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The map label for this gene is penA [H]

Identifier: 237813925

GI number: 237813925

Start: 3574995

End: 3576839

Strand: Reverse

Name: penA [H]

Synonym: GBP346_A3704

Alternate gene names: 237813925

Gene position: 3576839-3574995 (Counterclockwise)

Preceding gene: 237813926

Following gene: 237813924

Centisome position: 87.27

GC content: 65.42

Gene sequence:

>1845_bases
ATGAAGCCCGCTTCGAAGCGCACGCAAGGCGTGAAGTTCGCATCGAGCCCGGTGCTGTCGGTTCATCTGCCGATGTGGCG
CTCGAAGCTTGTCGTGTTCATGCTGTTCATCGCTTTCGTCGCGCTCGCGATGCGCGCGTTCTGGATCCAGGGGCCGGGCA
ACGCGTTCTATCAGAAACAGGGCGAAAGCCGCTATCAGCGCACGCTCGAGTTGCCGGCGACGCGCGGCAAGATCCTCGAT
CGCAACGGCCTCGTGCTTGCGACGAGCTTGCCCGTGCGCGCGATCTGGGCGATTCCGGCCGACGTGCCCGACGACCTCGG
CGCGGACAAGCTCGACGCGCTCGGCAAGCTGCTCGGGATGACGACGAAGGAGCTGAAGGCGAAGCTCTCGGAAGACAAGA
CGTTCGTCTACGTGAAGCGCCAGGTGCCGCTCGACGTCGCCGAGCAGGTCGCGAAGCTCGACATTCCCGGCGTCTATCAG
CGCGGCGAATACAAGCGCTTCTACCCGGAAGGCGAGATCACCGCGCACCTGATCGGTTTCACGAACGTCGAGGACGAGGG
GCAGGAGGGCGTCGAGCTCGCCGACCAGAAGCTGCTCGTCGGCACGCCGGGCAGCCGCCACGTGATCAAGGACCGCGTCG
GGCACATCGTCGAGGACGTCGATGCGCAGGTGCCGCCGCACAACGGCGCCGACGTCGGCCTGTCGATCGACAGCAAGATC
CAGTACATCACGTATACGAACCTGAAGGCGGCCGTCGAGAAATTCCACGCGAAGGCGGGCGCGGCGATGGTGATCGACGT
GCAGACGGGCGAGGTGCTGTCACTCGTCAATTATCCGACGTATAACCCGAACGACCGCTCGCACCTGACGGGCGAGCAGT
TGCGCAACCGCGTGCTGACCGACGTGTTCGAGCCGGGCTCGATCATGAAGCCGTTCACGGTCTCGCTCGCGCTCGATCTG
CATCGCGTGACGCCGAACACGCTCGTCGAGACGGGCAATGGCCATTTCGTGCTCGACGGCGCGCCGATCACCGACGATTC
GGGCTTCGGCACGCTGACCGTCGGCGGCGTGATCCAGAAATCGAGCAACATCGGCGCGACGAAGATCGCGATGACGATGA
AGCCCGAGGAAATGTGGAATATGTATACAGGCATCGGGCTCGGACAGGCGCCGAAGGTCGGCTTTCCCGGCGCGGCGGCG
GGCCGCCTGCGCCCGTGGAAGAACTGGCGGCGCATCGAGCAGGCGACGATGTCGTACGGCTACGGCCTGTCGGTGTCGCT
GTTCCAGCTCGCGCGCGCATACACCGCGATCGCGCACGACGGCGAACTGATGCCCGTGACGATTTTCAAGACGAACGACG
CGCAACCCCCGACGGGCCCGCGCGTCTTCTCGCCGACGACGGCCCGCGAAGTGCGCACGATGCTCGAATCCGTCGTGTCG
CCGCAGGGTACGTCACCGAATGCGGCGGTGCCCGGCTATCGCGTCGGCGGCAAGAGCGGTACCGCTTACAAGCAGGTCGG
CCGCGGCTATGATCACAAGAAGTATCGCGCGTCGTTCGTCGGGATGGCGCCGATGCCGAACCCGCGCATCGTCGTTGCCG
TGTCGGTCGACGAACCGACGACGGGCGGTCACTTCGGCGGCCAGGTGTCGGGCCCCGTATTCTCCGCGATCGTCGGCGAT
ACGCTGCGCGCGCTGAACGTGCCGCCCAACATGCCGGTCAAGCAGCTCGTCGTGTCGGATGATGCGACGAACGCCGCCGC
CGCGCAAAAGCTGGCCGCGAATGCGCCGGCGAAACGCCTGATCGTGTCGAGCACGACACACAACCGTCCTGGAGTAGTTC
GATGA

Upstream 100 bases:

