNYTGVIGTTTFDSKGDLQHGVISLYDYKGGKKTFLDEVKM

Sequences:

>Translated_380_residues
MNIKMQKLLPISAAAMLLAAAATNAAADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEEINAKGLTIGGQKVTLQLDAQ
DDAADPRTATQVAQKLVDDKVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALA
NYARSKGVKSVAVVDDSTAYGQGLANEFEKKAKALGLKVVSHDATNDKAVDFRAILTKIKGENPDAVMYGGMDATGGPFA
KQAKQLGLRAKIFAGDGVCTEKLADLAGDATDNVVCSEAGASLEKMPGGAAFKAKYEKRFGQPIQIYAPFTYDAVYIIVD
AMKRANSTDPAKIVAAMPKTNYTGVIGTTTFDSKGDLQHGVISLYDYKGGKKTFLDEVKM
>Mature_380_residues
MNIKMQKLLPISAAAMLLAAAATNAAADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEEINAKGLTIGGQKVTLQLDAQ
DDAADPRTATQVAQKLVDDKVVAVVGHLNSGTSIPASKIYSDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALA
NYARSKGVKSVAVVDDSTAYGQGLANEFEKKAKALGLKVVSHDATNDKAVDFRAILTKIKGENPDAVMYGGMDATGGPFA
KQAKQLGLRAKIFAGDGVCTEKLADLAGDATDNVVCSEAGASLEKMPGGAAFKAKYEKRFGQPIQIYAPFTYDAVYIIVD
AMKRANSTDPAKIVAAMPKTNYTGVIGTTTFDSKGDLQHGVISLYDYKGGKKTFLDEVKM

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1789867, Length=346, Percent_Identity=34.6820809248555, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI48994941, Length=368, Percent_Identity=32.6086956521739, Blast_Score=177, Evalue=9e-46,

Paralogues:

None

Copy number: 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39761; Mature: 39761

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIKMQKLLPISAAAMLLAAAATNAAADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEE
CCCCHHHHCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCEEEEEE
INAKGLTIGGQKVTLQLDAQDDAADPRTATQVAQKLVDDKVVAVVGHLNSGTSIPASKIY
CCCCCEEECCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHH
SDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALANYARSKGVKSVAVVDDSTAY
CCCCEEEECCCCCCCCHHHHCCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCHH
GQGLANEFEKKAKALGLKVVSHDATNDKAVDFRAILTKIKGENPDAVMYGGMDATGGPFA
CCHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHH
KQAKQLGLRAKIFAGDGVCTEKLADLAGDATDNVVCSEAGASLEKMPGGAAFKAKYEKRF
HHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCCEEECCCCCCHHHCCCCCHHHHHHHHHC
GQPIQIYAPFTYDAVYIIVDAMKRANSTDPAKIVAAMPKTNYTGVIGTTTFDSKGDLQHG
CCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHEEEECCCCCCCEEEEECCCCCCCCCCCC
VISLYDYKGGKKTFLDEVKM
EEEEEECCCCCHHHHHHHCC
>Mature Secondary Structure
MNIKMQKLLPISAAAMLLAAAATNAAADQVVKIGHVAPLTGGIAHLGKDNENGARLAVEE
CCCCHHHHCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCEEEEEE
INAKGLTIGGQKVTLQLDAQDDAADPRTATQVAQKLVDDKVVAVVGHLNSGTSIPASKIY
CCCCCEEECCEEEEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHH
SDAGIVQISPSATNPAYTQQGFKTTYRVVATDAQQGPALANYARSKGVKSVAVVDDSTAY
CCCCEEEECCCCCCCCHHHHCCCEEEEEEEECCCCCCHHHHHHHHCCCCEEEEEECCCHH
GQGLANEFEKKAKALGLKVVSHDATNDKAVDFRAILTKIKGENPDAVMYGGMDATGGPFA
CCHHHHHHHHHHHHHCEEEEECCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHH
KQAKQLGLRAKIFAGDGVCTEKLADLAGDATDNVVCSEAGASLEKMPGGAAFKAKYEKRF
HHHHHCCCEEEEEECCCHHHHHHHHHCCCCCCCEEECCCCCCHHHCCCCCHHHHHHHHHC
GQPIQIYAPFTYDAVYIIVDAMKRANSTDPAKIVAAMPKTNYTGVIGTTTFDSKGDLQHG
CCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHEEEECCCCCCCEEEEECCCCCCCCCCCC
VISLYDYKGGKKTFLDEVKM
EEEEEECCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA