Click here to switch to the map view.

The map label for this gene is murE [H]

Identifier: 237813924

GI number: 237813924

Start: 3573454

End: 3574998

Strand: Reverse

Name: murE [H]

Synonym: GBP346_A3703

Alternate gene names: 237813924

Gene position: 3574998-3573454 (Counterclockwise)

Preceding gene: 237813925

Following gene: 237813923

Centisome position: 87.23

GC content: 71.46

Gene sequence:

>1545_bases
ATGAGCGCCGCGCGCAGTTCTCATCCGGCGCACCAACAGATCGCAGCCGCGCTCGCGTGGCTGCGCGCGCATGTCGTGCC
CGGCGCGCAGTTACACGCCGACACGCGCAGCCTGCGGGCGGGCGACGTGTTCGTCGCCTATGCGGTCGACGGCGCGGACA
ATCGTCCGTTCATCGCCGATGCGCTCGCGCGCGGCGCGGCCGCCGTGCTGTATCAGCCCGAAGGGTTCCCGGCCGAAGGG
CTCGACTCCGCGACGTCGCTCGCCGTGCCCGCGCTCGACGCGCTCGCGGGCGACATCGCGAGCGGCTGGTACGGCGACCC
GAGCGATGCGATGTTCGCGGTCGGCGTGACCGGCACGAACGGCAAGACGTCGTGCACGCAATGGATCGCCGCCGCGCTCA
CCGCGCTGCGCGAGCCGTGCGCGGTGATCGGCACGCTCGGCAGCGGGATGCCCGGCCAGCTCGTGCACACCGGCTTCACG
ACGCCCGACGCGCCGCAACTGCAGCGCAGCCTCGCGCAACTGCGCGATGCGGGCGCGCGGGCGGTCGCGATGGAAGTTTC
GTCGCACGCGCTGCATCAAGGGCGCGTGAACGGCACCGCGTTCGACGTCGCGGTGTTCACGAACCTCACGCAGGATCACC
TCGATTACCACCGCACGTTCGAAGCCTACGAGGCGGCGAAGGCGAAGCTCTTCGCCTGGCGCGGGCTGCGCTGCGCGATC
GTCAACCGCGACGATCCGGCCGGCCGCCGCCTGCTCGCGAATCTCGCCGGGCGCGTGCGAACGATCGCGTACGGCATCGA
TTCGCCGACGCAGGCGGCCGACGCGGATCGCGAGCTCGTCGCGACGAACGTTCGCGCGACCGCGACGGGCACCGCGTTTC
GCGTGACGTCAGCGTGGGGCGAAGCGGACGTCGAGGTCGGCACGCTCGGCGCGTTCAACGTGAGCAATCTGCTCGCGGTG
CTCGGCACGCTGCTCGCGGCCGACGTGCCGTTCGACGCGGCGCTCGCCGAAATCGGCAAGCTCGAATCGGTCAACGGCCG
GATGCAGCGATTGGGTGGGCGGCTGCAGAACGACGAGCCGCTCGTCGTCGTCGATTACGCGCACACGCCGGACGCGCTCG
AGAAGACGCTCGACGCGCTGCGCCCGATCGCGCAGGCGCGCGGCGGCGAGCTCATCTGTATGTTCGGCTGCGGCGGCGAC
CGCGATGCGACCAAGCGCCCGCTGATGGGCGCGATCGCCGAGCGGCTCGCGGACAAGGTCGTCGTGACGAGCGACAACCC
GCGCAGCGAGGATCCGCAGAAGATCATCGACCAGATCGTCGCCGGCATGCGGGATGCCTCGGGCGCGCGCCGTGTCGAGG
ATCGCGCGAGCGCGATCCTGCAGGCGGTGCGCGGTGCGGCGCGCGAAGACGTCGTGCTGCTCGCCGGCAAGGGCCATGAG
GCGACGCAGGAAATCATGGGCAAGAAGCGCGCGTTCTCCGATCAGGACCACGCGCGCCTCGCGCTCGCCGCGCGCACGAC
GCAGACTCGCGGAGGCGGCGAATGA