>100_bases
TCACGACGGGGCGCACGCAATACCTGACGCTTTCGCCCGGCGCGGCGAAGGCGGTCGACGCGCCGCTTCCCGCATCCGCC
GCGCCGACGGGAGGCGCGCG

Downstream 100 bases:

>100_bases
GCGCCGCGCGCAGTTCTCATCCGGCGCACCAACAGATCGCAGCCGCGCTCGCGTGGCTGCGCGCGCATGTCGTGCCCGGC
GCGCAGTTACACGCCGACAC

Product: penicillin-binding protein

Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 614; Mature: 614

Protein sequence:

>614_residues
MKPASKRTQGVKFASSPVLSVHLPMWRSKLVVFMLFIAFVALAMRAFWIQGPGNAFYQKQGESRYQRTLELPATRGKILD
RNGLVLATSLPVRAIWAIPADVPDDLGADKLDALGKLLGMTTKELKAKLSEDKTFVYVKRQVPLDVAEQVAKLDIPGVYQ
RGEYKRFYPEGEITAHLIGFTNVEDEGQEGVELADQKLLVGTPGSRHVIKDRVGHIVEDVDAQVPPHNGADVGLSIDSKI
QYITYTNLKAAVEKFHAKAGAAMVIDVQTGEVLSLVNYPTYNPNDRSHLTGEQLRNRVLTDVFEPGSIMKPFTVSLALDL
HRVTPNTLVETGNGHFVLDGAPITDDSGFGTLTVGGVIQKSSNIGATKIAMTMKPEEMWNMYTGIGLGQAPKVGFPGAAA
GRLRPWKNWRRIEQATMSYGYGLSVSLFQLARAYTAIAHDGELMPVTIFKTNDAQPPTGPRVFSPTTAREVRTMLESVVS
PQGTSPNAAVPGYRVGGKSGTAYKQVGRGYDHKKYRASFVGMAPMPNPRIVVAVSVDEPTTGGHFGGQVSGPVFSAIVGD
TLRALNVPPNMPVKQLVVSDDATNAAAAQKLAANAPAKRLIVSSTTHNRPGVVR

Sequences:

>Translated_614_residues
MKPASKRTQGVKFASSPVLSVHLPMWRSKLVVFMLFIAFVALAMRAFWIQGPGNAFYQKQGESRYQRTLELPATRGKILD
RNGLVLATSLPVRAIWAIPADVPDDLGADKLDALGKLLGMTTKELKAKLSEDKTFVYVKRQVPLDVAEQVAKLDIPGVYQ
RGEYKRFYPEGEITAHLIGFTNVEDEGQEGVELADQKLLVGTPGSRHVIKDRVGHIVEDVDAQVPPHNGADVGLSIDSKI
QYITYTNLKAAVEKFHAKAGAAMVIDVQTGEVLSLVNYPTYNPNDRSHLTGEQLRNRVLTDVFEPGSIMKPFTVSLALDL
HRVTPNTLVETGNGHFVLDGAPITDDSGFGTLTVGGVIQKSSNIGATKIAMTMKPEEMWNMYTGIGLGQAPKVGFPGAAA
GRLRPWKNWRRIEQATMSYGYGLSVSLFQLARAYTAIAHDGELMPVTIFKTNDAQPPTGPRVFSPTTAREVRTMLESVVS
PQGTSPNAAVPGYRVGGKSGTAYKQVGRGYDHKKYRASFVGMAPMPNPRIVVAVSVDEPTTGGHFGGQVSGPVFSAIVGD
TLRALNVPPNMPVKQLVVSDDATNAAAAQKLAANAPAKRLIVSSTTHNRPGVVR
>Mature_614_residues
MKPASKRTQGVKFASSPVLSVHLPMWRSKLVVFMLFIAFVALAMRAFWIQGPGNAFYQKQGESRYQRTLELPATRGKILD
RNGLVLATSLPVRAIWAIPADVPDDLGADKLDALGKLLGMTTKELKAKLSEDKTFVYVKRQVPLDVAEQVAKLDIPGVYQ
RGEYKRFYPEGEITAHLIGFTNVEDEGQEGVELADQKLLVGTPGSRHVIKDRVGHIVEDVDAQVPPHNGADVGLSIDSKI
QYITYTNLKAAVEKFHAKAGAAMVIDVQTGEVLSLVNYPTYNPNDRSHLTGEQLRNRVLTDVFEPGSIMKPFTVSLALDL
HRVTPNTLVETGNGHFVLDGAPITDDSGFGTLTVGGVIQKSSNIGATKIAMTMKPEEMWNMYTGIGLGQAPKVGFPGAAA
GRLRPWKNWRRIEQATMSYGYGLSVSLFQLARAYTAIAHDGELMPVTIFKTNDAQPPTGPRVFSPTTAREVRTMLESVVS
PQGTSPNAAVPGYRVGGKSGTAYKQVGRGYDHKKYRASFVGMAPMPNPRIVVAVSVDEPTTGGHFGGQVSGPVFSAIVGD
TLRALNVPPNMPVKQLVVSDDATNAAAAQKLAANAPAKRLIVSSTTHNRPGVVR

