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The map label for this gene is cyaE [H]

Identifier: 237814010

GI number: 237814010

Start: 3655294

End: 3656742

Strand: Direct

Name: cyaE [H]

Synonym: GBP346_A3794

Alternate gene names: 237814010

Gene position: 3655294-3656742 (Clockwise)

Preceding gene: 237814009

Following gene: 237814011

Centisome position: 89.18

GC content: 68.74

Gene sequence:

>1449_bases
ATGATCGCGATGAAGCCCGCACGCACCCTCGCCATGGCCGGCGCGCTCGCGCTCTTCGCATCGCAAGCGGCGCACGCGCA
ATGGCTCGATCTCTACGGAACCCGAAAGAATGTGTCGGCTTCTCCCGCCGCGCCGATGCTGAAGAACGCGACATGCCAGC
CCGAGCTCGCGGACCGTCCGATCGAGCTCGAAGACGCGATCCTGCAGGCGATCTGCGCGAACCCGCAGGCACGGCGCGCA
TGGGCCGATGCGCGCGCGCAGGCCGCCCAGCTCGGCGTGAAGGAGGCGGCGTATCTGCCGACGCTCAATGCAACAGCGGG
CATTCAGCGCAACTGGCAAACGACGACGTATGAGCAGGACCTCGGCCCGATCACGCTCACCGCCGACCAGAAGCAGATCC
AGACGAGCCGCTACGGCGCGTTGAACCTGAGCTGGGTGCTGTTCGATTTCGGCAAGCGCAGCGCGGCGCTCAGGCAGGCG
CGCGAGCTCCTCGCCGCCGCCAACGCGACGCAGGACGACACGCTCCAGACCATCCTGTTCAATGCCGCCCAGGCGTATTA
CAACCTGCGCGACACGCAGGCGGCGCTTGACGCGGCGCGCACGACCGAACGCGTCGCGCAGGATAGCCTCACCGCGGCGA
AGGCGAAGCACGACGCCGGCGCCGGCACGCTGAGCGATCAACTGCAGGCGCAAACGAGCTATCGCCGCGCGGTGCTCGAT
CGCGTGAGCGCCGAAGGCGACGTGCGGAACGCGACGGGCGTGCTCGCCGTCGCGATGGGGCTCGACGCGAACACGCCGCT
GCGGATCGCGACCGCCGAGCCGCCGGTCGATCGCAACGCGTTCGCCGAAGGCATCGCGCAATTGATCGACGAGGCGAAGC
GGCAGCATCCGAAGCTCGTCGCCGCGCGCGCGAAACTCGACGCGGCGCGCGCGAACGTCGATGCGGTGCGTGCGCAGGGC
CGCCCGACCATCTCGCTGACCGGCAGCCTCAGCCGCAACAGCCCGTCGTATCAGCAGCAGCCGGGCATCCAGACCACCGC
GAGCCACGGCAGCATGATCGGCGTGCAGGTGACGATCCCGCTCTTCGAAGGCTTCGCGTCCGGCTACCGCGTCGCGGCCG
CGCAGGCACAGGCGGATGCGCAGGAGGCCGACGTGCAGAACACCGAGCTGAACGTCTCGCTCGACGTGTGGAAGAGCTAT
CAAAGCCTGCAGACCGACACCGCGAATCTCGACAATTCGAAGGACCTGCTCGGCGATGCGCAACGCTCGCTCGACATCGC
ACGGGGCCGGTACAAGGCGGGCGTCGGCACGTTCACGGAGTTGCTCAATGCGCAGACCGCGCTCGCGGACGCGCAGAAGC
AGCGCGTGCAGGCGCTGTCGAAATGGCGCACCGCGCGGCTCAAGCTCGCGGCGAGCCTCGGCAGCATCGGCCTGTGGTCC
GCCCATTGA

Upstream 100 bases:

>100_bases
CGGCGCCGGCTTCCGCCGAGGCACAGGCGGCGCCCGCTGCGGCCTGCGCCGCTCCCGCCGCGCCGCCCGCGCAAGCCGCT
ACGCAACCGGCCACGGGGCG

Downstream 100 bases:

>100_bases
ACGATGCGCGAACACAAAGGCGAAGGCGAACGCTCCTCGCGCTCGCGCACGGAACGATGAAGCGACCGGCGAAGGCGGCC
CATGCGGCGACGCCCGCCGC

Product: type I secretion outer membrane protein, TolC family

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 482

Protein sequence:

>482_residues
MIAMKPARTLAMAGALALFASQAAHAQWLDLYGTRKNVSASPAAPMLKNATCQPELADRPIELEDAILQAICANPQARRA
WADARAQAAQLGVKEAAYLPTLNATAGIQRNWQTTTYEQDLGPITLTADQKQIQTSRYGALNLSWVLFDFGKRSAALRQA
RELLAAANATQDDTLQTILFNAAQAYYNLRDTQAALDAARTTERVAQDSLTAAKAKHDAGAGTLSDQLQAQTSYRRAVLD
RVSAEGDVRNATGVLAVAMGLDANTPLRIATAEPPVDRNAFAEGIAQLIDEAKRQHPKLVAARAKLDAARANVDAVRAQG
RPTISLTGSLSRNSPSYQQQPGIQTTASHGSMIGVQVTIPLFEGFASGYRVAAAQAQADAQEADVQNTELNVSLDVWKSY
QSLQTDTANLDNSKDLLGDAQRSLDIARGRYKAGVGTFTELLNAQTALADAQKQRVQALSKWRTARLKLAASLGSIGLWS
AH

