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The map label for this gene is hisF [H]

Identifier: 237814060

GI number: 237814060

Start: 3697650

End: 3698438

Strand: Reverse

Name: hisF [H]

Synonym: GBP346_A3845

Alternate gene names: 237814060

Gene position: 3698438-3697650 (Counterclockwise)

Preceding gene: 237814061

Following gene: 237814059

Centisome position: 90.24

GC content: 69.96

Gene sequence:

>789_bases
ATGCCGCGCAACATCATGGCTCTAGCTAAACGCATCATCCCCTGTCTCGACGTGACCGCCGGGCGCGTCGTCAAGGGAGT
CAATTTCGTCGAGCTGCGCGACGCGGGAGACCCCGTCGAGATCGCGCGGCGCTATGACGCCCAGGGCGCGGACGAACTCA
CGTTCCTCGACATCACCGCGACGTCGGACGGCCGCGACCTGATCCTGCCGATCATCGAGGCGGTCGCGTCGCAGGTGTTC
ATCCCGCTCACGGTGGGCGGCGGCGTGCGCGCGGTCGAGGACGTGCGGCGCCTCCTGAACGCCGGCGCGGACAAGGTCAG
CATGAACTCGTCGGCGGTCGCCAATCCGCCGCTCGTGCGCGACGCGGCCGATAAGTACGGCTCGCAGTGCATCGTCGTCG
CGATCGACGCGAAGCGCGTGTCGGCCGACGGCGAGCCGCCGCGCTGGGAAGTCTTCACGCACGGCGGCCGCAAGGGCACG
GGCCTCGACGCGGTCGAATGGGCGCGCAAGATGGCCGAGCTCGGCGCGGGCGAGATCCTGCTCACGAGCATGGACCGCGA
CGGCACGAAGGCGGGTTTCGATCTCGCGCTCACGCGCGCGGTGTCGGATGCGGTGCCGGTGCCCGTGATCGCGTCGGGCG
GCGTCGGCTCGCTCGAGCATCTCGCGGCGGGCGTCACCGAAGGCCACGCGGACGCCGTGCTCGCCGCGAGCATCTTCCAT
TACGGCGAGCACACGGTCGGCGAGGCGAAGCGCTTCATGGCCGAGCGCGGCATTGCAGTGAGGTTGTGA

Upstream 100 bases:

>100_bases
GACGAACTGAACGGCGAGCTCGACGACGCCTGACGCGCCGTCTCGGCACGACGCCTTTTCGCCAAGCCGTCCGCCTCGCG
CGGGCGGCATTACCCGCGGC

Downstream 100 bases:

>100_bases
TGAATGCAGAAGCGAAGCCGGGCGACTGGCTGGGCAAGGTCCGCTGGGACGCGAACGGCCTCGTGCCGGTGATCGCGCAG
GACGCGGCGACGAACGACGT

Product: imidazole glycerol phosphate synthase subunit HisF

Products: D-erythro-imidazole-glycerol-phosphate; AICAR; L-glutamate [C]

Alternate protein names: IGP synthase cyclase subunit; IGP synthase subunit hisF; ImGP synthase subunit hisF; IGPS subunit hisF [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MPRNIMALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDAQGADELTFLDITATSDGRDLILPIIEAVASQVF
IPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPPLVRDAADKYGSQCIVVAIDAKRVSADGEPPRWEVFTHGGRKGT
GLDAVEWARKMAELGAGEILLTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGVTEGHADAVLAASIFH
YGEHTVGEAKRFMAERGIAVRL

Sequences:

>Translated_262_residues
MPRNIMALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDAQGADELTFLDITATSDGRDLILPIIEAVASQVF
IPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPPLVRDAADKYGSQCIVVAIDAKRVSADGEPPRWEVFTHGGRKGT
GLDAVEWARKMAELGAGEILLTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGVTEGHADAVLAASIFH
YGEHTVGEAKRFMAERGIAVRL
>Mature_261_residues
PRNIMALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDAQGADELTFLDITATSDGRDLILPIIEAVASQVFI
PLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPPLVRDAADKYGSQCIVVAIDAKRVSADGEPPRWEVFTHGGRKGTG
LDAVEWARKMAELGAGEILLTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGVTEGHADAVLAASIFHY
GEHTVGEAKRFMAERGIAVRL

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the hisH subunit [H]

COG id: COG0107

COG function: function code E; Imidazoleglycerol-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI1788336, Length=259, Percent_Identity=42.8571428571429, Blast_Score=214, Evalue=5e-57,
Organism=Escherichia coli, GI87082028, Length=242, Percent_Identity=32.2314049586777, Blast_Score=105, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6319725, Length=316, Percent_Identity=35.4430379746835, Blast_Score=170, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR004651
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: 4.1.3.- [C]

Molecular weight: Translated: 27577; Mature: 27446

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPRNIMALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDAQGADELTFLDITA
CCCHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEE
TSDGRDLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPPLVR
CCCCCCHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHH
DAADKYGSQCIVVAIDAKRVSADGEPPRWEVFTHGGRKGTGLDAVEWARKMAELGAGEIL
HHHHHHCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEE
LTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGVTEGHADAVLAASIFH
EEECCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHH
YGEHTVGEAKRFMAERGIAVRL
HCCCCHHHHHHHHHHCCCEEEC
>Mature Secondary Structure 
PRNIMALAKRIIPCLDVTAGRVVKGVNFVELRDAGDPVEIARRYDAQGADELTFLDITA
CCHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCHHHHHHHHCCCCCCCEEEEEEEE
TSDGRDLILPIIEAVASQVFIPLTVGGGVRAVEDVRRLLNAGADKVSMNSSAVANPPLVR
CCCCCCHHHHHHHHHHHCEEEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCCCCCCCHH
DAADKYGSQCIVVAIDAKRVSADGEPPRWEVFTHGGRKGTGLDAVEWARKMAELGAGEIL
HHHHHHCCEEEEEEEECEEECCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEE
LTSMDRDGTKAGFDLALTRAVSDAVPVPVIASGGVGSLEHLAAGVTEGHADAVLAASIFH
EEECCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHCCCCCCHHHHHHHHHHH
YGEHTVGEAKRFMAERGIAVRL
HCCCCHHHHHHHHHHCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: phosphoribulosylformimino-AICAR-P; L-glutamine [C]

Specific reaction: phosphoribulosylformimino-AICAR-P + L-glutamine = D-erythro-imidazole-glycerol-phosphate + AICAR + L-glutamate [C]

General reaction: Lyases; Carbon-Nitrogen Lyases; Amidine-Lyases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA