Click here to switch to the map view.

The map label for this gene is rhtB [H]

Identifier: 237814194

GI number: 237814194

Start: 3828951

End: 3829619

Strand: Reverse

Name: rhtB [H]

Synonym: GBP346_A3988

Alternate gene names: 237814194

Gene position: 3829619-3828951 (Counterclockwise)

Preceding gene: 237814195

Following gene: 237814192

Centisome position: 93.44

GC content: 68.31

Gene sequence:

>669_bases
ATGAATCTGCATACGTGGTGGCTGTTCGTCGCGACGGTGTTCGTCGTGTCGGCGATTCCCGGGCCGAACATGTTGCTCGT
GATGACGCACGGTGCGCGTCACGGGCTACGGCGTTCGACGGCGACGATGGCGGGCTGCATGACGGCGCTTGTCGCGATGC
TGTCGGTGTCCGCCGCGGGCCTCGGCGTGTTCCTCGAGGCGTGGCCCGCGATGTTCGATGCACTGCGTTTCGCGGGCGCC
GCGTATCTGATCTATCTGGGCGTGAAGGCATGGCGCGCGCGCGTCGACGAGCCGTCCGATGCGCAATCCAATGCGCACGC
CGAGGCCGCGGCGCCGCGCGCGGGCCGTTCGGGCTCGGCGGGCCAGGGGTCTCGTTGGGCGCTCTTTCGCAACGGCTTTC
TCGTCGCGGGCAGCAATCCGAAGGCGATCCTGTTCGCCGCCGCGCTGCTGCCGCAGTTCATCGACGCTTCCGCGCCGACG
CTGCCGCAGTTCGGCATCCTCGTCGCGACGTTCGCGGTGATCGAGGTGAGTTGGTACATCGTCTACGCGTCGTTCGGCAC
GCGGATCGGCGTGACGCTGCGCAGCGCGAACGTCGCGAAGGCGTTCAATCGGCTGACGGGCGGCCTGTTCGTCGGCTTCG
GCGCGATGATGGCGCTCGTGCGGCACTGA

Upstream 100 bases:

>100_bases
GACGCGCGCGGTGCATCGTGCCGCCGTCCCGCCGCAGACAGCGACACGCATTCCCCGGCATAATCGGCGTTTCCATTTCA
TTTACGGCAGCGGGGCGGCA

Downstream 100 bases:

>100_bases
AACACGCGCGCGGCAGGCTCGGCGCGCCCCGCCGCGCCGCATGAACGAATGAAACGCCCCGCGTCGGTGACGGCGCGGGG
CGTCGTCGTTTCAGCGGGGC

Product: translocator protein, LysE family

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTATMAGCMTALVAMLSVSAAGLGVFLEAWPAMFDALRFAGA
AYLIYLGVKAWRARVDEPSDAQSNAHAEAAAPRAGRSGSAGQGSRWALFRNGFLVAGSNPKAILFAAALLPQFIDASAPT
LPQFGILVATFAVIEVSWYIVYASFGTRIGVTLRSANVAKAFNRLTGGLFVGFGAMMALVRH

Sequences:

>Translated_222_residues
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTATMAGCMTALVAMLSVSAAGLGVFLEAWPAMFDALRFAGA
AYLIYLGVKAWRARVDEPSDAQSNAHAEAAAPRAGRSGSAGQGSRWALFRNGFLVAGSNPKAILFAAALLPQFIDASAPT
LPQFGILVATFAVIEVSWYIVYASFGTRIGVTLRSANVAKAFNRLTGGLFVGFGAMMALVRH
>Mature_222_residues
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTATMAGCMTALVAMLSVSAAGLGVFLEAWPAMFDALRFAGA
AYLIYLGVKAWRARVDEPSDAQSNAHAEAAAPRAGRSGSAGQGSRWALFRNGFLVAGSNPKAILFAAALLPQFIDASAPT
LPQFGILVATFAVIEVSWYIVYASFGTRIGVTLRSANVAKAFNRLTGGLFVGFGAMMALVRH

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: COG1280

COG function: function code E; Putative threonine efflux protein

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=224, Percent_Identity=33.9285714285714, Blast_Score=119, Evalue=1e-28,
Organism=Escherichia coli, GI1788099, Length=223, Percent_Identity=30.4932735426009, Blast_Score=97, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 23534; Mature: 23534

Theoretical pI: Translated: 11.34; Mature: 11.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTATMAGCMTALVAMLSVSAAG
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVFLEAWPAMFDALRFAGAAYLIYLGVKAWRARVDEPSDAQSNAHAEAAAPRAGRSGSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCC
GQGSRWALFRNGFLVAGSNPKAILFAAALLPQFIDASAPTLPQFGILVATFAVIEVSWYI
CCCCEEEEEECCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEE
VYASFGTRIGVTLRSANVAKAFNRLTGGLFVGFGAMMALVRH
EEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNLHTWWLFVATVFVVSAIPGPNMLLVMTHGARHGLRRSTATMAGCMTALVAMLSVSAAG
CCCHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVFLEAWPAMFDALRFAGAAYLIYLGVKAWRARVDEPSDAQSNAHAEAAAPRAGRSGSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCCCCCCC
GQGSRWALFRNGFLVAGSNPKAILFAAALLPQFIDASAPTLPQFGILVATFAVIEVSWYI
CCCCEEEEEECCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHEE
VYASFGTRIGVTLRSANVAKAFNRLTGGLFVGFGAMMALVRH
EEEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]