Sequences:

>Translated_482_residues
MIAMKPARTLAMAGALALFASQAAHAQWLDLYGTRKNVSASPAAPMLKNATCQPELADRPIELEDAILQAICANPQARRA
WADARAQAAQLGVKEAAYLPTLNATAGIQRNWQTTTYEQDLGPITLTADQKQIQTSRYGALNLSWVLFDFGKRSAALRQA
RELLAAANATQDDTLQTILFNAAQAYYNLRDTQAALDAARTTERVAQDSLTAAKAKHDAGAGTLSDQLQAQTSYRRAVLD
RVSAEGDVRNATGVLAVAMGLDANTPLRIATAEPPVDRNAFAEGIAQLIDEAKRQHPKLVAARAKLDAARANVDAVRAQG
RPTISLTGSLSRNSPSYQQQPGIQTTASHGSMIGVQVTIPLFEGFASGYRVAAAQAQADAQEADVQNTELNVSLDVWKSY
QSLQTDTANLDNSKDLLGDAQRSLDIARGRYKAGVGTFTELLNAQTALADAQKQRVQALSKWRTARLKLAASLGSIGLWS
AH
>Mature_482_residues
MIAMKPARTLAMAGALALFASQAAHAQWLDLYGTRKNVSASPAAPMLKNATCQPELADRPIELEDAILQAICANPQARRA
WADARAQAAQLGVKEAAYLPTLNATAGIQRNWQTTTYEQDLGPITLTADQKQIQTSRYGALNLSWVLFDFGKRSAALRQA
RELLAAANATQDDTLQTILFNAAQAYYNLRDTQAALDAARTTERVAQDSLTAAKAKHDAGAGTLSDQLQAQTSYRRAVLD
RVSAEGDVRNATGVLAVAMGLDANTPLRIATAEPPVDRNAFAEGIAQLIDEAKRQHPKLVAARAKLDAARANVDAVRAQG
RPTISLTGSLSRNSPSYQQQPGIQTTASHGSMIGVQVTIPLFEGFASGYRVAAAQAQADAQEADVQNTELNVSLDVWKSY
QSLQTDTANLDNSKDLLGDAQRSLDIARGRYKAGVGTFTELLNAQTALADAQKQRVQALSKWRTARLKLAASLGSIGLWS
AH

Specific function: CyaE is necessary for transport of calmodulin-sensitive adenylate cyclase-hemolysin (cyclolysin) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=423, Percent_Identity=21.9858156028369, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51615; Mature: 51615

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAMKPARTLAMAGALALFASQAAHAQWLDLYGTRKNVSASPAAPMLKNATCQPELADRP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCHHCCCC
IELEDAILQAICANPQARRAWADARAQAAQLGVKEAAYLPTLNATAGIQRNWQTTTYEQD
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCC
LGPITLTADQKQIQTSRYGALNLSWVLFDFGKRSAALRQARELLAAANATQDDTLQTILF
CCCEEEECCHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
NAAQAYYNLRDTQAALDAARTTERVAQDSLTAAKAKHDAGAGTLSDQLQAQTSYRRAVLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
RVSAEGDVRNATGVLAVAMGLDANTPLRIATAEPPVDRNAFAEGIAQLIDEAKRQHPKLV
HHCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHH
AARAKLDAARANVDAVRAQGRPTISLTGSLSRNSPSYQQQPGIQTTASHGSMIGVQVTIP
HHHHHHHHHHCCHHHHHCCCCCEEEEEECCCCCCCCHHHCCCCCEECCCCCEEEEEEEEH
LFEGFASGYRVAAAQAQADAQEADVQNTELNVSLDVWKSYQSLQTDTANLDNSKDLLGDA
HHHHHHCCCEEEHHHHHCCHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHH
QRSLDIARGRYKAGVGTFTELLNAQTALADAQKQRVQALSKWRTARLKLAASLGSIGLWS
HHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AH
CC
>Mature Secondary Structure
MIAMKPARTLAMAGALALFASQAAHAQWLDLYGTRKNVSASPAAPMLKNATCQPELADRP
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHCCCCCCCHHCCCC
IELEDAILQAICANPQARRAWADARAQAAQLGVKEAAYLPTLNATAGIQRNWQTTTYEQD
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCCCCCCCCCCCCCC
LGPITLTADQKQIQTSRYGALNLSWVLFDFGKRSAALRQARELLAAANATQDDTLQTILF
CCCEEEECCHHHHHHHHCCCEEEEEEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHHHHH
NAAQAYYNLRDTQAALDAARTTERVAQDSLTAAKAKHDAGAGTLSDQLQAQTSYRRAVLD
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
RVSAEGDVRNATGVLAVAMGLDANTPLRIATAEPPVDRNAFAEGIAQLIDEAKRQHPKLV
HHCCCCCCCCCCCCEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCHHH
AARAKLDAARANVDAVRAQGRPTISLTGSLSRNSPSYQQQPGIQTTASHGSMIGVQVTIP
HHHHHHHHHHCCHHHHHCCCCCEEEEEECCCCCCCCHHHCCCCCEECCCCCEEEEEEEEH
LFEGFASGYRVAAAQAQADAQEADVQNTELNVSLDVWKSYQSLQTDTANLDNSKDLLGDA
HHHHHHCCCEEEHHHHHCCHHHCCCCCCEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHH
QRSLDIARGRYKAGVGTFTELLNAQTALADAQKQRVQALSKWRTARLKLAASLGSIGLWS
HHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
AH
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2905265; 12910271 [H]