Upstream 100 bases:

>100_bases
ATGCGACGAACGCCGCCGCCGCGCAAAAGCTGGCCGCGAATGCGCCGGCGAAACGCCTGATCGTGTCGAGCACGACACAC
AACCGTCCTGGAGTAGTTCG

Downstream 100 bases:

>100_bases
CGATGCTGACCTTGCGCGAAGCCGCTGCGATGATTCCCGGCGCGACCGTGCTCGGCGACGAGCGCGCGTCGTTCGAGCGG
GTGTCGACGGACAGCCGCTC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSAARSSHPAHQQIAAALAWLRAHVVPGAQLHADTRSLRAGDVFVAYAVDGADNRPFIADALARGAAAVLYQPEGFPAEG
LDSATSLAVPALDALAGDIASGWYGDPSDAMFAVGVTGTNGKTSCTQWIAAALTALREPCAVIGTLGSGMPGQLVHTGFT
TPDAPQLQRSLAQLRDAGARAVAMEVSSHALHQGRVNGTAFDVAVFTNLTQDHLDYHRTFEAYEAAKAKLFAWRGLRCAI
VNRDDPAGRRLLANLAGRVRTIAYGIDSPTQAADADRELVATNVRATATGTAFRVTSAWGEADVEVGTLGAFNVSNLLAV
LGTLLAADVPFDAALAEIGKLESVNGRMQRLGGRLQNDEPLVVVDYAHTPDALEKTLDALRPIAQARGGELICMFGCGGD
RDATKRPLMGAIAERLADKVVVTSDNPRSEDPQKIIDQIVAGMRDASGARRVEDRASAILQAVRGAAREDVVLLAGKGHE
ATQEIMGKKRAFSDQDHARLALAARTTQTRGGGE

Sequences:

>Translated_514_residues
MSAARSSHPAHQQIAAALAWLRAHVVPGAQLHADTRSLRAGDVFVAYAVDGADNRPFIADALARGAAAVLYQPEGFPAEG
LDSATSLAVPALDALAGDIASGWYGDPSDAMFAVGVTGTNGKTSCTQWIAAALTALREPCAVIGTLGSGMPGQLVHTGFT
TPDAPQLQRSLAQLRDAGARAVAMEVSSHALHQGRVNGTAFDVAVFTNLTQDHLDYHRTFEAYEAAKAKLFAWRGLRCAI
VNRDDPAGRRLLANLAGRVRTIAYGIDSPTQAADADRELVATNVRATATGTAFRVTSAWGEADVEVGTLGAFNVSNLLAV
LGTLLAADVPFDAALAEIGKLESVNGRMQRLGGRLQNDEPLVVVDYAHTPDALEKTLDALRPIAQARGGELICMFGCGGD
RDATKRPLMGAIAERLADKVVVTSDNPRSEDPQKIIDQIVAGMRDASGARRVEDRASAILQAVRGAAREDVVLLAGKGHE
ATQEIMGKKRAFSDQDHARLALAARTTQTRGGGE
>Mature_513_residues
SAARSSHPAHQQIAAALAWLRAHVVPGAQLHADTRSLRAGDVFVAYAVDGADNRPFIADALARGAAAVLYQPEGFPAEGL
DSATSLAVPALDALAGDIASGWYGDPSDAMFAVGVTGTNGKTSCTQWIAAALTALREPCAVIGTLGSGMPGQLVHTGFTT
PDAPQLQRSLAQLRDAGARAVAMEVSSHALHQGRVNGTAFDVAVFTNLTQDHLDYHRTFEAYEAAKAKLFAWRGLRCAIV
NRDDPAGRRLLANLAGRVRTIAYGIDSPTQAADADRELVATNVRATATGTAFRVTSAWGEADVEVGTLGAFNVSNLLAVL
GTLLAADVPFDAALAEIGKLESVNGRMQRLGGRLQNDEPLVVVDYAHTPDALEKTLDALRPIAQARGGELICMFGCGGDR
DATKRPLMGAIAERLADKVVVTSDNPRSEDPQKIIDQIVAGMRDASGARRVEDRASAILQAVRGAAREDVVLLAGKGHEA
TQEIMGKKRAFSDQDHARLALAARTTQTRGGGE

