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The map label for this gene is murD [H]

Identifier: 237813921

GI number: 237813921

Start: 3569327

End: 3570853

Strand: Reverse

Name: murD [H]

Synonym: GBP346_A3700

Alternate gene names: 237813921

Gene position: 3570853-3569327 (Counterclockwise)

Preceding gene: 237813922

Following gene: 237813920

Centisome position: 87.12

GC content: 72.56

Gene sequence:

>1527_bases
ATGTTTGGCGAGATGTTCGGAGATCGGCAGCGGCCGATGGTGCTCGTGCTGGGGCTCGGCGAATCGGGCCTCGCGATCGC
GCGGTGGTGCGCGAGGCACGGGTGCCGGTTGCGCGTCGCCGATACGCGCGAGACGCCGCCCAACCTGGCCGCGCTGACGG
CGGCGGGCGTCGATTTCGAATTCGTCGGCGGCGCGTTTTCGCCGGCGCTCGTCGACGGCGGGATCGAACTCGTCGCGCTG
AGCCCCGGCCTGTCGCCGCTCGCGGAGGATCTCGCGCCGCTCGTCGCCGCCGCGCGCGAGCGCGGCATTCCCGTGTGGGG
CGAGCTCGAATTCTTCGCGCAGGCGCTCGCGGCGCTCGGCGCGAACGGCTACGCGCCGAAGGTGATCGCGATCACCGGCA
CGAACGGCAAGACGACGACGACGAGCCTCGCGGGCCTGCTGTGCGAGCGCGCGGGCAAGAAGGTCGCGGTCGCGGGCAAC
ATCAGCCCGGCGATGCTCGACAAGCTGACCGAAGCGATCGACGCGGCGGCGCTGCCGGACGTGTGGGTGCTCGAGCTGTC
GAGCTTTCAGCTCGACACCGCGCACACGTTCGCGCCCGATGCGGCAACGATCCTCAACATCACGCAGGACCATCTCGACT
GGCACGGCGGCTTCGCCGCGTATGCGGCCGCGAAGGGCCGCGTCTTCGGGCCGCGCACGGTGCGCGTGCTCAATCGCGAC
GATGCCGAGGTGATGCGCTTCGCGCCGCCCGCCGCGGCCGCCGACGCGCCGCGCGCGGTCACGTTCGGCCTGAACGAGCC
GGCCGCCGACGGCGATTACGGCCTGCTGCGCGAAAACGGCATCGCATGGCTCGTCGAGGCGATCGATCGCGACGGGGCCG
ATGCGCCCGCCGCGCCGTCACGCCGGCGCAAGCAGGAGGCGGCGAATCCGCCCGACATCGCGCTCAAGCGGCTGATGCCC
GCCGACGCGTTGCGCATCCGCGGGCTGCACAACGCGGCGAACGCGCTCGCCGCCTATGCGCTCGCGCGCGCGATCGGCCT
GCCGGCCGCGCCGCTCCTGCACGGCTTGCGCGAATACCGCGGCGAGCCGCACCGCGTCGAAGTGATCGCGACGCTCGACG
GCGTCGACTATGTCGACGACAGCAAGGGCACGAACGTCGGCGCGACGGTCGCGGCGCTCGACGGCCTCGCGCAGCGTGCG
GTGCTGATCGCGGGCGGCGACGGCAAGGGGCAGGATTTCGAGCCGCTCGCGACGCCCGTCGCGCGCTGGTGCCGCGCGGT
GATGCTGATCGGCCGCGACGCGCCCGCGTTGCGCGAGGCGCTTGCCGACACGGGCGTGCCGCTTGCCGATCACGCGACGC
TCGAAGCCGCGGTGCGCGCGGCGAGCGCGCTCGCGCAGCCGGGGGACGCGGTGCTGCTGTCGCCCGCGTGCGCGAGCCTC
GACATGTTCCGCAATTACGCACACCGGGCCGACGTGTTCCGCAGCGCGGTCGAAGATATCGCCTTGGAAAAAGGAACGAC
GCTATGA

Upstream 100 bases:

>100_bases
TGGAAGGAAACGCAGGTGGTGGTGCGTTTCTGGATCATCACGTTGATGCTGTGCCTGTTCGGTTTGTCCACCCTCAAGCT
GCGGTAAAGGGAAGGTAAGG

Downstream 100 bases:

>100_bases
GCTGGTCCGATCGCCTCGTTTCCCGTTTCAACGGCGTCCGCGACGCGGGCGGCGCCGCCGTGCCGCGCACCGCCGCACGG
GCGGCCTCGGGCGCGCGCGC

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 508; Mature: 508

Protein sequence:

>508_residues
MFGEMFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRVADTRETPPNLAALTAAGVDFEFVGGAFSPALVDGGIELVAL
SPGLSPLAEDLAPLVAAARERGIPVWGELEFFAQALAALGANGYAPKVIAITGTNGKTTTTSLAGLLCERAGKKVAVAGN
ISPAMLDKLTEAIDAAALPDVWVLELSSFQLDTAHTFAPDAATILNITQDHLDWHGGFAAYAAAKGRVFGPRTVRVLNRD
DAEVMRFAPPAAAADAPRAVTFGLNEPAADGDYGLLRENGIAWLVEAIDRDGADAPAAPSRRRKQEAANPPDIALKRLMP
ADALRIRGLHNAANALAAYALARAIGLPAAPLLHGLREYRGEPHRVEVIATLDGVDYVDDSKGTNVGATVAALDGLAQRA
VLIAGGDGKGQDFEPLATPVARWCRAVMLIGRDAPALREALADTGVPLADHATLEAAVRAASALAQPGDAVLLSPACASL
DMFRNYAHRADVFRSAVEDIALEKGTTL