Specific function: Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=554, Percent_Identity=41.1552346570397, Blast_Score=400, Evalue=1e-112,
Organism=Escherichia coli, GI1786854, Length=585, Percent_Identity=25.1282051282051, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: 2.4.1.129

Molecular weight: Translated: 66361; Mature: 66361

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPASKRTQGVKFASSPVLSVHLPMWRSKLVVFMLFIAFVALAMRAFWIQGPGNAFYQKQ
CCCCCCCCCCCEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHCC
GESRYQRTLELPATRGKILDRNGLVLATSLPVRAIWAIPADVPDDLGADKLDALGKLLGM
CHHHHHHHHCCCCCCCCEECCCCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHCC
TTKELKAKLSEDKTFVYVKRQVPLDVAEQVAKLDIPGVYQRGEYKRFYPEGEITAHLIGF
CHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEEEEEEE
TNVEDEGQEGVELADQKLLVGTPGSRHVIKDRVGHIVEDVDAQVPPHNGADVGLSIDSKI
CCCCCCCCCCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCE
QYITYTNLKAAVEKFHAKAGAAMVIDVQTGEVLSLVNYPTYNPNDRSHLTGEQLRNRVLT
EEEEECCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHH
DVFEPGSIMKPFTVSLALDLHRVTPNTLVETGNGHFVLDGAPITDDSGFGTLTVGGVIQK
HHCCCCCCCCCEEEEEEEEEECCCCCCEEECCCCEEEECCCCCCCCCCCEEEEECCEEEC
SSNIGATKIAMTMKPEEMWNMYTGIGLGQAPKVGFPGAAAGRLRPWKNWRRIEQATMSYG
CCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
YGLSVSLFQLARAYTAIAHDGELMPVTIFKTNDAQPPTGPRVFSPTTAREVRTMLESVVS
CCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHC
PQGTSPNAAVPGYRVGGKSGTAYKQVGRGYDHKKYRASFVGMAPMPNPRIVVAVSVDEPT
CCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCHHHHHHEEECCCCCCCCEEEEEEECCCC
TGGHFGGQVSGPVFSAIVGDTLRALNVPPNMPVKQLVVSDDATNAAAAQKLAANAPAKRL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHEEECCCCCHHHHHHHHHHCCCHHEE
IVSSTTHNRPGVVR
EEECCCCCCCCCCC
>Mature Secondary Structure
MKPASKRTQGVKFASSPVLSVHLPMWRSKLVVFMLFIAFVALAMRAFWIQGPGNAFYQKQ
CCCCCCCCCCCEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCHHHHCC
GESRYQRTLELPATRGKILDRNGLVLATSLPVRAIWAIPADVPDDLGADKLDALGKLLGM
CHHHHHHHHCCCCCCCCEECCCCEEEEECCCEEEEEECCCCCCCCCCCHHHHHHHHHHCC
TTKELKAKLSEDKTFVYVKRQVPLDVAEQVAKLDIPGVYQRGEYKRFYPEGEITAHLIGF
CHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHCCCCCCCCCCCCCEECCCCCEEEEEEEE
TNVEDEGQEGVELADQKLLVGTPGSRHVIKDRVGHIVEDVDAQVPPHNGADVGLSIDSKI
CCCCCCCCCCHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCE
QYITYTNLKAAVEKFHAKAGAAMVIDVQTGEVLSLVNYPTYNPNDRSHLTGEQLRNRVLT
EEEEECCHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHH
DVFEPGSIMKPFTVSLALDLHRVTPNTLVETGNGHFVLDGAPITDDSGFGTLTVGGVIQK
HHCCCCCCCCCEEEEEEEEEECCCCCCEEECCCCEEEECCCCCCCCCCCEEEEECCEEEC
SSNIGATKIAMTMKPEEMWNMYTGIGLGQAPKVGFPGAAAGRLRPWKNWRRIEQATMSYG
CCCCCCEEEEEEECHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
YGLSVSLFQLARAYTAIAHDGELMPVTIFKTNDAQPPTGPRVFSPTTAREVRTMLESVVS
CCCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCCCEEECCCHHHHHHHHHHHHHC
PQGTSPNAAVPGYRVGGKSGTAYKQVGRGYDHKKYRASFVGMAPMPNPRIVVAVSVDEPT
CCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCHHHHHHEEECCCCCCCCEEEEEEECCCC
TGGHFGGQVSGPVFSAIVGDTLRALNVPPNMPVKQLVVSDDATNAAAAQKLAANAPAKRL
CCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHEEECCCCCHHHHHHHHHHCCCHHEE
IVSSTTHNRPGVVR
EEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol

Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3126399 [H]