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=470, Percent_Identity=44.6808510638298, Blast_Score=309, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 54017; Mature: 53886

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAARSSHPAHQQIAAALAWLRAHVVPGAQLHADTRSLRAGDVFVAYAVDGADNRPFIAD
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCEEEEEEECCCCCCCHHHH
ALARGAAAVLYQPEGFPAEGLDSATSLAVPALDALAGDIASGWYGDPSDAMFAVGVTGTN
HHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCC
GKTSCTQWIAAALTALREPCAVIGTLGSGMPGQLVHTGFTTPDAPQLQRSLAQLRDAGAR
CCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCC
AVAMEVSSHALHQGRVNGTAFDVAVFTNLTQDHLDYHRTFEAYEAAKAKLFAWRGLRCAI
EEEEHHHHHHHHCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCEEEE
VNRDDPAGRRLLANLAGRVRTIAYGIDSPTQAADADRELVATNVRATATGTAFRVTSAWG
ECCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHCCEEEECCCEEEEECCCC
EADVEVGTLGAFNVSNLLAVLGTLLAADVPFDAALAEIGKLESVNGRMQRLGGRLQNDEP
CCCCEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHCCHHHHHHCCCCCCCCC
LVVVDYAHTPDALEKTLDALRPIAQARGGELICMFGCGGDRDATKRPLMGAIAERLADKV
EEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCE
VVTSDNPRSEDPQKIIDQIVAGMRDASGARRVEDRASAILQAVRGAAREDVVLLAGKGHE
EEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCH
ATQEIMGKKRAFSDQDHARLALAARTTQTRGGGE
HHHHHHHHHHCCCCCCHHEEEEEEECCCCCCCCC
>Mature Secondary Structure 
SAARSSHPAHQQIAAALAWLRAHVVPGAQLHADTRSLRAGDVFVAYAVDGADNRPFIAD
CCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCEEEEEEECCCCCCCHHHH
ALARGAAAVLYQPEGFPAEGLDSATSLAVPALDALAGDIASGWYGDPSDAMFAVGVTGTN
HHHCCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCC
GKTSCTQWIAAALTALREPCAVIGTLGSGMPGQLVHTGFTTPDAPQLQRSLAQLRDAGAR
CCHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHCCCC
AVAMEVSSHALHQGRVNGTAFDVAVFTNLTQDHLDYHRTFEAYEAAKAKLFAWRGLRCAI
EEEEHHHHHHHHCCCCCCEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCEEEE
VNRDDPAGRRLLANLAGRVRTIAYGIDSPTQAADADRELVATNVRATATGTAFRVTSAWG
ECCCCHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHCCEEEECCCEEEEECCCC
EADVEVGTLGAFNVSNLLAVLGTLLAADVPFDAALAEIGKLESVNGRMQRLGGRLQNDEP
CCCCEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHCCHHHHHHCCCCCCCCC
LVVVDYAHTPDALEKTLDALRPIAQARGGELICMFGCGGDRDATKRPLMGAIAERLADKV
EEEEEECCCCHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCE
VVTSDNPRSEDPQKIIDQIVAGMRDASGARRVEDRASAILQAVRGAAREDVVLLAGKGHE
EEECCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCH
ATQEIMGKKRAFSDQDHARLALAARTTQTRGGGE
HHHHHHHHHHCCCCCCHHEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]