Sequences:

>Translated_508_residues
MFGEMFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRVADTRETPPNLAALTAAGVDFEFVGGAFSPALVDGGIELVAL
SPGLSPLAEDLAPLVAAARERGIPVWGELEFFAQALAALGANGYAPKVIAITGTNGKTTTTSLAGLLCERAGKKVAVAGN
ISPAMLDKLTEAIDAAALPDVWVLELSSFQLDTAHTFAPDAATILNITQDHLDWHGGFAAYAAAKGRVFGPRTVRVLNRD
DAEVMRFAPPAAAADAPRAVTFGLNEPAADGDYGLLRENGIAWLVEAIDRDGADAPAAPSRRRKQEAANPPDIALKRLMP
ADALRIRGLHNAANALAAYALARAIGLPAAPLLHGLREYRGEPHRVEVIATLDGVDYVDDSKGTNVGATVAALDGLAQRA
VLIAGGDGKGQDFEPLATPVARWCRAVMLIGRDAPALREALADTGVPLADHATLEAAVRAASALAQPGDAVLLSPACASL
DMFRNYAHRADVFRSAVEDIALEKGTTL
>Mature_508_residues
MFGEMFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRVADTRETPPNLAALTAAGVDFEFVGGAFSPALVDGGIELVAL
SPGLSPLAEDLAPLVAAARERGIPVWGELEFFAQALAALGANGYAPKVIAITGTNGKTTTTSLAGLLCERAGKKVAVAGN
ISPAMLDKLTEAIDAAALPDVWVLELSSFQLDTAHTFAPDAATILNITQDHLDWHGGFAAYAAAKGRVFGPRTVRVLNRD
DAEVMRFAPPAAAADAPRAVTFGLNEPAADGDYGLLRENGIAWLVEAIDRDGADAPAAPSRRRKQEAANPPDIALKRLMP
ADALRIRGLHNAANALAAYALARAIGLPAAPLLHGLREYRGEPHRVEVIATLDGVDYVDDSKGTNVGATVAALDGLAQRA
VLIAGGDGKGQDFEPLATPVARWCRAVMLIGRDAPALREALADTGVPLADHATLEAAVRAASALAQPGDAVLLSPACASL
DMFRNYAHRADVFRSAVEDIALEKGTTL

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=493, Percent_Identity=38.5395537525355, Blast_Score=235, Evalue=6e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 53044; Mature: 53044

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGEMFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRVADTRETPPNLAALTAAGVDFE
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCEE
FVGGAFSPALVDGGIELVALSPGLSPLAEDLAPLVAAARERGIPVWGELEFFAQALAALG
ECCCCCCCHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
ANGYAPKVIAITGTNGKTTTTSLAGLLCERAGKKVAVAGNISPAMLDKLTEAIDAAALPD
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCC
VWVLELSSFQLDTAHTFAPDAATILNITQDHLDWHGGFAAYAAAKGRVFGPRTVRVLNRD
EEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCEECCCEEEEECCC
DAEVMRFAPPAAAADAPRAVTFGLNEPAADGDYGLLRENGIAWLVEAIDRDGADAPAAPS
CHHHHEECCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCHHHHHHHHCCCCCCCCCCCH
RRRKQEAANPPDIALKRLMPADALRIRGLHNAANALAAYALARAIGLPAAPLLHGLREYR
HHHHHHCCCCHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
GEPHRVEVIATLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIAGGDGKGQDFEPLATPV
CCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHH
ARWCRAVMLIGRDAPALREALADTGVPLADHATLEAAVRAASALAQPGDAVLLSPACASL
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHH
DMFRNYAHRADVFRSAVEDIALEKGTTL
HHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MFGEMFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRVADTRETPPNLAALTAAGVDFE
CCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCEE
FVGGAFSPALVDGGIELVALSPGLSPLAEDLAPLVAAARERGIPVWGELEFFAQALAALG
ECCCCCCCHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHC
ANGYAPKVIAITGTNGKTTTTSLAGLLCERAGKKVAVAGNISPAMLDKLTEAIDAAALPD
CCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCCC
VWVLELSSFQLDTAHTFAPDAATILNITQDHLDWHGGFAAYAAAKGRVFGPRTVRVLNRD
EEEEEECCEEECCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCCEECCCEEEEECCC
DAEVMRFAPPAAAADAPRAVTFGLNEPAADGDYGLLRENGIAWLVEAIDRDGADAPAAPS
CHHHHEECCCCCCCCCCCEEEECCCCCCCCCCCCEECCCCHHHHHHHHCCCCCCCCCCCH
RRRKQEAANPPDIALKRLMPADALRIRGLHNAANALAAYALARAIGLPAAPLLHGLREYR
HHHHHHCCCCHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
GEPHRVEVIATLDGVDYVDDSKGTNVGATVAALDGLAQRAVLIAGGDGKGQDFEPLATPV
CCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCEEEEECCCCCCCCCCHHHHHH
ARWCRAVMLIGRDAPALREALADTGVPLADHATLEAAVRAASALAQPGDAVLLSPACASL
HHHHHHHHHHCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCHHHHH
DMFRNYAHRADVFRSAVEDIALEKGTTL
HHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA